scholarly journals The role of betel (Piper betle) leaf extract and glycerol on physical properties of bioplastic based on sago starch

2021 ◽  
Vol 912 (1) ◽  
pp. 012042
Author(s):  
H Nasution ◽  
E Julianti ◽  
N F Dalimunthe ◽  
G Wulandari
Keyword(s):  
Antimicrobial Agent ◽  
Physical Properties ◽  
Leaf Extract ◽  
Piper Betle ◽  
Sago Starch ◽  
Natural Antimicrobial ◽  
Betel Leaf ◽  
Potential Sources ◽  
Absorption Percentage

Abstract Starch-based bioplastic from sago is one of the potential sources in Indonesia. Since it is made from natural ingredients, it is easier to absorb water and susceptible to the activity of microorganisms. Betel leaf extract is one of natural antimicrobial agent that rich of tannin and flavonoid. This study aims to investigate physical properties and antimicrobial activity of sago (Metroxylon sp.) starch-based bioplastic. The betel leaf was extracted by using the ultrasonication method with ethanol as a solvent. Betel leaf concentrated extract is diluted using aquadest with a concentration of 2 g/100 mL (2%) to be applied as an antimicrobial agent in bioplastics. The compositions of glycerol were varied from 10-30% whereas betel leaf extract from 3-9%(v) in the manufacture of bioplastics. The results show that the highest density of 1.5 g/cm3 was achieved at the composition of glycerol and betel leaf extract, respectively, 30%(v) and 9%(v). The highest water absorption percentage was shown by sample with 30%(v) glycerol without addition of betel leaf extract, that was 83.31%. The antibacterial activity showed that the addition of betel leaf extract to bioplastics was able to inhibit the activity of bacteria (Bacillus cereus). The best inhibition were shown in bioplastics with a variation of 20%(v) glycerol and 9%(v) betel leaf extract addition. However, all variation did not show their inhibition with fungal activity (Aspergillus niger).

Efektifitas Ekstrak Daun Sirih Hijau (Piper Betle) sebagai Insektisida Nabati terhadap Mortalitas Lalat Rumah (Musca domestica)

2019 ◽  
Vol 10 (2) ◽  
pp. 44-50
Author(s):  
Rinaldi Daswito ◽  
Rima Folentia ◽  
M Yusuf MF
Keyword(s):  
Musca Domestica ◽  
Leaf Extract ◽  
Effective Concentration ◽  
P Value ◽  
Piper Betle ◽  
Microbiology Laboratory ◽  
House Flies ◽  
Betel Leaf ◽  
The One ◽  
One Way Anova

One of the diseases that can be transmitted by flies is diarrhea. Green betel leaf contains essential oils, chavicol, arecoline, phenol, and tannins which function as plant-based insecticides. This study aimed to determine the effectiveness of green betel leaf extract (Piper betel) as a plant-based insecticide on the number of mortality of house flies (Musca domestica). The research was an experimental study used After Only Design used the One Way Anova test with a 95% confidence level. The samples used were 360 ​​house flies. Each treatment of 30 house flies with 4 repetitions and used three concentrations of green betel leaf extract (25%, 50%, 75%). The study was conducted at the Chemistry and Microbiology Laboratory of Health Polytechnic Tanjungpinang, while the location of the fly collection was at the Tokojo Garbage Collection Station in Bintan Regency. The number of mortality of house flies at a concentration of 25% was 81 heads (67.5%), 50% concentrations were 93 heads (77.5%), and at a concentration of 75% were 103 heads (85.83%). There was an effect of green betel leaf extract on the mortality of house flies (p-value 0.0001 <0.05) with the most effective concentration of 75%. Further research is needed to obtain a finished product utilizing green betel leaf extract as a vegetable insecticide, especially in controlling the fly vector. Need further research on the use of green betel leaf extract as a vegetable insecticide controlling the fly vector by taking into account the amount of spraying and the age of the fly.   Keywords: Green betel leaf extract , organic insecticide, houseflies

scholarly journals Analisis Daya Hambat Ekstrak Daun Sirih Hijau (Piper betle L.) terhadap Mikroorganisme Indikator Mastitis

2020 ◽  
Vol 4 (2) ◽  
pp. 230-236
Author(s):  
Dicky Rizaldi Rambe ◽  
Allaily Allaily ◽  
Cut Intan Novita
Keyword(s):  
Leaf Extract ◽  
Agricultural Product ◽  
Piper Betle ◽  
Product Analysis ◽  
E Coli ◽  
Betel Leaf ◽  
Randomized Design ◽  
Inhibitory Analysis ◽  
Analysis Laboratory ◽  
Range Test

