scholarly journals Acclimatization of transformed seaweed Kappaphycus alvarezii carrying lysozyme gene in culture flask and the cultivation in floating net cage in Pangkep coastal

2021 ◽  
Vol 890 (1) ◽  
pp. 012031
Author(s):  
E Suryati ◽  
A Tenriulo ◽  
S Fadilah
Keyword(s):  
Kappaphycus Alvarezii ◽  
Mesh Size ◽  
Daily Growth ◽  
Double Line ◽  
Culture Flask ◽  
Lysozyme Gene ◽  
Cage Size ◽  
Net Cage ◽  
Net Cages

Abstract The in vitro transformation of the lysozyme gene in seaweed K. alvarezii has been successfully executed to increase the viability against ice-ice disease. There were two major stages in this research; (1) transformation of lysozyme gene in seaweed K. alvarezii which was carried out on laboratory scale and the cultivation of gene-transformed explants in the culture flask stored in “culture chamber”; (2) the acclimatization in floating net cages of green nets (mesh size of 1 mm) with cage size of 50 x 50 x 50 cm, the population density of 200 explants and cultivated for two weeks. The explants were then transferred to blue nets (mesh size of 2 mm) with a cage size of 50 x 50 x 50 cm for four weeks of rearing. The plants were then enlarged using a long-line method in the floating net cage, by tying the seaweed using a double line with a gap of 15 cm each. The measurement of weight, bud lengths, and water quality was carried out within 2 weeks. The result shows that the daily growth rate of the transformed seaweed during the regeneration stage in the culture flask was around 0.33-0.4%/day, meanwhile during the acclimatization stage in the green nets the was 0.65-1.6%/day, and even more, increased during the acclimatization stage in the blue nets with DGR of 2.28-2.3%/day. During the enlargement stage in the floating net cages, the lysozyme-transformed seaweed showed an even higher DGR with a value of 3.2-8.2%/day. The results of the integration of the lysozyme gene in seaweed were indicated by the presence of a 670 bp of amplification products, that is the same total length of the 35 S-F promoter fragments and Nos T-R in the expression vector. Based on these results, the lysozyme gene was successfully transformed in K. alvarezii seaweed.

scholarly journals The Structural Response Investigation of Modular Pontoon Collar Floating Cage due to Current Load to Support Fish Farming Activities

2018 ◽  
Vol 159 ◽  
pp. 02054
Author(s):  
Aulia Windyandari ◽  
Ahmad Fauzan Zakki
Keyword(s):  
Structural Response ◽  
Fish Farming ◽  
Strength Analysis ◽  
Current Load ◽  
Fish Welfare ◽  
Explicit Finite Element ◽  
Finite Element Software ◽  
Cage Size ◽  
Net Cage ◽  
Net Cages

The size of net cages increases rapidly, reaching volumes above current experience. More exposed locations are used for fish production, introducing higher loads on the net cage and fish farm from strong water currents and large waves. Strength analysis can be a useful tool for development of net-cage designs to avoid escape of fish and ensure sufficient volume for good fish welfare and water quality. The main objectives of this research are to investigate the structural response of modular pontoon collar floating cage due to the current load to support the fish farming activities. Strength analysis was performed using commercial explicit finite element software to calculate the distribution of loads in the net cage due to current, weights and gravity. The net cage was modeled using truss elements that represented several parallel twines. Sub-elements allowed the trusses to buckle in compression, and only negligible compressive forces were seen in the numerical results. Resulting drag loads and cage volume were shown to be dependent on the net cage size and collar shape.

scholarly journals REGENERASI DAN PERBANYAKAN RUMPUT LAUT Kappaphycus alvarezii HASIL TRANSFORMASI GEN SUPEROKSIDA DISMUTASE (MaSOD)

2017 ◽  
Vol 11 (4) ◽  
pp. 321
Author(s):  
Emma Suryati ◽  
Hidayah Triana ◽  
Utut Widiastuti ◽  
Andi Tenriulo
Keyword(s):  
Oxidative Stress ◽  
Growth Hormone ◽  
Metal Contamination ◽  
Kappaphycus Alvarezii ◽  
Pcr Analysis ◽  
Culture Chamber ◽  
Culture Flask ◽  
Pcr Method ◽  
Multiple Step

