Faculty Opinions recommendation of Field- and clinically derived estimates of Wolbachia-mediated blocking of dengue virus transmission potential in Aedes aegypti mosquitoes.

Author(s):  
Jean-Luc Imler ◽  
Nelson Martins
2017 ◽  
Vol 115 (2) ◽  
pp. 361-366 ◽  
Author(s):  
Lauren B. Carrington ◽  
Bich Chau Nguyen Tran ◽  
Nhat Thanh Hoang Le ◽  
Tai Thi Hue Luong ◽  
Truong Thanh Nguyen ◽  
...  

The wMel strain of Wolbachia can reduce the permissiveness of Aedes aegypti mosquitoes to disseminated arboviral infections. Here, we report that wMel-infected Ae. aegypti (Ho Chi Minh City background), when directly blood-fed on 141 viremic dengue patients, have lower dengue virus (DENV) transmission potential and have a longer extrinsic incubation period than their wild-type counterparts. The wMel-infected mosquitoes that are field-reared have even greater relative resistance to DENV infection when fed on patient-derived viremic blood meals. This is explained by an increased susceptibility of field-reared wild-type mosquitoes to infection than laboratory-reared counterparts. Collectively, these field- and clinically relevant findings support the continued careful field-testing of wMel introgression for the biocontrol of Ae. aegypti-born arboviruses.


2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Daniela da Silva Goncalves ◽  
Kien Duong Thi Hue ◽  
Vi Tran Thuy ◽  
Nhu Vu Tuyet ◽  
Giang Nguyen Thi ◽  
...  

Abstract Background Dengue viruses (DENV) can be transmitted from an adult female Aedes aegypti mosquito through the germ line to the progeny; however, there is uncertainty if this occurs at a frequency that is epidemiologically significant. We measured vertical transmission of DENV from field-reared Ae. aegypti to their F1 progeny after feeding upon blood from dengue patients. We also examined the transmission potential of F1 females. Methods We examined the frequency of vertical transmission in field-reared mosquitoes, who fed upon blood from acutely viremic dengue patients, and the capacity for vertically infected females to subsequently transmit virus horizontally, in two sets of experiments: (i) compared vertical transmission frequency of field-reared Ae. aegypti and Ae. albopictus, in individual progeny; and (ii) in pooled progeny derived from field- and laboratory-reared Ae. aegypti. Results Of 41 DENV-infected and isofemaled females who laid eggs, only a single female (2.43%) transmitted virus to one of the F1 progeny, but this F1 female did not have detectable virus in the saliva when 14 days-old. We complemented this initial study by testing for vertical transmission in another 460 field-reared females and > 900 laboratory-reared counterparts but failed to provide any further evidence of vertical virus transmission. Conclusions In summary, these results using field-reared mosquitoes and viremic blood from dengue cases suggest that vertical transmission is uncommon. Field-based studies that build on these observations are needed to better define the contribution of vertical DENV transmission to dengue epidemiology.


2020 ◽  
Vol 33 (1) ◽  
pp. 38-47
Author(s):  
Manuel Castillo-Méndez ◽  
Verónica Valverde-Garduño

2016 ◽  
Vol 37 (4) ◽  
pp. 167
Author(s):  
Andrew F van den Hurk

Dengue virus outbreaks involving 100s of cases periodically occur in north Queensland, the area of Australia where the primary mosquito vector, Aedes aegypti, occurs. This article summarises the ecology, history, current situation and control of dengue virus transmission in Australia and examines the threat posed by newly emergent arboviruses, such as Zika and chikungunya viruses.


2020 ◽  
Vol 21 (18) ◽  
pp. 6609
Author(s):  
Alexander S. Gold ◽  
Fabiana Feitosa-Suntheimer ◽  
Ricardo V. Araujo ◽  
Ryan M. Hekman ◽  
Sultan Asad ◽  
...  

Dengue is the most burdensome vector-borne viral disease in the world. Dengue virus (DENV), the etiological cause of dengue, is transmitted primarily by the Aedes aegypti mosquito. Like any arbovirus, the transmission cycle of dengue involves the complex interactions of a multitude of human and mosquito factors. One point during this transmission cycle that is rich in these interactions is the biting event by the mosquito, upon which its saliva is injected into the host. A number of components in mosquito saliva have been shown to play a pivotal role in the transmission of dengue, however one such component that is not as well characterized is extracellular vesicles. Here, using high-performance liquid chromatography in tandem with mass spectrometry, we show that dengue infection altered the protein cargo of Aedes aegypti extracellular vesicles, resulting in the packaging of proteins with infection-enhancing ability. Our results support the presence of an infection-dependent pro-viral protein packaging strategy that uses the differential packaging of pro-viral proteins in extracellular vesicles of Ae. aegypti saliva to promote transmission. These studies represent the first investigation into the function of Ae. aegypti extracellular vesicle cargo during dengue infection.


