scholarly journals In Vitro Digestibility of Lamtoro Leaves (Leucaena Leucocephala) In Poultry With Gizzard Fluid And Duodenum

2021 ◽  
Vol 3 (2) ◽  
pp. 92
Author(s):  
Tri Ambarwati ◽  
Ali Mursyid Wahyu Mulyono ◽  
Engkus Ainul Yakin

Protein source feed is the feed that has the biggest share in the nutritional needs of livestock. Lamtoro or Leucaena leucocephala is one of the leaf flours that can be used as an alternative feed ingredient for poultry in the tropics. With its abundant availability and does not compete for its needs with humans and has no selling value so that it can minimize consumption costs for poultry. This study aims to determine the effect of fluid from the digestive tract of chickens on the in vitro digestibility measurement of lamtoro leaves. The study used a completely randomized design (CRD) with a unidirectional pattern with 4 treatments and 3 replications. The treatments consisted of P0 = control, P1 = gizzard fluid, P2 = duodenal fluid and P3 = a mixture of gizzard and duodenal fluid. The results of this study show the average Dry Matter Digestibility Coefficient (%) for the treatment P0, P1, P2 and P3 respectively; 19.276, 29.089, 20.515 and 28.737. Organic Matter Digestibility Coefficient ; 27.702, 36.313, 24.795 and 35.649. Dissolved Protein Digestibility Coefficient ; 39.777, 53.288, 21.145 and 48.737. The results of the statistical test that the administration of lamtoro leaves showed a significant difference (P<0.05) on the digestibility coefficient of dry matter, organic matter and dissolved protein. The conclusion is that the use of gizzard liquid is more effective in increasing the in vitro digestibility of lamtoro leaves.

2015 ◽  
Vol 2 (3) ◽  
pp. 17
Author(s):  
Indrayani Indrayani ◽  
Harapin Hafid ◽  
Dian Agustina

ABSTRACTThis study aims to determine the digestibility of dry matter and organic matter level waste silage mixed vegetables and Gliricidia leaves were tested in vitro. This study used a completely randomized design with 3 treatments and 3 replications. Each of these treatments is R0 (Gliricidia leaves 100%), R1 (Gliricidia leaves 70% + 30% silage vegetable waste), and R2 (Gliricidia leaves 40% + 60% silage vegetable waste). Data were analyzed using analysis of variance (ANOVA) and further testing using the test Honestly Significant Difference (HSD). The results of ANOVA showed that the mixture of vegetable waste silage was highly significant (p <0.05) on dry matter digestibility and significantly (p <0.05) on the digestibility of organic matter. It can be concluded that the mixed of vegetable waste silage and Gliricidia leaves can improved digestibility of dry matter and organic matter, treatment of 40% and 60% Gliricidia leaves plus waste vegetable produce silage dry matter digestibility and percentage of organic matter is best (72,24% and 68,19%).Keyword: Silage vegetable waste, gliricidia leaves, dry and organic matter digestibilityABSTRAKPenelitian ini bertujuan untuk mengetahui kecenaan bahan kering dan bahan organik tingkat campuran silase sampah sayur dan daun gamal yang diuji secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 3 perlakuan dan 3 ulangan. Masing-masing perlakuan tersebut ialah R0 (daun gamal 100%), R1 (daun gamal 70% + silase sampah sayur 30%), dan R2 (daun gamal 40% + silase sampah sayur 60%). Data yang diperoleh dianalisis dengan menggunakan analisis sidik ragam (ANOVA) dan uji lanjut menggunakan uji Beda Nyata Jujur (BNJ). Hasil analisis sidik ragam menunjukkan bahwa campuran silase sampah sayur berpengaruh sangat nyata (p<0,05) terhadap kecernaan bahan kering dan berpengaruh nyata (p<0,05) terhadap kecernaan bahan organik. Dapat disimpulkan bahwa perlakuan campuran silase sampah sayur dan daun gamal dapat meningkatkan kecernaan bahan kering dan bahan organik, perlakuan 40% daun gamal dan 60% silase sampah sayur menghasilkan persentase kecernaan bahan kering dan bahan organik yang terbaik yaitu (72,24% dan 68,19%).Kata kunci : Silase sampah sayur, daun gamal, kecernaan bahan kering, dan bahan organik.


