scholarly journals Ionic control of the size of the vesicle matrix of beige mouse mast cells.

1991 ◽  
Vol 98 (4) ◽  
pp. 771-790 ◽  
Author(s):  
M J Curran ◽  
M S Brodwick

Isolated matrices of the giant secretory vesicles of mast cells of the beige mouse were reliably produced by the osmotic lysis of isolated vesicles. These matrices maintained their form, and their sizes were easily measured using Nomarski optics. The size of the matrix depended on the ionic composition of the bathing solution. The physiologically relevant ions, histamine and serotonin, contracted the matrix. Multivalent cations condensed the matrix relative to univalents. Ag+, acid pH (below 5), and basic pH (above 9) expanded the matrix. In the presence of 10 mM histamine, lowering the pH from 9 to 5 contracted the matrix more than can be attributed to the pH-dependent matrix contraction in zero histamine. The nontitratable organic cation, dimethonium, contracts the matrix with little effect of pH in the range of 5-9. These results suggest that histamine acts as a matrix contractor in the divalent form. The dose-response (contraction) relation for histamine was gradual from micromolar to 316 mM (millimolar) histamine. Experiments with mixtures of histamine and sodium show antagonistic effects on the matrix but are inconsistent with either a model where ions compete for identical sites or a parallel model where ions interact with separate independent sites. In vigorous histamine washoff experiments, the half time for vesicle expansion in 10(-4) M pH buffer was approximately 4 s; in isotonic NaCl solution, it was 0.5 s. When 1 M histamine was presented to closely apposed matrices, fusion resulted. The matrix material returned to its initial shape after being mechanically deformed with a glass probe. These results suggest that the matrix size is controlled by its ion exchange properties. The matrix expansion can quantitatively account for the vesicular size increase observed upon exocytosis (as a postfusional event) and the osmotic nonideality of intact vesicles. The mechanical expansion is probably significant in the widening of the exocytotic pore and the dispersal of the vesicular contents.

Author(s):  
C.T. Hu ◽  
C.W. Allen

One important problem in determination of precipitate particle size is the effect of preferential thinning during TEM specimen preparation. Figure 1a schematically represents the original polydispersed Ni3Al precipitates in the Ni rich matrix. The three possible type surface profiles of TEM specimens, which result after electrolytic thinning process are illustrated in Figure 1b. c. & d. These various surface profiles could be produced by using different polishing electrolytes and conditions (i.e. temperature and electric current). The matrix-preferential-etching process causes the matrix material to be attacked much more rapidly than the second phase particles. Figure 1b indicated the result. The nonpreferential and precipitate-preferential-etching results are shown in Figures 1c and 1d respectively.


Author(s):  
D. E. Luzzi ◽  
L. D. Marks ◽  
M. I. Buckett

As the HREM becomes increasingly used for the study of dynamic localized phenomena, the development of techniques to recover the desired information from a real image is important. Often, the important features are not strongly scattering in comparison to the matrix material in addition to being masked by statistical and amorphous noise. The desired information will usually involve the accurate knowledge of the position and intensity of the contrast. In order to decipher the desired information from a complex image, cross-correlation (xcf) techniques can be utilized. Unlike other image processing methods which rely on data massaging (e.g. high/low pass filtering or Fourier filtering), the cross-correlation method is a rigorous data reduction technique with no a priori assumptions.We have examined basic cross-correlation procedures using images of discrete gaussian peaks and have developed an iterative procedure to greatly enhance the capabilities of these techniques when the contrast from the peaks overlap.


2020 ◽  
Vol 38 (7A) ◽  
pp. 960-966
Author(s):  
Aseel M. Abdullah ◽  
Hussein Jaber ◽  
Hanaa A. Al-Kaisy

In the present study, the impact strength, flexural modulus, and wear rate of poly methyl methacrylate (PMMA) with eggshell powder (ESP) composites have been investigated. The PMMA used as a matrix material reinforced with ESP at two different states (including untreated eggshell powder (UTESP) and treated eggshell powder (TESP)). Both UTESP and TESP were mixed with PMMA at different weight fractions ranged from (1-5) wt.%. The results revealed that the mechanical properties of the PMMA/ESP composites were enhanced steadily with increasing eggshell contents. The samples with 5 wt.% of UTESP and TESP additions give the maximum values of impact strength, about twice the value of the pure PMMA sample. The calcination process of eggshells powders gives better properties of the PMMA samples compared with the UTESP at the same weight fraction due to improvements in the interface bond between the matrix and particles. The wear characteristics of the PMMA composites decrease by about 57% with increases the weight fraction of TESP up to 5 wt.%. The flexural modulus values are slightly enhanced by increasing of the ESP contents in the PMMA composites.


Polymers ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1235
Author(s):  
Bidita Salahuddin ◽  
Rahim Mutlu ◽  
Tajwar A. Baigh ◽  
Mohammed N. Alghamdi ◽  
Shazed Aziz

Passive vibration control using polymer composites has been extensively investigated by the engineering community. In this paper, a new kind of vibration dampening polymer composite was developed where oriented nylon 6 fibres were used as the reinforcement, and 3D printed unoriented nylon 6 was used as the matrix material. The shape of the reinforcing fibres was modified to a coiled structure which transformed the fibres into a smart thermoresponsive actuator. This novel self-reinforced composite was of high mechanical robustness and its efficacy was demonstrated as an active dampening system for oscillatory vibration of a heated vibrating system. The blocking force generated within the reinforcing coiled actuator was responsible for dissipating vibration energy and increase the magnitude of the damping factor compared to samples made of non-reinforced nylon 6. Further study shows that the appropriate annealing of coiled actuators provides an enhanced dampening capability to the composite structure. The extent of crystallinity of the reinforcing actuators is found to directly influence the vibration dampening capacity.