Transformasi gen superoxide dismutase (MaSOD) pada rumput laut Kappaphycus alvarezii menggunakan Agrobacterium tumefacient telah dilakukan secara in vitro. Transformasi gen MaSOD ke dalam genom rumput laut diharapkan dapat mengurangi cekaman oksidatif terutama yang disebabkan oleh perubahan suhu, salinitas, dan cemaran logam di perairan. Penelitian ini bertujuan untuk regenerasi rumput laut hasil introduksi gen MaSOD dan non-transgenik pada labu kultur. Regenerasi dan perbanyakan rumput laut hasil transformasi gen MaSOD dilakukan di laboratorium pada labu kultur yang diletakkan dalam “culture chamber” yang dilengkapi dengan aerasi menggunakan media kultur yang diperkaya dengan pupuk PES, Grund, Conwy, dan SSW sebagai kontrol, salinitas 20, 25, 30, 35, dan 40 g/L, pH 4, 5, 6, 7, dan 8. Intensitas cahaya antara 500-2.000 lux dengan fotoperiode terang dan gelap 8:16; 12:12; dan 16:8. Untuk merangsang pertumbuhan eksplan dilakukan pemeliharaan dengan penambahan hormon tumbuh IAA dan BAP dengan perbandingan 1:1, 1:2, dan 2:1. Penelitian dilakukan secara bertahap. Evaluasi transgenik dilakukan menggunakan teknik PCR. Hasil penelitian memperlihatkan bahwa sintasan yang paling tinggi diperoleh menggunakan media PES (94%), salinitas 30 g/L (90%), pH 7 (96%), intensitas cahaya pada 1.500 lux (80%), fotoperiode 12:12 (84%), komposisi ZPT dengan campuran IAA dan BAP dengan perbandingan 2:1. Hasil analisis PCR memperlihatkan K. alvarezii transgenik putatif mengandung transgen MaSOD sebanyak 78% dari hasil transformasi.Superoxide dismutase transformation (MaSOD) gene of seaweed Kappaphycus alvarezii mediated by Agrobacterium tumefacient has been successfully done in vitro. MaSOD genes introduced into the seaweed genome is expected to reduce oxidative stress caused by environmental conditions such as changes in temperature, salinity and metal contamination of the water. This study aimed to regenerate both the MaSOD transformed seaweed and non-transgenic in a culture flask. Regeneration and multiplication of those seaweed were conducted in a laboratory flask cultures placed in a “culture chamber” which was aerated and enriched with fertilizers PES, Grund, Conwy, and SSW as a control, salinity 20, 25, 30, 35, and 40 g/L, pH: 4, 5, 6, 7, and 8. the light intensity between 500-2000 lux, with light and dark photoperiod 8:16; 12:12; and 16:8. To stimulate the growth of explants the addition of growth hormone IAA and BAP with ratios of 1:1, 1:2 and 2:1 were performed. This study was a multiple-step of process, by which transgenic explants was identified by PCR method. The results showed that the highest survival rate was obtained using media PES (94%), salinity 30 g / L (90%), pH= 7 (96%), the intensity of light at 1500 lux (80%), photoperiod= 12: 12 (84%), and ratio of IAA and BAP 2: 1. The results of PCR analysis showed the putative K. alvarezii transgenic MaSOD was 78% of explants.

scholarly journals Selecting Fish Combination of Polyculture to Reduce Periphyton Abundance in Floating Net Cage in Cirata Reservoir, West Java, Indonesia

2017 ◽  
Vol 2 (6) ◽  
pp. 136
Author(s):  
Zahidah Hasan ◽  
Y. Setiawan ◽  
E. Rochima
Keyword(s):  
Common Property ◽  
Stocking Density ◽  
Randomized Design ◽  
Cage Size ◽  
Net Cage ◽  
Completely Randomized Design ◽  
The Individual ◽  
Net Cages ◽  
Potential Area ◽  
Silver Barb