Abstrak. Penelitian tentang Analisis Daya Hambat Ekstrak Daun Sirih Hijau (Piper betle L.) terhadap Mikroorganisme Indikator Mastitis telah dilaksanakan di Laboratorium Ilmu dan Teknologi Pengolahan Susu dan Laboratorium Analisis Pangan dan Hasil Pertanian yang dilaksanakan pada 7 September sampai dengan 10 November 2018. Penelitian ini terdiri dari 4 perlakuan dan 5 ulangan. Data yang diperoleh selanjutnya akan diolah menggunakan Rancangan Acak Lengkap, jika didapatkan hasil yang berbeda maka akan dilanjutkan dengan uji Duncan. Perlakuan R1 yaitu penambahan ekstrak daun sirih hijau 15%, R2 yaitu penambahan ekstrak daun sirih hijau 30%, R3 yaitu penambahan ekstrak daun sirih hijau 45% dan R4 yaitu penambahan ekstrak daun sirih hijau 60%. Hasil penelitian menunjukkan bahwa ekstrak daun sirih hijau dengan konsentrasi 15%, 30%, 45% dan 60% mampu menghambat E. coli dan S. aureus. Berdasarkanhasil penelitian ini dapat disimpulkan bahwa ekstrak daun sirih hijau mampu menghambat pertumbuhan E. coli dan S. aureus.Inhibiting Power Analysis of Green Betel Leaf Extract (Piper betle L.) against Microorganism Indicator MastitisAbstract. Research on Inhibitory Analysis of Green Betel Leaf Extract (Piper betle L.) against Mastitis causing Microorganism has been conducted in Milk Processing Science and Technology Laboratory and  Food and Agricultural Product Analysis Laboratory held on September 7 to November 10, 2018. This research consisted of 4 treatments and 5 replications. The data obtained will then be processed using a Completely Randomized Design, if different results are obtained it will be followed by the Duncan Multiple Range Test. The treatment of R1 is the addition of 15% green betel leaf extract, R2, which is the addition of 30% green betel leaf extract, R3, namely the addition of 45% green betel leaf extract and R4 which is the addition of 60% green betel leaf extract. The results showed that green betel leaf extract with a concentration of 15%, 30%, 45% and 60% was able to inhibit E. coli and S. aureus. Based on the results of this study it can be concluded that green betel leaf extract can inhibit the growth of E. coli and S. aureus.

scholarly journals Efektivitas Ekstrak Daun Sirih (Piper betle L) dan Ekstrak Bawang Putih (Allium sativum) terhadap Mortalitas Larva Nyamuk Aedes aegypti

bionature ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Amirullah Amirullah ◽  
Nurhayu Malik ◽  
Rosmaya Rosmaya
Keyword(s):  
Aedes Aegypti ◽  
Endemic Area ◽  
Leaf Extract ◽  
Allium Sativum ◽  
Larval Mortality ◽  
Hemorrhagic Fever ◽  
Garlic Extract ◽  
Piper Betle ◽  
Mosquito Larvae ◽  
Betel Leaf

Abstract. The study of the efficacy of betel leaf extract (Piper betle L.) and garlic extract (Allium sativum) on the mortality of Aedes aegypti mosquito larvae was carried out at the Zoology Laboratory of the Faculty of Mathematics and Natural Sciences, Halu Oleo Kendari University. Aedes aegypti mosquito larvae were obtained from the rearing of F2 from Aedes aegypti eggs that were taken by ovitrap from in Kambu Village, Kendari City, which is an endemic area of Dengue Hemorrhagic Fever (DHF). Extrac of betel leaf (Piper betle L.) and garlic extract (Allium sativum) at concentrations of 1.0%, 2.0% and 3.0% respectively and their combinations were given to Aedes aegypti larvae (L4). The results showed that the highest larval mortality (88%) occurred after 24 hours of exposure to betel leaf extract (Piper betle L.) at a concentration of 3%, whereas with garlic extract (Allium sativum) the highest larval mortality (98%) occurred at concentration 3 %, and for the highest larval mortality (99%) found in a combination of 3% betel leaf extract (Piper betle L.) and 3% garlic extract (Allium sativum). Based on WHO (2009), the most effective treatment for killing Aedes aegyti larvae is 3% garlic (Allium sativum) extract and a combination of 3% betel leaf extract (Piper betle L.) and 3% garlic extract (Allium sativum).Keywords:  Biopesticide, Mortality, Aedes aegypti, betle leaf (Piper betle L.), garlic (Allium sativum).

