Studies of the Toxicological Potential of Tripeptides (L-Valyl-L-prolyl-L-proline and L-lsoleucyl-L-prolyl-L-proline): VII. Micronucleus Test of Tripeptides-Containing Casein Hydrolysate and Lactobacillus helveticus-fermented Milk Powders in Rats and Mice

2005 ◽  
Vol 24 (4_suppl) ◽  
pp. 91-96 ◽  
Author(s):  
Keiichi Matsuura ◽  
John H. Mennear ◽  
Masafumi Maeno ◽  
Bruce K. Bernard
Keyword(s):  
Bone Marrow Cells ◽  
Micronucleus Test ◽  
Casein Hydrolysate ◽  
Fermented Milk ◽  
Positive Control ◽  
Lactobacillus Helveticus ◽  
Oral Gavage ◽  
Mutagenic Potential ◽  
Polychromatic Erythrocytes ◽  
Rats And Mice

The objective of these in vivo experiments was to assess the mutagenic potential of tripeptides as reflected by the ability of the test compound to induce the formation of micronuclei in mouse polychromatic erythrocytes. The test agents used in these experiments were (1) powdered Aspergillus oryzae protease casein hydrolysate (CH) and (2) powdered Lactobacillus helveticus-fermented milk (FM). Both test agents contain two tripeptides, L-valyl-L-prolyl-L-proline (VPP) and L-isoleucyl-L-prolyl-L-proline (IPP). Male Sprague-Dawley rats (five per group) were administered doses of 0, 500, 1000, or 2000 mg (0, 3, 6, or 12 mg VPP plus IPP)/kg body weight (BW)/day CH by oral gavage for 2 days. Male CD-I mice (six per group) received a single oral gavage dose of 0, 500, 1000, or 2000 mg (0, 0.8, 1.6 or 3.3 mg VPP plus IPP)/kg BW of FM. Positive-control agents were cyclophosphamide (10 mg/kg, intraperitoneal [i.p.]) in rats and mitocycin C (2 mg/kg, i.p.) in mice. Twenty-four hours after the second dose of CH, or the dose of cyclophosphamide to rats, or FM or mitocycin C to mice, bone marrow cells were fixed and examined for the presence of polychromatic erythrocytes (PCEs) and the presence or absence of mi-cronucleated PCEs (MNPCEs). Administration of CH to rats and FM to mice produced neither changes in body weights nor signs of systemic toxicity. Similarly, neither CH nor FM caused statistically significant variations in the incidences of either PCEs or MNPCEs. Both positive-control agents caused unequivocal increases in the incidence of MNPCEs and cyclophosphamide significantly reduced the percent of rat erythrocytes appearing as PCEs. The results of these micronucleus assays conducted with either powdered CH or FM in rats and mice, respectively, show that neither form of the tripeptides possesses the potential to induce micronuclei formation in these rodent species.

Studies of the Toxicological Potential of Tripeptides (L-Valyl-L-prolyl-L-proline and L-lsoleucyl-L-prolyl-L-proline): VIII. Assessment of Cytotoxicity and Clastogenicity of Tripeptides-Containing Casein Hydrolysate and Lactobacillus helveticus-fermented Milk Powders in Chinese Hamster Lung Cells

2005 ◽  
Vol 24 (4_suppl) ◽  
pp. 97-105 ◽  
Author(s):  
Masafumi Maeno ◽  
Seiichi Mizuno ◽  
John H. Mennear ◽  
Bruce K. Bernard
Keyword(s):  
Mitomycin C ◽  
Chromosomal Aberrations ◽  
Chinese Hamster ◽  
Casein Hydrolysate ◽  
Fermented Milk ◽  
Positive Control ◽  
Lactobacillus Helveticus ◽  
Continuous Exposure ◽  
Short Term ◽  
Mutagenic Potential

