scholarly journals The Effect of Zinc-Proteinate Supplementation on the In Vitro Digestibility and Ruminal Fermentation in Goat

2021 ◽  
pp. 180-186
Author(s):  
Gilbert Nathaniel ◽  
Tiara Annisa ◽  
Anis Muktiani ◽  
Dian Wahyu Harjanti ◽  
Widiyanto Widiyanto

This study aimed primarily to investigate the effect of Zn-Proteinate (Zn-Prot) supplementation on in vitro rumen digestibility and rumen fermentation. This research used a completely randomized design with four treatments and four replicates. The experimental treatment was the supplementation of various levels of Zn-Prot (0; 12.5 ppm; 25 ppm, and 37.5 ppm) on a dry matter basis. Parameters determined were in vitro dry matter digestibility (IVDMD), in vitro organic matter digestibility (IVOMD), partial volatile fatty acid (VFA) (acetate, propionate, and butyrate), A/P ratio, CH4, and the efficiency of the conversion hexose to VFA. Data were analyzed using ANOVA. The results showed that goats fed with a diet supplemented with 25 ppm Zn-Prot had the highest IVDMD and IVOMD values. There was no significant effect on the VFA and CH4 concentrations, A/P ratio, and the efficiency of the hexose-VFA conversion within treatment groups. In conclusion, supplementing 25 ppm of Zn-Prot into the diet of dairy goat increase the dry matter and organic matter digestibility. 

2016 ◽  
Vol 1 (1) ◽  
pp. 808-815
Author(s):  
Sugrahadi Ahmad Aprianto ◽  
Yunasri Usman ◽  
Asril Asril

Abstrak: Penelitian ini telah dilaksanakan di Laboratorium Ilmu Nutrisi dan Makanan Ternak Program Studi Peternakan Fakultas Pertanian Universitas Syiah Kuala dan dilanjutkan dengan pengiriman sampel penelitian ke Laboratorium Ternak Perah Institut Pertanian Bogor (IPB) untuk pengujian kecernaan secara in vitro. Tujuan dari penelitian ini adalah menguji kecernaan secara in vitro complete feed berbahan dasar ampas sagu dengan teknik fermentasi yang berbeda.Pembuatan complete feed disusun sesuai dengan kebutuhan ternak domba. Penelitian ini menggunakan Rancangan Acak lengkap (RAL) dengan 3 perlakuan dan masing-masing perlakuan terdiri atas 5 ulangan. Perlakuan 1 (kontrol) adalah complete feed tanpa penambahan Saus Burger Pakan (SBP) dan tanpa fermentasi, perlakuan 2 adalah ampas sagu difermentasi dengan SBP selama 14 hari kemudian dicampur menjadi complete feed dan difermentasi hingga 21 hari, sedangkan perlakuan 3 adalah ampas sagu + complete feed kemudian difermentasi dengan SBP selama 21 hari. Parameter yang diamati dalam penelitian ini adalah pH, Bahan Kering (BK), Bahan Organik (BO), Koefisien Cerna Bahan Kering (KCBK), dan Koefisien Cerna Bahan Organik (KCBO). Hasil pengamatan menyimpulkan bahwa, teknik fermentasi yang berbeda berpengaruh sangat nyata (P0,01) terhadap semua parameter yang diamati, yaitu pH, BK, BO, KCBK dan KCBO. Dari hasil penelitian dapat disimpulkan bahwa nilai kecernaan complete feed berbahan dasar ampas sagu dipengaruhi oleh perbedaan teknik fermentasi. Digestibility Evaluation In Vitro Complete Feed Fermentation Sago Residues Based with Different Fermentation Techniques Abstract: This research was conducted at the Laboratory of Nutrition and Feed Studies, Program study Animal Husbandry, Faculty of Agriculture, University of Syiah Kuala and continued with the delivery of sample to the Laboratory of Dairy Cattle Institute Pertanian Bogor (IPB) for testing of in vitro digestibility. The purpose of this study was to test in vitro digestibility of complete feed made from  the residues of sago with different fermentation techniques. Complete feed was arranged based on the needs of sheep. This study used a complete randomized design (CRD) with 3 treatments and each treatment consisted of 5 replicates. Treatment 1 (control) was a complete feed without the addition of Saus Burger Pakan (SBP) and without fermentation, treatment 2 was sago residu  fermented with SBP for 14 days and then mixed into a complete feed and fermented up to 21 days, whereas treatment 3 was the sago residue + complete feed then fermented with SBP for 21 days. The parameters observed in this study were pH, Dry Matter (BK), Organic Matter (BO), Dry Matter digestibility coefficients (KCBK), and Organic Matter Digestibility Coefficient (KCBO). The result influence showed that, different fermentation technique was significantly (P 0.01) all parameters, namely pH, BK, BO, KCBK and KCBO. It can be concluded that the digestibility of complete feed made from sago residue influenced by differences in fermentation techniques


