scholarly journals Measurement of macroalgae total carbohydrate content found in Lendang Luar coast, Lombok, Indonesia for potential sources of bioethanol

2021 ◽  
Vol 913 (1) ◽  
pp. 012077
Author(s):  
B T K Ilhami ◽  
A S Abidin ◽  
N W R Martyasari ◽  
N S H Kurniawan ◽  
H Padmi ◽  
...  

Abstract Bioethanol is a renewable alternative energy through the process of fermenting sugar from carbohydrate sources by adding microorganisms such as yeast or bacteria. Macroalgae or known as seaweed is one of the producers in the marine environment which has a high carbohydrate content so that it can be used as raw material for bioethanol. Macroalgae contains specific carbohydrates including laminarin, mannitol, alginate, agar and polysaccharides. The high carbohydrate content indicates the ethanol content produced. The aim of the study was to initial screen total carbohydrates in red algae (G. latifolium and G rugosa) and brown algae (M rosea, S. crassifolium, S. cristaefolium, S. polycystum, P.australis and T. muray ana). Carbohydrate total analysis was performed by colorimetric assay using the BioVision kit. The overall carbohydrate content found in macroalgae samples in this study was 28.23 fig (DW)1. The highest carbohydrate content was obtained by G. latifolium (37.50 fig (DW)1), followed by G rugosa (34.27 fig (DW)1) and S. cristaefolium (33.33 fig (DW)1). Current results show that macroalgae exhibits sufficient amounts of carbohydrate which could potentially be further developed as source for biotehanol.

Author(s):  
Laura Arystan ◽  
◽  
Tolkyn Kamsayeva ◽  
Saltanat Tleukenova ◽  
Denis Sirman ◽  
...  

This article is devoted to the study of the influence of cryopreservation on the preservation of sowing qualities of seeds of representatives of the genus Picea: P. asperata (Rough spruce) and P. pungens (Spiny spruce). As an alternative shock method of cryopreservation, the method of step-by-step 3-step temperature reduction was used 1 stage +4 °C, 2 stage –18 °C, 3 stage –196 °C liquid nitrogen. Additionally, the effect of 4 time intervals (1 hour, 24 hours, 72 hours and 168 hours) of step freezing on seed quality was investigated. To explain the results by photometry, the total carbohydrate content of the seeds is determined before the 3rd freezing step (cryo interaction). The analysis of the results of germination showed that the seeds of the studied species respond in different directions to the step cryo freezing. So, seeds of P. asperata germination in all tree experimental variants are higher on 4–32 % than in the control variant; and the maximum results are revealed in the variant with time intervals of 168 hours. For P. pungens seeds, the germination rates in experimental variants were on 18–22 % lower than in the control; and the largest germination rate of seeds among experimental variations are revealed in variant with interval 24 hours. The determination of the total carbohydrate content of the tested seeds at different cryogenic freezing intervals found that this indicator varied significantly depending on the time interval. The lowest values of total carbohydrates are found in variation with a time interval — 24 hours. The maximum total carbohydrate content is determined in a variation with a time interval — 1 hour. Correlation analysis between seed germination indices and total carbohydrate content established a high relationship between these indices. So, for P. asperata, the value of the Pearson coefficient was 0.86, and for P. pungens seeds — 0.91.


2013 ◽  
Vol 41 (1) ◽  
pp. 255 ◽  
Author(s):  
Ercan YILDIZ ◽  
Mustafa KAPLANKIRAN ◽  
Turan Hakan DEMIRKESER ◽  
Celil TOPLU

The study was conducted at the experimental farm of Mustafa Kemal University, Dörtyol, Turkey during the 2010 and 2011 growing seasons. The aim of this study was to investigate the effect of ‘Troyer’ and ‘Carrizo’ citranges (Poncirus trifoliata Raf. × Citrus sinensis Osb. var. ‘Troyer’ and ‘Carrizo’), and common sour orange (Citrus aurantium L. var. common), rootstocks on the seasonal variation of carbohydrate content in the leaves of cvs. ‘Fremont’, ‘Nova’, and ‘Robinson’ mandarin. The seasonal variation of carbohydrate content of the three cultivars budded on different rootstocks was nearly same. Soluble carbohydrate concentration showed a continuous decrease from January to mid or late-summer, and then slowly began to increase after early autumn till winter. The sucrose was the dominant soluble carbohydrate in leaves. The seasonal evolution of starch content in leaves increased initially during January to March, and then decreased in April. The starch concentration showed a continuous decrease slowly until the mid-autumn, and then accumulation began during late-autumn and winter. The total carbohydrate content differences among the rootstocks were significant, but the content was changed among the cultivars and according to the season. The change in the total carbohydrate content of leaf tissues showed a strong similarity in cultivars budded on different rootstocks throughout the year. The total carbohydrate content reached their lowest levels in July for cv. ‘Robinson’, in August for cv. ‘Fremont’ and cv. ‘Nova’. The total carbohydrate content in leaves increased from the mid- or late-summer to winter. It is suggested that the seasonal variation of carbohydrate content in plant tissues can be considered during fertilization program in mandarin trees.