scholarly journals IDENTIFICATION OF ANTHRACHNOSE DISEASE ON STRAWBERRY FRUIT (Fragraria vesca L.) AND ITS CONTROL BY BETEL (Piper betle L.) LEAF EXTRACT

2015 ◽  
Vol 2 (1) ◽  
pp. 458
Author(s):  
Rina Sri Kasiamdari ◽  
Umi Sangadah
Keyword(s):  
Disease Severity ◽  
Leaf Extract ◽  
Piper Betle ◽  
Strawberry Fruit ◽  
Pathogen Infection ◽  
Anthracnose Disease ◽  
Betel Leaf ◽  
Colletotrichum Spp

<p>In Indonesia, strawberry is one of fruits which has high economic values. The growth of strawberry has increased significantly, but its productivity is still low because of disease attack. Disease that attacks strawberry started from nursery to post harvest is anthracnose that can be caused by Colletotrichum spp. Control efforts of anthracnose disease up to now has been done with application of synthetic chemicals which are known to be hazardous to the environment and human health. Betel leaf (Piper betle L.) is natural plant that potentially used as phytofungicide. Essential oils on betel leaf extract is reported can decrease the growth of pathogenic fungi. The objectives of the research were to identify Colletotrichum spp. in strawberry fruit that showed anthracnose symptoms, to test the potency of betel leaf extract as phytofungicide, and to evaluate the effect of betel leaf extract in inhibiting infection and disease severity of Colletotrichum in strawberry. Samples were taken from strawberry nursery in Magelang, Indonesia. The disease symptoms were isolated on Potato Dextrose Agar, followed by identification of fungal colony and morphology by semi permanent slide preparation. Betel leaves were extracted with water to get a concentration of 20, 40, 60, 80 and 100%, respectively. An in vitro experiment was done to evaluate the inhibition rate of betel leaf extract to the growth of Colletotrichum spp. colony. An in vivo experiment was done by submersion of strawberry to betel leaf extract before (preventive method) and after antrachnose attack (curative method), then disease infection and disease severity were measured after 7 days. The result of the research showed that anthracnose disease in strawberry was caused by C. gloeosporioides (Penz.). Twenty percent of betel leaf extract in an in vitro test had potential as phytofungicide and concentration of 100% betel leaf extract was the best concentration to inhibit the growth of C. gloeosporioides (Penz.) colony by 70.26±0,61%. In an in vivo experiment, submersion of strawberry before pathogen infection (preventive) with 100% betel leaf extract decreased disease infection by 40% and disease severity by 16%, respectively. While treatment of submersion of strawberry after pathogen infection (curative) decreased disease infection by 33,4% and disease severity by 17%. </p><p>Keywords: strawberry, anthrachnose, disease, betel leaf</p>

scholarly journals Preparation and Characterization of Betel Leaves (Piper betle Linn) Extract Nanoparticle with Ionic Gelation Method

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Dwi Saryanti ◽  
Dian Nugraheni ◽  
Nisa Sindi Astuti
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Leaf Extract ◽  
Particle Morphology ◽  
Particle Size Analysis ◽  
Size Analysis ◽  
Piper Betle ◽  
Ionic Gelation ◽  
Betel Leaf

Nanoparticles are used in drug delivery which can increase mass transfer so increase the absorption and effectiveness of the drug. Therefore, its prospect to improve antibacterial and antioxidants activities of betel leaves. The research aimed to preparation and characterization of betel leaf extract using ionic gelation technique. The formulation of nanoparticles from betel leaf extract with ionic gelation method using alginate and CaCl2 with a ratio of 2.5: 1. The characterization of the nanoparticles includes particle size analysis, zeta potential, particle morphology and determination of flavonoid content. Particle size analysis demonstrated that the betel leaf extract nanoparticles had a particle size of 243,03 ± 1,48 nm, zeta potential of -23,0 ± 0,35 mV and morphology of particle showed that a flat shape. The betle leaf exctract nanoparticle positively contained flavonoid with Rf 0.7 equivalent to quercetin. The betel leaf extract can be made nanoparticles with ionic gelation method using alginate and CaCl2.