The objective of this chromosomal aberration test was to assess the mutagenic potential of tripeptides by determining their ability to induce chromosomal aberrations in cultured Chinese hamster lung (CHL) cells. The test agents used in these experiments were (1) powdered casein hydrolysate (CH) and (2) powdered Lactobacillus helveticus-fermented milk (FM). Both test agents contain two tripeptides, L-valyl-L-prolyl-L-proline (VPP) and L-isoleucyl-L-prolyl-L-proline (IPP). CHL cells were cultured and exposed in the presence or absence of a rat hepatic metabolizing system (S9); CH or FM (1250, 2500, 5000 /μg/ml of incubation medium); or positive-control agents, mitomycin C (0.1 or 0.05 /ig/ml) or benzo(a)pyrene (20 /μg/ml). In experiments with CH, cells were incubated for 6 h (either in the presence or absence of S9) or for 24 h (without S9). In experiments with FM, the cells were incubated for 6 h (either in the presence or absence of S9) or for 24 or 48 h (without S9). Neither short-term nor continuous exposure to either CH or FM caused the induction of significant changes in cell growth indices, incidences of chromosomal aberrations or the incidence of polyploids. Exposures of cells to mitomycin C and benzo(a)pyrene consistently resulted in the induction of the anticipated aberrant cells after either short-term or continuous exposures. The results of these assays support the conclusions that, under the conditions of these experiments, neither CH nor FM possesses demonstrable potential for the induction of cytotoxicity or clastogenesis.

scholarly journals Antimutagenic Effect ofHibiscus sabdariffaL. Aqueous Extract on Rats Treated with Monosodium Glutamate

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Ana Carla Guidini Valentini Gheller ◽  
Jacqueline Kerkhoff ◽  
Gerardo Magela Vieira Júnior ◽  
Kleber Eduardo de Campos ◽  
Marina Mariko Sugui
Keyword(s):  
Aqueous Extract ◽  
High Performance ◽  
Micronucleus Test ◽  
Monosodium Glutamate ◽  
Positive Control ◽  
Control Group ◽  
Potential Candidate ◽  
Mato Grosso ◽  
Polychromatic Erythrocytes ◽  
Male Wistar Rats

Hibiscus sabdariffaL. is a plant of the Malvaceae family, commonly known as roselle.H. sabdariffais known to contain antioxidant, cholesterol-lowering, antiobesity, insulin resistance reduction, antihypertensive, and skin cancer chemopreventive properties. This study evaluated the effects ofH. sabdariffaaqueous extract against cyclophosphamide (CPA, 25 mg/Kg) induced damage to DNA in male Wistar rats by micronucleus test. Samples ofH. sabdariffacalyx were obtained in the municipality of Barra do Garças, Mato Grosso, Brazil. The aqueous extract was prepared by infusion and each animal received a daily dose of 400 mg/Kg by gavage for 15 consecutive days of treatment. The presence of anthocyanins was confirmed by ferric chloride test and phenolic compounds using high-performance liquid chromatography, with emphasis on the identification of rutin. The animals were sacrificed by deepening of anaesthesia to obtain bone marrow and determination of the frequency of micronucleated polychromatic erythrocytes. The group treated with the aqueous extract ofH. sabdariffarevealed a 91% reduction in micronucleus frequency when compared with the positive control group. Under the conditions tested,H. sabdariffaL. presented a protective effect to CPA-induced damage to DNA of the treated animals, and it is a potential candidate as a chemopreventive agent against carcinogenesis.

scholarly journals Evaluation of the mutagenic effect of the iodinated contrast medium Urografina® 292 using the micronucleus test in mouse bone marrow cells

2013 ◽  
Vol 85 (2) ◽  
pp. 737-744 ◽  
Author(s):  
MONICA B.B. BELLE ◽  
DANIELA D. LEFFA ◽  
DALIANE MAZZORANA ◽  
VANESSA M. DE ANDRADE
Keyword(s):  
Bone Marrow ◽  
Contrast Media ◽  
Contrast Medium ◽  
Bone Marrow Cells ◽  
Micronucleus Test ◽  
Control Group ◽  
Mouse Bone Marrow ◽  
Iodinated Contrast ◽  
Polychromatic Erythrocytes ◽  
Marrow Cells

