scholarly journals Analytical Study of the Royal-Jelly Effect on Sperms Morphological Characteristics and some Liver Function Parameters and their Correlation with some Antioxidants in Male Albino Rats Treated with Pregabalin Drug

2021 ◽  
Vol 2114 (1) ◽  
pp. 012056
Author(s):  
L. A. Salih ◽  
M.H. Nawar ◽  
S. M. Muuhsen
Keyword(s):  
Natural Products ◽  
Liver Function ◽  
Normal Level ◽  
Royal Jelly ◽  
Morphological Characteristics ◽  
Reproductive Hormones ◽  
Control Group ◽  
Albino Rats ◽  
Antioxidants Enzymes ◽  
Pharmaceutical Industries

Abstract In recent years, the consideration of natural products as anti-inflammatory and antioxidative treatments has more interested worldwide. Moreover, natural products are easily obtained and are relatively safe the Royal jelly (RJ) is one of them. The current study was carried to evaluate the effects of pregabalin (PGB) on physiological activity of sperms, reproductive hormones assay and some biochemical analysis. Forty (40) male albino rats (10-weeks-old) were divided into four groups (10 rats each): G1 (treated with PGB drug, 150 mg/kg B.wt (Lyrica-Pfizer-Pharmaceutical Industries), G2 (treated with RJ 1g/kg), G3 (treated with PGB drug and RJ together), and G4 control treated with normal saline only. all groups were treated orally via stomach tube for 60 consecutive days.. PGB caused a significant reduction in sperm count, sperm viability, motility and sperm abnormality percent, serum FSH, LH and testosterone levels, the co-administration of RJ caused normal level of the previous tests near the control group level. Results of the lipid profile and some the present results of the liver function parameters showed increased concentrations in PGB-treated group, but after treated with RJ the levels were recovered to normal level. In the studied antioxidants markers, the results appeared high level in malondialdehyde (MDA) and low level in the antioxidants enzymes; superoxide dismutase (SOD) and catalase (CAT) the RJ was appeared direct effect on MDA and showed decreased concentration of MDA and increased levels in the studied antioxidants enzymes of the PGB group which treated with RJ.

scholarly journals Evaluation of Anti-oxidant Enzymes, Lipid Peroxidation, Lipid Profile and Liver Function in Albino Rats Orally Administered Tartrazine

2020 ◽  
pp. 19-29
Author(s):  
Uyota Anthony Adele ◽  
Geraldine Iroh ◽  
Ojoye Ngoye Briggs ◽  
Helen Anthony Waribo ◽  
Ibioku Elekima
Keyword(s):  
Lipid Peroxidation ◽  
Liver Function ◽  
Lipid Profile ◽  
Whole Blood ◽  
Test Group ◽  
Control Group ◽  
Albino Rats ◽  
Central Vein ◽  
Significant Difference ◽  
Anti Oxidant

Aim: To evaluate the anti-oxidant enzymes, lipid peroxidation, lipid profile and liver function in albino rats orally administered tartrazine. Study Design: A total number of 63 female albino rats weighing approximately 0.2 kg were used for this study. The study was divided into two phases, phase 1 which lasted for the first 30 days, comprised of 35 rats, 20 rats were used as test group while 15 rats served as the control group. Phase 2 of the study was for 60 days and 28 rats were used with 16 as test group and 12 as the control. The test groups were orally administered with 7.5 mg/kg of tartrazine (ADI) daily over the specified periods while the control groups were not treated with tartrazine but given only food and water. Place and Duration of Study: The study was carried out in the Department of Medical Laboratory Science, Rivers State University, Port Harcourt, Nigeria within a period of 12 months (Feb., 2019 – Jan., 2020). Methodology: At the end of the study, 5 mls of whole blood specimens was collected by means of cardiac puncture into plain bottles. To obtain the serum, the whole blood samples were allowed to clot and later dislodged and spun at 3500 rpm for 10 minutes. The collected serum specimens were used to analyze SOD, MDA, GPX, ALT, GGT, ALP, TG, TCHOL, and HDL-C, while LDL-C was calculated using Friedwald equation. Results: The chronic treatment of rats with tartrazine azo food dye at the ADI dose caused an increase in MDA levels after 30 and 60 days test rats compared to the control, while TCHOL and HDL-C showed significant decrease after 30 and 60 days of treatment in the test group compared to the control group. In addition, ALT indicated significant increase in test group after 60 days of treatment compared to control group. ALP, GGT, TG, LDL-C, SOD and GPX showed no significant difference after 30, and 60 days of treatment at ADI doses. Histologic examination of the liver indicated hydropic dilation, degenerating hepatocyes and infiltration of central vein with parenchymal materials alongside kupffer cells. Conclusion: The results from this study revealed that orally administered tartrazine at the recommended ADI dose increased lipid peroxidation as seen in the elevated MDA levels. Hepatic derangements were also seen as revealed by increased ALT and histologic distortions as well fall in TCHOL and HDL-C lipid fractions.

