scholarly journals Fertilization Rate of Giant Grouper (Epinephelus lanceolatus, Bloch 1790) Sperm Post-cryopreservation and Application on Hybridization in Different Grouper Species

2021 ◽  
Vol 748 (1) ◽  
pp. 012001
Author(s):  
W Widyaningsih ◽  
Abinawanto ◽  
D Susandi
Keyword(s):  
Chorionic Gonadotropin ◽  
Fertilization Rate ◽  
Sperm Cryopreservation ◽  
Epinephelus Fuscoguttatus ◽  
Tiger Grouper

Abstract Fertilization was doing to measure the success of sperm cryopreservation because it was more informative. Sperm cryopreservation was expected to get sperm that successfully fertilize the egg well. The aim of this study was to determine the sperm fertilization ability of giant grouper (Epinephelus lanceolatus, Bloch 1790) 48 hours post-cryopreservation in fertilizing tiger grouper eggs (Epinephelus fuscoguttatus, Forskal 1775). Fertilization was carried out artificially using cryopreservation sperm with 6% of glycerol and palm dates concentrates (0%; 5%; 10%; 15%; 20%; and 25%). The tiger grouper brood was previously injected with hormone chorionic gonadotropin at a dose of 500 IU/kg. The results showed that there were significant differences (P < 0.05) on fertilization of tiger grouper eggs with sperm of giant grouper which were cryopreserved with 6% of glycerol and various concentrations of palm date concentrates. Sperm which was cryopreserved with 6% of glycerol and 10% of palm date concentrate has the best fertility ability by showing the highest value of 66.25 ± 3.23%.

scholarly journals Priming with a gonadotropin-releasing hormone agonist before immature oocyte retrieval may improve maturity of oocytes and outcome in in vitro maturation (IVM) cycle: a case report

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
A. Smirnova ◽  
M. Anshina ◽  
E. Shalom Paz ◽  
A. Ellenbogen
Keyword(s):  
Fertility Preservation ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Oocyte Retrieval ◽  
Fertilization Rate ◽  
Gonadotropin Releasing Hormone ◽  
Releasing Hormone ◽  
Nuclear Maturation ◽  
Gonadotropin Releasing Hormone Agonist

Abstract Background The concept of using a gonadotropin-releasing hormone agonist (GnRH-a) instead of human chorionic gonadotropin for triggering ovulation in patients treated with an antagonist protocol for in vitro fertilization (IVF) has become a routine clinical practice. It may promote oocyte nuclear maturation, resumption of meiosis and cumulus expansion. It seems that this attempt could be beneficial in an in vitro maturation (IVM) oocyte cycle performed for polycystic ovarian syndrome as well as for other indications such as urgent fertility preservation in patients with malignancies or unusual indications. Case presentation We present the case of a Caucasian patient who needed fertility preservation when routine natural IVF treatment did not yield oocyte retrieval, followed by three IVM cycles, priming ovulation with a GnRH-a. In total, 12 oocytes were obtained, all matured 4.5 hours after incubation in maturation media. The fertilization rate after intracytoplasmic sperm injection was 83%. Six good-quality embryos were vitrified. Conclusions It seems that triggering with a GnRH-a in selected cases may replace human chorionic gonadotropin in IVM of oocytes and could be highly beneficial in terms of obtaining high-grade embryos and possible pregnancy.

scholarly journals ANALISIS KESESUAIAN PERAIRAN UNTUK BUDIDAYA IKAN KERAPU MACAN (Epinephelus fuscoguttatu) DI PERAIRAN PULAU TEGAL KECAMATAN TELUK PANDAN KABUPATEN PESAWARAN

2018 ◽  
Vol 6 (2) ◽  
pp. 705 ◽  
Author(s):  
Glenn Valentino ◽  
Abdullah Aman Damai ◽  
Herman Yulianto
Keyword(s):  
Water Quality ◽  
Secondary Data ◽  
Fish Farming ◽  
Primary Data ◽  
Marine Aquaculture ◽  
Quality Of Waters ◽  
Epinephelus Fuscoguttatus ◽  
Tiger Grouper ◽  
Physical And Chemical

Tiger grouper is a marine aquaculture commodities that has good prospects to be developed. One of the areas that could potentially be used as a place of tiger grouper fish farming is the Tegal Island, Pesawaran. The purpose of this research was to analyze the level of water quality for fish farming tiger grouper (Epinephelus fuscoguttatus). This research was carried out in March-May 2017 descriptive analytic nature by doing the observations on the quality of waters which include physical and chemical parameters. The data that used in this research sourced from primary data about the suitability waters of the island while the secondary data is form in research location maps. There were 4 sampling stations which determined with global positioning system (GPS). The results showed that the water quality which is owned by the island's waters on station 1, 2, 3 and 4 were in the quite appropriate category for the cultivation of tiger grouper.