Being a considerable potential area for aquaculture activities in the form of floating net cage, reservoir has taken on issues due to its characteristics as a common property with an open access. The problems have led to the vast growing and uncontrolled number of the net cages; one of which occurs in Cirata Reservoir. The objective of this research was to find the best combination of fishes in polyculture at Floating net cage in order to reduce periphyton abundance and to increase aquaculture productivity in Cirata Reservoir. The research has been conducted from February to March 2015. The research method used completely randomized design with three treatments and three replications. The treatments consist of fish combination of tilapia with silver barb, carp with silver barb, and black pacu with silver barb which were cultured for five weeks in floating net cage size (1 x 1 x 1) m3. The individual fish weight were approximately 10 g for tilapia, carp and silver barb, and approximately 4 g for black pacu. stocking density were 80 fishes per unit cage (40 fishes for each species) . The combination of black pacu and silver barb produced highest productivity of 459.77 gr / m3 or 82 % during five weeks. Meanwhile, combination of carp and silver barb gave lowest periphyton abundance of 28 345 cells / cm2. It can be concluded that black pacu and silver barb is the best combination due to growth rate and aquaculture productivity but not in periphyton abundance.Keywords: Polyculture, Cirata reservoir, Cageculture.

scholarly journals ANALYSIS OF RELATIONSHIP BETWEEN PRODUCTION FACTORS AND PRODUCTION TILAPIA FARMING BUSINESS IN TOBA SAMOSIR REGENCY

2019 ◽  
Vol 2 (1) ◽  
pp. 40-45
Author(s):  
Sukma Aditya Sitepu
Keyword(s):  
Secondary Data ◽  
Primary Data ◽  
Chi Square ◽  
Production Factors ◽  
Cage Size ◽  
Net Cage ◽  
Net Cages ◽  
Chi Square Analysis ◽  
Relationship Of ◽  
The Relationship

ABSTRACTThis study aims to analyze the relationship between floating net cage size, labor, feed, seeds and medicine with tilapia production in Toba Samosir Regency. The selection of the study area was carried out purposively because Toba Samosir district was a tilapia aquaculture area that had the largest harvest area in North Sumatra. The data used are secondary data and primary data obtained in the study area. The analytical method used is Chi-Square analysis using SPSS 18.0 software. The results of this research are floating net cage areas, labor, feed, seeds, and medicines used in relation to the production of tilapia fish in floating net cages in Toba Samosir District significantly. With the closeness of the relationship between the extent of Floating Net Cages, feed, medicine with a production of 78.7%, and the closeness of the relationship of seeds with production 89.2 and the closeness of labor relations with a production 70.4%.Keywords: Floating Net Cages, Production, Production Factors, Tilapia.

scholarly journals Chemistry of Tropical Eucheumatoids: Potential for Food and Feed Applications

Biomolecules ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 804
Author(s):  
Andrea Ariano ◽  
Nadia Musco ◽  
Lorella Severino ◽  
Anna De Maio ◽  
Annabella Tramice ◽  
...  
Keyword(s):  
Chemical Analysis ◽  
Inductively Coupled Plasma ◽  
Mineral Content ◽  
Kappaphycus Alvarezii ◽  
Optical Emission ◽  
Essential Elements ◽  
Feed Additive ◽  
Inductively Coupled ◽  
Food And Feed

The use of seaweeds as additives in animal nutrition may be a valid option to traditional feed as they represent a rich source of minerals, carbohydrates and antioxidants. The aim of this study was to analyze the chemical composition and in vitro antioxidant capacity of two tropical eucheumatoids, Kappaphycus alvarezii and Kappaphycus striatus, in Malaysian wild offshore waters. The chemical analysis was performed via inductively coupled plasma–optical emission spectroscopy for evaluating the concentration of toxic (Cd, Pb, Hg, As) and essential elements (Mn, Fe, Cu, Ni, Zn, Se); NMR spectroscopy was used for carrageenans investigation. Furthermore, the soluble and fat-soluble antioxidant capacities were determined by FRAP, DPPH and ABTS assays. The chemical analysis revealed a higher content of trace elements in K. alvarezii as compared to K. striatus, and both exhibited a high mineral content. No significant differences in metal concentrations were found between the two species. Both samples showed a mixture of prevailing κ- and t-carrageenans. Finally, the levels of soluble and fat-soluble antioxidants in K. alvarezii were significantly higher than in K. striatus. Our findings suggest that K. alvarezii could be used as a potential feed additive because of its favorable chemical and nutritional features.

scholarly journals Analisis ekonomi budidaya Ikan Mas (Cyprinus carpio) KJA waduk PLTA Koto Panjang