scholarly journals Effects of monosodium-L-glutamate administration on serum levels of reproductive hormones and cholesterol, epididymal sperm reserves and testicular histomorphology of male albino rats

2015 ◽  
Vol 63 (1) ◽  
pp. 125-139 ◽  
Author(s):  
Izuchukwu Ochiogu ◽  
David Ogwu ◽  
Chukwuka Uchendu ◽  
Chidozie Okoye ◽  
John Ihedioha ◽  
...  
Keyword(s):  
Luteinising Hormone ◽  
Serum Levels ◽  
Reproductive Hormones ◽  
Control Group ◽  
Albino Rats ◽  
Low Doses ◽  
Epididymal Sperm ◽  
Lower Serum ◽  
Gonadotrophin Releasing Hormone ◽  
The Mean

This study investigated the effects of administration of monosodium L-glutamate (MSG) on serum gonadotrophin-releasing hormone (GnRH), luteinising hormone (LH), testosterone and total cholesterol (TC), cauda epididymal sperm reserves (CESR) and testicular histomorphology of adult male albino rats. Eighty-four rats, randomly assigned to 7 groups of 12 rats each, were used for the study. Varying low doses (0.25, 0.50 or 1.00 g/kg body weight) of MSG were administered orally or subcutaneously at 48-h intervals for six weeks. Serum GnRH, LH, testosterone and TC, and CESR were evaluated on days 14, 28 and 42 of MSG administration. Testicular histomorphology was evaluated on day 42. The results showed that the mean serum GnRH, LH and testosterone levels, and the CESR of all the treated groups were significantly (P < 0.05) lower than those of the untreated control on days 14, 28 and 42 of MSG administration. The mean serum TC levels of all the treated groups were also significantly (P < 0.05) lower than those of the control group on days 14 and 28. No lesions were observed on sections of the testes. It was concluded that MSG administration for 14, 28 and 42 days led to significantly lower serum levels of GnRH, LH, testosterone and TC, and significantly lower CESR.

Physiological Effects of Using a Complex Preparation Based on Royal Jelly and Propolis in Pulmonary Oedema in Rats

2021 ◽  
pp. 16-24
Author(s):  
Svetlana V. Kopylova ◽  
◽  
Anastasiya A. Anashkina ◽  
Elena I. Erlykina ◽  
Keyword(s):  
Pulmonary Oedema ◽  
Royal Jelly ◽  
Inhalation Therapy ◽  
Control Group ◽  
Albino Rats ◽  
Physiological Effects ◽  
Complex Preparation ◽  
Experimental Group ◽  
Medium Weight ◽  
Intact Rats