Contrast media (CM) are frequently used in diagnostic radiology and in radiotherapy as a diagnostic tool and in treatment planning. Previous studies have demonstrated that these compounds induce chromosomal aberrations. This study evaluates the mutagenic effects induced by the contrast medium Urografina® 292 (meglumine amidotrizoate and sodium-ionic dimmer) in bone marrow cells (BMC) of mice in vivo. Micronuclei assay was performed in BMC of CF-1 mice injected with CM 1.5 and 3.0 mL/kg intravenous doses and 1.0, 2.0, 3.0 mL/kg intraperitoneal doses. The animals were beheaded 24 h after treatment by cervical dislocation, and femur BMC from each animal were used in the micronucleus test. The group treated with the highest intravenous injection of Urografina® 292 (3.0 mL/kg) presented an increase in the frequency of micronucleated polychromatic erythrocytes (MNPCEs) in relation at the control group (P<0.05). The results obtained after intraperitoneal administration of CM showed that all doses (1.0 mL/kg, 2.0 mL/kg and 3.0 mL/kg) increased the frequency of MNPCEs, being significantly different from the negative control (P< 0.01). The present results suggest that iodinated contrast media Urografina® 292 may cause a significant increase of cytogenetic damage in bone marrow cells of mice.

scholarly journals ESBLs (Extended Spectrum beta Lactamase) DDSM: (Double Disc Synergy Method) NCCL: (National Committee for Clinical Laboratory Standards

2019 ◽  
Vol 24 (7) ◽  
pp. 33
Author(s):  
WAGDI SABEEH SADEQ ◽  
SHIREEN ABED AL-RAZAQ TAHA
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Micronucleus Test ◽  
Negative Control ◽  
Toxic Effects ◽  
National Committee ◽  
Alcohol Extract ◽  
Polychromatic Erythrocytes ◽  
Mean Differences ◽  
Marrow Cells

Genotoxicity and cytotoxicity of Belomycin (BLM) have been evaluated in bone-marroww cells by micronucleus test, as well as the analysis of sperm shape abnormalities in male white mice, considering that BLM is the most wide anticancer drug used with patients. Also, the study includes assessment the effect of crude water and alcoholic extracts of the four o'clock flowers (Mirabilis jalapa Linn) in reducing BLM toxicity and the study was carried out  in the Genetics Laboratory of the Department of biology for the period from 1-10-2017 to 1-5-2019.So the genotoxicity and cytotoxicity were evaluated independently and in conjunction between two different dosages of BLM 0.8 and 1.6 mg.kg-1.bwt. and three orally dosage of different concentration of crud extracts, which is 39.8, 26.52, 13.26 mg.kg-1 and 7.02, 4.68, 2.34 mg.kg-1 o water and alcohol extract respectively. The results of assessment of BLM genotoxic effects showed that the drug caused induction of micronuclei, here were significant increase in micronucleated polychromatic erythrocytes (MNIPCEs) and significant increase in micronuclei(MNI) in the groups treated with 0.8 and 1.6 mg.kg-1 of BLM, compare to negative control at the level of significance P <0.05 On the other hand, the results showed that BLM has potential to induce sperm shape abnormalities, which include head and tail abnormalities, It included an increase in the proportion of morphological abnormalities in the head and tail of the sperm when compared to negative control at the significant level of P <0.05. The results also showed, that treatment with low dosages of four o'clock flower crud extracts didn’t induce neither micronuclei or any increase in PCEs numbers nor sperm shape abnormalities, although some toxic effects do exist with the higher dosages. Evaluation of results from dependent treatments of BLM and different concentrations of water and alcoholic crud extracts, we observed significant role of these extracts in reducing toxic effects of the drug BLM in bone marrow cells, which caused significant decrease in mean differences of MNIPCEs and MNI. More over the results showed significant decrease in mean differences of sperm shape and tail abnormalities compared to negative control. Results of the current study suggest that water and alcoholic four o'clock flower crud extracts have a role in reducing genotoxic and cytotoxic effects of BLM in bone-marrow cells and sperms of white mice   http://dx.doi.org/10.25130/tjps.24.2019.126

Fipronil-induced genotoxicity and DNA damage in vivo: Protective effect of vitamin E

2016 ◽  
Vol 36 (5) ◽  
pp. 508-519 ◽  
Author(s):  
PC Badgujar ◽  
NA Selkar ◽  
GA Chandratre ◽  
NN Pawar ◽  
VD Dighe ◽  
...  
Keyword(s):  
Dna Damage ◽  
Vitamin E ◽  
Environmental Protection Agency ◽  
Bone Marrow Cells ◽  
Micronucleus Test ◽  
Female Rats ◽  
Limited Information ◽  
Male And Female ◽  
Male And Female Rats ◽  
Polychromatic Erythrocytes

