scholarly journals Pediococcus Acidilactici NRRL B-50517 as a Safe Probiotic for Regulation of Weight Related Pro-inflammatory Cytokines: A Double-blind Placebo-controlled Pilot Study (P21-026-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Jhy Jhu Lin ◽  
Jeanne O'Connell ◽  
Steve Sinclair
Keyword(s):  
Inflammatory Cytokines ◽  
Body Fat ◽  
Control Group ◽  
Human Consumption ◽  
P Value ◽  
Double Blind ◽  
Cytokine Analysis ◽  
Survival Studies ◽  
Mrs Broth ◽  
Pro Inflammatory Cytokines

Abstract Objectives Development of a probiotic P. acidilactici NRRL B-50517 that resists to high temperature (up to 85°C), stomach acids, and air. Clinic evaluation of P. acidilactici NRRL B-50517 as a safe probiotic for regulation of weight related pro-inflammatory cytokines. Methods Survival studies were conducted by incubating P. acidilactici NRRL B-50517 in either liquid MRS broth, pH1.5 at 85°C overnight, or in dried powder at 85°C for 5–10 minutes. Healthy volunters were randomly assigned two capsules of placebo or Pediococcus probiotics (4 billion CFU) daily for three months. Biweekly phone interviews with 30 participants were performed to ensure daily dosage administration, and to collect the results of the questionnaire related to 6 categories of gut functions. Percent body fat measurements and serum were collected upon study initiation and completion. Serum was processed for cytokine analysis by multiplex ELISA assays. Results P. acidilactici NRRL B-50,517 survived at 85°C and pH 1.5 in MRS broth and dried powder forms. No statistical significantly changes of gut functions between the placebo group and the probiotic group in appetite: t-value 1.6808, p-value 0.1313; energy level: t-value 0.7508. p-value 0.5003; bowel movement: t-value 0.3348. p-value 0.7464; stool quality: t-value 0.6929, p-value 0.5080; bloating: t-value 2.0583. p-value 0.0736; gas t-value: 1.9560, p-value 0.0875. On average, the probiotic lost 0.86% ± 0.42% body fat whereas the control group gained 0.28% ± 0.19, P = 0.0264. Pro-inflammaroty IL-6 ratios differed by 0.61 ± 0.22 and 3.06 ± 0.87 in the probiotic and control groups, respectively (P = 0.0295); pro-inflammatory IL23 ratio was 0.65 ± 0.14 in the probiotic and 1.71 ± 0.38 in the control group, P = 0.0068. Conclusions Novel P. acidilactici NRRL B-50,517 is safe for human consumption and may be useful in reducing body fat and pro-inflammatory markers. Funding Sources TEDCO, Maryland.

scholarly journals Effects of Onchung-eum, an Herbal Prescription, on 5-Fluorouracil–Induced Oral Mucositis

2018 ◽  
Vol 17 (4) ◽  
pp. 1285-1296
Author(s):  
Jae-Woo Park ◽  
Jayoung Oh ◽  
Seok-Jae Ko ◽  
Mun Seog Chang ◽  
Jinsung Kim
Keyword(s):  
Inflammatory Cytokines ◽  
Oral Mucositis ◽  
Caspase 3 ◽  
Skin Diseases ◽  
Nuclear Factor Κb ◽  
Control Group ◽  
Nuclear Factor ◽  
Cheek Pouches ◽  
Herbal Prescription ◽  
Pro Inflammatory Cytokines

In most cancer patients, chemotherapy-induced oral mucositis (OM) is a frequent side effect, leading to low quality of life and delay in therapy. The aim of this study was to evaluate the effects of Onchung-eum, a well-known herbal prescription in traditional medicine comprising 8 herbs that has long been used for skin diseases, on 5-fluorouracil (5-FU)–induced OM in human pharyngeal cells and golden Syrian hamsters. DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, and reactive oxygen species production were measured in vitro. The effects of Onchung-eum on OM of hamster cheek pouches induced by 5-FU were evaluated histologically and using TUNEL assay. In addition, the expression of nuclear factor-κB, caspase-3, and pro-inflammatory cytokines were measured by immunoblotting and immunohistochemistry. Significantly increased cell viability was observed in the Onchung-eum–treated groups compared with the 5-FU–treated control group. In 500 and 1000 mg/kg Onchung-eum–treated groups, the damaged epithelial layers in the cheek pouches of hamsters were significantly recovered. Moreover, at all concentrations, cell death in the cheek pouches of hamsters in the Onchung-eum–treated groups significantly decreased. The expression of pro-inflammatory cytokines, nuclear factor-κB, and caspase-3 also significantly decreased in Onchung-eum–treated groups at 500 and 1000 mg/kg. In conclusion, this study revealed that Onchung-eum can be used to treat chemotherapy-induced OM. However, further studies are required to understand the underlying mechanisms.

