Preparation and antibacterial properties of an AgBr@SiO2/GelMA composite hydrogel

2022 ◽  
Author(s):  
Bo Li ◽  
Hong Li ◽  
Haocheng Yang ◽  
Yue Shu ◽  
Kejiang Li ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Uv Light ◽  
Antibacterial Properties ◽  
Silver Ions ◽  
Skin Wound ◽  
Wound Dressings ◽  
Sprague Dawley ◽  
Sio2 Microspheres ◽  
Modified Stöber Method

Abstract Pure gelatin hydrogels lack antibacterial function and have poor mechanical properties, which restrict their application in wound dressings. In this study, nanosized silver bromide-doped mesoporous silica (AgBr@SiO2) microspheres with hollow structures were prepared by a modified Stober method. The novel microspheres can not only release silver ions to treat bacteria but also release drugs to treat skin wound. Furthermore, AgBr@SiO2 microspheres were modified with propyl methacrylate, incorporated into methacrylated gelatin (GelMA), and crosslinked by UV light to prepare AgBr@SiO2/GelMA dressings consisting of composite hydrogels. The results showed that the AgBr@SiO2 microspheres could enhance the mechanical properties of the hydrogels. With the increase in the AgBr@SiO2 concentration from 0.5 to 1 mg/mL, the dressings demonstrated effective antimicrobial activity against both Staphylococcus aureus and Escherichia coli. Furthermore, full-thickness skin wounds in vivo wound healing studies with Sprague–Dawley rats were evaluated. When treated with AgBr@SiO2/GelMA containing 1 mg/mL AgBr@SiO2, only 15% of the wound area left on day 10. Histology results also showed the epidermal and dermal layers were better organized. These results suggest that AgBr@SiO2/GelMA-based dressing materials could be promising candidates for wound dressings.

In Vivo and ex Vivo Approaches to Studying the Biomechanical Properties of Healing Wounds in Rat Skin

2013 ◽  
Vol 135 (10) ◽  
Author(s):  
Clare Y. L. Chao ◽  
Gabriel Y. F. Ng ◽  
Kwok-Kuen Cheung ◽  
Yong-Ping Zheng ◽  
Li-Ke Wang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Ex Vivo ◽  
Normal Skin ◽  
Biomechanical Properties ◽  
Skin Wound ◽  
Sprague Dawley ◽  
Rat Skin ◽  
Mechanical Stiffness ◽  
Air Jet

An evaluation of wound mechanics is crucial in reflecting the wound healing status. The present study examined the biomechanical properties of healing rat skin wounds in vivo and ex vivo. Thirty male Sprague-Dawley rats, each with a 6 mm full-thickness circular punch biopsied wound at both posterior hind limbs were used. The mechanical stiffness at both the central and margins of the wound was measured repeatedly in five rats over the same wound sites to monitor the longitudinal changes over time of before wounding, and on days 0, 3, 7, 10, 14, and 21 after wounding in vivo by using an optical coherence tomography-based air-jet indentation system. Five rats were euthanized at each time point, and the biomechanical properties of the wound tissues were assessed ex vivo using a tensiometer. At the central wound bed region, the stiffness measured by the air-jet system increased significantly from day 0 (17.2%), peaked at day 7 (208.3%), and then decreased progressively until day 21 (40.2%) as compared with baseline prewounding status. The biomechanical parameters of the skin wound samples measured by the tensiometer showed a marked reduction upon wounding, then increased with time (all p < 0.05). On day 21, the ultimate tensile strength of the skin wound tissue approached 50% of the normal skin; while the stiffness of tissue recovered at a faster rate, reaching 97% of its prewounded state. Our results suggested that it took less time for healing wound tissues to recover their stiffness than their maximal strength in rat skin. The stiffness of wound tissues measured by air-jet could be an indicator for monitoring wound healing and contraction.

scholarly journals Early Biofilm Formation on UV Light Activated Nanoporous TiO2SurfacesIn Vivo

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Nagat Areid ◽  
Eva Söderling ◽  
Johanna Tanner ◽  
Ilkka Kangasniemi ◽  
Timo O. Närhi
Keyword(s):  
Titanium Alloy ◽  
Biofilm Formation ◽  
Uv Light ◽  
Antibacterial Properties ◽  
Mutans Streptococci ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Biofilm Development

