scholarly journals Penggunaan Cairan Ekstrak Isi Gizzard dan Duodenum Ayam pada Pengukuran Kecernaan In Vitro Daun Turi (Sesbania grandiflora)

Author(s):  
Ali Mursyid Wahyu Mulyono ◽  
Sri Sukar Yani ◽  
Jizan Fahmia Al Awanis

Abstract The aim of this study was to examine the use of Gizzard Contents Extracts (GCE) and Duodenum Contents Extracts (DCE) of Chicken on In Vitro digestibility measurements of Turi Leaves (Sesbania grandiflora). The study was designed using a One-way Classification of Completely Randomized Design (CRD) with 4 treatments. Each treatment was repeated three times. Types of treatment in the form of using GCE and DCE, including: (1) control treatments (without GCE or DCE), (2) GCE, (3) DCE, and (4) a mixture of GCE and DCE. The observed variables were Coefficient of Dry Matter Digestibility (CDMD), Coefficient of Organic Matter Digestibility (COMD), and Coefficient of Soluble Protein Digestibility (CSPD). The results showed that the addition of GCE, DCE, and a mixture of GCE and DCE could not increase the CDMD, COMD, and CSPD of Turi Leaves compared to the control treatment. Keywords: Duodenal contents extract; Gizzard content extract; In vitro digestibility; Turi leaves.   Abstrak Penelitian bertujuan untuk mengkaji penggunaan cairan ekstrak isi gizzard (CEIZ) dan duodenum (CEID) ayam pada pengukuran kecernaan in vitro daun turi. Penelitian dirancang menggunakan Rancangan Acak Lengkap (RAL) pola searah dengan 4 perlakuan. Masing-masing perlakuan diulang tiga kali. Macam perlakuan berupa penggunaan penggunaan CEIZ dan CEID ayam, meliputi perlakuan kontrol (tanpa CEIZ maupun CEID), CEIZ, CEID, dan campuran CEIZ dan CEID. Variabel pengamatan berupa koefisien cerna bahan kering (KCBK), koefisien cerna bahan organic (KCBO), dan koefisien cerna protein terlarut (KCPT). Hasil penelitian menunjukkan bahwa perlakuan penambahan cairan ekstrak isi gizzard, duodenum dan campuran keduanya tidak dapat meningkatkan KCBK, KCBO, dan KCPT daun turi dibandingkan dengan perlakuan control. Kata kunci: Cairan ekstrak isi duodenum; Cairan ekstrak isi gizzard;  Daun turi; Kecernaan in vitro.

2015 ◽  
Vol 15 (1) ◽  
pp. 46-51
Author(s):  
D. D. Dinata ◽  
Widiyanto Widiyanto ◽  
Retno Iswarin Pujaningsih

(The effect of supplementation and protection of kapok seed oil on in vitro ruminal fermentability of elephant grass) ABSTRACT. The purpose of this research was to study the interaction between supplementation and protection of kapok seed oil (KSO) influence on in vitro digestibility and ruminal fermentability products of elephant grass.  Material used were elephant grass, kapok seed oil, KOH and CaCl2 reagens and cattle rumen fluids.  Completely randomized design (CRD) was used with two factorial pattern 3 x 5 with 2 replications, first factor was supplementation of KSO consist of 3 levels: 5% (S1); 10% (S2); and 15% (S3), respectively.  Second factor was protection consist of 5 levels: 0% (P0); 25% (P1); 50% (P2); 75% (P3) and 100% (P4); also control treatment without supplementation and protection of KSO (S0P0).  The result of this research showed there was no interaction between supplementation and protection of KSO on digestibility of elephant grass.  The highest In vitro Dry Matter Digestibility (IVDMD) and In vitro Digestibility of Organic Material (IVDOM) reached by control treatment, 55,17% and 54,27%, respectively.  The results of this research showed influence of interaction between supplementation and protection of KSO on VFA and NH3 production (P0.05).  The highest VFA and NH3 production reached by 10% supplementation and 25% protection were 152,5 and 6,50 mM.  The conclusion of this study showed that supplementation and protection of KSO was not effecting ruminal fermentation, but decreasing in vitro digestibility of elephant grass.


