scholarly journals Routine molecular subgrouping of medulloblastoma: Bridging the divide between research and the clinic using low-cost, mass spectrometry-based DNA methylomics

2016 ◽  
Vol 27 ◽  
pp. vi103
Author(s):  
E. Schwalbe ◽  
D. Hicks ◽  
G. Rafiee ◽  
M. Bashton ◽  
H. Gohlke ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Low Cost

scholarly journals A low-cost mass spectrometry-based approach for quantifying purines in placental explants

2021 ◽  
Vol 460 ◽  
pp. 116490
Author(s):  
Ruslan Rodriguez ◽  
Igor Konovets ◽  
Serhii Ralchenko ◽  
Maxsim Kharkhota ◽  
Andrij Kostyuk ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Low Cost

scholarly journals MALDIViz: A Comprehensive Informatics Tool for MALDI-MS Data Visualization and Analysis

2017 ◽  
Vol 22 (10) ◽  
pp. 1246-1252 ◽  
Author(s):  
Kishore Kumar Jagadeesan ◽  
Simon Ekström
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
High Throughput Screening ◽  
Web Application ◽  
Low Cost ◽  
Maldi Ms ◽  
Ionization Mass ◽  
Label Free ◽  
Label Free Detection ◽  
R Shiny

Recently, mass spectrometry (MS) has emerged as an important tool for high-throughput screening (HTS) providing a direct and label-free detection method, complementing traditional fluorescent and colorimetric methodologies. Among the various MS techniques used for HTS, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) provides many of the characteristics required for high-throughput analyses, such as low cost, speed, and automation. However, visualization and analysis of the large datasets generated by HTS MALDI-MS can pose significant challenges, especially for multiparametric experiments. The datasets can be generated fast, and the complexity of the experimental data (e.g., screening many different sorbent phases, the sorbent mass, and the load, wash, and elution conditions) makes manual data analysis difficult. To address these challenges, a comprehensive informatics tool called MALDIViz was developed. This tool is an R-Shiny-based web application, accessible independently of the operating system and without the need to install any program locally. It has been designed to facilitate easy analysis and visualization of MALDI-MS datasets, comparison of multiplex experiments, and export of the analysis results to high-quality images.

A test apparatus for secondary ion mass spectrometry

1983 ◽  
Vol 61 (4) ◽  
pp. 535-542 ◽  
Author(s):  
N. Klaus ◽  
J. D. Brown
Keyword(s):  
Mass Spectrometry ◽  
Secondary Ion Mass Spectrometry ◽  
Low Cost ◽  
Ion Source ◽  
Channel Electron Multiplier ◽  
Electron Multiplier ◽  
Spherical Source ◽  
Ion Mass Spectrometry ◽  
Cylindrical Source ◽  
Secondary Ion

A low cost test device for secondary ion mass spectrometry (SIMS) components is described. It consists of a turbomolecular pumped vessel containing an insulated sample stage on an x−y manipulator, extraction optics, quadrupole mass filter, and channel electron multiplier. The construction and characteristics of a cylindrical and a spherical saddlefield ion source are described. The output of the cylindrical source is 10−4 A cm−2 whereas that of the spherical source is in the order of 10−3 A cm−2 at voltages up to 9 kV and at a beam divergence of 4°.

Direct Comparison of Cavity Ring Down Spectrometry and Isotope Ratio Mass Spectrometry for Detection of Sugar Adulteration in Honey Samples

2018 ◽  
Vol 101 (6) ◽  
pp. 1857-1863 ◽  
Author(s):  
Madhavi Mantha ◽  
John R Urban ◽  
William A Mark ◽  
Anatoly Chernyshev ◽  
Kevin M Kubachka
Keyword(s):  
Mass Spectrometry ◽  
Isotope Ratio ◽  
Low Cost ◽  
Isotope Ratio Mass Spectrometry ◽  
Cane Sugar ◽  
Technical Knowledge ◽  
Scientific Methods ◽  
High Fructose ◽  
Honey Analysis ◽  
Cavity Ring Down