Purpose. To explore earlyS. mutansbiofilm formation on hydrothermally induced nanoporous TiO2surfacesin vivoand to examine the effect of UV light activation on the biofilm development.Materials and Methods. Ti-6Al-4V titanium alloy discs (n = 40) were divided into four groups with different surface treatments: noncoated titanium alloy (NC); UV treated noncoated titanium alloy (UVNC); hydrothermally induced TiO2coating (HT); and UV treated titanium alloy with hydrothermally induced TiO2coating (UVHT).In vivoplaque formation was studied in 10 healthy, nonsmoking adult volunteers. Titanium discs were randomly distributed among the maxillary first and second molars. UV treatment was administered for 60 min immediately before attaching the discs in subjects’ molars. Plaque samples were collected 24h after the attachment of the specimens. Mutans streptococci (MS), non-mutans streptococci, and total facultative bacteria were cultured, and colonies were counted.Results. The plaque samples of NC (NC + UVNC) surfaces showed over 2 times more oftenS. mutanswhen compared to TiO2surfaces (HT + UVHT), with the number of colonized surfaces equal to 7 and 3, respectively.Conclusion. Thisin vivostudy suggested that HT TiO2surfaces, which we earlier showed to improve blood coagulation and encourage human gingival fibroblast attachmentin vitro, do not enhance salivary microbial (mostly mutans streptococci) adhesion and initial biofilm formation when compared with noncoated titanium alloy. UV light treatment provided Ti-6Al-4V surfaces with antibacterial properties and showed a trend towards less biofilm formation when compared with non-UV treated titanium surfaces.

scholarly journals Gelatin-Based Hybrid Scaffolds: Promising Wound Dressings

Polymers ◽  
2021 ◽  
Vol 13 (17) ◽  
pp. 2959 ◽  
Author(s):  
Sindi P. Ndlovu ◽  
Kwanele Ngece ◽  
Sibusiso Alven ◽  
Blessing A. Aderibigbe
Keyword(s):  
Mechanical Properties ◽  
Antimicrobial Activity ◽  
Wound Healing ◽  
Antimicrobial Agents ◽  
Healing Process ◽  
Wound Dressings ◽  
Wound Healing Process ◽  
Delayed Wound Healing

Wound care is a major biomedical field that is challenging due to the delayed wound healing process. Some factors are responsible for delayed wound healing such as malnutrition, poor oxygen flow, smoking, diseases (such as diabetes and cancer), microbial infections, etc. The currently used wound dressings suffer from various limitations, including poor antimicrobial activity, etc. Wound dressings that are formulated from biopolymers (e.g., cellulose, chitin, gelatin, chitosan, etc.) demonstrate interesting properties, such as good biocompatibility, non-toxicity, biodegradability, and attractive antimicrobial activity. Although biopolymer-based wound dressings display the aforementioned excellent features, they possess poor mechanical properties. Gelatin, a biopolymer has excellent biocompatibility, hemostatic property, reduced cytotoxicity, low antigenicity, and promotes cellular attachment and growth. However, it suffers from poor mechanical properties and antimicrobial activity. It is crosslinked with other polymers to enhance its mechanical properties. Furthermore, the incorporation of antimicrobial agents into gelatin-based wound dressings enhance their antimicrobial activity in vitro and in vivo. This review is focused on the development of hybrid wound dressings from a combination of gelatin and other polymers with good biological, mechanical, and physicochemical features which are appropriate for ideal wound dressings. Gelatin-based wound dressings are promising scaffolds for the treatment of infected, exuding, and bleeding wounds. This review article reports gelatin-based wound dressings which were developed between 2016 and 2021.

scholarly journals Influence of silver-ion-containing pharmacotherapeutic system for repair of anterior abdominal wall on connective tissue formation in experiment

2019 ◽  
Vol 5 (3) ◽  
pp. 57-66
Author(s):  
Ekaterina S. Mishina ◽  
Maria Zatolokina ◽  
Anastasia V. Gureeva ◽  
Vladimir V. Gureev ◽  
Karina A. Elagina ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Abdominal Wall ◽  
Anterior Abdominal Wall ◽  
Rapid Change ◽  
Antibacterial Properties ◽  
Silver Ions ◽  
Modern Medicine ◽  
Connective Tissue Capsule