scholarly journals Analysis of the physical activity effects and measurement of pro-inflammatory cytokines in irradiated lungs in rats

2012 ◽  
Vol 27 (3) ◽  
pp. 223-230 ◽  
Author(s):  
Renata Cristiane Gennari Bianchi ◽  
Eduardo Rochete Ropelle ◽  
Carlos Kiyoshi Katashima ◽  
José Barreto Campello Carvalheira ◽  
Luiz Roberto Lopes ◽  
...  
Keyword(s):  
Physical Activity ◽  
Western Blotting ◽  
Inflammatory Cytokines ◽  
Lower Lobe ◽  
Whole Body ◽  
Control Group ◽  
Western Blotting Analysis ◽  
Blotting Analysis ◽  
Tnf Α ◽  
Pro Inflammatory Cytokines

PURPOSE: To study if the pre-radiotherapy physical activity has radio-protective elements, by measuring the radio-induced activation of pro-inflammatory cytokines as interleukin-6 (il-6), transforming growth factor -β (tgf -β), tumor necrosis factor -α (tnf-α) and protein beta kinase β (ikkβ), through western blotting analysis. METHODS: A randomized study with 28 Wistar hannover rats, males, with a mean age of 90 days and weighing about 200 grams. The animals were divided into three groups: (GI, GII and GIII). GIII group were submitted to swimming for eight weeks (zero load, three times a week, about 30 minutes). Then, the groups (except the control group) were submitted to irradiation by cobalt therapy, single dose of 3.5 gray in the whole body. All animals were sacrificed by overdose of pentobarbital, according to the time for analysis of cytokines, and then a fragment of the lower lobe of the right lung went to western blotting analysis. RESULTS: The cytokines IKK β, TNF-α and IL-6 induced by radiation in the lung were lower in the exercised animals. However, exercise did not alter the radiation-induced increase in tgf-β. CONCLUSION: The results show a lower response in relation to inflammatory cytokines in the group that practiced the exercise pre-radiotherapy, showing that exercise can protect tissues from tissue damage due to irradiation.

scholarly journals Associations between HBD3 and Pro-Inflammatory Cytokines in Asymptomatic Irreversible Pulpitis

2017 ◽  
pp. 14-19
Author(s):  
Anita Aminoshariae ◽  
Mohammed Bakkar ◽  
Tracey Bonfield ◽  
Santosh Ghosh ◽  
Thomas A Montagnese ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Normal Group ◽  
Control Group ◽  
Spearman Correlation ◽  
Whitney Test ◽  
Cytokines And Chemokines ◽  
Luminex Technology ◽  
Spearman Correlation Test ◽  
Irreversible Pulpitis ◽  
Pro Inflammatory Cytokines

Objective: The aim of this study was to investigate the levels of Human Beta Defensin (hBD) 2 and 3, chemokine and cytokine expressions between teeth endodontically diagnosed with symptomatic irreversible pulpitis (SIP), asymptomatic irreversible pulpitis (ASIP) and normal pulps. We hypothesized that there would be a correlation between hBD’s and the immunoregulatory response. Design: Pulpal samples were collected with paper points. Six samples were obtained from normal teeth, 21 from SIP, 18 from ASIP. Levels of cytokines and betadefensins were measured by Luminex technology and ELISA, respectively. Data were statistically analyzed using Kruskal-Wallis, Wilcoxon Mann-Whitney test and Spearman correlation test. Differences were considered significant at p<0.05. Results: hBD-2 levels correlated with samples obtained from patients in the ASIP group, but not in the samples obtained from patients with SIP or the control group. HBD-3 concentrations associated with all of the cytokines and chemokines in both SIP and ASIP groups. However, in the normal group, hBD-3 correlated with only TNFα, IL-8, MCP-1, IL-1β, MIP-1a, RANTES, IL-17 in normal group. When comparing control levels of hBD-2 and hBD-3 with patients samples from either the ASIP or the SIP groups, hBD-2 and hBD-3 concentrations were highest in the ASIP group. Conclusions: The hBD-2 and-3 were highly associated with the levels of the chemokines and cytokines in ASIP group. HBD-3 concentrations correlate with the levels of the chemokines and the cytokines in the SIP and ASIP groups.