Fipronil, an insecticide of the phenylpyrazole class has been classified as a carcinogen by United States Environmental Protection Agency, yet very limited information is available about its genotoxic effects. Adult male and female animals were gavaged with various doses of fipronil (2.5, 12.5, and 25 mg/kg body weight (bw)) to evaluate micronucleus test (mice), chromosome aberration (CA), and comet assay (rats), respectively. Cyclophosphamide (40 mg/kg bw; intraperitoneal) was used as positive control. Another group of animals were pretreated with vitamin E orally (400 mg/kg bw) for 5 days prior to administration of fipronil (12.5 mg/kg). Fipronil exposure in both male and female mice caused significant increase in the frequency of micronuclei (MN) in polychromatic erythrocytes. Similarly, structural CAs in bone marrow cells and DNA damage in the lymphocytes was found to be significantly higher in the male and female rats exposed to fipronil as compared to their respective controls. The average degree of protection (male and female animals combined together) shown by pretreatment of vitamin E against fipronil-induced genotoxicity was 63.28%: CAs; 47.91%: MN formation; and 74.70%: DNA damage. Findings of this study demonstrate genotoxic nature of fipronil regardless of gender effect and documents protective role of vitamin E.

Studies of the Toxicological Potential of Tripeptides (L-Valyl-L-prolyl-L-proline and L-lsoleucyl-L-prolyl-L-proline): III. Single- and/or Repeated-Dose Toxicity of Tripeptides-Containing Lactobacillus helveticus-Fermented Milk Powder and Casein Hydrolysate in Rats

2005 ◽  
Vol 24 (4_suppl) ◽  
pp. 13-23 ◽  
Author(s):  
Masafumi Maeno ◽  
Yasunori Nakamura ◽  
John H. Mennear ◽  
Bruce K. Bernard
Keyword(s):  
Body Weight ◽  
Single Dose ◽  
Clinical Chemistry ◽  
Casein Hydrolysate ◽  
Fermented Milk ◽  
Lactobacillus Helveticus ◽  
Female Rats ◽  
Repeated Dose ◽  
Male And Female ◽  
Dose Study

The objective of these studies was to assess the toxicological potential of orally administered tripeptides in rats. The studies employed powdered L-valyl-L-prolyl-L-proline (VPP)- and L-isoleucyl-L-prolyl-L-proline (IPP)-containing test articles, including (1) powdered Lactobacillus helveticus-fermented milk (FM), (2) pasteurized casein hydrolysate (CH) generated by Aspergillus oryzae protease, and (3) synthesized VPP. All test articles were administered by oral gavage to male and female Sprague-Dawley rats. Specific goals of the single-dose and repeated-dose studies were to (1) identify doses that produce evidence of systemic and/or local (i.e., gastrointestinal) toxicity (e.g., lowest-observable-effect level [LOEL]); (2) estimate the maximally tolerated oral dose (MTD) ; and (3) identify specific target organs for toxicity of these tripeptides. Single doses of CH (2000 mg/kg), powdered FM (2000 or 4000 mg/kg), or VPP (40, 200, or 400 mg/kg) were administered 14 days prior to study termination. No treatment regimen caused either antemortem (gross observations, body weight, and food consumption parameters) or postmortem (necropsy) evidence of either systemic or local toxicity. In the repeated-dose study, powdered FM (0, 500, 1000, or 2000 mg/kg body weight [BW]/day) was administered by gastric gavage to male and female rats for 28 consecutive days. Antemortem evaluative parameters included gross observations, ophthalmic examinations, and clinical pathology (clinical chemistry, hematology, and urinalysis). Post mortem parameters included necropsy, determination of organ weights, and microscopic examination of major organs. There was neither in-life nor postmortem evidence that powdered FM administration caused physiological or toxicological changes. Under the conditions of these experiments, the single-dose LOEL of powdered FM, CH, and VPP were found to be greater than 4000,2000, and 400 mg/kg, respectively. The results of the repeated-dose study do not support identification of a target organ for powdered FM toxicity. Similarly, there was no evidence to support establishment of either the LOEL or MTD; both being greater than 2000 mg/kg/day for up to 28 consecutive days.

