Defense priming by non-jasmonate producing fatty acids in maize (Zea mays)

Objective: The aim of this work was to determine the fatty acids content in corn seeds oil (Zea mays) sample cultivated in Ecuador.Methods: Corn oil was obtained from corn oil seeds using the cold pressing method. Methyl esters fatty acids analysis were carried out using the gas chromatography (GC) method with a mass selective detector and using the database library NIST 14.L to identify the compounds present in the corn seed oil.Results: Methyl esters fatty acids were identified from corn (Z. mays) seeds using the GC mass spectrometer (GC-MS) analytical method. Fatty acids were analyzed as methyl esters on a capillary column DB-WAX 122-7062 with a good separation of palmitic acid, stearic acid, oleic acid, elaidic acid, linoleic acid, arachidic acid, and linolenic acid. The structure of methyl esters fatty acids was determined using the GS-MS method. Corn oil has a high content of linoleic acid (omega 6) with a value of 52.68% of the total content of fatty acids in corn oil and 29.70% of oleic acid (omega 9) of the total content of fatty acids in corn oil. The sample presented a value of 12.57% of palmitic acid.Conclusions: Corn oil shows a good content of fatty acids omega 6 and 9. The higher value was of omega 6 with 52.68% content. Corn oil has a good proportion of polyunsaturated of lipids (53.80%) and 14.86% of saturated lipids.

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Effect of Different Cytoplasms on Oil, Fatty Acids, Plant Height, and Ear Height in Maize ( Zea mays L.) 1

Crop Science ◽

10.2135/cropsci1970.0011183x001000010015x ◽

1970 ◽

Vol 10 (1) ◽

pp. 39-41 ◽

Cited By ~ 19

Author(s):

D. L. Garwood ◽

Evelyn J. Weber ◽

R. J. Lambert ◽

D. E. Alexander

Keyword(s):

Fatty Acids ◽

Zea Mays ◽

Plant Height ◽

Zea Mays L ◽

Ear Height

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Reversal of EPTC Induced Fatty Acid Synthesis Inhibition

Weed Science ◽

10.1017/s0043174500052565 ◽

1975 ◽

Vol 23 (2) ◽

pp. 90-92 ◽

Cited By ~ 25

Author(s):

R. E. Wilkinson ◽

A. E. Smith

Keyword(s):

Fatty Acids ◽

Zea Mays ◽

Fatty Acid ◽

Fatty Acid Synthesis ◽

Spinacia Oleracea ◽

Acid Synthesis ◽

Acetate Incorporation ◽

Naphthalic Anhydride ◽

Synthesis Inhibition ◽

Fatty Acid Synthesis Inhibition

EPTC (S-ethyl dipropylthiocarbamate), 1,8-naphthalic anhydride (NA),N,N-dialryl-2,2-dichloroacetamide (R-25788),CDAA(N,N-diallyl-2-chloroacetamide) and CDEC (2-chloroallyl diethyldithiocarbamate) at concentrations of 10-5M inhibited the incorporation of acetate-1-14C (Ac∗) into fatty acids of isolated spinach (Spinacia oleraceaL.) chloroplasts. Lower concentrations of these chemicals did not affect acetate incorporation into fatty acids. However, the inhibition by EPTC at 10-5M was counteracted by NA and R-25788 [agriculturally important antidotes for EPTC injury to corn (Zea maysL.)] at 10-7M.

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Gene Transformation by Chitosan Nanoparticle to Enhance Fatty Acid Production in Zea mays (L.)

ROMANIAN BIOTECHNOLOGICAL LETTERS ◽

10.25083/rbl/26.5/2971.2978 ◽

2021 ◽

Vol 26 (5) ◽

pp. 2971-2978

Author(s):

EMAN TAWFIK HUSSIEN ◽

◽

MOHAMMED IBRAHIM DAHAB ◽

KAREEM MOHAMMED ABD-ELATTY ◽

ISLAM HAMDY EL-SHENAWY ◽

...

Keyword(s):

Fatty Acids ◽

Zea Mays ◽

Fatty Acid ◽

Acid Production ◽

Chitosan Nanoparticles ◽

Gene Transformation ◽

Gas Chromatography Mass Spectrometry ◽

Acetyl Coa ◽

Fatty Acid Production ◽

Transgenic Lines

Zea mays is an important crop and an essential source of fatty acids. Hence, increasing and adding new fatty acids led to the enhancement of these properties. Transformation of external Acetyl-CoA gene (Aco) can enhance fatty acid components, as ACo is expressed into Acetyl-CoA carboxylase (ACCase) enzyme, which is the first essential step in the fatty acid production process. Chitosan nanoparticles are safe and fast polymer nanoparticles that are applied for gene transformation. Conventional PCR was performed for the detection of the ACo gene in both transgenic and nontransgenic maize lines. The results confirm the presence of the gene in the transgenic lines and absence in non-transgenic lines. The Gas chromatography-mass spectrometry (GC-MS) analysis for fatty acid contents in transgenic and non-transgenic maize lines showed an increase in fatty acid contents in transgenic lines compared to non-transgenic ones. Besides, the transgenic maize’s lines produced extra new fatty acids not found in non-transgenic ones.

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