Author(s):  
Chabib B.H ◽  
Sri Rahayu ◽  
Muhamad Bata

This experiment investigated various substrate growth of bamboo stems microbes (BSM) in rice straw fermentation and its effect on dry matter digestibility (DMD), organic matter digestibility (OMD), NH3-N and VFA concentration. Materials were rumen fluid of beef cattle from the slaughterhouse. Basal diets consisted of rice straw and concentrate with ratio of 40 : 60 (% dry matter) were administered to five treatments namely P0 = untreated rice straw (control), P1 = Ammoniated rice straw,  fermented rice straw using microbial bamboo stems grown on ammoniated rice straw (P2), rice bran (P3) and cassava waste (P4). The experiment used in vitro method was designed with Completely Randomized Design and the data were analyzed variance. The results showed that the treatment had no significant effect on dry matter digestibility (DMD), organic matter digestibility (OMD), NH3-N and VFA concentration (P>0,05). The fermentation process of microbial bamboo stems in rice straw with a level of 10% with different substrate did not able to  increase the digestibility and products fermentation, but there tends that the rice bran and substrates can increase DMD and N-NH3 concentration


2021 ◽  
Vol 3 (2) ◽  
pp. 92
Author(s):  
Tri Ambarwati ◽  
Ali Mursyid Wahyu Mulyono ◽  
Engkus Ainul Yakin

Protein source feed is the feed that has the biggest share in the nutritional needs of livestock. Lamtoro or Leucaena leucocephala is one of the leaf flours that can be used as an alternative feed ingredient for poultry in the tropics. With its abundant availability and does not compete for its needs with humans and has no selling value so that it can minimize consumption costs for poultry. This study aims to determine the effect of fluid from the digestive tract of chickens on the in vitro digestibility measurement of lamtoro leaves. The study used a completely randomized design (CRD) with a unidirectional pattern with 4 treatments and 3 replications. The treatments consisted of P0 = control, P1 = gizzard fluid, P2 = duodenal fluid and P3 = a mixture of gizzard and duodenal fluid. The results of this study show the average Dry Matter Digestibility Coefficient (%) for the treatment P0, P1, P2 and P3 respectively; 19.276, 29.089, 20.515 and 28.737. Organic Matter Digestibility Coefficient ; 27.702, 36.313, 24.795 and 35.649. Dissolved Protein Digestibility Coefficient ; 39.777, 53.288, 21.145 and 48.737. The results of the statistical test that the administration of lamtoro leaves showed a significant difference (P<0.05) on the digestibility coefficient of dry matter, organic matter and dissolved protein. The conclusion is that the use of gizzard liquid is more effective in increasing the in vitro digestibility of lamtoro leaves.


2019 ◽  
Vol 19 (2) ◽  
pp. 85-90
Author(s):  
Asep Saripudin ◽  
Shena Nurpauza ◽  
Budi Ayuningsih ◽  
Iman Hernaman ◽  
Ana Rochana Tarmidi