Abstract In the last several years, economically motivated adulteration (EMA) of foods including honey has received increased attention. The addition of inexpensive sweeteners such as high fructose corn syrup or cane sugar to honey is still encountered despite scientific methods that can routinely detect this type of adulteration. The standard method for detection of these adulterants utilizes isotope ratio mass spectrometry (IRMS); however, this technique requires an elevated degree of technical knowledge for operation as well as a high cost for purchase and maintenance. Cavity ring down spectroscopy (CRDS) has demonstrated potential for this type of analysis and is less expensive with simpler operation. This study evaluates CRDS for the detection of low-cost sweeteners added to honey and compares the performance of CRDS to IRMS. Several honey samples were analyzed, and the advantages and limitations specific to CRDS were evaluated. Overall, the results indicate that CRDS provides a performance comparable to the benchmark technique IRMS for EMA honey analysis.

scholarly journals An Efficient and Green Synthesis of Fluorine Containing Benzo[a]xanthen-11(12H)-ones and Evaluation of their Anticancer Activity

2020 ◽  
Vol 32 (4) ◽  
pp. 923-929
Author(s):  
J. Sumalatha ◽  
A. Sreedevi ◽  
C. Radha Rani
Keyword(s):  
Mass Spectrometry ◽  
Reaction Time ◽  
Anticancer Activity ◽  
Low Cost ◽  
The Other ◽  
Significant Activity ◽  
One Pot ◽  
Efficient Procedure ◽  
Solvent Free Conditions ◽  
One Pot Condensation

A facile and an efficient procedure has been developed by one-pot condensation of naphthalene-diols, fluoro substituted benzaldehydes and cyclic 1,3-dicarbonyl compounds for the synthesis of fluorine containing benzo[a]xanthen-11(12H)-ones under the catalyst and solvent-free conditions. All the synthesized compounds were characterized by IR, NMR (1H & 13C) and mass spectrometry. Several advantages offer the present approach, such as, shorter reaction time, low cost, anticipation of toxic solvents and catalyst, higher yield of products and durability of substrate range. On the other hand, anticancer activity was also performed for the title compounds which demonstrated a significant activity on selected cancer cell lines.

scholarly journals Electrochemical (Bio)Sensors for Pesticides Detection Using Screen-Printed Electrodes

Biosensors ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 32 ◽  
Author(s):  
Beatriz Pérez-Fernández ◽  
Agustín Costa-García ◽  
Alfredo de la Escosura- Muñiz
Keyword(s):  
Mass Spectrometry ◽  
High Performance ◽  
Point Of Care ◽  
Low Cost ◽  
Review Article ◽  
Food Control ◽  
Food Contaminants ◽  
Screen Printed Electrodes ◽  
Analytical Tools ◽  
Screen Printed

Pesticides are among the most important contaminants in food, leading to important global health problems. While conventional techniques such as high-performance liquid chromatography (HPLC) and mass spectrometry (MS) have traditionally been utilized for the detection of such food contaminants, they are relatively expensive, time-consuming and labor intensive, limiting their use for point-of-care (POC) applications. Electrochemical (bio)sensors are emerging devices meeting such expectations, since they represent reliable, simple, cheap, portable, selective and easy to use analytical tools that can be used outside the laboratories by non-specialized personnel. Screen-printed electrodes (SPEs) stand out from the variety of transducers used in electrochemical (bio)sensing because of their small size, high integration, low cost and ability to measure in few microliters of sample. In this context, in this review article, we summarize and discuss about the use of SPEs as analytical tools in the development of (bio)sensors for pesticides of interest for food control. Finally, aspects related to the analytical performance of the developed (bio)sensors together with prospects for future improvements are discussed.

Electrospray Mass Spectrometry as a Technique for Elemental Analysis: Preliminary Results

1992 ◽  
Vol 46 (3) ◽  
pp. 401-406 ◽  
Author(s):  
George R. Agnes ◽  
Gary Horlick
Keyword(s):  
Mass Spectrometry ◽  
Elemental Analysis ◽  
Alkali Metals ◽  
Electrospray Mass Spectrometry ◽  
Low Cost ◽  
Ion Source ◽  
Preliminary Results ◽  
Background Ions ◽  
Elemental Mass ◽  
Elemental Mass Spectrometry

Preliminary results for the analytical use of electrospray mass spectrometry (ES-MS) for elemental analysis are presented. Spectra with the declustered plus-one ion (M+) as the dominant species have been measured for the alkali metals (Li, Na, K, Rb, and Cs) and for several transition metals (Cr, Mn, Fe, Co, Ni, Cu, Zn, Ag, and Cd) in both aqueous and methanol solvents. A number of background ions are also observed including MH+, MO+, MOH+, and MOH(H2O)+. For vanadyl sulfate and uranyl nitrate, ES conditions can be adjusted to produce VO+ and UO2+ as the dominant ions in the mass spectra, indicating that direct speciation of inorganic solution components is possible. On the basis of these preliminary results, it appears that electrospray may offer a low-cost and simple generic ion source for elemental mass spectrometry.