Genotoxic Effect of Epirubicin in Mouse Bone Marrow in vivo

2010 ◽  
Vol 65 (3-4) ◽  
pp. 211-217 ◽  
Author(s):  
Asuman K. Sen ◽  
Emin Karakas ◽  
Rahmi Bilaloglu
Keyword(s):  
Bone Marrow ◽  
Anticancer Drug ◽  
Bone Marrow Cells ◽  
Micronucleus Test ◽  
Negative Control ◽  
Genotoxic Effect ◽  
Mouse Bone Marrow ◽  
Polychromatic Erythrocytes ◽  
Marrow Cells

The genotoxic effect of epirubicin, a semisynthetic anthracycline antibiotic which has been used as an anticancer drug, was investigated in vivo on bone marrow cells of Swiss albino mice using the micronucleus test. To determine the incidence of micronuclei, mice were injected intraperitoneally with the drug at single doses of 4, 6, 8, and 10 mg/kg body weight. Then, bone marrow was sampled 18, 24, 36, and 48 h after the treatment. Polychromatic and normochromatic erythrocytes were examined for the presence of micronuclei. Epirubicin significantly increased the frequency of micronucleated polychromatic erythrocytes (MNPCEs) for all treatment periods compared with the negative control (P < 0.001). The frequency of MNPCEs increased with the dose, but at the highest dose used (which is considered to be quite toxic), the frequency of MNPCEs was rather lower. Epirubicin also decreased the ratio of polychromatic to normochromatic erythrocytes (PCE/NCE) for all sampling intervals, which is indicative of bone marrow cytotoxicity. It can be concluded from the present study that the anticancer drug epirubicin has genotoxic effects on mouse bone marrow cells.

scholarly journals Genotoxicity of Momordica charantia Extract in Swiss Albino Mice (Mus musculus)

2019 ◽  
Vol 3 (1) ◽  
pp. 62-68
Author(s):  
O. O. Adewumi ◽  
E. O. Oladele ◽  
I. A. Taiwo
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Warning Signal ◽  
Momordica Charantia ◽  
Control Group ◽  
Control Groups ◽  
Mutagenic Potential ◽  
Polychromatic Erythrocytes ◽  
Daily Dose ◽  
Albino Mice

The study aims at determining the safety of Momordica charantia (Bitter melon) for use by diabetic patient. This study was carried out to ascertain the genotoxic potential of Momordica charantia in mice using the micronucleus assays. A total of forty (40) laboratory albino mice weighing between 20 and 25 grams were obtained from the Zoological garden, University of Lagos. The mice were in eight groups comprising of five animals each. The doses of the extract administered were 50 mg/kg, 100 mg/kg and 150 mg/kg per body weight and the route of administration was oral by gastric gavages using a metal canula. The control groups A and B were fed with distilled water for 14 and 28 days respectively. The other groups were also treated with a daily dose of the extract for 14 and 28 days at different concentrations. The mutagenic potential of Momordica charantia was assessed with reference to the frequency of micronucleated polychromatic erythrocytes (MNPCE) in polychromatic erythrocytes (PCE) in the bone marrow of mice. During sacrifice, the bone marrow cells were collected from the femur and smeared on slides. For each mouse, polychromatic erythrocytes (PCE) were scored for the number of micronucleated polychromatic erythrocytes (MNPCE) and the percentage was calculated. The frequencies of MNPCE/PCE were 0.17 ± 0.09% and 0.13 ± 0.02% in the control group for 14 days and 28 days respectively, 0.33 ± 0.12% and 0.30 ± 0.08% in the 50 mg/kg group, 0.38 ± 0.06% and 0.34 ± 0.09% in the 100 mg/kg group, and 0.24 ± 0.08% and 0.35 ± 0.05% in the 150 mg/kg group. The results showed a significantly increased frequency of micronucleated polychromatic erythrocytes for the three doses administered. The results were considered statistically significant at p < 0.05. This indicates a warning signal to careless and indiscriminate use of the drug by humans.

scholarly journals Assessment of the mutagenic, antimutagenic and cytotoxic activities of ethanolic extract of araticum (Annona crassiflora Mart. 1841) by micronucleus test in mice

