Electrospun polymeric dressings functionalized with antimicrobial peptides and collagen type I for enhanced wound healing

Acupuncture regulates inflammation process and growth factors by increasing blood circulation in affected areas. In this study, we examined whether acupuncture has an effect on wound healing in injured rat. Rats were assigned randomly into two groups: control group and acupuncture group. Acupuncture treatment was carried out at 8 sites around the wounded area. We analyzed the wound area, inflammatory cytokines, proliferation of resident cells, and angiogenesis and induction of extracelluar matrix remodeling. At 7 days after-wounding the wound size in acupuncture-treat group was decreased more significantly compared to control group. In addition, the protein levels of proinflammatory cytokines such as tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) were significantly decreased compared to the control at 2 and 7 days post-wounding. Also, we analyzed newly generated cells by performing immunostaining for PCNA and using several phenotype markers such as CD-31,α-SMA, and collagen type I. In acupuncture-treated group, PCNA-positive cell was increased and PCNA labeled CD-31-positive vessels,α-SMA- and collagen type I-positive fibroblastic cells, were increased compared to the control group at 7 days post-wounding. These results suggest that acupuncture may improve wound healing through decreasing pro-inflammatory response, increasing cell proliferation and angiogenesis, and inducing extracellular matrix remodeling.

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Electrospun poly(vinyl) alcohol/collagen nanofibrous scaffold hybridized by graphene oxide for accelerated wound healing

The International Journal of Artificial Organs ◽

10.1177/0391398818775949 ◽

2018 ◽

Vol 41 (8) ◽

pp. 467-473 ◽

Cited By ~ 9

Author(s):

Rethinam Senthil ◽

Robert Berly ◽

Thimmiah Bhargavi Ram ◽

Nallathambi Gobi

Keyword(s):

Wound Healing ◽

Graphene Oxide ◽

Vinyl Alcohol ◽

Collagen Type ◽

Collagen Type I ◽

Fish Bone ◽

Poly Vinyl Alcohol ◽

Type I

Purpose: In this study, a blend of synthetic polymer (poly(vinyl) alcohol), natural polymer (collagen type I from fish bone), and graphene oxide nanoparticles is used to fabricate a composite nanofibrous scaffold, by electrospinning, for their potential application in accelerated wound healing. Methods: The scaffold was characterized for its physicochemical and mechanical properties. In vitro studies were carried out using human keratinocyte cell line (HaCaT) which proved the biocompatibility of the scaffold. In vivo study using mice model was carried out and the healing pattern was evaluated using histopathological studies. Results: Scaffold prepared from poly(vinyl) alcohol, collagen type I from fish bone, and graphene oxide possessed better physicochemical and mechanical properties. In addition, in vivo and in vitro studies showed its accelerated wound healing properties. Conclusion: The scaffold with required strength and biocompatibility may be tried as a wound dressing material in large animals after getting necessary approval.

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Elastomeric Cardiowrap Scaffolds Functionalized with Mesenchymal Stem Cells-Derived Exosomes Induce a Positive Modulation in the Inflammatory and Wound Healing Response of Mesenchymal Stem Cell and Macrophage

Biomedicines ◽

10.3390/biomedicines9070824 ◽

2021 ◽

Vol 9 (7) ◽

pp. 824

Author(s):

Juan Carlos Chachques ◽

Chiara Gardin ◽

Nermine Lila ◽

Letizia Ferroni ◽

Veronique Migonney ◽

...

Keyword(s):

Stem Cells ◽

Wound Healing ◽

Mesenchymal Stem Cells ◽

Collagen Type ◽

Cell Function ◽

Macrophage Polarization ◽

Collagen Type I ◽

Type I ◽

Wound Healing Response ◽

Biological Tool

A challenge in contractile restoration of myocardial scars is one of the principal aims in cardiovascular surgery. Recently, a new potent biological tool used within healing processes is represented by exosomes derived from mesenchymal stem cells (MSCs). These cells are the well-known extracellular nanovesicles released from cells to facilitate cell function and communication. In this work, a combination of elastomeric membranes and exosomes was obtained and tested as a bioimplant. Mesenchymal stem cells (MSCs) and macrophages were seeded into the scaffold (polycaprolactone) and filled with exosomes derived from MSCs. Cells were tested for proliferation with an MTT test, and for wound healing properties and macrophage polarization by gene expression. Moreover, morphological analyses of their ability to colonize the scaffolds surfaces have been further evaluated. Results confirm that exosomes were easily entrapped onto the surface of the elastomeric scaffolds, increasing the wound healing properties and collagen type I and vitronectin of the MSC, and improving the M2 phenotype of the macrophages, mainly thanks to the increase in miRNA124 and decrease in miRNA 125. We can conclude that the enrichment of elastomeric scaffolds functionalized with exosomes is as an effective strategy to improve myocardial regeneration.

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Modulation of Extracellular Matrix by Scrophularia striata Extract in Vitro: A Potential Antiscarring Agent

Jundishapur Journal of Natural Pharmaceutical Products ◽

10.5812/jjnpp.95301 ◽

2020 ◽

Vol 15 (3) ◽

Author(s):

Pari Tamri ◽

Rasool Haddadi ◽

Fatemeh Javani Jouni

Keyword(s):

Extracellular Matrix ◽

Wound Healing ◽

Human Skin ◽

Collagen Type ◽

Collagen Type I ◽

Collagen Content ◽

Type I ◽

Hypertrophic Scars ◽

Total Collagen ◽

Scrophularia Striata

Background: Hypertrophic scars are the consequences of the aberration of normal wound healing. To date, therapeutic strategies for abnormal scarring have been unsuccessful. ‎‏The abnormal extracellular‎ matrix is one of the most important contributing factors to ‎hypertrophic scars. ‏Scrophularia striata has been used in Iranian folk medicine for the treatment of burn wounds. ‏The plant extract accelerates wound healing and attenuates scar formation. Objectives: The study was performed to investigate the effects of Scrophularia striata hydroalcoholic extract (SSE) on MMP1, MMP8, fibronectin, collagen type I, and total collagen produced by human skin fibroblasts in the culture medium. Methods: The effects of SSE on the expression of MMP1, MMP8, fibronectin, and collagen type I in human skin fibroblast (HSF) were evaluated using Q-PCR and Western blotting methods. In addition, the effect of SSE on the total collagen content was measured in cultured HSF using Red Sirius Kit. Results: SSE significantly induced the expression of MMP1 and suppressed the production of fibronectin at the mRNA and protein levels. The total collagen content was significantly lower in SSE-treated cells than in untreated cells. SSE did not have any significant effect on MMP8 and collagen type I expression. Conclusions: The results of this study revealed that SSE could modulate the extracellular matrix turnover and had the potential for the prevention and treatment of hypertrophic scars.

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