scholarly journals Multiple dengue virus serotypes resistant transgenic Aedes aegypti fitness evaluated under laboratory conditions

RNA Biology ◽  
2020 ◽  
Vol 17 (7) ◽  
pp. 918-929 ◽  
Author(s):  
Hewawasam Patuwatha Badathuruge Kal Ramyasoma ◽  
Ranil Samantha Dassanayake ◽  
Menaka Hapugoda ◽  
Margareth L. Capurro ◽  
Yasanthi Illika Nilmini Silva Gunawardene
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Laboratory Conditions ◽  
Dengue Virus Serotypes

scholarly journals Cocirculation of two dengue virus serotypes in individual and pooled samples of Aedes aegypti and Aedes albopictus larvae

2011 ◽  
Vol 44 (1) ◽  
pp. 103-105 ◽  
Author(s):  
José Eduardo Marques Pessanha ◽  
Waleska Teixeira Caiaffa ◽  
Alzira Batista Cecilio ◽  
Felipe Campos de Melo Iani ◽  
Simone Costa Araujo ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Clinical Presentation ◽  
Rt Pcr ◽  
Virus Rna ◽  
Dengue Virus Serotypes ◽  
Belo Horizonte ◽  
The City ◽  
Pooled Samples ◽  
Virological Surveillance

INTRODUCTION: To detect dengue virus, eggs of Aedes sp were collected in the city of Belo Horizonte, Brazil, in 2007. METHODS: Egg samples were subsequently hatched and the larvae were tested for the presence of dengue virus RNA by RT-PCR. RESULTS: Among the Aedes aegypti larvae samples, 163 (37.4%) out of 435 were positive, including 32 (10.9%) of 293 individual larvae samples concomitantly positive for two serotypes. CONCLUSIONS: Virological surveillance detecting coinfected vectors in the field could represent an important strategy for understanding the numerous factors involved in the transmission and clinical presentation of dengue.

scholarly journals Incrimination of Aedes aegypti and Aedes albopictus as vectors of dengue virus serotypes 1, 2 and 3 from four states of Northeast India

2020 ◽  
Vol 2 (4) ◽  
Author(s):  
Sumi Chetry ◽  
Saurav Jyoti Patgiri ◽  
Dibya Ranjan Bhattacharyya ◽  
Prafulla Dutta ◽  
N. Pradeep Kumar
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Aedes Albopictus ◽  
Northeast India ◽  
Dengue Virus Serotypes

scholarly journals Vector competence of Aedes aegypti from New Caledonia for the four recent circulating dengue virus serotypes

2020 ◽  
Vol 14 (5) ◽  
pp. e0008303
Author(s):  
Olivia O’Connor ◽  
Elodie Calvez ◽  
Catherine Inizan ◽  
Nicolas Pocquet ◽  
Vincent Richard ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
New Caledonia ◽  
Vector Competence ◽  
Dengue Virus Serotypes

First Evidence of Horizontal Transmission by Fecal Shedding of Dengue Virus 4 Among Aedes aegypti Larvae (Diptera: Culicidae) Under Laboratory Conditions

2022 ◽  
Author(s):  
Edith H. Torres-Montoya ◽  
Leonardo Ulloa-Urquidy ◽  
José I. Torres-Avendaño ◽  
José Marcial Zazueta-Moreno ◽  
Annete I. Apodaca-Medina ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Horizontal Transmission ◽  
Fecal Shedding ◽  
Laboratory Conditions

Detection of dengue virus in Aedes mosquitoes in Delhi, India

ENTOMON ◽  
2019 ◽  
Vol 44 (3) ◽  
pp. 213-218
Author(s):  
Suresh Chand Kaushik ◽  
Sukhvir Singh ◽  
Purnima Srivastava ◽  
R. Rajendran
Keyword(s):  
Early Intervention ◽  
Early Detection ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Infection Rate ◽  
Transmission Season ◽  
Aedes Mosquitoes ◽  
Alternative Approach ◽  
Human Sera ◽  
Minimum Infection Rate