This paper aimed to study the physiological effects of inhalation therapy using Apingalin (based on royal jelly and propolis) in experimental pulmonary oedema in rats. The research involved 120 Wistar albino rats that were divided into 3 groups: intact; control (with pulmonary oedema induced by 0.5 mg/kg adrenaline); experimental (with induced pulmonary oedema followed by a 10-minute inhalation of Apingalin during the course of 10 days). In the blood serum of the control group of rats, a decrease in the activity of aspartate transaminase (AST) and alanine transaminase (ALT) was detected; the ratio of albumin to globulin fractions decreased; the concentrations of creatinine, Na+ and K+ ions increased, compared to the intact animals. In the urine of the control group, no statistically significant changes in the concentration of Na+ were found; the concentration of K+ was lower than that in the intact rats. An elevated content of medium-weight molecules on erythrocytes and in the plasma was recorded, as well as decreased concentrations of these molecules in the urine of the control group, compared with the intact animals. After a course of Apingalin inhalations, the rats in the experimental group demonstrated normalized activity of AST and ALT in the blood; the concentrations of creatinine in the blood as well as Na+ and K+ in the blood and urine were restored to the levels of the intact rats. In addition, the experimental group showed a decrease in the content of medium-weight molecules on erythrocytes and in the blood plasma, as well as a significant increase in the concentration of medium-weight molecules in the urine, compared with the control. Thus, a course of Apingalin inhalations in experimental pulmonary oedema in rats reduces the level of endogenous intoxication. This opens up wide prospects for its use in pulmonology.

THE EFFECT OF ELECTROMAGNETIC RADIATION (EMR) FROM LAPTOP ON REPRODUCTIVE HORMONES, SPERM QUALITY AND PROSTATE SPECIFIC ANTIGEN OF MALE ALBINO RATS (RATTUS NORVEGICUS)

2019 ◽  
pp. 43-52
Author(s):  
Stephen S. Aderemi ◽  
Kelechi L. Njoku ◽  
Adeola A. Adesuyi
Keyword(s):  
Electromagnetic Radiation ◽  
Sperm Quality ◽  
Reproductive Hormones ◽  
Quality Analysis ◽  
Control Group ◽  
Albino Rats ◽  
Reproductive Hormone ◽  
Laptop Computer ◽  
Laptop Computers ◽  
Significant Difference

Humans in modern society are exposed to an ever-increasing number of electromagnetic fields (EMFs) generated from the production and supply of electricity, television sets, personal computer (PC), radio communication, and mobile communication, hence, it has become a public health issue. This study was conducted to evaluate the effects of electromagnetic radiation (EMR) emitted from new generation laptop computers on sperm quality and reproductive hormone of male albino rats. Male albino rats (10-12 weeks old) were exposed to RF-EMR from laptop computers which were grouped based on different exposure period (2hours, 4hours, 6hours and 8hours) for 4 weeks. The semen samples were obtained by caudal puncture of the epididymis from each participant for sperm quality analysis and blood collected by cardiac puncture for hormonal analysis using the chemiluminescent microparticles immunoassay method. The analysis of variance was done for the hormonal concentration and sperm quality parameters to check for the significance difference at 5% level of significance. The Dunnett’s multiple comparison test was done to test for significance comparison of radiation exposed groups and control group. Exposure to laptop computer display unit was associated with significant reduction in sperm motility, sperm viability and sperm count (P<0.0001), testosterone level (P<0.001), follicle stimulating hormone level (P<0.01). For the sperm morphology, there was no significant difference in the normal cells for the experimental setup, however, the appearance of abnormal cells in the exposed rats (2 – 8 hrs) were significant (P≤0.001). This study therefore showed that EMR from a charging laptop can significantly affect semen quality, male fertility and rendered male reproductive hormone unstable with no effect on prostrate specific antigens.

scholarly journals A Comparative Evaluation of Selected Medicinal Plants on Male Fertility Indices (Reproductive Hormones and Sperm Profile) of Albino Wistar Rats: An Animal Case Study

2019 ◽  
pp. 1-6
Author(s):  
Oliver E. Ngwu ◽  
J. I. Okoye
Keyword(s):  
Medicinal Plants ◽  
Reproductive Hormones ◽  
Male Rats ◽  
Mucuna Pruriens ◽  
Control Group ◽  
Albino Rats ◽  
High Dose ◽  
Velvet Bean ◽  
Testosterone Concentration ◽  
Group 1