Hybridization of tiger grouper (Epinephelus fuscoguttatus ♀) x giant grouper (Epinephelus lanceolatus ♂) using cryopreserved sperm

Cryobiology ◽  
2020 ◽  
Vol 95 ◽  
pp. 84-89
Author(s):  
Bin Fan ◽  
Sen Yang ◽  
Le Wang ◽  
Xinghan Chen ◽  
Xiaochun Liu ◽  
...  
Keyword(s):  
Epinephelus Fuscoguttatus ◽  
Tiger Grouper ◽  
Cryopreserved Sperm

scholarly journals Effects of Ascorbic Acid and β-1,3-Glucan on Survival, Physiological Response and Flesh Quality of Cultured Tiger Grouper (Epinephelus fuscoguttatus) during Simulated Transport in Water

Biology ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 37 ◽  
Author(s):  
Bo Wu ◽  
Qi Wang ◽  
Jie Cao ◽  
Jun Mei ◽  
Jing Xie
Keyword(s):  
Ascorbic Acid ◽  
Survival Rate ◽  
Stress Response ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Flesh Quality ◽  
Epinephelus Fuscoguttatus ◽  
Tiger Grouper

Transport in water is the most common method for achieving high survival rates when transporting cultured fish in China; yet, transport success relies on proper water quality and conditions. This research was designed to explore the effects of ascorbic acid and β-1,3-glucan on survival, physiological responses, and flesh quality of farmed tiger grouper (Epinephelus fuscoguttatus) during simulated transport. The transport water temperature for live tiger grouper was 15 °C, which had the highest survival rate, the lowest stress response, and metabolic rate, and this will reduce the susceptibility to diseases. It is stated that β-1,3-glucan influences the changes of cortisol content, heat shock protein 70, IL-1β, and IgM transcription levels during simulated transport. Rather than using ascorbic acid alone (the A-group), β-1,3-glucan (3.2 mg/L) in the presence of ascorbic acid (25 mg/L) can effectively reduce the increase of transport-induced serum cortisol content, heat shock protein 70, and IL-1β, but stimulated IgM. 25 mg/L ascorbic acid and 3.2 mg/L β-1,3-glucan had no obvious effect on the nutritional indexes and flavor of live tiger grouper; however, these can effectively reduce the stress response, improve the innate immune activity, and ensure a higher survival rate.

scholarly journals The Embryonic Develompent and Hatching Rate of Cantang Grouper (Epinephelus fuscoguttatus, Forskall 1775 x Epinephelus lanceolatus, Bloch 1790)

2020 ◽  
Vol 3 (2) ◽  
pp. 304
Author(s):  
Albet Surya Kembara ◽  
Putri Desi Wulan Sari
Keyword(s):  
Embryonic Development ◽  
Production Process ◽  
Hatching Rate ◽  
Development Phase ◽  
Maintenance Period ◽  
Epinephelus Fuscoguttatus ◽  
Tiger Grouper ◽  
Production Value ◽  
Hatching Eggs ◽  
Technological Opportunities

Cantang grouper is a grouper species produced from the hybridization of Kertang grouper and Tiger grouper.  The hybridization,besides increasing species diversification, also has the prospect of cultivation that has the opportunity to increase fishery production in the future. The technique of maintaining grouper larvae needs to be known to be able to increase the production value of the grouper larvae and create technological opportunities in the production process. The embryonic development phase of cantang grouper egg consists of multicellular phase, blastula, gastrula, separation of the tail from the egg sac,  the movement of embryo, internal organ formation and the hatching eggs. During the two months maintenance period, the Hatching Rate (HR) larvae were found to be 80%.

scholarly journals Selection of probiotic bacteria from the coral reef and tiger grouper fish (Epinephelus foscoguttatus) farming environment

2013 ◽  
Vol 11 (2) ◽  
pp. 109
Author(s):  
. Ilmiah ◽  
. Sukenda ◽  
. Widanarni ◽  
Enang Harris
Keyword(s):  
Coral Reef ◽  
Probiotic Bacteria ◽  
Pathogenicity Test ◽  
Zone Of Inhibition ◽  
Culture Methods ◽  
Lipase Enzyme ◽  
Test Zone ◽  
Epinephelus Fuscoguttatus ◽  
Tiger Grouper ◽  
Selection Of

<p>The use of probiotic bacteria is one of the methods in aquaculture to control infectious diseases. This study aims to obtain candidates probiotic bacteria from the coral reef and tiger grouper aquaculture environment (<em>Epinephelus fuscoguttatus</em>). Steps of the study included of: 1) isolation of candidates probiotic bacteria, 2) selection of candidates probiotic bacteria: inhibition test and co-culture methods, 3) enzymatic test, 4) the growth of bacteria test 5) pathogenicity test and 6) the molecular identification of probiotic bacteria. The results showed that of 124 isolates in test zone of inhibition, the four isolates with the widest zone of inhibition of isolate K7, K8, K21, and T4, a total of 51 isolates on co-culture methods, the four isolates were able to suppress the growth of pathogenic <em>Vibrio</em> bacteria, isolates K15, K68, K55, and T36. The results of hydrolyzing the highest protease amylase and lipase enzyme, respectively isolates K15 and T36; isolates T41 and K8; isolates K55 and K68. Candidates probiotic bacteria reached the exponential phase at 16‒18 h. The results of pathogenicity test isolate K7, K8, T36, and T41 provided the highest survival that can be used as candidates probiotics in the tiger grouper.</p> <p>Keywords: selection, probiotics, tiger grouper</p>

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