2016 ◽  
Vol 1 (1) ◽  
pp. 808-815
Author(s):  
Sugrahadi Ahmad Aprianto ◽  
Yunasri Usman ◽  
Asril Asril

Abstrak: Penelitian ini telah dilaksanakan di Laboratorium Ilmu Nutrisi dan Makanan Ternak Program Studi Peternakan Fakultas Pertanian Universitas Syiah Kuala dan dilanjutkan dengan pengiriman sampel penelitian ke Laboratorium Ternak Perah Institut Pertanian Bogor (IPB) untuk pengujian kecernaan secara in vitro. Tujuan dari penelitian ini adalah menguji kecernaan secara in vitro complete feed berbahan dasar ampas sagu dengan teknik fermentasi yang berbeda.Pembuatan complete feed disusun sesuai dengan kebutuhan ternak domba. Penelitian ini menggunakan Rancangan Acak lengkap (RAL) dengan 3 perlakuan dan masing-masing perlakuan terdiri atas 5 ulangan. Perlakuan 1 (kontrol) adalah complete feed tanpa penambahan Saus Burger Pakan (SBP) dan tanpa fermentasi, perlakuan 2 adalah ampas sagu difermentasi dengan SBP selama 14 hari kemudian dicampur menjadi complete feed dan difermentasi hingga 21 hari, sedangkan perlakuan 3 adalah ampas sagu + complete feed kemudian difermentasi dengan SBP selama 21 hari. Parameter yang diamati dalam penelitian ini adalah pH, Bahan Kering (BK), Bahan Organik (BO), Koefisien Cerna Bahan Kering (KCBK), dan Koefisien Cerna Bahan Organik (KCBO). Hasil pengamatan menyimpulkan bahwa, teknik fermentasi yang berbeda berpengaruh sangat nyata (P0,01) terhadap semua parameter yang diamati, yaitu pH, BK, BO, KCBK dan KCBO. Dari hasil penelitian dapat disimpulkan bahwa nilai kecernaan complete feed berbahan dasar ampas sagu dipengaruhi oleh perbedaan teknik fermentasi. Digestibility Evaluation In Vitro Complete Feed Fermentation Sago Residues Based with Different Fermentation Techniques Abstract: This research was conducted at the Laboratory of Nutrition and Feed Studies, Program study Animal Husbandry, Faculty of Agriculture, University of Syiah Kuala and continued with the delivery of sample to the Laboratory of Dairy Cattle Institute Pertanian Bogor (IPB) for testing of in vitro digestibility. The purpose of this study was to test in vitro digestibility of complete feed made from  the residues of sago with different fermentation techniques. Complete feed was arranged based on the needs of sheep. This study used a complete randomized design (CRD) with 3 treatments and each treatment consisted of 5 replicates. Treatment 1 (control) was a complete feed without the addition of Saus Burger Pakan (SBP) and without fermentation, treatment 2 was sago residu  fermented with SBP for 14 days and then mixed into a complete feed and fermented up to 21 days, whereas treatment 3 was the sago residue + complete feed then fermented with SBP for 21 days. The parameters observed in this study were pH, Dry Matter (BK), Organic Matter (BO), Dry Matter digestibility coefficients (KCBK), and Organic Matter Digestibility Coefficient (KCBO). The result influence showed that, different fermentation technique was significantly (P 0.01) all parameters, namely pH, BK, BO, KCBK and KCBO. It can be concluded that the digestibility of complete feed made from sago residue influenced by differences in fermentation techniques


Author(s):  
Ali Mursyid Wahyu Mulyono ◽  
Sri Sukar Yani ◽  
Jizan Fahmia Al Awanis

