Routine Procedures for the Isolation and Identification of Certain Pathogenic Fungi

1943 ◽  
Vol 13 (ts7) ◽  
pp. 123-127
Author(s):  
Henry J. Peppler ◽  
Sanitary Corps
Keyword(s):  
Pathogenic Fungi ◽  
Isolation And Identification ◽  
Routine Procedures

Isolation and Identification of the Main Pathogenic Fungi from Postharvest Hami Melon in Later Period of Storage

2011 ◽  
Vol 343-344 ◽  
pp. 1212-1216 ◽  
Author(s):  
Cun Kun Chen ◽  
Wen Sheng Wang ◽  
Jia Ning ◽  
Yuan Hui Gao
Keyword(s):  
Bacterial Strain ◽  
Pathogenic Fungi ◽  
Isolation And Identification ◽  
Koch’S Postulates ◽  
Penicillium Sp ◽  
Alternaria Sp ◽  
Fusarium Sp ◽  
Koch's Postulates

In order to investigate the reasons for the occurrence of melon disease, according to Koch’s postulates, the pathogenic fungi which caused the disease of postharvest melon“86-1”has been isolated and three kinds of bacterial strain has been identified. By identifying the pathogenicity, results revealed that they are Alternaria sp., Fusarium sp. and Penicillium sp.. They are the major pathogenic fungi which caused the decay of the postharvest Hami melon.

scholarly journals Isolation and Identification of Antibiotic-Producing Halophilic Bacteria from Dagh Biarjmand and Haj Aligholi Salt Deserts, Iran

2019 ◽  
Vol 25 (1) ◽  
pp. 70-77 ◽  
Author(s):  
Babak Elyasifar ◽  
Sevda Jafari ◽  
Somayeh Hallaj-Nezhadi ◽  
Florence Chapeland-leclerc ◽  
Gwenaël Ruprich-Robert ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Plant Pathogens ◽  
Halophilic Bacteria ◽  
Pathogenic Fungi ◽  
Antibacterial Activities ◽  
Biochemical Properties ◽  
Diffusion Method ◽  
Human Pathogens ◽  
Isolation And Identification ◽  
Phylogenic Tree

Background: Halophilic bacteria are potent organisms in production of novel bioactive antimicrobial compounds which might be considered in drug innovation and control of plant pathogens. Salt deserts in Semnan province are of the most permanent hypersaline areas in the North of Iran. Despite the importance of these areas, there is no scientific report regarding the biodiversity and potency of their halophilic bacteria. Thus, aforementioned areas were selected to detect the halophilic bacteria. Methods: Here, seven strains were isolated and cultured on their molecular and biochemical properties were characterized. To determine the antibiotic potency of the isolates, agar well diffusion method was conducted. Phylogenetic analysis was done to reveal the isolates relationship with previously known strains. Results: As a result, growth of the strains in the medium containing 5 to 20% (w/v) NaCl determined that the majority of the isolates were moderately halophile. Catalase activity of all strains was positive. The results represented that D6A, Dar and D8B have antimicrobial effects against different plant and human pathogens. Phylogenic tree analysis also showed that two strains of D6A and Dar are belonged to Bacillus subtilis and D8B is belonged to Virgibacillus olivae. The bacteria extracts were evaluated for their antifungal and antibacterial activities on human and Plant pathogenic strains. The MIC of the extract B. subtilis against was found active against human pathogenic fungi and Plant pathogenic bacteria and fungi, ranging from 12.5 to 25 µg/mL. Conclusion: This study highlights the therapeutic and prophylactic potential of B. subtilis extracts as antibacterial and antifungal agents.

scholarly journals Isolation and Identification of Endophytic Bacteria from Mycorrhizal Tissues of Terrestrial Orchids from Southern Chile

Diversity ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 55 ◽  
Author(s):  
Héctor Herrera ◽  
Tedy Sanhueza ◽  
Alžběta Novotná ◽  
Trevor C. Charles ◽  
Cesar Arriagada
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Developmental Stages ◽  
Pathogenic Fungi ◽  
Southern Chile ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Acetic Acid Production ◽  
Terrestrial Orchids ◽  
Plant Growth Promoting