ABSTRAK. Penelitian bertujuan untuk mengetahui fermentabilitas dan kecernaan ransum domba yang mengandung limbah roti. Penelitian menggunakan rancangan acak lengkap dengan lima macam ransum perlakuan yang terdiri atas 40% rumput lapangan dan 60% konsentrat yang masing-masing mengandung limbah roti sebanyak 0 (kontrol), 10, 20, 30, dan 40%. Masing-masing perlakuan diulang sebanyak 4 kali dan data yang terkumpul dianalisis dengan analisis sidik ragam dan dilanjutkan dengan uji Duncan. Ransum perlakuan dievaluasi secara in vitro. Peubah yang diukur adalah asam lemak terbang (ALT), N-NH3, kecernaan bahan kering, dan kecernaan bahan organik. Hasil penelitian menunjukkan bahwa penggunaan limbah roti dalam ransum domba sampai 40% mampu meningkatkan konsentrasi ALT (102,63 - 143,88 mM), kecernaan bahan kering (64,66 - 78,61%) dan kecernaan bahan organik (53,41 - 65,82%). Sementara itu, terjadi kenaikan konsentrasi N-NH3 (3,87 - 4,90 mM) sampai penggunaan limbah roti 30%, namun penggunaan 40% limbah roti sama dengan ransum kontrol. Konsentrasi ALT memiliki hubungan erat dengan kecernaan bahan kering dan bahan organik dengan nilai r = 0,67 dan 0,65, pada persamaan regresi Y = 0,1591X + 50,79 dan Y = 0,1528X + 39,619. Kesimpulan, Penggunaan limbah roti di dalam ransum sebanyak 40% tidak mengganggu fermentabilitas dan kecernaan ransum secara in vitro.  (In vitro fermentability and digestibility of sheep rations containing bread waste)ABSTRACT. The study aimed to determine the fermentability and digestibility of sheep rations containing bread waste. The study used a completely randomized design with five types of treatment rations consisting of 40% native grass and 60% concentrate and each treatment containing bread waste as much as 0 (control), 10, 20, 30, and 40%. Each treatment was repeated 4 times and the data collected was analyzed by analysis of variance and continued by Duncan test. The treatment ration was evaluated by in vitro. The variables measured were volatile fatty acids (VFA), N-NH3, dry matter digestibility, and organic matter digestibility. The results showed that the use of bread waste in sheep rations up to 40% was able to increase the concentration of VFA (102.63 to 143.88 mM), dry matter (64.66 to 78.61%) and organic matter digestibility (53.41 to 65.82%). Meanwhile, there was an increase in the concentration of N-NH3 (3.87 to 4.90 mM) until 30%, but the use of 40% bread waste was the same as the control ration. The VFA concentration has a close relationship with the dry matter and organic matter digestibility with values of r = 0.67 and 0.65, in the regression equation Y = 0.1591X + 50.79 and Y = 0.1528X + 39.619. The conclusion is using of bread waste as much as 40% did not disturb the fermentability and digestibility of the ration in vitro.


Pastura ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 29
Author(s):  
A.A.A.S. Trisnadewi ◽  
I G.L. Oka Cakra ◽  
T.G.B. Yadnya

The study aimed to determine invitro digestibility, volattyle fatty acid (VFA) and ammonia (NH3) of corn straw silage with different storage periode. The experiments use a completely randomized design (CRD) with four treatments and each treatment was repeated five times, so there are 20 experimental units. The fourth treatments are W1 = storage time of 14 days, W2 = storage time of 21 days, W3 = storage time of 28 days, and W4 = storage time of 35 days. The parameters observed in this study in-vitro digestibility including dry matter and organic matter digestibility, VFA, and NH3. Results of the experiment showed that dry matter digestibility showed significant differences, otherwise organic matter digestibility, VFA, and NH3 were not significantly different, and there was a tendency to decrease the value of dry matter and organic matter digestibility, VFA, and NH3 with longer duration of storage of corn straw silage. It can be concluded that dry matter, organic matter digestibility VFA, and NH3, are not significant different. Keywords: corn straw, silage, storage periode, invitro digestibility, VFA, NH3


2018 ◽  
Vol 5 (2) ◽  
Author(s):  
A. Fariani., dkk

This research was done to improve coffee pulp digestibility with alkali treatment and adding organic acid by in vitro. This research was done in two stage, fi rst stage was soaking in alkali fl uid and second stage was in vitro digestibility analysis, in Animal Feed and Nutrition Laboratory, Faculty of Agriculture, Sriwijaya University. Completely Randomized Design (CRD) used in 4 reatments and feed treatment was replicated 3 times. The treatments were: P0 (control), P1 (2 day soaking), P2 (3 day soaking) and P3 (4 day soaking). Observe parameters were tannin concentration, dry matter digestibility, organic matter digestibility, concentration of N-NH3 and (pH). The result showed that the treatment had nosignifi cant diference (P0.01) on tannin concentration. However, it was signifi cantly difference on dry matter digestibility, organic matter digestibility, concentration of N-NH3, and (pH).Key Words: Coffee pulp, alkali treatment, digestibility, in vitro techniques