Abstract The aim of this study was to examine the use of Gizzard Contents Extracts (GCE) and Duodenum Contents Extracts (DCE) of Chicken on In Vitro digestibility measurements of Turi Leaves (Sesbania grandiflora). The study was designed using a One-way Classification of Completely Randomized Design (CRD) with 4 treatments. Each treatment was repeated three times. Types of treatment in the form of using GCE and DCE, including: (1) control treatments (without GCE or DCE), (2) GCE, (3) DCE, and (4) a mixture of GCE and DCE. The observed variables were Coefficient of Dry Matter Digestibility (CDMD), Coefficient of Organic Matter Digestibility (COMD), and Coefficient of Soluble Protein Digestibility (CSPD). The results showed that the addition of GCE, DCE, and a mixture of GCE and DCE could not increase the CDMD, COMD, and CSPD of Turi Leaves compared to the control treatment. Keywords: Duodenal contents extract; Gizzard content extract; In vitro digestibility; Turi leaves.   Abstrak Penelitian bertujuan untuk mengkaji penggunaan cairan ekstrak isi gizzard (CEIZ) dan duodenum (CEID) ayam pada pengukuran kecernaan in vitro daun turi. Penelitian dirancang menggunakan Rancangan Acak Lengkap (RAL) pola searah dengan 4 perlakuan. Masing-masing perlakuan diulang tiga kali. Macam perlakuan berupa penggunaan penggunaan CEIZ dan CEID ayam, meliputi perlakuan kontrol (tanpa CEIZ maupun CEID), CEIZ, CEID, dan campuran CEIZ dan CEID. Variabel pengamatan berupa koefisien cerna bahan kering (KCBK), koefisien cerna bahan organic (KCBO), dan koefisien cerna protein terlarut (KCPT). Hasil penelitian menunjukkan bahwa perlakuan penambahan cairan ekstrak isi gizzard, duodenum dan campuran keduanya tidak dapat meningkatkan KCBK, KCBO, dan KCPT daun turi dibandingkan dengan perlakuan control. Kata kunci: Cairan ekstrak isi duodenum; Cairan ekstrak isi gizzard;  Daun turi; Kecernaan in vitro.


2020 ◽  
Vol 44 (1) ◽  
Author(s):  
Monica Canadianti ◽  
Lies Mira Yusiati ◽  
Chusnul Hanim ◽  
Budi Prasetyo Widyobroto ◽  
Andriyani Astuti

This experiment was aimed to study the effect of nutmeg leaf tannin addition on in vitro nutrient digestibility. Treatments in this experiment consisted of: P0 (control without tannin), P1 (feed + 2% tannin) and P2 (feed + 4% tannin). Feed for fermentation substrate consisted of Pennisetum purpureum and soybean meal with ratio 60:40. Fermentation was carried out using Tilley and Terry two stages in vitro technique for 48 hours. Variables measured were the digestibility of dry matter, organic matter, and crude protein in the rumen as well as the total digestive tract digestibility based on in vitro technique. The data obtained were analyzed by One Way ANOVA, and followed by the Duncan Multiple Range Test (DMRT). The results showed that rumen dry matter digestibility was lower (P<0.05) in P1 and P2 (59.03±3.24 and 57.19±1.32) compared to P0 (70.77±1.05), but did not show a significant difference (P>0.05) in the total dry matter digestibility of P0, P1, and P2 (74.88±5.28, 67.70±3.21, and 64.83±4.96). Organic matter digestibility in the rumen was also lower (P<0.05) in P1 and P2 (55.55±6.29 and 55.76±6.88) compared to P0 (75.39±0.91), but did not show significant difference (P>0.05) in total organic matter digestibility from P0, P1, and P2 (64.69±6.44, 64.33±6.34, and 61.20±5.11). The digestibility of crude protein in the rumen at P1 and P2 (45.48±5.12 and 38.47±3.44) was also significantly lower (P<0.05) compared to P0 (60.93±9.72), whereas total digestibility did not show any significant difference (P>0.05). Addition of tannin leaf nutmeg 2% optimally reduced rumen dry matter and crude protein digestibility without causing excessive negative impact on results of in vitro digestibility, so it can be used as a protective agent protein feed.


