scholarly journals The accuracy of several in vitro methods in estimating in vivo digestibility of the tropical dairy ration

2022 ◽  
Vol 951 (1) ◽  
pp. 012012
Author(s):  
Despal ◽  
D T P Manik ◽  
D Evvyernie ◽  
R Zahera
Keyword(s):  
Dry Matter ◽  
Ph Value ◽  
In Vitro Digestibility ◽  
In Vitro Methods ◽  
Ph Values ◽  
Dry Matter Digestibility ◽  
Local Setting ◽  
Randomized Design

Abstract In vitro digestibility methods have been developed to overcome problems in the in vivo digestibility measurement, but its accuracy should be tested in a local setting. In vitro methods developed by Tilley and Terry (T2), Theodorou (T3) and Sutardi (T4) have been compared to in vivo method (T1) in a block randomized design study. Four heifers FH (337.50 ± 45.87 kg BW) were used in T1, and two fistulated FH bulls (510 ± 20 kg BW) were used as inoculant sources in the in vitro methods. Dairy cattle ration consisted of 54.0% Napier grass and 46.0% concentrate with 58.8% DM, 12.1% ash, 10.0% CP, 3.3% EE, 26.5% CF, and 61.1% TDN. The observed parameters were ration fermentability (pH, NH3, and VFA concentration) and digestibility (DMD and OMD). The data were analyzed using analysis of variance (ANOVA) followed by the Tukey test. The correlation was made before regression analysis to estimate the in vivo parameters from the in vitro. The results showed that pH values are in the normal range (6.7 – 6.8), and insignificantly different between treatments (P>0.05). The concentration of NH3 and VFA were significantly different between the treatments (P<0.05), but T2 produced similar NH3 and VFA concentrations to T1. Similar results were also found in the DMD and OMD. Correlation analysis showed that pH value of T3 correlated significantly with T1, while DMD value of T4 correlated to T1. The T1 DMD (Y) could be estimated from T4 DMD (X) using formula Y (%) = y = -0.091x2 + 9.1632x - 168.4. It is concluded that tropical dairy feedstuffs in vitro digestibility using Tilley and Terry’s method produced similar result to in vivo digestibility method, but in vivo dry matter digestibility can be estimated accurately by in vitro dry matter digestibility using Sutardi method.

scholarly journals Comparison of In Vivo and In Vitro Digestibility in Rabbits

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3267
Author(s):  
Sonia Tassone ◽  
Riccardo Fortina ◽  
Sabah Mabrouki ◽  
Yasser Hachana ◽  
Salvatore Barbera
Keyword(s):  
Dry Matter ◽  
Artificial Saliva ◽  
Distal Colon ◽  
Coefficient Of Determination ◽  
In Vitro Digestibility ◽  
High Fiber ◽  
In Vitro Methods ◽  
Dry Matter Digestibility

The apparent dry matter digestibility of diets for rabbits was measured in vivo (ADMDvv) and in vitro with the Ankom DaisyII Incubator. Four diets were tested: low fiber (LF), LF + 5% of pregerminated fenugreek seeds (PGFS) (LF5), LF + 10% PGFS (LF10), and high fiber (HF). For the ADMDvv, feces samples were collected from 56 White New-Zealand × Californian rabbits fed the 4 diets; animals were randomly allocated into 4 groups and housed in individual cages. For the in vitro trial, 3 methods were tested: fecal inoculum (FA) with Kansans State buffer; fecal inoculum with artificial saliva (FB); and multienzyme (ENZ). Fecal inocula were collected at slaughtering from the distal colon of rabbits fed ad libitum the LF diet. For FA and FB methods, the digestibility was measured at 36 and 48 h. The in vitro methods ranked the apparent dry matter digestibility of diets in the same order as in vivo, but ENZ values were always higher than FA and FB at 36 and 48 h. The prediction equations of in vivo digestibility with the ENZ method showed higher coefficient of determination (R2 = 0.69) and lower SE (0.190) than FA and FB; also, reproducibility was higher with ENZ (CV = 3.1%). In conclusion, different methods can be applied to the Ankom DaisyII Incubator to study the digestibility in rabbits. In our trial, the better reproducibility was observed with the multienzyme method than FA and FB were probably related to the variability of inocula.