scholarly journals Effect of Oral Ingestion of Low-Molecular Collagen Peptides Derived from Skate (Raja Kenojei) Skin on Body Fat in Overweight Adults: A Randomized, Double-Blind, Placebo-Controlled Trial

Marine Drugs ◽  
2019 ◽  
Vol 17 (3) ◽  
pp. 157 ◽  
Author(s):  
Young Tak ◽  
Yun Kim ◽  
Jeong Lee ◽  
Yu-Hyun Yi ◽  
Young Cho ◽  
...  
Keyword(s):  
Body Fat ◽  
Animal Studies ◽  
Controlled Trial ◽  
Intervention Group ◽  
Control Group ◽  
Double Blind ◽  
Collagen Peptides ◽  
Collagen Peptide ◽  
Skin Collagen ◽  
Overweight Adults

Recent animal studies found the potential of a collagen peptide derived from skate skin to have anti-obesity effects through the suppression of fat accumulation and regulation of lipid metabolism. However, no studies have yet been performed in humans. Here, this very first human randomized, placebo-controlled, and double-blinded study was designed to investigate the efficacy and tolerability of skate skin collagen peptides (SCP) for the reduction of body fat in overweight adults. Ninety healthy volunteers (17 men) aged 41.2 ± 10.4 years with a mean body mass index of 25.6 ± 1.9 kg/m2 were assigned to the intervention group (IG), which received 2000 mg of SCP per day or to the control group (CG) given the placebo for 12 weeks and 81 (90%) participants completed the study. Changes in body fat were evaluated using dual energy X-ray absorptiometry as a primary efficacy endpoint. After 12 weeks of the trial, the percentage of body fat and body fat mass (kg) in IG were found to be significantly better than those of subjects in CG (−1.2% vs. 2.7%, p = 0.024 and −1.2 kg vs. 0.3 kg, p = 0.025). Application of SCP was well tolerated and no notable adverse effect was reported from both groups. These results suggest the beneficial potential of SCP in the reduction of body fat in overweight adults.

scholarly journals Role of probiotics in lower reproductive tract infection in women of age group 18 to 45 years

2017 ◽  
Vol 6 (2) ◽  
pp. 671
Author(s):  
Rukshana Shamshu ◽  
Jayasree Vaman ◽  
Nirmala C.
Keyword(s):  
Bacterial Vaginosis ◽  
Lactobacillus Reuteri ◽  
Lactobacillus Rhamnosus ◽  
Vulvovaginal Candidiasis ◽  
Controlled Study ◽  
Control Group ◽  
P Value ◽  
Double Blind ◽  
Reproductive Tract Infection ◽  
Conventional Antibiotics

Background: This study has been designed to assess the effectiveness of the probiotic strains having Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 in the management of lower genital infections (bacterial vaginosis and candidiasis) as an integral therapy with antibiotics.Methods: Double blind, randomized placebo controlled study in women between 18-45 years attending Sree Avittom Thirunal Hospital (SATH), Government Medical College, Thiruvananthapuram, India with symptoms of bacterial vaginosis and vulvovaginal candidiasis. Women giving consent were given probiotics along with conventional antibiotics and were followed up over a period of two months. Outcome is measured as percentage of women showing a score of less than 4 at the end of thirty days of treatment, and sixty days of treatment in the test and control group in the case of bacterial vaginosis and no discharge and absence of hyphae and pseudohyphae in wet smear and gram stain in case of candidiasis.Results: Out of 42 women studied, 32 had bacterial vaginosis and 10 had vulvovaginal candidiasis. 81.3% women with BV had a normal vaginal picture after sixty days of treatment as compared to placebo which was only 31.3%. The p value is 0.004 which is significant. In the treatment of VC, the percentage of women cured was the same in both probiotic and placebo group.Conclusions: In the treatment of bacterial vaginosis, addition of probiotics to the conventional antibiotics can improve the cure rate of bacterial vaginosis and reduce the rate of recurrences. In the treatment of vulvovaginal candidiasis, addition of probiotics to the conventional antifungal did not have a significant improvement.