Introduction: In modern medicine new pharmacotherapeutic systems significantly reducing the risk of complications are being actively searched for. The study undertaken was aimed at studying one of such systems consisting of a prosthesis coated with silver ions. Materials and methods: The material for the study was standard endoprostheses produced by Lintex LLC (St.-Petersburg) for plastic repair of the anterior abdominal wall: Esfil and Uniflex, as well as Plasmofilter produced by Plasmofilter JSC (St.-Petersburg) and a pharmacotherapeutic system containing silver ions (FCS) applied to the experimental samples of endoprostheses – Esfil Ag and Unifleks Ag (patent RU 2473369C1), which were implanted into male Wistar rats. The results were evaluated using morphological, morphometric, immunohistochemical, ionometric, microbiological and statistical methods. Results and discussion: As a result, it was found that the use of the FCS leads to a more rapid change in inflammation phases. The formation of a mature connective tissue capsule with a thickness of 4.5±0.01 mm was observed by Day 30. The study of the cellular component of the periprosthetic tissue revealed the prevalence of resident cells. The proliferative activity of fibroblastic cells when using FCS over 14 days was statistically significantly (p≤0.05) 3.5-time higher. Microbiological studies confirmed the antibacterial properties of FCS in vivo and in vitro. Conclusion: The use of FCS contributes to the acceleration of reparative processes, earlier resolution of inflammation and stimulation of collagenesis both under sterile conditions and under microbial conditions.

Novel Nanofibrous Scaffold to Improve Wound Healing

2013 ◽  
Author(s):  
Eva Yi-Wah Mak ◽  
Wallace Woon-Fong Leung
Keyword(s):  
Escherichia Coli ◽  
Mechanical Properties ◽  
Staphylococcus Aureus ◽  
Acidic Solution ◽  
Antibacterial Properties ◽  
Chitosan Solution ◽  
Skin Wound ◽  
Gram Negative ◽  
Native Skin ◽  
Improve Wound Healing

An antibacterial and biocompatible scaffold for fibroblasts proliferation based on chitosan has been developed. Chitosan solution is electrospun into uniform fibers of 100–200 nm in diameter that mimic the extracellular matrix of human skin. The fibrous mats are successfully cross-linked to be stable in acidic solution, which can be used to treat acute wounds. The crosslinked fibrous mats display antibacterial properties toward Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. The mechanical properties of fibrous mats are shown to be comparable to native skin dermis which protects the skin wound.

scholarly journals Degradable tough chitosan dressing for skin wound recovery

2020 ◽  
Vol 9 (1) ◽  
pp. 1576-1585
Author(s):  
Yan Kong ◽  
Xiaoxuan Tang ◽  
Yahong Zhao ◽  
Xiaoli Chen ◽  
Ke Yao ◽  
...  
Keyword(s):  
Water Absorption ◽  
Wound Dressing ◽  
Antibacterial Properties ◽  
High Water ◽  
Skin Wound ◽  
Wound Dressings ◽  
Wound Recovery ◽  
Recovery And Healing ◽  
Optimized Treatment ◽  
Better Than

Abstract The performance of wound dressing determines the effect of wound closure and recovery. Water absorption and bacteriostasis of wound dressings play an important role in wound recovery and healing. In this study, an optimized chitosan wound dressing-tough chitosan dressing (TCS) with high water absorption, high bacteriostasis, and degradability was developed. The chemical structure of chitosan remained stable during the process of optimized treatment, and an increase in mechanical properties was obtained for the dressing. After optimization, the water absorption and antibacterial properties of the chitosan dressing were greatly improved, which is significantly better than sodium alginate dressing. The authors believe that TCS dressing with high hygroscopicity and high bacteriostasis has great potential application value in the field of wound recovery and healing.

scholarly journals Highly Adhesive Antibacterial Bioactive Composite Hydrogels With Controllable Flexibility and Swelling as Wound Dressing for Full-Thickness Skin Healing

2021 ◽  
Vol 9 ◽  
Author(s):  
Guanhua Lan ◽  
Suping Zhu ◽  
Dong Chen ◽  
Hua Zhang ◽  
Lijin Zou ◽  
...  
Keyword(s):  
Cell Activity ◽  
Antibacterial Properties ◽  
Wound Dressings ◽  
Great Promise ◽  
Skin Defect ◽  
Swelling Ratio ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin

Polyzwitterionic hydrogels as skin wound dressings have been extensively studied owing to their superior antibacterial properties and skin adhesiveness, but their practical applications still suffer from a low adhesion strength and a high swelling ratio, which hinder the application of hydrogel for cutaneous healing. Here, we developed a novel biocompatible poly[2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide (PolySBMA) composite hydrogel with high stretchability, low swelling, strong skin adhesiveness, and antibacterial effect for enhancing wound healing. Naturally rigid polymers including quaternized chitosan methacrylate (QCSMA) and gelatin methacrylate (GelMA) are used as bioactive cross-linkers to endow PolySBMA/QCSMA/GelMA (SQG) hydrogel with a low swelling ratio and high bioactivity. The optimized hydrogel has excellent mechanical flexibility, with the ultimate tensile strength, tensile strain, modulus, and toughness of up to 344.5 kPa, 364%, 14.7 kPa, and 33.4 kJ m−3, respectively. The adhesiveness of the hydrogel to the skin tissue is as high as 38.2 kPa, which is critical for stopping the bleeding from the wound. The synergistic contributions from the PolySBMA and QCSMA endow hydrogel with good antibacterial properties against both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. Moreover, the natural polymer cross-linked polyzwitterionic hydrogel shows good cell activity, hemocompatibility, and histocompatibility. The in vivo full-thickness skin defect model demonstrates that the SQG hydrogel efficiently improves the granulation tissue formation and collagen deposition. In summary, such superiorly skin-adhesive antibacterial biocompatible hydrogel with controllable flexibility and swelling holds great promise as wound dressings for acute wounds.

Tough, Adhesive, Self-healable, and Antibacterial Plant-inspired Hydrogel Based on Pyrogallol-Borax Dynamic Cross-linking

2021 ◽  
Author(s):  
Chao Ma ◽  
Huiwen Pang ◽  
Hongguang Liu ◽  
Qian Yan ◽  
Jianzhang Li ◽  
...  
Keyword(s):  
Antibacterial Properties ◽  
Wearable Devices ◽  
Skin Wound ◽  
Wound Dressings ◽  
Cross Linking ◽  
Self Healing ◽  
Electronic Skin

Multifunctional hydrogels that integrate stretchability, adhesion, self-healing, and antibacterial properties may find use in a variety of fields including electronic skin, wound dressings, and wearable devices; however, traditional hydrogels often...

In vitro and in vivo antibacterial properties of peptide AMC-109 impregnated wound dressings and gels

2021 ◽  
Author(s):  
Joakim Håkansson ◽  
Jorunn Pauline Cavanagh ◽  
Wenche Stensen ◽  
Bjarte Mortensen ◽  
John-Sigurd Svendsen ◽  
...  
Keyword(s):  
Antibacterial Properties ◽  
Wound Dressings

scholarly journals Evaluation of a Model Photo-Caged Dehydropeptide as a Stimuli-Responsive Supramolecular Hydrogel

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 704
Author(s):  
Peter J. Jervis ◽  
Loic Hilliou ◽  
Renato B. Pereira ◽  
David M. Pereira ◽  
José A. Martins ◽  
...  
Keyword(s):  
Self Assembly ◽  
Uv Light ◽  
Aqueous Media ◽  
Intramolecular Interactions ◽  
Biologically Active ◽  
Wound Dressings ◽  
Supramolecular Hydrogel ◽  
On Demand ◽  
Proteolytic Resistance

Short peptides capped on the N-terminus with aromatic groups are often able to form supramolecular hydrogels, via self-assembly, in aqueous media. The rheological properties of these readily tunable hydrogels resemble those of the extracellular matrix (ECM) and therefore have potential for various biological applications, such as tissue engineering, biosensors, 3D bioprinting, drug delivery systems and wound dressings. We herein report a new photo-responsive supramolecular hydrogel based on a “caged” dehydropeptide (CNB-Phe-ΔPhe-OH 2), containing a photo-cleavable carboxy-2-nitrobenzyl (CNB) group. We have characterized this hydrogel using a range of techniques. Irradiation with UV light cleaves the pendant aromatic capping group, to liberate the corresponding uncaged model dehydropeptide (H-Phe-ΔPhe-OH 3), a process which was investigated by 1H NMR and HPLC studies. Crucially, this cleavage of the capping group is accompanied by dissolution of the hydrogel (studied visually and by fluorescence spectroscopy), as the delicate balance of intramolecular interactions within the hydrogel structure is disrupted. Hydrogels which can be disassembled non-invasively with temporal and spatial control have great potential for specialized on-demand drug release systems, wound dressing materials and various topical treatments. Both 2 and 3 were found to be non-cytotoxic to the human keratinocyte cell line, HaCaT. The UV-responsive hydrogel system reported here is complementary to previously reported related UV-responsive systems, which are generally composed of peptides formed from canonical amino acids, which are susceptible to enzymatic proteolysis in vivo. This system is based on a dehydrodipeptide structure which is known to confer proteolytic resistance. We have investigated the ability of the photo-activated system to accelerate the release of the antibiotic, ciprofloxacin, as well as some other small model drug compounds. We have also conducted some initial studies towards skin-related applications. Moreover, this model system could potentially be adapted for on-demand “self-delivery”, through the uncaging of known biologically active dehydrodipeptides.

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