2008 ◽  
Vol 68 (1) ◽  
pp. 141-147 ◽  
Author(s):  
JB. Vilar ◽  
FL. Ferreira ◽  
PH. Ferri ◽  
LA. Guillo ◽  
L. Chen Chen
Keyword(s):  
Micronucleus Test ◽  
Mutagenic Activity ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Control Group ◽  
Cytotoxic Activities ◽  
Antimutagenic Activity ◽  
Polychromatic Erythrocytes ◽  
Significant Difference ◽  
Annona Crassiflora

A typical Brazilian plant, araticum (Annona crassiflora Mart.), is widely used in humans as therapeutic medicine to treat several diseases such as diarrhea, rheumatism and syphilis. It contains acetogenins which present cytotoxic, antitumogenic, and antiparasitic properties. In this study, mutagenic, antimutagenic and cytotoxic effects of araticum leaves ethanolic extract were evaluated by micronucleus test in mice. To evaluate the mutagenic activity, animals were treated with ethanolic extract of araticum (EEA) using 10, 20, 50, 100 and 160 mg.kg-1. For all doses, micronucleated polychromatic erythrocytes (MNPCE) frequency was evaluated at 24, 48 and 72 hours after treatment. To evaluate the antimutagenic activity, animals were treated with 10, 20, 50 and 100 mg.kg-1 of EEA and 4 mg.kg-1 of MMC simultaneously. The frequency of MNPCE was evaluated 36 hours after exposure. Cytotoxicity was evaluated by the polychromatic and normochromatic erythrocytes ratio (PCE/NCE). In the mutagenicity assessment, all doses of EEA resulted in no significant increase of MNPCE (P > 0.05), compared to solvent- control group. Regarding administration time, no significant difference among three evaluation periods was observed (P > 0.05). Such results indicate that EEA did not exert mutagenic activity. Cytotoxicity was evident in doses of 50, 100 and 160 mg.kg-1 at 24 and 48 hours after exposure. Concerning antimutagenicity, except the 10 mg.kg-1 co-administered with 4 mg/kg of MMC, all doses reduced significantly the frequency of MNPCE compared to the positive control group (P < 0.05). These results, therefore, indicate an antimutagenic activity of the EEA. Cytotoxicity was significantly increased (P < 0.01) at 100 mg.kg-1 EEA doses co-administered with 4 mg.kg-1 of MMC.

scholarly journals Assessment of the mutagenic and antimutagenic activity of Synadenium umbellatum Pax latex by micronucleus test in mice

2011 ◽  
Vol 71 (1) ◽  
pp. 169-174 ◽  
Author(s):  
PR. Melo-Reis ◽  
LSA. Bezerra ◽  
MAAB. Vale ◽  
RFR. Canhête ◽  
L. Chen-Chen
Keyword(s):  
Mitomycin C ◽  
Bone Marrow Cells ◽  
Malignant Tumors ◽  
Micronucleus Test ◽  
Negative Control ◽  
Control Group ◽  
Antimutagenic Activity ◽  
Experimental Conditions ◽  
Tropical Regions ◽  
Polychromatic Erythrocytes

Synadenium umbellatum Pax, popularly known as "cola-nota", is a medicinal plant that grows in tropical regions. The latex of this plant is used against various diseases, such as diabetes mellitus, leprosy, tripanosomiasis, leukemia, and several malignant tumors. The mutagenic, antimutagenic, and cytotoxic effects of the latex of this plant were investigated by measuring the frequency of micronuclei in mice bone marrow cells. To evaluate mutagenicity, the animals were treated with four doses of latex (10, 30, 50, and 100 mg/kg body weight). To study the antimutagenic activity, the animals were simultaneously treated with latex and mitomycin C (4 mg/kg). The cytotoxicity was evaluated by polychromatic and normochromatic erythrocytes ratio. Our results showed a significant increase of frequency of micronucleated polychromatic erythrocytes (MNPCE) compared to the negative control group (p < 0.05). Concerning antimutagenicity, the doses of 10 and 30 mg/kg co-administered with mitomycin C showed significant decrease in MNPCE frequency compared to the positive control group (p < 0.05). However, no significant reduction in MNPCE frequency (p > 0.05) was detected at the doses of 50 and 100 mg/kg. Under our experimental conditions, the results obtained indicate strong mutagenic and cytotoxic activity of S. umbellatum latex except the dose of 10 mg/kg and moderate antimutagenic effect at lower doses.

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