Endophytic bacteria are relevant symbionts that contribute to plant growth and development. However, the diversity of bacteria associated with the roots of terrestrial orchids colonizing Andean ecosystems is limited. This study identifies and examines the capabilities of endophytic bacteria associated with peloton-containing roots of six terrestrial orchid species from southern Chile. To achieve our goals, we placed superficially disinfected root fragments harboring pelotons on oatmeal agar (OMA) with no antibiotic addition and cultured them until the bacteria appeared. Subsequently, they were purified and identified using molecular tools and examined for plant growth metabolites production and antifungal activity. In total, 168 bacterial strains were isolated and assigned to 8 OTUs. The orders Pseudomonadales, Burkholderiales, and Xanthomonadales of phylum Proteobacteria were the most frequent. The orders Bacillales and Flavobacteriales of the phylla Firmicutes and Bacteroidetes were also obtained. Phosphate solubilization was detected in majority of isolates; however, it was significantly higher in Collimonas pratensis and Chryseobacterium sp. (PSI = 1.505 ± 0.09 and 1.405 ± 0.24, respectively). Siderophore production was recorded only for C. pratensis (0.657 ± 0.14 mm day−1), Dyella marensis (0.131 ± 0.02 mm day−1), and Luteibacter rhizovicinus (0.343 ± 0.12 mm day−1). Indole acetic acid production was highly influenced by the isolate identity; however, the significantly higher activity was recorded for Pseudomonas spp. (ranging from 5.507 ± 1.57 µg mL−1 to 7.437 ± 0.99 µg mL−1). Additionally, six bacterial isolates were able to inhibit the growth of some potential plant pathogenic fungi. Our findings demonstrate the potential for plant growth promoting capabilities and some antifungal activities of endophytic bacteria inhabiting the mycorrhizal tissue of terrestrial orchids, which may contribute especially at early developmental stages of orchid seedlings.

Isolation and Identification of Fungal Pathogen Associated with Post Harvest Deterioration of Cucumber (Cucumis sativus L.) Fruits in Three Selected Markets in Jos, Nigeria

2019 ◽  
pp. 1-8
Author(s):  
M. Ishaya ◽  
A. E. Anzaku ◽  
W. C. John ◽  
N. Janfa ◽  
O. Oke ◽  
...  
Keyword(s):  
Fungal Pathogens ◽  
Sampling Technique ◽  
Pathogenic Fungi ◽  
Geotrichum Candidum ◽  
Isolation And Identification ◽  
Cucumis Sativus L ◽  
Post Harvest ◽  
Fungal Isolates ◽  
Cucumber Fruit ◽  
Fruit Samples

Aim: This study was carried out to isolate, identify and characterize fungal pathogens associated with post-harvest spoilage of cucumber. Study Design: This research study was done using random sampling technique.  Place and Duration of Study: The experiment was carried out from March to June, 2019 at biology laboratory Federal College of Forestry, Jos. Methodology: Fungi isolates were obtained from diseased portions of the cucumber fruit samples cultured on PDA media and incubated at 28ºC2 for seven days. The mycelial were identified macroscopically and microscopically. Pathogenicity tests were conducted for all the fungal pathogens identified by inoculating healthy cucumber samples and incubating for ten days with the readings being taken at two day intervals. The data obtained were analyzed using ANOVA and means were separated using LSD at P ≤ 0.05. Results: Aspergillus fumigatus, Fusarium sp., Geotrichum candidum and Yeast sp were isolated and identified. Result showed that Gada biu market was the most heavily infested location with all the fungal isolates. Geotrichum candidum constituted the highest (50%) occurrence of fungal isolates from all locations. All the fungal isolates were found to be pathogenic on cucumber fruits, with Fusarium being the most destructive, followed by yeast and Geotrichum candidum and the least was Aspergilus fumigatus. Conclusion: The results obtained in this study showed the isolates identified were involved in Cucumber spoilage. Therefore, Careful handling of Cucumber fruits should be ensured to prevent the spread of these pathogenic fungi.

scholarly journals Isolation and Identification of Endophytic Fungi on Cocoa Plant (Theobroma cacao L.) and Antagonist Activity against Colletotrichum gloeosporioides

2021 ◽  
Vol 10 (9) ◽  
pp. 419-430
Author(s):  
Zaedar A. Rosmana ◽  
T. Kuswinanti Nasaruddin
Keyword(s):  
Endophytic Fungi ◽  
Theobroma Cacao ◽  
Colletotrichum Gloeosporioides ◽  
Pathogenic Fungi ◽  
Isolation And Identification ◽  
Trichoderma Sp ◽  
Cocoa Production ◽  
Young Leaves ◽  
Central Sulawesi ◽  
Theobroma Cacao L

Anthracnose disease which is caused by Colletotrichum gloeosporioides fungi becomes a resistor factor in cocoa production. Infect on young leaves creates leaf blight, consequently those leaves can die completely or only several parts starting from leaf blade and then fall, but infection at mature leaves spots appear in random border form and leave hole in the same location. Branches with infected leaves will experience die back and look like a broom, in the other side, infection at young fruit turns fruit to be dried and wrinkled while at mature fruits showed dried and rotten tips. This research is aimed at finding potential endophytic fungi from cocoa plant that can resist the growth of pathogenic fungi, C. gloeosporioides which cause anthracnose on cocoa plant (Theobroma cacao L). Isolation and identification of endophytic fungi on cocoa plant from Banggai district in Central Sulawesi results showed 10 isolates of endopphytic fungi: 2 isolates from leaves organ isolation; 4 isolates from stem; and 4 isolates from fruit pod. They both identified morphologically and macroscopically and found to have similarity to Trichoderma sp, Penicillium sp, Aspergilus sp that has resistance capability against Colletotrichum gloeosporioides colony through antagonist mechanism marked by the presence of resistance zone. Trichodermasp demonstrated the highest percentage at isolate G in 56,92%.

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