2021 ◽  
pp. 180-186
Author(s):  
Gilbert Nathaniel ◽  
Tiara Annisa ◽  
Anis Muktiani ◽  
Dian Wahyu Harjanti ◽  
Widiyanto Widiyanto

This study aimed primarily to investigate the effect of Zn-Proteinate (Zn-Prot) supplementation on in vitro rumen digestibility and rumen fermentation. This research used a completely randomized design with four treatments and four replicates. The experimental treatment was the supplementation of various levels of Zn-Prot (0; 12.5 ppm; 25 ppm, and 37.5 ppm) on a dry matter basis. Parameters determined were in vitro dry matter digestibility (IVDMD), in vitro organic matter digestibility (IVOMD), partial volatile fatty acid (VFA) (acetate, propionate, and butyrate), A/P ratio, CH4, and the efficiency of the conversion hexose to VFA. Data were analyzed using ANOVA. The results showed that goats fed with a diet supplemented with 25 ppm Zn-Prot had the highest IVDMD and IVOMD values. There was no significant effect on the VFA and CH4 concentrations, A/P ratio, and the efficiency of the hexose-VFA conversion within treatment groups. In conclusion, supplementing 25 ppm of Zn-Prot into the diet of dairy goat increase the dry matter and organic matter digestibility. 


2009 ◽  
Vol 49 (7) ◽  
pp. 563 ◽  
Author(s):  
David B. Coates ◽  
Robert J. Mayer

In a study that included C4 tropical grasses, C3 temperate grasses and C3 pasture legumes, in vitro dry matter digestibility of extrusa, measured as in vitro dry matter loss (IVDML) during incubation, compared with that of the forage consumed, was greater for grass extrusa but not for legume extrusa. The increase in digestibility was not caused by mastication or by the freezing of extrusa samples during storage but by the action of saliva. Comparable increases in IVDML were achieved merely by mixing bovine saliva with ground forage samples. Differences were greater than could be explained by increases due to completely digestible salivary DM. There was no significant difference between animals in relation to the saliva effect on IVDML and, except for some minor differences, similar saliva effects on IVDML were measured using either the pepsin–cellulase or rumen fluid–pepsin in vitro techniques. For both C4 and C3 grasses the magnitude of the differences were inversely related to IVDML of the feed and there was little or no difference between extrusa and feed at high digestibilities (>70%) whereas differences of more than 10 percentage units were measured on low quality grass forages. The data did not suggest that the extrusa or saliva effect on digestibility was different for C3 grasses than for C4 grasses but data on C3 grasses were limited to few species and to high digestibility samples. For legume forages there was no saliva effect when the pepsin–cellulase method was used but there was a small but significant positive effect using the rumen fluid–pepsin method. It was concluded that when samples of extrusa are analysed using in vitro techniques, predicted in vivo digestibility of the feed consumed will often be overestimated, especially for low quality grass diets. The implications of overestimating in vivo digestibility and suggestions for overcoming such errors are discussed.


ZOOTEC ◽  
2016 ◽  
Vol 36 (2) ◽  
pp. 372
Author(s):  
Ronald Rompas ◽  
B Tulung ◽  
J S Mandey ◽  
M Regar

ABSTRACT UTILIZATION OF FERMENTED WATER HYACINTH (Eichhornia crassipes) IN DUCK DIETS ON DRY MATTER AND ORGANIC MATTER DIGESTIBILITY.The study aimed to determine the use of fermented water hyacinth (Eichhornia crassipes) (FWH) in duck dietson dry matter and organic matter digestibility. This study was carried out for three weeks using 20 crossbred ducks of Mojosari and Alabio (MA), 8 weeks of age. This study was conducted using a completely randomized design with 5 treatments and 4 replications. The treatments were R0 (0% FWH), R1 (10% FWH), R2 (20% FWH), R3 (30% FWH), R4 (40% FWH). Results showed that treatments was highly significant (P<0.01) affectedto dry matter and organic matter digestibility. Honestly significant difference test (HSD) on dry matter digestibility showed that there were no significant between treatment R0 (70.85%), R1 (71.08%), R2 (70,47%) and R4 (69.47%). The R3 diet was significantly higher than R4, but was not significantlydifferentbetween R0 and R1. The HSDtest on organic matter digestibility showed that there was no significant difference between R3 (76 27) and R0 (75.30%), R1 (75.43%) and R2 (775,17%), but R3 was significantly higher than R4 (74.13%). There were no significant differencebetween  R0, R1, R2, and R4 diets. It can be concluded that fermented water hyacinth reach can be used till 30% in duck diet substituted to rice bran. Keywords:Fermented water hyacinth, Digestibility,Dry matter, Organic matter