2021 ◽  
Vol 8 (1) ◽  
pp. 29
Author(s):  
Dukut Tri Sadono ◽  
Darwis AN ◽  
Zulkarnain Umar
Keyword(s):  
Cyprinus Carpio ◽  
Economic Value ◽  
Hydroelectric Power Plant ◽  
Survey Method ◽  
Sampling Location ◽  
Hydroelectric Power ◽  
Financial Feasibility ◽  
Cage Size ◽  
Net Cages ◽  
Method Factors

This study aims to analyze the factors that affect income levels, estimate economic value, analyze financial feasibility and study alternative policies for the development of goldfish (Cyprinus carpio) cultivation in floating net cages in an effort to develop fishery potential in Koto Panjang Hydroelectric Reservoir, Kampar Regency, Riau Province. The sampling location is determined based on (purposive sampling). The method used in this research is a survey method. Determination of the population in the study using the Census method. Factors that significantly influence the income of goldfish cultivators (Cyprinus carpio) at the real level α = 5% are the yield (HP) kg / unit and the number of cages (JK) unit. The surplus value of goldfish cultivation producers (Cyprinus carpio) is IDR 42,102,269,258.00 / year. The results of the analysis of the financial feasibility value (NPV) of Rp. 77,136,617.10, - / unit for the volume of cages 6 x 6 x 4. Whereas for the volume of cages 6 x 8 x 4, it has a value (NPV) which is much greater than Rp. . 173,394,632.39, - / unit. The second value (NPV) is positive based on the criteria for a feasible business investment if the value (Net B / C) is greater than 1, the value (IRR) is the same as 12.00% for both cage size categories. The sensitivity analysis shows that the cultivation of goldfish (Cyprinus carpio) KJA in the Koto Panjang Hydroelectric Power Plant is more sensitive to changes in decreased production compared to changes in decreasing price of carp feed (Cyprinus carpio). Based on the Exponential Comparative Analysis (MPE), it is found that the best alternative development policies to be developed and implemented are capital strengthening and improvement of facilities and infrastructure related to fish cultivation development.

scholarly journals Hydrogel-Coated Textile Scaffolds as Three-Dimensional Growth Support for Human Umbilical Vein Endothelial Cells (HUVECs): Possibilities as Coculture System in Liver Tissue Engineering

2002 ◽  
Vol 11 (4) ◽  
pp. 369-377 ◽  
Author(s):  
Makarand V. Risbud ◽  
Erdal Karamuk ◽  
René Moser ◽  
Joerg Mayer
Keyword(s):  
Endothelial Cells ◽  
Umbilical Vein ◽  
Three Dimensional ◽  
Mesh Size ◽  
Cell Types ◽  
Cell Number ◽  
Human Umbilical Vein ◽  
Hydrogel Matrix ◽  
Umbilical Vein Endothelial Cells

Three-dimensional (3-D) scaffolds offer an exciting possibility to develop cocultures of various cell types. Here we report chitosan–collagen hydrogel-coated fabric scaffolds with defined mesh size and fiber diameter for 3-D culture of human umbilical vein endothelial cells (HUVECs). These scaffolds did not require pre-coating with fibronectin and they supported proper HUVEC attachment and growth. Scaffolds preserved endothelial cell-specific cobblestone morphology and cells were growing in compartments defined by the textile mesh. HUVECs on the scaffold maintained the property of contact inhibition and did not exhibit overgrowth until the end of in vitro culture (day 6). MTT assay showed that cells had preserved mitochondrial functionality. It was also noted that cell number on the chitosan-coated scaffold was lower than that of collagen-coated scaffolds. Calcein AM and ethidium homodimer (EtD-1) dual staining demonstrated presence of viable and metabolically active cells, indicating growth supportive properties of the scaffolds. Actin labeling revealed absence of actin stress fibers and uniform distribution of F-actin in the cells, indicating their proper attachment to the scaffold matrix. Confocal microscopic studies showed that HUVECs growing on the scaffold had preserved functionality as seen by expression of von Willebrand (vW) factor. Observations also revealed that functional HUVECs were growing at various depths in the hydrogel matrix, thus demonstrating the potential of these scaffolds to support 3-D growth of cells. We foresee the application of this scaffold system in the design of liver bioreactors wherein hepatocytes could be cocultured in parallel with endothelial cells to enhance and preserve liver-specific functions.