scholarly journals THE PERCENTAGE OF CARCASS PARTS OF MEAL LOCAL DUCKS GIVEN BETEL LEAF SOLUTION INCLUDED IN RATIO

2017 ◽  
Vol 3 (1) ◽  
pp. 41
Author(s):  
Maria Sale ◽  
Ristika Handarini ◽  
Elis Dihansih
Keyword(s):  
Leaf Extract ◽  
Animal Husbandry ◽  
Piper Betle ◽  
Betel Leaf ◽  
Extract Solution ◽  
Randomized Design ◽  
Commercial Feed ◽  
Period Data ◽  
Test Result ◽  
Average Body

This study was aimed at assessing the effects of inclusion of betel leaf extract solution in ration on the percentage of carcass parts of male local ducks. The study was conducted at the Poulty Farm of department of Animal Husbandry, Faculty of Agriculture, Djuanda University, Bogor from June to August 2016. Twenty-four male local ducks aged 2 weeks with average body weight of 450 g were used. The ducks were alocated into battery pens (150 cm x 40 cm) and fed BR-21E ration and betel leaf extract solution. Drinking water was given ad libitum. A completly randomized design with 4 treatments and 3 replicates was used. Treatments consisted of 100% comercial feed (R1) , comercial feed +2.5% piper betel solution (R2), commercial feed+5.0% piper betel solution (R3), and commercial feed + 7,5% piper betel solution (R4). Treatments were given at the second week following the adaption period. Data were subjected to an analysis of variance and a Duncan test. Result showed that the inclusion of betel leaf extract of 2.5, 5, 7.5% in the ration gave significant effects (P<0.05) on the percentage of carcass, wing, and tigh meat but not (P>0,05) on the percentage of back, breast and its parts, thigh, skin and femur. It was recommended that the inclusion of betel leaf extract in the ration be done by 5% in order to obtain high percentage of and more economical carcass, wing and thigh.Keywords : percentage of carcass part, male local duck, piper betle linn leaf solution

scholarly journals Uji Aktivitas Antifungi Ekstrak Daun Sirih (Piper betle L) Terhadap Saprolegnia sp. Secara In Vitro [Antifungal Activities Test Of Betel Leaf Extract (Piper betle L) On Saprolegnia sp. By In Vitro]

2019 ◽  
Vol 5 (1) ◽  
pp. 15
Author(s):  
Rahayu Kusdarwati, Pustika Murtinintias, Dewa Ketut Meles
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Piper Betle ◽  
Minimum Concentration ◽  
Minimum Fungicidal Concentration ◽  
Betel Leaf ◽  
Concentration Test ◽  
Fungal Suspension

Abstract Saprolegniasis is a mycotic disease caused by Saprolegnia sp. that usually attacking wild fish and farming fish. Saprolegnia sp. cause a lot of harm in process of the fish cultivation. Prevention and treatment of the common practice is use chemical drugs, but the use of these chemicals tend to be environmentally unfriendly and there are has karsinogenik effect. Therefore, the use of traditional medicines is one of alternative to control Saprolegnia sp. safer than chemical drugs. Green betel leaf contains phenolic compounds and tannins are efficacious as antifungal agent. This study aims to prove the antifungal activity of extracts of betel leaf (Piper betle L) for Saprolegnia sp., and to know the minimum concentration of betel leaf extract (Piper betle L) as antifungal for Saprolegnia sp. The design of this experiment is used completely randomized design (CRD) with 11 treatments and 3 replications. This study used the dilution method through the Minimum Inhibitory Concentration determination (MIC) and Minimum Fungicidal Concentration (MFC). The concentration of the extract used was 50% (0.5 g/ml), 25% (0.25 g/ml), 12.5% (0.125 g/ml), 6.25% (0.0625 g/ml), 3.13% (0.0313 g/ml), 1.56% (0.0156 g/ml), 0.78% (0.0078 g/ml), 0.39% (0.0039 g/ml), 0.2% (0.002 g/ml) of betel leaf extract. A positive control containing 2 ml of 3% hydrogen peroxide were added fungal suspension until 4 ml Negative control containing 2 ml of 10% DMSO were added fungal suspension until 4 ml. The main parameters in this study is the value of optical density (OD) for MIC (Minimum Inhibitory Concentration) test and the absence of Saprolegnia sp. growing on SDA media for MFC (Minimum Fungicidal Concentration) test. 0.20% (0.002g/ml) concentration of of betel leaf extract is the minimum concentration that can inhibit the growth of Saprolegnia sp. MFC test results showed concentrations of 0.78% (0.0078 g/ml) betel leaf extract is the minimum concentration that can kill Saprolegnia sp.