Differences between the in vitro digestibility of extrusa collected from oesophageal fistulated steers and the forage consumed

2009 ◽  
Vol 49 (7) ◽  
pp. 563 ◽  
Author(s):  
David B. Coates ◽  
Robert J. Mayer
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
In Vitro Digestibility ◽  
In Vitro Techniques ◽  
Pasture Legumes ◽  
Dry Matter Digestibility ◽  
Significant Difference ◽  
C3 Grasses

In a study that included C4 tropical grasses, C3 temperate grasses and C3 pasture legumes, in vitro dry matter digestibility of extrusa, measured as in vitro dry matter loss (IVDML) during incubation, compared with that of the forage consumed, was greater for grass extrusa but not for legume extrusa. The increase in digestibility was not caused by mastication or by the freezing of extrusa samples during storage but by the action of saliva. Comparable increases in IVDML were achieved merely by mixing bovine saliva with ground forage samples. Differences were greater than could be explained by increases due to completely digestible salivary DM. There was no significant difference between animals in relation to the saliva effect on IVDML and, except for some minor differences, similar saliva effects on IVDML were measured using either the pepsin–cellulase or rumen fluid–pepsin in vitro techniques. For both C4 and C3 grasses the magnitude of the differences were inversely related to IVDML of the feed and there was little or no difference between extrusa and feed at high digestibilities (>70%) whereas differences of more than 10 percentage units were measured on low quality grass forages. The data did not suggest that the extrusa or saliva effect on digestibility was different for C3 grasses than for C4 grasses but data on C3 grasses were limited to few species and to high digestibility samples. For legume forages there was no saliva effect when the pepsin–cellulase method was used but there was a small but significant positive effect using the rumen fluid–pepsin method. It was concluded that when samples of extrusa are analysed using in vitro techniques, predicted in vivo digestibility of the feed consumed will often be overestimated, especially for low quality grass diets. The implications of overestimating in vivo digestibility and suggestions for overcoming such errors are discussed.

scholarly journals Pemberian Kulit Biji Kopi dalam Ransum Sapi Aceh Terhadap Kecernaan secara In Vitro

2013 ◽  
Vol 13 (1) ◽  
pp. 49-52
Author(s):  
Yunasri Usman ◽  
M. Nur Husin ◽  
Riantia Ratni
Keyword(s):  
Dry Matter ◽  
Animal Husbandry ◽  
Basal Diet ◽  
Animal Nutrition ◽  
In Vitro Digestibility ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Coffee Beans ◽  
Completely Randomized Design

The supplementation peel coffee beans in the ration Aceh cattle on in vitro digestibilityABSTRACT. This research was carried out at the Laboratory of  Feed and Animal Nutrition,  Department of Animal Husbandry Faculty of Agriculture Syiah Kuala University for  73 days from December 26, 2011 to March 7, 2012. The purpose of this experiment was to find out the effects of the coffee peeling on in vitro digestibility of Aceh beef cattle. Completely Randomized Design (CRD) was applied in this experiment consisting of 4 treatments  namely levels of  coffee peeling on basal diet   (R0=0%, R1=10%, R2=20%, R3=30%). Each treatment consists of 5 replications. Parameters observed in this research were dry and organic matter digestibility. The results showed that dry matter digestibility was not significantly different (P0,05)amongst treatments  54,42%, 49,32%, 45,97%, 42,08%) for R0, R1, R2 and R3 respectively.  On the other hand, organic metter digestibility was significantly different (P0,05) in which R2 treatment was the highest and R0 treatment was the lowest (54,59%).

scholarly journals Evaluasi Kecernaan In Vitro Complete Feed Fermentasi Berbahan Dasar Ampas Sagu dengan Teknik Fermentasi Berbeda

2016 ◽  
Vol 1 (1) ◽  
pp. 808-815
Author(s):  
Sugrahadi Ahmad Aprianto ◽  
Yunasri Usman ◽  
Asril Asril
Keyword(s):  
Organic Matter ◽  
Dairy Cattle ◽  
Dry Matter ◽  
Animal Husbandry ◽  
In Vitro Digestibility ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Organic Matter Digestibility