2018 ◽  
Vol 64 (No. 9) ◽  
pp. 379-386 ◽  
Author(s):  
Deligöz Ayşe ◽  
Bayar Esra ◽  
Genç Musa ◽  
Karatepe Yasin ◽  
Kirdar Erol ◽  
...  

Variations in the photosynthetic pigments and total carbohydrate contents of needles of different age classes (current-year, 1-year-old, 2-year-old and 3-year-old) of Pinus nigra subsp. pallasiana (Lambert) Holmboe trees in a young natural stand were investigated during the growing season. In current-year needles, total carbohydrate content was lower during June and July when the needle growth continued than in older age classes, but it was similar to other age classes in the months of August to October. Seasonal patterns of variations in total carbohydrate content were almost similar in 1-, 2-, and 3-year-old needles. Chlorophyll and carotenoid contents increased from May to June, remained relatively constant or declined slightly during summer and autumn in 1-, 2-, and 3-year-old needles. In October, the pigment content was highest in 1-year-old needles, and lowest in 3-year-old needles. Our study indicated that total carbohydrate and pigment contents were affected by needle age classes and seasons.


HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 899F-900
Author(s):  
Moo R. Huh ◽  
Beyoung H. Kwack ◽  
Leonard P. Perry

Salt injury was induced by 5% (w/v) NaCl drenching on Hibiscus hamabo Sieb. & Zucc. and H. syriacus L. seedlings. Total chlorophyll content of H. hamabo was higher than that of H. syriacus. Uniconazole (0.0, 0.5, 1.0, and 5.0 mg·liter–1) treatment increased and 25- or 50-mg·liter–1 GA3 treatment decreased chlorophyll content of H. hamabo. Total chlorophyll content of H. syriacus was not affected by uniconazole or GA3. Total carbohydrate content of H. syriacus was more accumulated than that of H. homabo. Total carbohydrate content of H. hamabo was more decreased than that of H. syriacus by Ca (13.35 or 133.5 mM), uniconazole, or GA3 in relation to total carbohydrate contents. Protein contents of H. hamabo were higher than those of H. syriacus. Uniconazole or GA3 increased those of H. hamabo and decreased those of H. syriacus. Peroxidase activity of H. hamabo was higher than that of H. syriacus. Uniconazole decreased that of H. hamabo and increased that of H. syriacus. GA3 or Ca (13.35 mM) treatment increased that of both species. ATPase activity of H. hamabo was higher than that of H. syriacus. Uniconazole (5 mg·liter–1), GA3, or Ca decreased that of H. hamabo increased that of H. syriacus.


2019 ◽  
Vol 9 (2) ◽  
Author(s):  
M.M Jayakody ◽  
M.P.G Vanniarachchy ◽  
I. Wijesekara

Seaweeds are a rich source of health beneficial bioactive nutraceuticals and currently they are under-utilized in Sri Lanka. In the present study, proximate analysis of seaweed varieties Chnoospora minima and Porphyra sp. obtained from Mirissa, Matara, Sri Lanka and Ulva fasciata was taken from Point Dondra Matara, Sri Lanka on June, 2018 were investigated. The moisture content, total fat content, protein content and ash content were determined according to the Official methods of Analysis by Association of Official Analytical Chemists after drying for 8h at 600 C. The results revealed that the moisture contents (%) of Chnoospora minima, Porphyra sp. and Ulva fasciata were 13.24 ± 0.20, 14.30 ± 0.14 and 18.11 ± 0.01 respectively. Total fat contents (%) of Chnoospora minima, Porphyra sp. and Ulva fasciata were 0.21 ± 0.11, 0.19 ± 0.03 and 0.28 ± 0.05 respectively. Protein contents (%) of Chnoospora minima, Porphyra sp. and Ulva fasciata were 13.70 ± 0.2, 21.14 ± 0.04 and 11.84 ± 0.1. Total ash contents (%) of Chnoospora minima, Porphyra sp. and Ulva fasciata were 17.20 ± 0.24, 5.40 ± 0.7 and 18.05 ± 0.21 respectively. Total carbohydrate content (%) was analyzed according to the Dubois method. Chnoospora minima, Porphyra sp. and Ulva fasciata showed total carbohydrate content (%) as 3.87 + 0.66, 20.59 ± 0.24 and 7.68 ± 1.16 respectively. Moreover, the sulphate content was analyzed according to the precipitate method. Chnoospora minima, Porphyra sp. and Ulva fasciata showed 1.45 ± 0.35, 2.75 ± 0.07 and 4.54 ± 0.06, sulfate contents (%) respectively. In conclusion, highest ash content which indicates a good mineral content was observed in Ulva fasciata and Chnoospora minima. Fibre, protein and carbohydrate contents are significantly different among the 3 samples. Highest fibre content was observed in Chnoospora minima. Highest protein and carbohydrate contents were observed in Porphyra sp. But there is no significant difference in fat contents among the three samples.