Antimicrobial Effect of Piper betle Leaves Extract on Cotton Fabrics for Vaginal Discharge Sanitary Napkins

2021 ◽  
Vol 1162 ◽  
pp. 159-165
Author(s):  
Srie Gustiani ◽  
Wulan Septiani ◽  
Cica Kasipah ◽  
Danny Sukardan ◽  
Qomarudin Helmy
Keyword(s):  
Cotton Fabric ◽  
Lactobacillus Acidophilus ◽  
Leaf Extract ◽  
Vaginal Discharge ◽  
Antimicrobial Property ◽  
Antimicrobial Effect ◽  
Cotton Fabrics ◽  
Piper Betle ◽  
Excellent Performance ◽  
Betel Leaf

The betel (Piper betle) leaves have been using for traditional medicine in several tropical countries, like Indonesia. The betel leaves have the antimicrobial property for inhibiting the growth of microbes. In this study, cotton fabric was treated with the betel leaves extract 1, 5 and 10 g/l. The peper betel leaves are extracted by using boiling water up to half. The betel leaf extract was imparted onto the cotton fabrics for underclothes apparel using a pad-dry-cure. The antimicrobial activity property of treat cotton fabrics was assessed (Escherchia coli, Staphylococcus aureus, Lactobacillus acidophilus, Candida albicans AATCC 100-2004). The piper betle extract more effective to inhibit the growth of Candida Albicans than Escherchia coli, Staphylococcus aureus. Lactobacillus acidophilus as a good bacteria around the vagina, can still grow. From the result of this study, betel leaf is an antiseptic, then can be used in preventing vaginal discharge. The durability of the betel leaf extract on the cotton fabric did not decrease significantly after washing 15 times using Lounder O’meter machine washing. The excellent performance of antimicrobial on the fabrics can keep clean and can help the vaginal area keep clean, odorless, and is expected to prevent vaginal discharge.

Effects of Piper betle Leaf Extract on Biofilm and Rhamnolipid Formation of Pseudomonas aeruginosa

2021 ◽  
pp. 5182-5186
Author(s):  
Irene Ratridewi ◽  
Shod A. Dzulkarnain ◽  
Andreas B. Wijaya ◽  
John T. R. Huwae ◽  
Daniel S. M. Putra ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Linear Regression Analysis ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Piper Betle ◽  
Betel Leaf ◽  
Polystyrene Plate ◽  
Beta Coefficient ◽  
Agar Dilution ◽  
Adherence Test

High mortality rate and antimicrobial resistance are still becoming world-wide problems, due to Pseudomonas aeruginosa’s (P. aeruginosa) virulence and its ability to form biofilm. Biofilm’s formation is affected by the presence of rhamnolipid, whose production is regulated by quorum sensing systems. Piper betle (P. betle) possesses antimicrobial, antioxidant, anti-inflammatory and immunomodulatory properties. The aim of our study is to investigate the effects of P. betle leaf’s extract against biofilm formation and rhamnolipid production of P. aeruginosa. Active compounds of P. betle were identified using plate chromatography. Agar dilution method was used to determine the minimum biofilm inhibitory concentration (MBIC) of methanolic leaf extract of P. betle. A biofilm-producing P. aeruginosa isolate in the polystyrene plate adherence test was selected for confirmation of biofilm production by Scanning Electron Microscopy (SEM), after P. betle administration. Rhamnolipid detection and evaluation were performed by interpreting halo formed around the well. After administration of various concentrations of P. betle leaf extract on the microplate well, it was concluded that the MBIC of P. betle leaf extract on P. aeruginosa was 0.4%. Methanolic extract of P. betle leaf extract at concentration of 0.4% showed that P. aeruginosa could not form biofilm at all, although the bacteria could still aggregate and form a matrix. After linear regression analysis, beta-coefficient was obtained at -0.931 for P. betle leaf extract. It can be concluded that P. betle leaf extract was effective in inhibiting the growth of biofilm and formation of rhamnolipid by P. aeruginosa. The increase in concentration of P. betle leaf extract was inversely proportional to the diameter of the halo rhamnolipid formed. The higher the level of P. betel leaf extract, the smaller the diameter of the halo rhamnolipid formed.

Sign in / Sign up

Export Citation Format

Share Document