1979 ◽  
Vol 19 (98) ◽  
pp. 344 ◽  
Author(s):  
WJF McDonald ◽  
JH Ternouth

Seventy samples of browse feeds, collected during late autumn and spring of 1971 from 42 western Queensland shrubs and trees, were subjected to laboratory analyses of the proximate fractions and in vitro digestibility. Crude protein, crude fibre and nitrogen-free-extract fractions ranged between 65 and 242, 55 and 353 and 425 and 71 1 g kg-1 dry matter, repectively. The in vitro dry matter digestibility coefficients ranged between 0.22 and 0.86. Comparisons of 15 browse feeds collected in April-May and October indicated a higher crude protein content (P < 0.05) and in vitro digestibility (P .= 0.01) and lower crude fibre (P < 0.01) and ash (P < 0.01 ) content in spring samples for most species. Nine browse feeds of known in vivo dry matter and organic matter digestibility were subjected to in vitro digestibility analysis. Whilst the in vitro and in vivo dry matter digestibilities were similar (0.43 and 0.45, respectively) the mean in vitro organic matter digestibility was substantially lower than the corresponding in vivo digestibility (0.40 and 0.46, respectively).


2021 ◽  
Vol 16 (4) ◽  
pp. 362-367
Author(s):  
A. A. Widu ◽  
F. U. Datta ◽  
M. M. Kleden

This study was conducted to determine the effect of different levels of binahong flour used  in concentrate feed on dry matter digestibility (DMD), organic matter digestibility (OMD), VFA and NH3 concentrations in vitro. The design used was a completely randomized design with 4 treatments and 4 replications. The treatments studied were: R0; concentrate feed without binahong flour, R1; concentrate feed contains 10% binahong flour, R2; concentrate feed containing 20% binahong flour, R3; concentrate feed contains 30% binahong flour. Based on the results of the study, the average DMD (%) R0 73.56±0.75, R1 74.50 ±0.61, R2 74.98±0.70, R3 76.65±0.90, OMD (%) R0 69.50±1.10, R1 69.82±1.00, R2 70.07±0.88, R3 72.61±0.99, VFA concentration (mM) R0 108.53±8.52, R1 110.75±6.51, R2 111.40±2.35, R3 112.77 ±14.76, NH3 concentration (mM) R0 9.74±0.74, R1 10.61±0.44, R2 11.55±0.41, R3 11.55±0.33. The results of statistical analysis confirmed that the treatment had a very significant effect on the increase in DMD, OMD, NH3 (P<0,01), but not significant on the total VFA concentration. The conclusion of this study is that the use of binahong flour up to a level of 30% in concentrate feed has an effect on increasing in DMD, OMD, VFA, and NH3 concentrations in vitro.


2020 ◽  
Vol 44 (1) ◽  
Author(s):  
Monica Canadianti ◽  
Lies Mira Yusiati ◽  
Chusnul Hanim ◽  
Budi Prasetyo Widyobroto ◽  
Andriyani Astuti

This experiment was aimed to study the effect of nutmeg leaf tannin addition on in vitro nutrient digestibility. Treatments in this experiment consisted of: P0 (control without tannin), P1 (feed + 2% tannin) and P2 (feed + 4% tannin). Feed for fermentation substrate consisted of Pennisetum purpureum and soybean meal with ratio 60:40. Fermentation was carried out using Tilley and Terry two stages in vitro technique for 48 hours. Variables measured were the digestibility of dry matter, organic matter, and crude protein in the rumen as well as the total digestive tract digestibility based on in vitro technique. The data obtained were analyzed by One Way ANOVA, and followed by the Duncan Multiple Range Test (DMRT). The results showed that rumen dry matter digestibility was lower (P<0.05) in P1 and P2 (59.03±3.24 and 57.19±1.32) compared to P0 (70.77±1.05), but did not show a significant difference (P>0.05) in the total dry matter digestibility of P0, P1, and P2 (74.88±5.28, 67.70±3.21, and 64.83±4.96). Organic matter digestibility in the rumen was also lower (P<0.05) in P1 and P2 (55.55±6.29 and 55.76±6.88) compared to P0 (75.39±0.91), but did not show significant difference (P>0.05) in total organic matter digestibility from P0, P1, and P2 (64.69±6.44, 64.33±6.34, and 61.20±5.11). The digestibility of crude protein in the rumen at P1 and P2 (45.48±5.12 and 38.47±3.44) was also significantly lower (P<0.05) compared to P0 (60.93±9.72), whereas total digestibility did not show any significant difference (P>0.05). Addition of tannin leaf nutmeg 2% optimally reduced rumen dry matter and crude protein digestibility without causing excessive negative impact on results of in vitro digestibility, so it can be used as a protective agent protein feed.