2013 ◽  
Vol 13 (1) ◽  
pp. 49-52
Author(s):  
Yunasri Usman ◽  
M. Nur Husin ◽  
Riantia Ratni

The supplementation peel coffee beans in the ration Aceh cattle on in vitro digestibilityABSTRACT. This research was carried out at the Laboratory of  Feed and Animal Nutrition,  Department of Animal Husbandry Faculty of Agriculture Syiah Kuala University for  73 days from December 26, 2011 to March 7, 2012. The purpose of this experiment was to find out the effects of the coffee peeling on in vitro digestibility of Aceh beef cattle. Completely Randomized Design (CRD) was applied in this experiment consisting of 4 treatments  namely levels of  coffee peeling on basal diet   (R0=0%, R1=10%, R2=20%, R3=30%). Each treatment consists of 5 replications. Parameters observed in this research were dry and organic matter digestibility. The results showed that dry matter digestibility was not significantly different (P0,05)amongst treatments  54,42%, 49,32%, 45,97%, 42,08%) for R0, R1, R2 and R3 respectively.  On the other hand, organic metter digestibility was significantly different (P0,05) in which R2 treatment was the highest and R0 treatment was the lowest (54,59%).


2021 ◽  
Vol 3 (2) ◽  
pp. 92
Author(s):  
Tri Ambarwati ◽  
Ali Mursyid Wahyu Mulyono ◽  
Engkus Ainul Yakin

Protein source feed is the feed that has the biggest share in the nutritional needs of livestock. Lamtoro or Leucaena leucocephala is one of the leaf flours that can be used as an alternative feed ingredient for poultry in the tropics. With its abundant availability and does not compete for its needs with humans and has no selling value so that it can minimize consumption costs for poultry. This study aims to determine the effect of fluid from the digestive tract of chickens on the in vitro digestibility measurement of lamtoro leaves. The study used a completely randomized design (CRD) with a unidirectional pattern with 4 treatments and 3 replications. The treatments consisted of P0 = control, P1 = gizzard fluid, P2 = duodenal fluid and P3 = a mixture of gizzard and duodenal fluid. The results of this study show the average Dry Matter Digestibility Coefficient (%) for the treatment P0, P1, P2 and P3 respectively; 19.276, 29.089, 20.515 and 28.737. Organic Matter Digestibility Coefficient ; 27.702, 36.313, 24.795 and 35.649. Dissolved Protein Digestibility Coefficient ; 39.777, 53.288, 21.145 and 48.737. The results of the statistical test that the administration of lamtoro leaves showed a significant difference (P<0.05) on the digestibility coefficient of dry matter, organic matter and dissolved protein. The conclusion is that the use of gizzard liquid is more effective in increasing the in vitro digestibility of lamtoro leaves.


2015 ◽  
Vol 2 (3) ◽  
pp. 17
Author(s):  
Indrayani Indrayani ◽  
Harapin Hafid ◽  
Dian Agustina

ABSTRACTThis study aims to determine the digestibility of dry matter and organic matter level waste silage mixed vegetables and Gliricidia leaves were tested in vitro. This study used a completely randomized design with 3 treatments and 3 replications. Each of these treatments is R0 (Gliricidia leaves 100%), R1 (Gliricidia leaves 70% + 30% silage vegetable waste), and R2 (Gliricidia leaves 40% + 60% silage vegetable waste). Data were analyzed using analysis of variance (ANOVA) and further testing using the test Honestly Significant Difference (HSD). The results of ANOVA showed that the mixture of vegetable waste silage was highly significant (p <0.05) on dry matter digestibility and significantly (p <0.05) on the digestibility of organic matter. It can be concluded that the mixed of vegetable waste silage and Gliricidia leaves can improved digestibility of dry matter and organic matter, treatment of 40% and 60% Gliricidia leaves plus waste vegetable produce silage dry matter digestibility and percentage of organic matter is best (72,24% and 68,19%).Keyword: Silage vegetable waste, gliricidia leaves, dry and organic matter digestibilityABSTRAKPenelitian ini bertujuan untuk mengetahui kecenaan bahan kering dan bahan organik tingkat campuran silase sampah sayur dan daun gamal yang diuji secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 3 perlakuan dan 3 ulangan. Masing-masing perlakuan tersebut ialah R0 (daun gamal 100%), R1 (daun gamal 70% + silase sampah sayur 30%), dan R2 (daun gamal 40% + silase sampah sayur 60%). Data yang diperoleh dianalisis dengan menggunakan analisis sidik ragam (ANOVA) dan uji lanjut menggunakan uji Beda Nyata Jujur (BNJ). Hasil analisis sidik ragam menunjukkan bahwa campuran silase sampah sayur berpengaruh sangat nyata (p<0,05) terhadap kecernaan bahan kering dan berpengaruh nyata (p<0,05) terhadap kecernaan bahan organik. Dapat disimpulkan bahwa perlakuan campuran silase sampah sayur dan daun gamal dapat meningkatkan kecernaan bahan kering dan bahan organik, perlakuan 40% daun gamal dan 60% silase sampah sayur menghasilkan persentase kecernaan bahan kering dan bahan organik yang terbaik yaitu (72,24% dan 68,19%).Kata kunci : Silase sampah sayur, daun gamal, kecernaan bahan kering, dan bahan organik.