Detection of viruses in human sera particularly in endemic areas is cumbersome and laborious. Therefore, an alternative approach, Immuno-fluorescence assay (IFA) was performed to determine dengue virus (DENV) positivity in mosquitoes. A total of 1055 adult Aedes aegypti female mosquitoes were tested for IFA test against DENV. Minimum infection rate (MIR) for DENV was found higher during August to November 2016 ranging from 10.75 to 20.83. The average yearly MIR was about 6.64. Higher MIR for Ae. aegypti was found in Sarfabad, Noida (12.71) and Khoda Colony, Ghaziabad (11.90). Minimum MIR (4.67) was observed in Sanjay colony (Faridabad). The main contribution of this study resides in the development of a more suitable monitoring system for early detection of viral circulation and to prioritize early intervention in the non-transmission season.

Difusi dan Pola Spasial Sebaran Penyakit Demam Berdarah Dengue (DBD) Di Kota Bandar Lampung

2018 ◽  
Vol 1 (1) ◽  
pp. 87-95
Author(s):  
Nurul Qamila ◽  
Agel Vidian Krama
Keyword(s):  
Public Health ◽  
Spatial Analysis ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Spatial Pattern ◽  
Dengue Hemorrhagic Fever ◽  
Health Problem ◽  
Public Health Problem ◽  
Hemorrhagic Fever ◽  
Descriptive Method

Dengue hemorrhagic fever (DHF) is a contagious disease caused by the dengue virus and is transmitted by the mosquito Aedes aegypti (Aa.aegypti). The population is still a public health problem that increases the number of sufferers and also widespread, with population and education. This study aims to reveal the spatial pattern and distribution of Dengue Hemorrhagic Fever (DHF) with the spatial pattern and the spread of Dengue Hemorrhagic Fever (DHF) can result in different locations of these allegations. From the map that can be used for the prevention of Dengue Hemorrhagic Fever (DBD) in Bandar Lampung City. This study aims to reveal the spatial pattern and distribution of Dengue Hemorrhagic Fever (DHF) with the descriptive method and spatial pattern of Dengue Hemorrhagic Fever (DHF) can result in different locations of these allegations. From the map that can be used for the prevention of Dengue Hemorrhagic Fever (DBD) in Bandar Lampung City. Keywords: DHF, Spatial Analysis

scholarly journals The Lethal(2)-Essential-for-Life [L(2)EFL] Gene Family Modulates Dengue Virus Infection in Aedes aegypti

2020 ◽  
Vol 21 (20) ◽  
pp. 7520
Author(s):  
Lucky R. Runtuwene ◽  
Shuichi Kawashima ◽  
Victor D. Pijoh ◽  
Josef S. B. Tuda ◽  
Kyoko Hayashida ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Cell Line ◽  
Dengue Infection ◽  
Fruit Fly ◽  
Initiation Factor ◽  
Poly I:C ◽  
Gene Products ◽  
Vector Mosquito

Efforts to determine the mosquito genes that affect dengue virus replication have identified a number of candidates that positively or negatively modify amplification in the invertebrate host. We used deep sequencing to compare the differential transcript abundances in Aedes aegypti 14 days post dengue infection to those of uninfected A. aegypti. The gene lethal(2)-essential-for-life [l(2)efl], which encodes a member of the heat shock 20 protein (HSP20) family, was upregulated following dengue virus type 2 (DENV-2) infection in vivo. The transcripts of this gene did not exhibit differential accumulation in mosquitoes exposed to insecticides or pollutants. The induction and overexpression of l(2)efl gene products using poly(I:C) resulted in decreased DENV-2 replication in the cell line. In contrast, the RNAi-mediated suppression of l(2)efl gene products resulted in enhanced DENV-2 replication, but this enhancement occurred only if multiple l(2)efl genes were suppressed. l(2)efl homologs induce the phosphorylation of eukaryotic initiation factor 2α (eIF2α) in the fruit fly Drosophila melanogaster, and we confirmed this finding in the cell line. However, the mechanism by which l(2)efl phosphorylates eIF2α remains unclear. We conclude that l(2)efl encodes a potential anti-dengue protein in the vector mosquito.

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