Author(s):  
Ranjani Amarakoon ◽  
Stanislav Kráčmar ◽  
Ignác Hoza ◽  
Pavel Budinský

The aim of this study was to investigate the effect of various cooking methods on nutritional qua­li­ty by evaluating in vitro digestibility of some selected legumes (two cultivars Pisum sativum and Glycine max). Samples were soaked in 0.2% NaHCO3 for 6 hours and then cooked by normal (20, 25, 30 and 35 min) pressure (8, 10, 12 and 14 min) and microwave (8, 10, 12 and 14 min) cooking. In vitro protein and dry matter digestibility were investigated. Pressure cooking and microwave cooking are recommended after soaking with the cooking time between 8–14 mins for P. sativum (Xantos and Svit) and G. max. Based on in vitro protein digestibility of all the cooking treatments, pressure cooking is the most effective.


2013 ◽  
Vol 13 (1) ◽  
pp. 49-52
Author(s):  
Yunasri Usman ◽  
M. Nur Husin ◽  
Riantia Ratni

The supplementation peel coffee beans in the ration Aceh cattle on in vitro digestibilityABSTRACT. This research was carried out at the Laboratory of  Feed and Animal Nutrition,  Department of Animal Husbandry Faculty of Agriculture Syiah Kuala University for  73 days from December 26, 2011 to March 7, 2012. The purpose of this experiment was to find out the effects of the coffee peeling on in vitro digestibility of Aceh beef cattle. Completely Randomized Design (CRD) was applied in this experiment consisting of 4 treatments  namely levels of  coffee peeling on basal diet   (R0=0%, R1=10%, R2=20%, R3=30%). Each treatment consists of 5 replications. Parameters observed in this research were dry and organic matter digestibility. The results showed that dry matter digestibility was not significantly different (P0,05)amongst treatments  54,42%, 49,32%, 45,97%, 42,08%) for R0, R1, R2 and R3 respectively.  On the other hand, organic metter digestibility was significantly different (P0,05) in which R2 treatment was the highest and R0 treatment was the lowest (54,59%).


2016 ◽  
Vol 1 (2) ◽  
pp. 42-46
Author(s):  
Hasmawati Hasmawati ◽  
Husnaeni Husnaeni

This study aimed to test the level of dry matter and organic matter mulberry natural food and Gliricidia at Ettawa goat crossbreed (PE) males. This research was conducted in March and April 2016 in the village Ranomentaa Toari District of Kolaka. The material used is 12 PE male goats aged 4-6 months with a body weight of 10.44 ± 1.13 kg. Research compiled by complete randomized design (CRD) 3 treatments and 4 replications, P1 (100% mulberry), P2 (50% and 50% mulberry Gliricidia), P3 (100% Gliricidia). The parameters observed were dry matter intake (DMI), organic matter intake (OMI), dry matter digestibility (DMD) and organic matter digestibility (OMD). The data obtained were analyzed using ANOVA with F test (P <0.01 and P <0.05), when there is a continued effect with least significant difference test (BNT). Dry matter consumption and OMI highly significant (P <0.01), while DMD and OMD not significant (P> 0.05) as a result of treatment. DMI highest average at P3 (295.41 grams), in line with the highest OMI on P3 (271.21 grams). While the percentage DMD and OMD each ranging between 39.82 to 41.23% and from 40.55 to 43.87%. The absence of the effect of treatment on DMD and OMD caused by the degradation rate of the different natural feed caused by the content of crude fiber (CF) and lignin as well as anti-nutritive substances in the forage. Based on the results of the study concluded that the DMI and the best OMI on Gliricidia, but had no effect on DMD and OMD


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