Author(s):  
Ranjani Amarakoon ◽  
Stanislav Kráčmar ◽  
Ignác Hoza ◽  
Pavel Budinský

The aim of this study was to investigate the effect of various cooking methods on nutritional qua­li­ty by evaluating in vitro digestibility of some selected legumes (two cultivars Pisum sativum and Glycine max). Samples were soaked in 0.2% NaHCO3 for 6 hours and then cooked by normal (20, 25, 30 and 35 min) pressure (8, 10, 12 and 14 min) and microwave (8, 10, 12 and 14 min) cooking. In vitro protein and dry matter digestibility were investigated. Pressure cooking and microwave cooking are recommended after soaking with the cooking time between 8–14 mins for P. sativum (Xantos and Svit) and G. max. Based on in vitro protein digestibility of all the cooking treatments, pressure cooking is the most effective.


2017 ◽  
Vol 4 (4) ◽  
pp. 58-64
Author(s):  
Edgard Gonçalves Malaguez ◽  
Gabriella Valduga Dinarte ◽  
Leonardo Ereno Tadielo ◽  
Nathaly Lopes Toledo Dos Santos ◽  
Deise Dalazen Castagnara

The spring forage emptiness is an obstacle to the development of Brazilian livestock, including in the Bioma Pampa region. Feed strategies for this period contributed to the development of livestock and to the conservation of the biome. The objective of this work was to know the nutritional potential of forages during spring forage for use as strategies in the forage planning. The experiment had a completely randomized design, with ten forages and four replicates. The chemical composition, in vitro dry matter digestibility (IVDMD) and the total digestible nutrient content (NTD) of elephant grass, brachiaria cultivars Convert and Marandu, Tifton 68; diploid and tetraploid ryegrass; red, white and arrowleaf clover; and bird’s-foot trefoil. The grasses had a higher proportion of fibrous constituents, and lower levels of NDT and DMD, thus, lower nutritional value. Negative correlation between mineral matter and fibrous constituents with CP, TDN and IVDMD, was observed. Forages studied can be used as pasture alternatives for ruminants in the spring forage emptiness of the Pampa, Brazil, however, the strategy of using them should be a pasture planning.


2016 ◽  
Vol 1 (1) ◽  
pp. 808-815
Author(s):  
Sugrahadi Ahmad Aprianto ◽  
Yunasri Usman ◽  
Asril Asril

Abstrak: Penelitian ini telah dilaksanakan di Laboratorium Ilmu Nutrisi dan Makanan Ternak Program Studi Peternakan Fakultas Pertanian Universitas Syiah Kuala dan dilanjutkan dengan pengiriman sampel penelitian ke Laboratorium Ternak Perah Institut Pertanian Bogor (IPB) untuk pengujian kecernaan secara in vitro. Tujuan dari penelitian ini adalah menguji kecernaan secara in vitro complete feed berbahan dasar ampas sagu dengan teknik fermentasi yang berbeda.Pembuatan complete feed disusun sesuai dengan kebutuhan ternak domba. Penelitian ini menggunakan Rancangan Acak lengkap (RAL) dengan 3 perlakuan dan masing-masing perlakuan terdiri atas 5 ulangan. Perlakuan 1 (kontrol) adalah complete feed tanpa penambahan Saus Burger Pakan (SBP) dan tanpa fermentasi, perlakuan 2 adalah ampas sagu difermentasi dengan SBP selama 14 hari kemudian dicampur menjadi complete feed dan difermentasi hingga 21 hari, sedangkan perlakuan 3 adalah ampas sagu + complete feed kemudian difermentasi dengan SBP selama 21 hari. Parameter yang diamati dalam penelitian ini adalah pH, Bahan Kering (BK), Bahan Organik (BO), Koefisien Cerna Bahan Kering (KCBK), dan Koefisien Cerna Bahan Organik (KCBO). Hasil pengamatan menyimpulkan bahwa, teknik fermentasi yang berbeda berpengaruh sangat nyata (P0,01) terhadap semua parameter yang diamati, yaitu pH, BK, BO, KCBK dan KCBO. Dari hasil penelitian dapat disimpulkan bahwa nilai kecernaan complete feed berbahan dasar ampas sagu dipengaruhi oleh perbedaan teknik fermentasi. Digestibility Evaluation In Vitro Complete Feed Fermentation Sago Residues Based with Different Fermentation Techniques Abstract: This research was conducted at the Laboratory of Nutrition and Feed Studies, Program study Animal Husbandry, Faculty of Agriculture, University of Syiah Kuala and continued with the delivery of sample to the Laboratory of Dairy Cattle Institute Pertanian Bogor (IPB) for testing of in vitro digestibility. The purpose of this study was to test in vitro digestibility of complete feed made from  the residues of sago with different fermentation techniques. Complete feed was arranged based on the needs of sheep. This study used a complete randomized design (CRD) with 3 treatments and each treatment consisted of 5 replicates. Treatment 1 (control) was a complete feed without the addition of Saus Burger Pakan (SBP) and without fermentation, treatment 2 was sago residu  fermented with SBP for 14 days and then mixed into a complete feed and fermented up to 21 days, whereas treatment 3 was the sago residue + complete feed then fermented with SBP for 21 days. The parameters observed in this study were pH, Dry Matter (BK), Organic Matter (BO), Dry Matter digestibility coefficients (KCBK), and Organic Matter Digestibility Coefficient (KCBO). The result influence showed that, different fermentation technique was significantly (P 0.01) all parameters, namely pH, BK, BO, KCBK and KCBO. It can be concluded that the digestibility of complete feed made from sago residue influenced by differences in fermentation techniques