2019 ◽  
Vol 809 ◽  
pp. 480-486
Author(s):  
Rohit George Sebastian ◽  
Christof Obertscheider ◽  
Ewald Fauster ◽  
Ralf Schledjewski

The growing use of composite materials has generated interest in improving and optimising composite manufacturing processes such as Liquid Composite Moulding (LCM). In LCM, dry preforms are placed in a mould and impregnated with the matrix material. The efficiency of filling the moulds can be improved by using Computational Fluid Dynamics (CFD) filling simulations during the design of the mould. As part of an on-going effort to develop a CFD tool for the simulation of LCM processes, a volume averaged energy balance equation has been derived and implemented in a custom OpenFOAM solver. The energy balance is implemented in a custom OpenFOAM solver with and without the pressure terms for comparison with results from RTM experiments. It is found that the pressure terms do not significantly influence the results for LCM processes.


2013 ◽  
Vol 535-536 ◽  
pp. 164-167
Author(s):  
Jonghun Yoon ◽  
Oana Cazacu ◽  
Jung Hwan Lee

In spite of this progress in predicting ductile failure, the development of macroscopic yield criteria for describing damage evolution in HCP (hexagonal close-packed) materials remains a challenge. HCP materials display strength differential effects (i.e., different behavior in tension versus compression) in the plastic response due to twinning. Cazacu and Stewart [1] developed an analytic yield criterion for a porous material containing randomly distributed spherical voids in an isotropic, incompressible matrix that displays tension-compression asymmetry. The matrix material was taken to obey the isotropic form of the Cazacu et al. [2] yield criterion, which captures the tension-compression asymmetry of the matrix material. In this paper, finite element calculations of a round tensile bar are conducted with the material behavior described by the Cazacu and Stewart [1] yield criterion. The goal of these calculations is to investigate the effect of the tension-compression asymmetry on the necking induced by void evolution and propagation.


2021 ◽  
pp. 002199832110112
Author(s):  
Qing Yang Steve Wu ◽  
Nan Zhang ◽  
Weng Heng Liew ◽  
Vincent Lim ◽  
Xiping Ni ◽  
...  

Propagation of ultrasonic wave in Carbon Fiber Reinforced Polymer (CFRP) is greatly influenced by the material’s matrix, resins and fiber volume ratio. Laser ultrasonic broadband spectral technique has been demonstrated for porosity and fiber volume ratio extraction on unidirection aligned CFRP laminates. Porosity in the matrix materials can be calculated by longitudinal wave attenuation and accurate fiber volume ratio can be derived by combined velocity through the high strength carbon fiber and the matrix material with further consideration of porosity effects. The results have been benchmarked by pulse-echo ultrasonic tests, gas pycnometer and thermal gravimetric analysis (TGA). The potentials and advantages of the laser ultrasonic technique as a non-destructive evaluation method for CFRP carbon fiber volume fraction evaluation were demonstrated.


2015 ◽  
Vol 22 (5) ◽  
pp. 969-987 ◽  
Author(s):  
Ivan Giorgio ◽  
Ugo Andreaus ◽  
Tomasz Lekszycki ◽  
Alessandro Della Corte

Since internal architecture greatly influences crucial factors for tissue regeneration, such as nutrient diffusion, cell adhesion and matrix deposition, scaffolds have to be carefully designed, keeping in mind case-specific mechanical, mass transport and biological requirements. However, customizing scaffold architecture to better suit conflicting requirements, such as biological and mechanical ones, remains a challenging issue. Recent advances in printing technologies, together with the synthesis of novel composite biomaterials, have enabled the fabrication of various scaffolds with defined shape and controlled in vitro behavior. Thus, the influence of different geometries of the assemblage of the matrix and scaffold on the remodeling processes of living bone and artificial material should be investigated. To this end, two implant shapes are considered in this paper, namely a circular inclusion and a rectangular groove of different aspect ratios. A model of a mixture of bone tissue and bioresorbable material with voids was used to numerically analyze the physiological balance between the processes of bone growth and resorption and artificial material resorption in a plate-like sample. The adopted model was derived from a theory for the behavior of porous solids in which the matrix material is elastic and the interstices are void of material.


2021 ◽  
Vol 39 (2A) ◽  
pp. 196-205
Author(s):  
Zainab M. Abdul Monem ◽  
Jawad K. Oleiwi ◽  
Qahtan A. Hamad

In the current Research , the heat cured   matrix material powder of PMMA was reinforced with peanut and walnut shells (natural powders) which are chemically treated with 5% (w/v) (NaOH) to improve the matrix bonding (PMMA) before being used as a reinforcing powder and adding to exactly similar averages particle sizes ≤ (53µm), with different weight fractions of (4, 8, and 12 wt.%). The ASTM D638 is used for composite specimens of the tensile test. The results indicated that the Elastic modulus values reached its maximum value at (8 wt.%.) when reinforced with peanut shells particles (1.053Gpa) , while ,the values of tensile strength, elongation percentage at break, decrease as the weight fraction of peanut and walnut shells powder increase and the lowest values is obtained by reinforcing with peanut shells particles to reach their minimum values at (12 wt.%.) where the lowest values of them are (29 MPa, 2.758% ) respectively. The fracture surface morphology of pure PMMA seemed to be homogenous morphology in (SEM) test, whereas the fracture surface morphology of PMMA composite reinforced by (peanut and walnut shells) powders and shows a roughness fracture surface morphology this refer to semi ductile to ductile materials.


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