Abstrak: Penelitian ini telah dilaksanakan di Laboratorium Ilmu Nutrisi dan Makanan Ternak Program Studi Peternakan Fakultas Pertanian Universitas Syiah Kuala dan dilanjutkan dengan pengiriman sampel penelitian ke Laboratorium Ternak Perah Institut Pertanian Bogor (IPB) untuk pengujian kecernaan secara in vitro. Tujuan dari penelitian ini adalah menguji kecernaan secara in vitro complete feed berbahan dasar ampas sagu dengan teknik fermentasi yang berbeda.Pembuatan complete feed disusun sesuai dengan kebutuhan ternak domba. Penelitian ini menggunakan Rancangan Acak lengkap (RAL) dengan 3 perlakuan dan masing-masing perlakuan terdiri atas 5 ulangan. Perlakuan 1 (kontrol) adalah complete feed tanpa penambahan Saus Burger Pakan (SBP) dan tanpa fermentasi, perlakuan 2 adalah ampas sagu difermentasi dengan SBP selama 14 hari kemudian dicampur menjadi complete feed dan difermentasi hingga 21 hari, sedangkan perlakuan 3 adalah ampas sagu + complete feed kemudian difermentasi dengan SBP selama 21 hari. Parameter yang diamati dalam penelitian ini adalah pH, Bahan Kering (BK), Bahan Organik (BO), Koefisien Cerna Bahan Kering (KCBK), dan Koefisien Cerna Bahan Organik (KCBO). Hasil pengamatan menyimpulkan bahwa, teknik fermentasi yang berbeda berpengaruh sangat nyata (P0,01) terhadap semua parameter yang diamati, yaitu pH, BK, BO, KCBK dan KCBO. Dari hasil penelitian dapat disimpulkan bahwa nilai kecernaan complete feed berbahan dasar ampas sagu dipengaruhi oleh perbedaan teknik fermentasi. Digestibility Evaluation In Vitro Complete Feed Fermentation Sago Residues Based with Different Fermentation Techniques Abstract: This research was conducted at the Laboratory of Nutrition and Feed Studies, Program study Animal Husbandry, Faculty of Agriculture, University of Syiah Kuala and continued with the delivery of sample to the Laboratory of Dairy Cattle Institute Pertanian Bogor (IPB) for testing of in vitro digestibility. The purpose of this study was to test in vitro digestibility of complete feed made from  the residues of sago with different fermentation techniques. Complete feed was arranged based on the needs of sheep. This study used a complete randomized design (CRD) with 3 treatments and each treatment consisted of 5 replicates. Treatment 1 (control) was a complete feed without the addition of Saus Burger Pakan (SBP) and without fermentation, treatment 2 was sago residu  fermented with SBP for 14 days and then mixed into a complete feed and fermented up to 21 days, whereas treatment 3 was the sago residue + complete feed then fermented with SBP for 21 days. The parameters observed in this study were pH, Dry Matter (BK), Organic Matter (BO), Dry Matter digestibility coefficients (KCBK), and Organic Matter Digestibility Coefficient (KCBO). The result influence showed that, different fermentation technique was significantly (P 0.01) all parameters, namely pH, BK, BO, KCBK and KCBO. It can be concluded that the digestibility of complete feed made from sago residue influenced by differences in fermentation techniques

scholarly journals Penggunaan Cairan Ekstrak Isi Gizzard dan Duodenum Ayam pada Pengukuran Kecernaan In Vitro Daun Turi (Sesbania grandiflora)

2022 ◽  
Vol 11 (3) ◽  
pp. 225
Author(s):  
Ali Mursyid Wahyu Mulyono ◽  
Sri Sukar Yani ◽  
Jizan Fahmia Al Awanis
Keyword(s):  
Dry Matter ◽  
Protein Digestibility ◽  
Control Treatment ◽  
In Vitro Digestibility ◽  
Dry Matter Digestibility ◽  
Sesbania Grandiflora ◽  
Randomized Design ◽  
Completely Randomized Design

Abstract The aim of this study was to examine the use of Gizzard Contents Extracts (GCE) and Duodenum Contents Extracts (DCE) of Chicken on In Vitro digestibility measurements of Turi Leaves (Sesbania grandiflora). The study was designed using a One-way Classification of Completely Randomized Design (CRD) with 4 treatments. Each treatment was repeated three times. Types of treatment in the form of using GCE and DCE, including: (1) control treatments (without GCE or DCE), (2) GCE, (3) DCE, and (4) a mixture of GCE and DCE. The observed variables were Coefficient of Dry Matter Digestibility (CDMD), Coefficient of Organic Matter Digestibility (COMD), and Coefficient of Soluble Protein Digestibility (CSPD). The results showed that the addition of GCE, DCE, and a mixture of GCE and DCE could not increase the CDMD, COMD, and CSPD of Turi Leaves compared to the control treatment. Keywords: Duodenal contents extract; Gizzard content extract; In vitro digestibility; Turi leaves.   Abstrak Penelitian bertujuan untuk mengkaji penggunaan cairan ekstrak isi gizzard (CEIZ) dan duodenum (CEID) ayam pada pengukuran kecernaan in vitro daun turi. Penelitian dirancang menggunakan Rancangan Acak Lengkap (RAL) pola searah dengan 4 perlakuan. Masing-masing perlakuan diulang tiga kali. Macam perlakuan berupa penggunaan penggunaan CEIZ dan CEID ayam, meliputi perlakuan kontrol (tanpa CEIZ maupun CEID), CEIZ, CEID, dan campuran CEIZ dan CEID. Variabel pengamatan berupa koefisien cerna bahan kering (KCBK), koefisien cerna bahan organic (KCBO), dan koefisien cerna protein terlarut (KCPT). Hasil penelitian menunjukkan bahwa perlakuan penambahan cairan ekstrak isi gizzard, duodenum dan campuran keduanya tidak dapat meningkatkan KCBK, KCBO, dan KCPT daun turi dibandingkan dengan perlakuan control. Kata kunci: Cairan ekstrak isi duodenum; Cairan ekstrak isi gizzard;  Daun turi; Kecernaan in vitro.