Microstructural changes and immunohistological analysis of pro-inflammatory cytokines in spleens of lipopolysaccharide-induced rats

2018 ◽  
Author(s):  
Y. B. Zhong ◽  
X. L. Zhang ◽  
M. Y. Lv ◽  
X. F. Hu ◽  
Y. Li
Keyword(s):  
Inflammatory Cytokines ◽  
Optical Density ◽  
Normal Saline ◽  
Interleukin 1 ◽  
Saline Group ◽  
Control Group ◽  
Sprague Dawley ◽  
Necrosis Factor Alpha ◽  
Lymphoid Follicles ◽  
Pro Inflammatory Cytokines

This study investigated splenic status changes in weaned Sprague-Dawley rats induced by lipopolysaccharide. There were forty 26-day-old rats selected randomly and equally divided into two groups. The treatment group received daily single doses of lipopolysaccharide, and the control group was treated with normal saline. We conducted haematoxylin-eosin staining, immunohistochemical staining and semi-quantitative optical density analysis for both groups on the 29th, 32nd, 35th and 38th days after treatment. The results indicated that splenic marginal zone in the lipopolysaccharide group was thinner or disappeared compared to that of the saline group. However, the periarterial lymphoid sheath and the diameters of splenic lymphoid follicles appeared thicker and wider than those in the saline group (P less than 0.05). The expression of interleukin-1 beta, interleukin-6 and tumour necrosis factor alpha was mainly localized within the periarterial lymphoid sheath and splenic lymphoid follicles in the lipopolysaccharide treated rats. The integrated optical density and the average optical density in the lipopolysaccharide group were greater than those in the normal saline treated group (P less than 0.05). In conclusion, splenic immune function is probably strengthened by altering microstructures and releasing pro-inflammatory cytokines following lipopolysaccharide treatment.

Changes of Vascular Endothelium Adhesive Properties in Patients with Chronic Generalized Slight Periodontitis During the Treatment

2015 ◽  
Vol 22 (4) ◽  
pp. 79-82
Author(s):  
Жданова ◽  
O. Zhdanova ◽  
Широков ◽  
V. Shirokov ◽  
Говорунова ◽  
...  
Keyword(s):  
Serum Concentration ◽  
Adhesion Molecules ◽  
Inflammatory Cytokines ◽  
Interleukin 1 ◽  
Intercellular Adhesion Molecule ◽  
Immunoglobulin Superfamily ◽  
Control Group ◽  
Adhesive Properties ◽  
Endothelial Adhesion Molecules ◽  
Pro Inflammatory Cytokines

The article presents the description of the changes in the concentrations of pro‐inflammatory cytokines and soluble forms of selectins and immunoglobulin superfamily adhesion molecules in patients with chronic generalized slight periodontitis. Serum concentrations of interleukin‐1‐β, tumor necrosis factor‐ α, soluble forms of P‐ and E‐selectins, intercellular adhesion molecule (ICAM‐1), vascular adhesion molecules (VCAM‐1) and platelet‐endothelial adhesion molecules (PECAM‐1) in healthy subjects and patients with slight periodontitis before and after treatment were assessed. It was found that the serum concentration of sICAM‐1, sVCAM‐1, sP‐, sE‐ selektins and pro‐inflammatory cytokines are increased in patients with chronic generalized periodontitis. The increase of serum concentration of soluble forms of selectins is expressed more significantly than sICAM‐1 and sVCAM‐1. There are no statistically significant changes of serum sPECAM‐1 in the examined group of patients in comparison with control. Complex therapy, including etiological and pathogenetic treatment, is completely normalized the concentration of pro‐inflammatory cytokines, and soluble forms of studied endothelial adhesion molecules in patients with chronic generalized slight periodontitis. All the studied parameters in patients with chronic generalized slight periodontitis after treatment are in the range of the control group variability.