1987 ◽  
Vol 67 (3) ◽  
pp. 489-499 ◽  
Author(s):  
J. A. BALDOCK ◽  
B. D. KAY ◽  
M. SCHNITZER

The influence of three cropping treatments on the quantitative and qualitative composition of the carbohydrate fraction of a silt loam soil and its water-stable aggregates was assessed. Three cropping treatments were considered: bromegrass (Bromus inermis Leyss.) grown continuously for 15 yr (B15), grain-corn (Zea mays L.) grown continuously using conventional tillage for 15 yr (C15), and grain-corn grown continuously for 13 yr followed by 2 yr of bromegrass (B13C2). Six water-stable aggregate size fractions ranging from 8.00–4.70 to 0.25–0.10 mm in diameter were separated from the soil by wet sieving. The carbohydrate fraction was extracted by acid hydrolysis, reduced, acetylated to alditol acetates and analyzed by temperature-programmed capillary gas chromatography. Total carbohydrate content was not significantly influenced by cropping treatment but was observed to increase as aggregate size decreased irrespective of cropping treatment. The carbohydrate content in the different aggregate size fractions was positively correlated with both organic carbon and clay contents. With the exception of xylose and mannose, the monomeric sugars behaved similarly to total carbohydrates. Based on the magnitude of the ratio of the content of mannose to that of arabinose plus xylose, the contribution of plant carbohydrates to the total carbohydrate fraction increased as aggregate size decreased in the B15 treatment, but decreased in the C13B2 and C15 treatments. No significant correlations existed between either the total carbohydrate content or the content of individual sugars, and the stability of aggregates in the soils from the different cropping treatments. Key words: Wet aggregate stability, soil organic matter, soil structure, crop rotation, corn, bromegrass


2020 ◽  
Vol 5 (3) ◽  
Author(s):  
Novirina Hendrasarie ◽  
Dimas Eka Mahendra

Vegetables that do not sell and rotten will be thrown away. This makes the volume of waste increases every day. Vegetable waste that is no longer being sold, could reprocessed into useful products and selling value. The purpose of this research is to use vegetable waste to become bioethanol raw material, which have economic value. The selected vegetable waste was from potatoes, carrots, cabbage, cassava, chicory, and green mustard, because they have higher carbohydrate content than other vegetable waste. The bioethanol product produced will be measured by its bioethanol content and fermentation time. These two factors, affect the quality of the bioethanol produced. The microorganism used in this study was Saccharomyces cerevisiae. These microorganisms are in bread yeast and tape yeast, which are also examined for their effectiveness in the production of this vegetable waste bioethanol. From this study, producing bioethanol with the highest ethanol content of 15% v / v, produced in the interaction of bread yeast and 6 days’ fermentation time. Meanwhile, from the production using yeast tape, obtained ethanol levels of 13% v / v. Bioethanol from vegetable waste is not only to reduce the burden of waste generation, but can be used as an alternative energy to replace fuel.


1971 ◽  
Vol 49 (7) ◽  
pp. 1219-1223 ◽  
Author(s):  
George S. Puritch ◽  
M. Talmon-De L'Armee

Effects of balsam woolly aphid infestation on the concentration of ethanol-soluble and -insoluble carbohydrates were investigated in four age classes of needles and six age classes of twigs of grand fir, before and after bud break.In March, before bud break, sugars made up about two-thirds of the total carbohydrate content of the foliage. Aphid infestation did not affect the concentration of sugars in needles but reduced starch by 28%. In twigs, sugar concentrations were less in infested trees while starch concentrations were unaffected. Both older needles and twigs contained higher concentrations of starch than younger needles and twigs.After bud break in June, starch constituted the major form of carbohydrate, accounting for 65% of the total carbohydrate in non-infested foliage and 53% in infested foliage. Total carbohydrate content increased over that in March, in both infested and non-infested foliage, but the non-infested tissue showed the greatest increase. Starch content of infested foliage was reduced by 36%, while starch concentration of infested twigs was reduced by 18%.


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