Luteinizing Hormone Stimulates CYP17A1 mRNA Expression and Androgen Production in Bovine Theca Cells via Activation of the PI3K/Akt Pathway.

2009 ◽  
Vol 81 (Suppl_1) ◽  
pp. 524-524
Author(s):  
Shin Fukuda ◽  
Makoto Orisaka ◽  
Kimihisa Tajima ◽  
Fumikazu Kotsuji
Keyword(s):  
Luteinizing Hormone ◽  
Mrna Expression ◽  
Akt Pathway ◽  
Theca Cells

GnRH-1, GnIH mRNA and Luteinizing Hormone in Domestic hens (Gallus gallus domesticus) Exposed to Different Wavelengths of light

2017 ◽  
Vol 6 (8) ◽  
pp. 5446
Author(s):  
Reddy I.J. ◽  
Ashish Mishra ◽  
Mondal S.
Keyword(s):  
Luteinizing Hormone ◽  
Mrna Expression ◽  
Egg Production ◽  
Experimental Period ◽  
Treated Group ◽  
Lower Number ◽  
Gallus Gallus Domesticus ◽  
Blood Samples ◽  
Mrna Concentration ◽  
Normal Spectrum

The objective of this study was to establish the effects of red spectrum of light (650nm, treated n=12) and normal spectrum of light (450nm control=12) on GnRH-I and GnIH mRNA expression, amplitude and frequency of luteinizing hormone (LH) and egg production from 42 to 52 weeks of age in white leghorn hens. Blood samples were collected at weekly interval from both the groups. At the 47th week of age blood samples from both the groups were collected at every 3 h for 36h to study the pulsatile secretion of LH surges. GnRH and GnIH mRNA expression pattern was studied between control and treated birds. Egg production and pause days were calculated between the two groups. LH concentration in the plasma was increased significantly (P<0.01) in hens exposed to red spectrum of light. Plasma LH concentration was higher (P<0.01) in treated birds with more number of LH surges. The amplitude and frequencies of LH were advanced in birds exposed to red spectrum of light during 36 h of sampling at 3h intervals. GnRH-I mRNA concentration was significantly (P<0.01) higher, whereas GnIH mRNA was significantly (P<0.01) lower in birds exposed to red spectrum of light compared to controls. It is hypothesized that exposure of birds to red spectrum of light enhanced (P<0.01) GnRH-I mRNA, along with LH required for ovulation and egg lay. During 77 days (42-52 weeks of age) of the experimental period, egg production was increased (p<0.01) with lower incidence of pause days in the treated group. It is concluded that low GnIH mRNA and higher levels of GnRH-I mRNA, LH, lower number of pause days enabled the birds to lay more eggs by stimulating GnRH through red spectrum of light.

scholarly journals Structural Characterization and Antioxidant Activity of Polysaccharides from Athyrium multidentatum (Doll.) Ching in d-Galactose-Induced Aging Mice via PI3K/AKT Pathway

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3364 ◽  
Author(s):  
Liang Jing ◽  
Jing-Ru Jiang ◽  
Dong-Mei Liu ◽  
Ji-Wen Sheng ◽  
Wei-Fen Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Molecular Weight ◽  
Protein Expression ◽  
Mrna Expression ◽  
Caspase 3 ◽  
Akt Pathway ◽  
Protective Mechanism ◽  
Related Factor ◽  
Bax Protein ◽  
Gene And Protein Expression

The purpose of this study was to characterize the polysaccharides from Athyrium multidentatum (Doll.) Ching (AMC) rhizome and explore the protective mechanism against d-galactose-induced oxidative stress in aging mice. Methods: A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and 1H nuclear magnetic resonance (1H NMR) spectroscopy were carried out to characterize AMC polysaccharides. The mechanism was investigated exploring d-galactose-induced aging mouse model. Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays were performed to assess the gene and protein expression in liver. Key findings: Our results showed that AMC polysaccharides were mainly composed of mannose (Man), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galactose (Gal), arabinose (Ara), and fucose (Fuc) in a molar ratio of 0.077:0.088:0.09:1:0.375:0.354:0.04 with a molecular weight of 33203 Da (Mw). AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression. Conclusion: AMC polysaccharides attenuated d-galactose-induced oxidative stress and cell apoptosis by activating the PI3K/AKT pathway, which might in part contributed to their anti-aging activity.

scholarly journals Melatonin Stimulates STAR Expression and Progesterone Production via Activation of the PI3K/AKT Pathway in Bovine Theca Cells

2019 ◽  
Vol 15 (2) ◽  
pp. 404-415 ◽  
Author(s):  
Xiaomei Wang ◽  
Kai Meng ◽  
Yuanyuan He ◽  
Hengqin Wang ◽  
Yong Zhang ◽  
...  
Keyword(s):  
Akt Pathway ◽  
Theca Cells ◽  
Progesterone Production

Steroidogenesis by avian ovarian cells: effects of luteinizing hormone and substrate availability

1983 ◽  
Vol 244 (5) ◽  
pp. E487-E493 ◽  
Author(s):  
B. L. Marrone ◽  
F. Hertelendy
Keyword(s):  
Luteinizing Hormone ◽  
Granulosa Cells ◽  
Synergistic Effects ◽  
Theca Cells ◽  
Ovarian Cells ◽  
P Accumulation ◽  
Preovulatory Follicles ◽  
Extensive Metabolism ◽  
Increased Sensitivity ◽  
Stimulation Of

The production of progesterone (P) and estrogen (E) by enzymatically dispersed granulosa and theca cells from chicken preovulatory follicles was examined in 3-h incubations. Accumulation of the P produced by granulosa cells was significantly reduced by the addition of theca cells, whereas E production was increased. The decrease in P accumulation was shown to be due to extensive metabolism of P by theca cells. There were no synergistic effects of luteinizing hormone (LH) and any substrate tested on E production by theca cells. Maturation of granulosa cells was characterized by an increased sensitivity to LH stimulation of P production, but there was no change in pregnenolone conversion to P. Conversely, maturation of theca cells was accompanied by decreased in both sensitivity to LH and the ability to convert substrates to E. The results are discussed in terms of the contribution of each cell type in the production of steroids by chicken follicles during maturation.

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