The study aims to investigate the effects of the medicinal plants {seeds} on the  reproductive hormones and sperm profile of male albino rats to ascertain their possible usefulness  as  fertility agent. Walnuts [Tetracarpidium conophorum], Sesame (Sesamine indicum), and Velvet bean (Mucuna pruriens) seeds were obtained and taken to the Department of plant  Science and  Biotechnology, University of  Nigeria, Nsukka (UNN). The seeds were milled into fine powder. One hundred and ten sexually matured aibino rats of about twelve weeks weighing 130-180 g were divided into eleven groups (1-11) using completely randomized design. There were two different control groups and rats in group 1 served as the Control 1 and were fed with normal commercial feed. Rats in group 2 were administered with a drug (Ketoconazole) to induce infertility. The Infertility Induced groups were treated with low dose (groups 3-5), medium dose (groups 6-8) and  high dose (9-11) for the period of nine (9) weeks. At the end and testes and Epididymides were surgically removed and weighed. Blood  sample analysis revealed that the concentration of sex hormones  measured in the male rats fed with medium plants (seeds) showed that the testosterone concentration significanty increased (p<0.05) in animal control group 1(normal rats) Therefore,  these medicinal plants walnut seeds showed significant increase in their testosterone concentration, luteinizing hormone (LH) and follicle stimulating hormone (FSH) which significantly enhanced the production of reproductive hormones which enriched the fertility status of these animals.

scholarly journals Comparative Modulation of the Reproductive System by Ethanolleaf Extracts of Asystasia Gangetica Andanthocleista Vogelli in Male Wistar Rats

2020 ◽  
Vol 2 (3) ◽  
Author(s):  
Simeon I. Egba ◽  
C. O. Okonkwo ◽  
H. C. Omeoga ◽  
I. E. Ekong
Keyword(s):  
Luteinizing Hormone ◽  
Reproductive System ◽  
Wistar Rats ◽  
Follicle Stimulating Hormone ◽  
Reproductive Hormones ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Albino Rats ◽  
Male Wistar Rats ◽  
Stimulating Hormone

A number of medicinal plants have been found to influence the level of reproductive hormones and thus affect fertility in the overall. This work evaluated the effects of ethanol leaf extracts of A. gangetica and A. vogelli respectively on some reproductive system parameters in male Wistar rats. A total of sixteen (16) male albino rats were grouped into four (four rats in each group): group A served as normal control, group B received Immunace (Vitabiotics) group C and D received 400 mg/kg body weight of A. gangetica and A. vogelli extracts respectively. Extracts were administered orally to rats for 21 days, after which they were sacrificed by cervical dislocations and blood samples drawn by cardiac puncture. The effect of the extracts on testosterone, follicle stimulating hormone, luteinizing hormone and semen analysis of the test rats were determined using enzyme linked immunosorbent assay (ELISA) and standard techniques. Data collected were analyzed using Graph pad prism V6 and p values < 0.05 were adopted as significant. There was no significant (p>0.05) change in testosterone, FSH and luteinizing hormones in the group administered A. gangetica plant extract relative to the control. There was also no visible difference in the testes weight and sperm morphology relative to the control group. In contrast, administration of A. vogelli extract caused significant (p<0.05) decrease in testosterone and follicle stimulating hormone concentrations from: 1.12 ± 0.20 to 0.89 ± 0.05 and 1.41 ± 0.07 to 1.35 ± 0.12 respectively relative to the control group. While significant (p<0.05) decrease in luteinizing hormone (1.71 ± 0.15) was observed relative to the standard drug group (1.76 ± 0.05). A significant (p<0.05) decrease in sperm count and testes weight was also observed in rats treated with A. vogelli extract relative to the normal control.The results suggest that, A. vogelli extract may cause decreased fertility in male albino rats and could be developed further into potent male contraceptives. A. gangetica on the other hand, had no effect on male reproductive hormones.

scholarly journals Biochemical Effect of Antioxidants on Lipids and Liver Function in Experimentally-Induced Liver Damage

1997 ◽  
Vol 34 (6) ◽  
pp. 656-663 ◽  
Author(s):  
Abdel-Baset Halim ◽  
Omar El-Ahmady ◽  
Fathy Abdel-Galil ◽  
Amr Darwish ◽  
Samar Hassab-Allah ◽  
...  
Keyword(s):  
Free Radicals ◽  
Liver Function ◽  
Liver Damage ◽  
Histopathological Examination ◽  
Liver Function Tests ◽  
Organ Damage ◽  
Control Group ◽  
Albino Rats ◽  
Biochemical Effect ◽  
Experimentally Induced