Carrageenan hydrogel as a scaffold for skin-derived multipotent stromal cells delivery

2018 ◽  
Vol 33 (3) ◽  
pp. 422-434 ◽  
Author(s):  
Michele Patricia Rode ◽  
Addeli Bez Batti Angulski ◽  
Felipe Azevedo Gomes ◽  
Maiara Marques da Silva ◽  
Talita da Silva Jeremias ◽  
...  
Keyword(s):  
Human Skin ◽  
Delivery System ◽  
Stromal Cells ◽  
Kappaphycus Alvarezii ◽  
Skin Wound ◽  
Multipotent Stromal Cells ◽  
Skin Wound Healing ◽  
Matrix Deposition ◽  
Initial Recovery

Carrageenan is a thermoreversible polymer of natural origin widely used in food and pharmaceutical industry that presents a glycosaminoglycan-like structure. Herein, we show that kappa-type carrageenan extracted by a semi-refined process from the red seaweed Kappaphycus alvarezii displayed both chemical and structural properties similar to a commercial carrageenan. Moreover, both extracted carrageenan hydrogel and commercial carrageenan hydrogel can serve as a scaffold for in vitro culture of human skin-derived multipotent stromal cells, demonstrating considerable potential as cell-carrier materials for cell delivery in tissue engineering. Skin-derived multipotent stromal cells cultured inside the carrageenan hydrogels showed a round shape morphology and maintained their growth and viability for at least one week in culture. Next, the effect of the extracted carrageenan hydrogel loaded with human skin-derived multipotent stromal cells was evaluated in a mouse model of full-thickness skin wound. Macroscopic and histological analyses revealed some pointed ameliorated features, such as reduced inflammatory process, faster initial recovery of wounded area, and improved extracellular matrix deposition. These results indicate that extracted carrageenan hydrogel can serve as a scaffold for in vitro growth and maintenance of human SD-MSCs, being also able to act as a delivery system of cells to wounded skin. Thus, evaluation of the properties discussed in this study contribute to a further understanding and specificities of the potential use of carrageenan hydrogel as a delivery system for several applications, further to skin wound healing.

Laju Pertumbuhan Ikan Kerapu Bebek Cromileptes altivelis yang Dipelihara dalam Keramba Jaring Apung

2016 ◽  
Vol 1 (1) ◽  
pp. 11 ◽  
Author(s):  
Safar Dody ◽  
Dinawanti La Rae
Keyword(s):  
Growth Rate ◽  
Stocking Density ◽  
Mesh Size ◽  
Fish Farming ◽  
Total Body Weight ◽  
Water Clarity ◽  
Trash Fish ◽  
Absolute Growth ◽  
Total Body ◽  
Net Cages

<strong>Growth Rate of Humpback Grouper Cromileptes altivelis Cultured in Floating Net Cages.</strong> Humpback Grouper <em>Cromileptes altivelis</em> fish farming has good opportunities in the future, although still experiencing problems in its cultivation. One of the parameters that needs to be considered is the stocking density associated with the cage area. The purpose of this study was to determine the appropriate stocking density to generate optimum growth of Humpback Grouper cultivated in floating net cages. The study was conducted using the experimental method with 3 treatments and 3 replications. There were 9 cages used with the size of 1 x 1 x 1.5 m3 and a mesh size of 0.5 inches. The study was conducted in the waters of Banda Neira, Maluku, with the condition of the waters during the experiment, such as salinity which ranged from 33.2 to 34.6‰, water temperature ranged from 25 to 31°C, pH ranged from 7 to7.5, current speed 0.32–3.97cm/s, and water clarity was 14.29 to 20 m. The feed was trash fish given twice a day, in the morning and afternoon as much as 10% of total body weight of fish. After 4 months of cultivation, the absolute growth was best demonstrated by the treatment with stocking density of 25 fish/cage, which was 18.22 g, while the lowest (13.25 g) was shown by the treatment with stocking density of 75 fish/cage. The specific growth rate in the treatment of 25 fish/cage reached 1.34%/day, while the treatment of 50 fish/cage and 75 fish/cage reached 1.18%/day and 0.97%/day respectively.<br /><br /><br />

Growth rate of the carrageenophyte Kappaphycus alvarezii (Rhodophyta, Gigartinales) in vitro

2001 ◽  
Vol 49 (3) ◽  
pp. 155-161 ◽  
Author(s):  
Edison Jose De Paula ◽  
Cintia Erbert ◽  
Ricardo Toledo Lima Pereira
Keyword(s):  
Growth Rate ◽  
Kappaphycus Alvarezii
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