2020 ◽  
Vol 19 (2) ◽  
pp. 111-118
Author(s):  
Esy Maryanti ◽  
Ismawati Ismawati ◽  
Unique Prissilia ◽  
Ardini Yovy Puteri

Latar belakang: Demam berdarah dengue (DBD) merupakan penyakit yang masih menjadi masalah kesehatan di Indonesia yang disebabkan oleh virus dengue dan ditularkan melalui nyamuk Aedes aegypti. Kejadian DBD selalu ada setiap tahun di Pekanbaru dari tahun 2014-2016 terjadi peningkatan kasus, 2017-2018 kasus DBD mulai menurun tetapi diawal tahun 2019 kasus DBD kembali terjadi peningkatan dan sudah ada kematian sehingga memerlukan perhatian yang lebih serius untuk meningkatkan kewaspadaan terhadap penyakit tersebut. Tujuan penelitian ini adalah untuk menganalisis potensi transmisi DBD berdasarkan indeks entomologi dan maya indeks di tiga kelurahan Kecamatan Sukajadi yang merupakan daerah endemis DBD. Metode: Penelitian survei larva Aedes aegypti dengan pendekatan cross sectional yang dilakukan pada bulan Juli 2019 di tiga kelurahan yaitu Kelurahan Kampung Tengah, Kampung Melayu, Kedungsari Kecamatan Sukajadi. Identifikasi larva menggunakan metode single larva method. Hasil: Sebanyak 181 rumah yang disurvey, didapatkan 822 kontainer yang terdiri dari 683 controllable sites dan 139 disposable sites. Angka bebas jentik sebesar 89,5%, container index 3,4%, house index 10,5% dan Breteau index 21%. Status Maya indeks (MI) yang diukur berdasarkan breeding risk index dan hygiene risk index didapatkan sebanyak 55,80% rumah termasuk dalam status sedang dan 15,47% termasuk ke dalam status MI tinggi. Sebaran keberadaan larva Aedes aegypti berdasarkan letak geografis cukup merata di Kelurahan Kampung Melayu dan Kampung Tengah.Simpulan: Berdasarkan hasil indeks entomologi tersebut didapatkan density figure dalam kategori sedang yang artinya wilayah ini mempunyai potensi transmisi sedang untuk kejadian penyakit DBD. ABSTRACT  Title : Transmission Potential of Dengue hemorrhagic fever based on entomology index and maya index in three sub-districts, Sukajadi District, Pekanbaru CityBackground: Dengue hemorrhagic fever (DHF) is a disease still a health problem in Indonesia caused by the dengue virus that is transmitted through Aedes aegypti. The incidence of DHF is always there every year in Pekanbaru form 2014-2016 there was an increase in cases, from 2017-2018 dengue cases began to decline, but in early 2019 dengue cases increased again and there have been deaths so it requires more serious attention.. The objectives of this study was to analyze the potential transmission of DHF based on the entomology index and maya index in three sub-districts of Sukajadi district.Method: The research was a survey of larva Ae.aegypti in July 2019 from 181 houses in the three village of Sukajadi District. Larvae identification using the single larvae method.Result: A total of 181 houses surveyed obtained 822 containers consisting of 683 controllable sites and 139 disposable sites. The larvae free rates is 89.5%, container index 3.4%, house index 10,5% and Breteau index 21%. Maya index is measures based on breeding risk index and hygiene risk index shows that there are 55.8% of houses in the area are classified as moderate and 15.47% are included in high. The distribution of the existence of Ae.aegypti larvae based on the geographical location is quite evenly distributed in Kampung Melayu and Kampung Tengah.Conclusion: It is found that density figure is in the medium, which means that region has moderate transmission potential for the incidence of DHF


2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Grace Trovancia ◽  
Angle Sorisi ◽  
Josef S.B. Tuda

Abstract: Dengue hemorrhagic fever is an acute disease with clinical manifestations of hemorrhage caused by dengue virus infection. Manado is endemic dengue. Dengue virus has the ability to maintain its existence in nature through horizontal and vertical transmission. There are several ways to detect the dengue virus by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and immunohistochemistry Streptavidin Biotin Peroxidase Complex (ISBPC). This research aims to determine the wild Aedes aegypti population in Manado and to detect dengue virus in wild mosquito Aedes aegypti by ISBPC methods. This was a descriptive survey study with a cross sectional design to describe the transmission of dengue virus in wild mosquito Aedes aegypti in the city of Manado. The results showed that there were 5 wild Aedes aegypti mosquitoes positive for dengue virus, and 36 wild Aedes aegypti mosquitoes negative containing dengue virus. Conclusion: Of the 41 samples immunohistochemistry tested, 5 samples showed dengue virus transmission in wild mosquito Aedes aegypti in Manado which is a positive possibility of horizontal transmission.Keywords: detection of dengue virus, transmission, wild Aedes aegypti, Manado. Abstrak: Demam berdarah dengue adalah suatu penyakit akut dengan manifestasi klinis perdarahan yang disebabkan oleh infeksi virus dengue. Manado merupakan daerah endemis demam berdarah. Virus dengue memiliki kemampuan untuk mempertahankan keberadaannya di alam melalui transmisi horizontal dan vertikal. Ada beberapa cara untuk mendeteksi virus dengue yaitu Reverse Transcription-Polymerase Chain Reaction (RT-PCR) dan imunohistokimia Streptavidin Biotin Peroxidase Complex (SBPC). Penelitian ini bertujuan untuk mengetahui populasi nyamuk wild Aedes aegypti di Kota Manado dan mendeteksi virus dengue pada nyamuk wild Aedes aegypti dewasa menggunakan metode imunohistokimia streptavidin biotin peroxidase complex (ISBPC). Jenis penelitian ialah survei deskriptif dengan desain potong lintang untuk mengetahui gambaran transmisi virus dengue pada nyamuk wild Aedes aegypti betina di Kota Manado. Hasil pene;itian mendapatkan 5 nyamuk wild Aedes aegypti positif mengandung virus dengue, dan 36 nyamuk wild Aedes aegypti negatif mengandung virus dengue. Simpulan: Berdasarkan hasil penelitian dapat disimpulkan bahwa dari 41 sampel yang telah diuji imunohistokimia, 5 sampel gambaran transmisi virus dengue pada nyamuk wild Aedes aegypti betina di Kota Manado yang kemungkinan transmisi horizontal adalah positif. Kata kunci: deteksi virus dengue, transmisi, wild Aedes aegypti, Manado.


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