2020 ◽  
Vol 17 (1) ◽  
pp. 45
Author(s):  
Tri Astuti

This research aimed to study effect of Corn cob fermentation using Panerochaeta chrysosporium with adding the different sources of carbohydrat on the digestibility of dry matter, organic matter, and crude protein by in-vitro methode. A Completely Randomized Design  with 4 treatments and  4 replications. The treatments  were : P0 = fermented corncobs + without carbohydrt, P1 = fermented corncobs + 10% rice bran; P2 = fermented corncobs + tapioca flour 10%; P3 = corncob fermented + molasses 10%. The results of this study shown a very significant affected  (P <0.01) on the digestible of dry matter and organic matter but no affected (P> 0.05) on crude protein digestibility. The best results  digestibility of dry matter (66.98%) on the  P3 treatment adding  molasses, and  the highest digestibility of organic matter (67.49%) on the P3 treatment too, but the highest digestibility of  crude protein (51.37%)  in P1 treatment (adding by rice bran)


2006 ◽  
Vol 12 (2) ◽  
pp. 193
Author(s):  
Jesús Fuentes ◽  
Antonio Cruz ◽  
Lorenzo Castro ◽  
Gilberto Gloria ◽  
Sergio Rodríguez ◽  
...  

This research was conducted to evaluate 16 corn varieties cultivated for silage. The evaluation included: fodder production of green and dry matter, protein content and in vitro dry matter digestibility (IVDMD) and organic matter digestibility (IVOMD). A completely randomized design was used for statistical analysis and Tukey mean separation was used when appropiated. Fodder production as kg/ha was higher for AN446 (114028), AN445 (110993), and AN430R (106389). Dry matter fodder production was higher for AN447 (29270), AN461 (28026), VS373 (25434). Protein content (%) was higher for G4657 (7.58), AN388 (7.28), and AN448 (7.17). The best values for IVDMD (%)were for G1990 (69.14), AN446 (63.78), and AN430RR (63.50), while the higher values for IVOMD (%) were for G1990 (71.78), AN446 (65.20), and AN447 (65.12). Varieties AN446 and AN447 appeared frequently with the best values in the parameters evaluated, therefore, new research has to be performed with these varieties to confirm results found in this study.


2016 ◽  
Vol 21 (1) ◽  
pp. 9
Author(s):  
Dwi Yulistiani ◽  
Z. A. Jelan ◽  
J. B. Liang

<p class="abstrak2">This experiment was carried out to determine the effect of mulberry-leucaena foliage mixed feed on protein digestibility and VFA production using an in vitro gas production study. Mulberry was mixed with one of 2 leucaena varieties (<em>Leucaena leucocephala</em> hybrid and <em>Leucaena leucocephala</em> local) at 3 levels (0, 25 and 50%). Study was conducted in completely randomized design. Mulberry foliage, leucaena and mixtures of mulberry-leucaena were incubated for 24 hours in glass syringes. Parameter recorded were gas production, in vitro true dry matter digestibility (IVTDMD), in vitro N digestibility (IVND) and VFA production. Results of the study showed that supplementation of leucaena to mulberry decreased IVOMD, gas production and protein digestibility in the rumen buffered medium. The mixture of mulberry and leucaena hybrid at ratio 50% resulted in the lowest IVND than other treatment. However it increased protein digestibility in acid pepsin incubation as an estimate of protein availability in intestine. Gas production decreased in mulberry leucaena mixtures was followed by decreased total VFA production. Tannin derived from leucaena hybrid supplementation to mulberry at ratio 1 : 1 was most effective to decrease protein digestion in the rumen and to increase protein digestibility in acid pepsin incubation. In conclusion mixing of hybrid leucaena hybrid with mulberry foliage was able to protect protein degradation from mulberry in the rumen.</p>


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