scholarly journals In Vitro Digestibility of Lamtoro Leaves (Leucaena Leucocephala) In Poultry With Gizzard Fluid And Duodenum

2021 ◽  
Vol 3 (2) ◽  
pp. 92
Author(s):  
Tri Ambarwati ◽  
Ali Mursyid Wahyu Mulyono ◽  
Engkus Ainul Yakin
Keyword(s):  
Organic Matter ◽  
Leucaena Leucocephala ◽  
Dry Matter ◽  
Protein Digestibility ◽  
In Vitro Digestibility ◽  
Duodenal Fluid ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Significant Difference

Protein source feed is the feed that has the biggest share in the nutritional needs of livestock. Lamtoro or Leucaena leucocephala is one of the leaf flours that can be used as an alternative feed ingredient for poultry in the tropics. With its abundant availability and does not compete for its needs with humans and has no selling value so that it can minimize consumption costs for poultry. This study aims to determine the effect of fluid from the digestive tract of chickens on the in vitro digestibility measurement of lamtoro leaves. The study used a completely randomized design (CRD) with a unidirectional pattern with 4 treatments and 3 replications. The treatments consisted of P0 = control, P1 = gizzard fluid, P2 = duodenal fluid and P3 = a mixture of gizzard and duodenal fluid. The results of this study show the average Dry Matter Digestibility Coefficient (%) for the treatment P0, P1, P2 and P3 respectively; 19.276, 29.089, 20.515 and 28.737. Organic Matter Digestibility Coefficient ; 27.702, 36.313, 24.795 and 35.649. Dissolved Protein Digestibility Coefficient ; 39.777, 53.288, 21.145 and 48.737. The results of the statistical test that the administration of lamtoro leaves showed a significant difference (P<0.05) on the digestibility coefficient of dry matter, organic matter and dissolved protein. The conclusion is that the use of gizzard liquid is more effective in increasing the in vitro digestibility of lamtoro leaves.

Laboratory analyses of the nutritional value of western Queensland browse feeds

1979 ◽  
Vol 19 (98) ◽  
pp. 344 ◽  
Author(s):  
WJF McDonald ◽  
JH Ternouth
Keyword(s):  
Organic Matter ◽  
Crude Protein ◽  
Dry Matter ◽  
In Vitro Digestibility ◽  
Crude Fibre ◽  
Dry Matter Digestibility ◽  
Nitrogen Free Extract ◽  
Organic Matter Digestibility

Seventy samples of browse feeds, collected during late autumn and spring of 1971 from 42 western Queensland shrubs and trees, were subjected to laboratory analyses of the proximate fractions and in vitro digestibility. Crude protein, crude fibre and nitrogen-free-extract fractions ranged between 65 and 242, 55 and 353 and 425 and 71 1 g kg-1 dry matter, repectively. The in vitro dry matter digestibility coefficients ranged between 0.22 and 0.86. Comparisons of 15 browse feeds collected in April-May and October indicated a higher crude protein content (P < 0.05) and in vitro digestibility (P .= 0.01) and lower crude fibre (P < 0.01) and ash (P < 0.01 ) content in spring samples for most species. Nine browse feeds of known in vivo dry matter and organic matter digestibility were subjected to in vitro digestibility analysis. Whilst the in vitro and in vivo dry matter digestibilities were similar (0.43 and 0.45, respectively) the mean in vitro organic matter digestibility was substantially lower than the corresponding in vivo digestibility (0.40 and 0.46, respectively).