scholarly journals Feasibility Of Establishing An Enzyme-Linked Immunosorbent Assay (ELISA) Laboratory For The Detection Of Dioxins In Economically Marginalized And Developed Countries

2021 ◽  
Author(s):  
Adrienne K. Lee
Keyword(s):  
Mass Spectrometry ◽  
Technology Transfer ◽  
Contaminated Soil ◽  
Low Cost ◽  
Health And Safety ◽  
Developed Countries ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sample Analysis ◽  
Economically Marginalized ◽  
Accessible Form

Finding a low cost and accessible means of detecting for dioxins in contaminated soil is a necessary step to ensure the health and safety of humans and the environment worldwide. Conventional technologies based on mass spectrometry are expensive and inaccessible. A minimally resourced laboratory and the use of ELISA will be discussed as a feasible, accessible, low cost alternative. The correlation between a minimally resourced laboratory (Ryerson University) and a fully resourced laboratory (Ontario Ministry of the Environment) was strong (n=13, r²=0.888, slope=0.87). To demonstrate the functionality of the minimally resourced laboratory, a supplemental site was characterized using ELISA. Results from the Ryerson and OMOE laboratories produced similar dioxin concentrations of undetectable to 120.26pgTEQg⁻¹ and 32.38 to 163.2pgTEQg⁻¹, respectively. This study illustrates an alternative for evaluating contaminated soil that could serve as a technology transfer for marginalized economies, and provide an accessible form of sample analysis in developed countries.

scholarly journals Immunohistochemical typing of amyloid in fixed paraffin-embedded samples by an automatic procedure: Comparison with immunofluorescence data on fresh-frozen tissue

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0256306
Author(s):  
Antonella Barreca ◽  
Emanuel Bottasso ◽  
Francesca Veneziano ◽  
Manuela Giarin ◽  
Alberto Nocifora ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gold Standard ◽  
Retrospective Cohort ◽  
Low Cost ◽  
Amorphous Material ◽  
Immunogold Labeling ◽  
Frozen Tissue ◽  
Fresh Frozen ◽  
Antibody Panel ◽  
Automated Platform

Amyloidosis comprises a spectrum of disorders characterized by the extracellular deposition of amorphous material, originating from an abnormal serum protein. The typing of amyloid into its many variants represents a pivotal step for a correct patient management. Several methods are currently used, including mass spectrometry, immunofluorescence, immunohistochemistry, and immunogold labeling. The aim of the present study was to investigate the accuracy and reliability of immunohistochemistry by means of a recently developed amyloid antibody panel applicable on fixed paraffin-embedded tissues in an automated platform. Patients with clinically and pathologically proven amyloidosis were divided into two cohorts: a pilot one, which included selected amyloidosis cases from 2009 to 2018, and a retrospective one (comprising all consecutive amyloidosis cases analyzed between November 2018 and May 2020). The above-referred panel of antibodies for amyloid classification was tested in all cases using an automated immunohistochemistry platform. When fresh-frozen material was available, immunofluorescence was also performed. Among 130 patients, a total of 143 samples from different organs was investigated. They corresponded to 51 patients from the pilot cohort and 79 ones from the retrospective cohort. In 82 cases (63%), fresh-frozen tissue was tested by immunofluorescence, serving to define amyloid subtype only in 30 of them (36.6%). On the contrary, the automated immunohistochemistry procedure using the above-referred new antibodies allowed to establish the amyloid type in all 130 cases (100%). These included: ALλ (n = 60, 46.2%), ATTR (n = 29, 22.3%), AA (n = 19, 14.6%), ALκ (n = 18, 13.8%), ALys (n = 2, 1.5%), and Aβ2M amyloidosis (n = 2, 1.5%). The present immunohistochemistry antibody panel represents a sensitive, reliable, fast, and low-cost method for amyloid typing. Since immunohistochemistry is available in most pathology laboratories, it may become the new gold standard for amyloidosis classification, either used alone or combined with mass spectrometry in selected cases.

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