2015 ◽  
Vol 2 (1) ◽  
pp. 54
Author(s):  
Erwin Yulianto ◽  
Andi Murlina Tasse ◽  
Rahman Rahman

This study aims to know the effect of addition of Drymoglosum pilloseloides flour on in vitro digestibility of consentrates made from fermentation feed. The treatments consist of concentrates ware added Drymoglosum pilloseloides flour 0% (R0), 0,05% (R1), 0,1% (R2), and 0,15% (R3). Experimental design was used completely randomized design (CRD) with 4 treatments and 3 replications. This study was conducted during four weeks in Animal Science Department, of Animal Science Faculty, of Haluoleo University. The average of water content of each treatment was 15,50% ± 2,56% (R0), 16,17% ± 0,32% (R1), 17,80% ± 1,57% (R2) and 16,83% ± 1,58% (R3), ash content was 25,90% ± 0,72% (R0), 21,40% ± 0,52% (R1), 20,07% ± 0,25% (R2) and 18,50% ± 0,53% (R3), dry matter digestibility was 81,33% ± 1,00% (R0), 81,73% ± 0,49% (R1), 82,27% ± 0,91% (R2) and 81,40% ± 0,10% (R3), organic matter digestibility was 81,70% ± 1,31% (R0), 83,90% ± 1,59% (R1), 83,17% ± 0,90% (R2) and 82,50% ± 0,46% (R3). The result of analysis of variance not showed that addition of Drymoglosum pilloseloides flour 0%, 0,05%, 0,10% and 0,15% significant effect (P>0,05) on water content, dry matter digestibility and organic matter digestibility. While concentrates with addition of Drymoglosum pilloseloides flour 0%, 0,05%, 0,10% and 0,15% significant effect (P<0,05) on ash content. Dry matter and organic matter digestibility were higher than normal range that usually be given to animal. Therefore concentrates with addition of Drymoglosum pilloseloides flour no decreased dry matter and dry organic digestibilities.Key Words: Drymoglosum pilloseloides, fermented feed, dry matter digestibilityThis study aims to know the effect of addition of Drymoglosum pilloseloides flour on in vitro digestibility of consentrates made from fermentation feed. The treatments consist of concentrates ware added Drymoglosum pilloseloides flour 0% (R0), 0,05% (R1), 0,1% (R2), and 0,15% (R3). Experimental design was used completely randomized design (CRD) with 4 treatments and 3 replications. This study was conducted during four weeks in Animal Science Department, of Animal Science Faculty, of Haluoleo University. The average of water content of each treatment was 15,50% ± 2,56% (R0), 16,17% ± 0,32% (R1), 17,80% ± 1,57% (R2) and 16,83% ± 1,58% (R3), ash content was 25,90% ± 0,72% (R0), 21,40% ± 0,52% (R1), 20,07% ± 0,25% (R2) and 18,50% ± 0,53% (R3), dry matter digestibility was 81,33% ± 1,00% (R0), 81,73% ± 0,49% (R1), 82,27% ± 0,91% (R2) and 81,40% ± 0,10% (R3), organic matter digestibility was 81,70% ± 1,31% (R0), 83,90% ± 1,59% (R1), 83,17% ± 0,90% (R2) and 82,50% ± 0,46% (R3). The result of analysis of variance not showed that addition of Drymoglosum pilloseloides flour 0%, 0,05%, 0,10% and 0,15% significant effect (P>0,05) on water content, dry matter digestibility and organic matter digestibility. While concentrates with addition of Drymoglosum pilloseloides flour 0%, 0,05%, 0,10% and 0,15% significant effect (P<0,05) on ash content. Dry matter and organic matter digestibility were higher than normal range that usually be given to animal. Therefore concentrates with addition of Drymoglosum pilloseloides flour no decreased dry matter and dry organic digestibilities.Key Words: Drymoglosum pilloseloides, fermented feed, dry matter digestibility


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