scholarly journals Effects of Bupropion on Cognitive Function in Schizophrenia: A Double Blind Randomized Controlled Trial

2016 ◽  
Vol 9 (5) ◽  
pp. 67
Author(s):  
Mansoureh Mirzadeh ◽  
Najmeh Shahini ◽  
Masoud Kashani Lotf Abadi ◽  
Maryam Tavakoli ◽  
Arash Javanbakht ◽  
...  
Keyword(s):  
Randomized Controlled Trial ◽  
Cognitive Function ◽  
Rating Scale ◽  
Controlled Trial ◽  
Control Group ◽  
P Value ◽  
Double Blind ◽  
Randomized Controlled ◽  
Significant Difference ◽  
Schizophrenic Patients

<p>Smoking habits are common in schizophrenic patients. Nicotine can suppress negative symptoms and cognitive impairments. The aim of this study was to determine the efficacy of bupropion on cognitive function in schizophrenic patients.<strong> </strong>This study is a double blind randomized controlled trial in a large referral psychiatric university hospital in Iran. Ninety smoker schizophrenic patients were randomly allocated (based on DSM -IV TR criteria) in two groups (46 patients for case group and 44 patients in control group). They get risperidone up to 6 mg/d and bupropion up to 400 mg/d .clinical assessment (Positive and Negative Syndrome Scale (PANSS), Brief psychiatric rating scale (BPRS) were taken in beginning of study, 14<sup>th</sup> and 28<sup>th</sup> days of study. Cognitive assessment (Stroop, Digit Span, and Wechsler, Wisconsin) were taken in begging of study, the days 2<sup>nd</sup>, 7<sup>th</sup>, 14<sup>th</sup>, 28<sup>th</sup>. All data were analyzed by SPSS Ver. 17 with analytic and descriptive tests. Mean age of patients was 37.66±1.01. Mean duration of disorder was 11.63±.98 years. The scores were significantly lower at the day 28<sup>th</sup> compared to the beginning of the study in both groups in Wechsler, Stroop color word , Stroop word , Stroop color , BPRS, PANSS p value ≤0.05 .The difference between the two treatments was not significant as indicated by the effect of group, the between-subjects factor<strong> </strong><strong>p </strong>value ≥0.05. In this study, the side effects were examined and there was no significant difference between the two groups p value ≥0.05.<strong> </strong>Augmentation of bupropion to routine treatment improves cognitive symptoms of schizophrenia in abstinence of tobacco.</p>

scholarly journals CHANGES IN IMMUNITY PARAMETERS DURING RADIОTHERAPY FOR HEAD AND NECK CANCER DEPENDING ON THE METHOD OF RADIATION TOXICITY PREVENTION

2021 ◽  
Vol 19 (2) ◽  
pp. 212-218
Author(s):  
L. B. Parkhomenko ◽  
Keyword(s):  
Head And Neck Cancer ◽  
Acute Toxicity ◽  
Head And Neck ◽  
Inflammatory Cytokines ◽  
Neck Cancer ◽  
Randomized Study ◽  
Comparative Assessment ◽  
Control Group ◽  
Radiation Toxicity ◽  
Pro Inflammatory Cytokines

Background: The use of magnetolaser and local hypoxia during radiotherapy for head and neck cancer leads to decrease in acute toxicity. Purpose: To reduce the severity of acute toxicity in patients with head and neck cancer using the developed methods of prevention. Material and Methods: A prospective randomized study included 120 patients with head and neck cancer. A comparative assessment of the effectiveness of the three developed methods of prevention was carried out in comparison with traditional prevention. Results: The use of magnetolaser, local hypoxia and their combination led to decrease in the level of pro-inflammatory cytokines and increase in anti-inflammatory cytokines as compared with the control group. This corresponded to a lesser degree of acute toxicity in the main groups. Conclusions: The use of magnetolaser and/or hypoxia reduces acute toxicity, which is confirmed by changes in immunity parameters.

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