A COMPARISON OF IN VITRO AND IN VIVO DRY MATTER DIGESTTBDLITY TECHNIQUES FOR THE EVALUATION OF FORAGE QUALITY

1987 ◽  
Vol 67 (2) ◽  
pp. 573-576 ◽  
Author(s):  
E. V. VALDES ◽  
G. E. JONES
Keyword(s):  
Key Words ◽  
Dry Matter ◽  
Forage Quality ◽  
In Vitro Digestibility ◽  
Two Stage ◽  
Dry Matter Digestibility ◽  
Whole Plant ◽  
Standard Deviations

A two-stage cellulase-pepsin technique (CS) was compared with the two-stage rumen inoculum-pepsin (IVDMD) method to predict in vivo dry matter digestibility (in vivo DMD) in grasses (n = 30) and legumes (n = 25). Comparisons of CS with IVDMD in whole-plant corn (n = 186) were also conducted. There were significant correlations (P < 0.01) between CS and in vivo DMD and between CS and IVDMD in all forages. The coefficients of determination (r2) and residual standard deviations for the prediction of IVDMD by CS varied between 0.76 and 0.90 and 2.5 and 3.4, respectively. Key words: Digestibility (in vitro), digestibility (in vivo), fungal cellulases, forage

scholarly journals The Effect of Zinc-Proteinate Supplementation on the In Vitro Digestibility and Ruminal Fermentation in Goat

2021 ◽  
pp. 180-186
Author(s):  
Gilbert Nathaniel ◽  
Tiara Annisa ◽  
Anis Muktiani ◽  
Dian Wahyu Harjanti ◽  
Widiyanto Widiyanto
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Experimental Treatment ◽  
Dairy Goat ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Organic Matter Digestibility

This study aimed primarily to investigate the effect of Zn-Proteinate (Zn-Prot) supplementation on in vitro rumen digestibility and rumen fermentation. This research used a completely randomized design with four treatments and four replicates. The experimental treatment was the supplementation of various levels of Zn-Prot (0; 12.5 ppm; 25 ppm, and 37.5 ppm) on a dry matter basis. Parameters determined were in vitro dry matter digestibility (IVDMD), in vitro organic matter digestibility (IVOMD), partial volatile fatty acid (VFA) (acetate, propionate, and butyrate), A/P ratio, CH4, and the efficiency of the conversion hexose to VFA. Data were analyzed using ANOVA. The results showed that goats fed with a diet supplemented with 25 ppm Zn-Prot had the highest IVDMD and IVOMD values. There was no significant effect on the VFA and CH4 concentrations, A/P ratio, and the efficiency of the hexose-VFA conversion within treatment groups. In conclusion, supplementing 25 ppm of Zn-Prot into the diet of dairy goat increase the dry matter and organic matter digestibility. 

scholarly journals Evaluation of in vitro digestibility and pH of Acacia seed pods (Acacia sp.)

2021 ◽  
Vol 902 (1) ◽  
pp. 012003
Author(s):  
R F Hadi ◽  
E Handayanta ◽  
I Ngadyastuti
Keyword(s):  
Ph Value ◽  
Rumen Fluid ◽  
Acacia Mangium ◽  
Acacia Auriculiformis ◽  
In Vitro Digestibility ◽  
Acacia Species ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Two Stages

Abstract This study aimed to determine the rumen fluid’s in-vitro dry matter digestibility (IVDMD), in-vitro organic matter digestibility (IVOMD), and pH of different species Acacia seed pods as a single feed ingredient. The Acacia species in this study were Acacia mangium, Acacia auriculiformis, and Acacia crassicarpa. The method used was in vitro two-stages with the first 48 hours of incubation (the rumen) and the second 48 hours of incubation (post-rumen), and analyses of pH. The research design used was a completely randomized design (CRD) with 3 treatments and 5 replications based on the Acacia species. The data were analysed with oneway analysis of variance (ANOVA) followed by Duncan’s multiple range test (DMRT). The results showed that IVDMD stage one was 34.59-37.59%; IVOMD stage one was 38.66-43.06%. Stage two of IVDMD was 58.02-59.23%; stage two of IVOMD was 51.67-55.01% and rumen fluid pH were 6.58-7.02. Different Acacia seed pods had significant differences in IVDMD and IVOMD stage one in the rumen and stage two in the post-rumen (P<0.05), but not in the pH value (P>0.05). We concluded that different acacia seed pods had different digestibility values in the rumen and post-rumen. However, it does not affect the rumen acidity.

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