Recent studies demonstrated the role of antioxidants in preventing organ damage caused by free radicals. The present study was conducted to find out the modulatory effect of some antioxidants on lipid patterns in experimentally-induced liver damage. Rats chronically intoxicated with carbon tetrachloride (CC14) were used as a model of liver injury terminating with fibrosis or cirrhosis. One hundred and sixty six albino rats were classified into five groups: one served as a control group; the second was subjected to oral administration of CC14 (200 μL/100 g body weight) twice a week; the other three groups, in addition to CC14, received oral doses of silymarin (30mg/kg), vitamin E (200 IU/kg) and vitamin C (50mg/kg) respectively. At the end of the experiment, the animals were killed, blood was collected and liver was taken for histopathological examination. Liver function tests, disturbed by CC14 were significantly modulated by antioxidants, and histopathological examination showed that antioxidants ameliorated the necrotic and fibrotic changes caused by CC14. Treatment with antioxidants was also shown to modulate the toxic effect of CC14 on the lipid profile and malondialdehyde content. Administration of antioxidants could play an important role in prophylaxis against lipid peroxidation and consequently liver fibrosis caused by free radicals.

scholarly journals INVESTIGATION OF THE ANTIOXIDANT POTENTIAL AND TOXICITY OF THE WHOLE LEAF OF SOLANUM NIGRUM IN ALBINO RATS

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Gbemisola Saibu ◽  
Oluwatosin Adu
Keyword(s):  
Nitric Oxide ◽  
Body Weight ◽  
Liver Function ◽  
Histopathological Examination ◽  
Solanum Nigrum ◽  
Antioxidant Potential ◽  
Control Group ◽  
Albino Rats ◽  
Morphological Alterations ◽  
Gluthathione Peroxidase

Introduction: Solanum nigrum is a common herb that grows wild and abundantly in open fields. Solanum nigrum has been shown to have anti-inflammatory properties. Most animal studies have been on the aqueous and alcoholic extracts of Solanum nigrum leaf, this study focuses on the whole leaf. Aims: The aim of this study was to assess the antioxidant potential and effect of the whole leaf of S. nigrum on liver function parameters in rats. Materials and Methods: Fifteen male Wistar rats were divided into three groups of 5 rats each. Solanum nigrum leaves rinsed,air-dried, milled and administered orally to the rats at two doses (100mg/kg and 200mg/kg body weight in 1% CMC) for seven days. The control group received 1ml of 1% CMC orally for seven days. On the eight day, animals were sacrificed and cardiac blood collected into plain bottles. Standard methods were used to determine serum nitric oxide, lipid peroxidation, gluthathione peroxidase, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and total bilirubin. One gram portion of the organs (heart, kidney, liver and spleen) were fixed in formal saline for histopathological examination of 2 animals per group. Results: Solanum nigrum had an LD50 greater than 1000mg/kg body weight without symptoms associated with toxicity. Nitric oxide concentration was significantly lower (p<0.05) in the S. nigrum groups (92.0-118.33mM) compared to the control (238.00mM) whereas gluthathione peroxidase was significantly increased (p<0.05) compared to the control group. The effects on these parameters were not dose dependent. There was no significant change in liver function parameters in all the groups. The histopathological screening of the control and test groups showed normal profile indicating no morphological alterations in the heart, liver, kidney and spleen of the rats. Conclusion: This results indicates the antioxidant effect of Solanum nigrum leaf. It also showed that the consumption of S. nigrum leaves at the chosen doses had no adverse effect on the organs of the rats. Further work will need to be done at higher doses, for longer duration and on both sexes of animals.

Impact of cefepime on hematological, immunological and oxidant/antioxidant parameters in rats experimentally infected with E. coli ATCC 25922

2020 ◽  
Vol 21 (1) ◽  
pp. 36-45
Author(s):  
Huda Elbaz ◽  
Mohamed Hamed ◽  
Fatma Abdelhamid ◽  
Osama Abdalla
Keyword(s):  
Liver Function ◽  
Oxidative Damage ◽  
Hemoglobin Concentration ◽  
Treated Group ◽  
Serum Glucose ◽  
Infected Group ◽  
Fixed Dose ◽  
Albino Rats ◽  
Immunological Parameters ◽  
Antioxidant Parameters

Objective: To evaluate the effect of cefepime on hematological changes, immunological disorders and hepatic oxidative damage in rats experimentally infected with E.coli ATCC 25922. Design: Randomized controlled experimental study. Animals: Thirty-two adult male albino rats weighting150-200 g. Procedures: Rats used for this study were randomly assigned into 4 equal groups: the control one, E.coli infected group (1×108CFU/I/P/once), the cefepime treated group (45 mg/kg bw/I/M/day) for 5 days and the E.coli infected group that treated with cefepime 24h after bacterial inoculation as previously described. Hematological and immunological parameters, liver function biomarkers and hepatic oxidative stress and antioxidant markers were determined. Results: Our result revealed that E.coli infection induced a significant elevation in the erythrocytes count, hemoglobin concentration, PCV% and total leukocytic count (TLC) (P < 0.05). In the same respect, liver function biomarkers, serum glucose, total cholesterol, and triglyceride levels as well hepatic malondialdehyde (MDA), nitric oxide (NO), TNF-α, IL-10, and lysozyme activity were significantly increased compared to the control rats (P < 0.05). In contrast, hepatic reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were decreased significantly (P < 0.05). Cefepime treatment in E.coli + CFPM group reduced the elevated eythrogram, TLC and liver function biomarkers. Cefepime also ameliorated the oxidative damage and inflammatory response induced by E.coli infection. Conclusion and clinical relevance: Cefepime is safe when administered in a fixed-dose and possess antioxidant that contributes to improve efficacy against adverse effect induced by E.coli ATCC 25922 infection.

Effect of cefepime on hematological, immunological and oxidant/antioxidant parameters in rats experimentally infected with E. coli ATCC 25922

2020 ◽  
Vol 21 (1) ◽  
pp. 36-45
Author(s):  
Huda Elbaz
Keyword(s):  
Liver Function ◽  
Oxidative Damage ◽  
Hemoglobin Concentration ◽  
Treated Group ◽  
Serum Glucose ◽  
Infected Group ◽  
Fixed Dose ◽  
Albino Rats ◽  
Immunological Parameters ◽  
Antioxidant Parameters

Objective: To evaluate the effect of cefepime on hematological changes, immunological disorders and hepatic oxidative damage in rats experimentally infected with E.coli ATCC 25922. Design: Randomized controlled experimental study. Animals: Thirty-two adult male albino rats weighting150-200 g. Procedures: Rats used for this study were randomly assigned into 4 equal groups: the control one, E.coli infected group (1×108CFU/I/P/once), the cefepime treated group (45 mg/kg bw/I/M/day) for 5 days and the E.coli infected group that treated with cefepime 24h after bacterial inoculation as previously described. Hematological and immunological parameters, liver function biomarkers and hepatic oxidative stress and antioxidant markers were determined. Results: Our result revealed that E.coli infection induced a significant elevation in the erythrocytes count, hemoglobin concentration, PCV% and total leukocytic count (TLC) (P < 0.05). In the same respect, liver function biomarkers, serum glucose, total cholesterol, and triglyceride levels as well hepatic malondialdehyde (MDA), nitric oxide (NO), TNF-α, IL-10, and lysozyme activity were significantly increased compared to the control rats (P < 0.05). In contrast, hepatic reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were decreased significantly (P < 0.05). Cefepime treatment in E.coli + CFPM group reduced the elevated eythrogram, TLC and liver function biomarkers. Cefepime also ameliorated the oxidative damage and inflammatory response induced by E.coli infection. Conclusion and clinical relevance: Cefepime is safe when administered in a fixed-dose and possess antioxidant that contributes to improve efficacy against adverse effect induced by E.coli ATCC 25922 infection.

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