scholarly journals Associations between HBD3 and Pro-Inflammatory Cytokines in Asymptomatic Irreversible Pulpitis

2017 ◽  
pp. 14-19
Author(s):  
Anita Aminoshariae ◽  
Mohammed Bakkar ◽  
Tracey Bonfield ◽  
Santosh Ghosh ◽  
Thomas A Montagnese ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Normal Group ◽  
Control Group ◽  
Spearman Correlation ◽  
Whitney Test ◽  
Cytokines And Chemokines ◽  
Luminex Technology ◽  
Spearman Correlation Test ◽  
Irreversible Pulpitis ◽  
Pro Inflammatory Cytokines

Objective: The aim of this study was to investigate the levels of Human Beta Defensin (hBD) 2 and 3, chemokine and cytokine expressions between teeth endodontically diagnosed with symptomatic irreversible pulpitis (SIP), asymptomatic irreversible pulpitis (ASIP) and normal pulps. We hypothesized that there would be a correlation between hBD’s and the immunoregulatory response. Design: Pulpal samples were collected with paper points. Six samples were obtained from normal teeth, 21 from SIP, 18 from ASIP. Levels of cytokines and betadefensins were measured by Luminex technology and ELISA, respectively. Data were statistically analyzed using Kruskal-Wallis, Wilcoxon Mann-Whitney test and Spearman correlation test. Differences were considered significant at p<0.05. Results: hBD-2 levels correlated with samples obtained from patients in the ASIP group, but not in the samples obtained from patients with SIP or the control group. HBD-3 concentrations associated with all of the cytokines and chemokines in both SIP and ASIP groups. However, in the normal group, hBD-3 correlated with only TNFα, IL-8, MCP-1, IL-1β, MIP-1a, RANTES, IL-17 in normal group. When comparing control levels of hBD-2 and hBD-3 with patients samples from either the ASIP or the SIP groups, hBD-2 and hBD-3 concentrations were highest in the ASIP group. Conclusions: The hBD-2 and-3 were highly associated with the levels of the chemokines and cytokines in ASIP group. HBD-3 concentrations correlate with the levels of the chemokines and the cytokines in the SIP and ASIP groups.

scholarly journals Effects of Onchung-eum, an Herbal Prescription, on 5-Fluorouracil–Induced Oral Mucositis

2018 ◽  
Vol 17 (4) ◽  
pp. 1285-1296
Author(s):  
Jae-Woo Park ◽  
Jayoung Oh ◽  
Seok-Jae Ko ◽  
Mun Seog Chang ◽  
Jinsung Kim
Keyword(s):  
Inflammatory Cytokines ◽  
Oral Mucositis ◽  
Caspase 3 ◽  
Skin Diseases ◽  
Nuclear Factor Κb ◽  
Control Group ◽  
Nuclear Factor ◽  
Cheek Pouches ◽  
Herbal Prescription ◽  
Pro Inflammatory Cytokines

In most cancer patients, chemotherapy-induced oral mucositis (OM) is a frequent side effect, leading to low quality of life and delay in therapy. The aim of this study was to evaluate the effects of Onchung-eum, a well-known herbal prescription in traditional medicine comprising 8 herbs that has long been used for skin diseases, on 5-fluorouracil (5-FU)–induced OM in human pharyngeal cells and golden Syrian hamsters. DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, and reactive oxygen species production were measured in vitro. The effects of Onchung-eum on OM of hamster cheek pouches induced by 5-FU were evaluated histologically and using TUNEL assay. In addition, the expression of nuclear factor-κB, caspase-3, and pro-inflammatory cytokines were measured by immunoblotting and immunohistochemistry. Significantly increased cell viability was observed in the Onchung-eum–treated groups compared with the 5-FU–treated control group. In 500 and 1000 mg/kg Onchung-eum–treated groups, the damaged epithelial layers in the cheek pouches of hamsters were significantly recovered. Moreover, at all concentrations, cell death in the cheek pouches of hamsters in the Onchung-eum–treated groups significantly decreased. The expression of pro-inflammatory cytokines, nuclear factor-κB, and caspase-3 also significantly decreased in Onchung-eum–treated groups at 500 and 1000 mg/kg. In conclusion, this study revealed that Onchung-eum can be used to treat chemotherapy-induced OM. However, further studies are required to understand the underlying mechanisms.

scholarly journals Analysis of the physical activity effects and measurement of pro-inflammatory cytokines in irradiated lungs in rats

2012 ◽  
Vol 27 (3) ◽  
pp. 223-230 ◽  
Author(s):  
Renata Cristiane Gennari Bianchi ◽  
Eduardo Rochete Ropelle ◽  
Carlos Kiyoshi Katashima ◽  
José Barreto Campello Carvalheira ◽  
Luiz Roberto Lopes ◽  
...  
Keyword(s):  
Physical Activity ◽  
Western Blotting ◽  
Inflammatory Cytokines ◽  
Lower Lobe ◽  
Whole Body ◽  
Control Group ◽  
Western Blotting Analysis ◽  
Blotting Analysis ◽  
Tnf Α ◽  
Pro Inflammatory Cytokines

PURPOSE: To study if the pre-radiotherapy physical activity has radio-protective elements, by measuring the radio-induced activation of pro-inflammatory cytokines as interleukin-6 (il-6), transforming growth factor -β (tgf -β), tumor necrosis factor -α (tnf-α) and protein beta kinase β (ikkβ), through western blotting analysis. METHODS: A randomized study with 28 Wistar hannover rats, males, with a mean age of 90 days and weighing about 200 grams. The animals were divided into three groups: (GI, GII and GIII). GIII group were submitted to swimming for eight weeks (zero load, three times a week, about 30 minutes). Then, the groups (except the control group) were submitted to irradiation by cobalt therapy, single dose of 3.5 gray in the whole body. All animals were sacrificed by overdose of pentobarbital, according to the time for analysis of cytokines, and then a fragment of the lower lobe of the right lung went to western blotting analysis. RESULTS: The cytokines IKK β, TNF-α and IL-6 induced by radiation in the lung were lower in the exercised animals. However, exercise did not alter the radiation-induced increase in tgf-β. CONCLUSION: The results show a lower response in relation to inflammatory cytokines in the group that practiced the exercise pre-radiotherapy, showing that exercise can protect tissues from tissue damage due to irradiation.

scholarly journals Pediococcus Acidilactici NRRL B-50517 as a Safe Probiotic for Regulation of Weight Related Pro-inflammatory Cytokines: A Double-blind Placebo-controlled Pilot Study (P21-026-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Jhy Jhu Lin ◽  
Jeanne O'Connell ◽  
Steve Sinclair
Keyword(s):  
Inflammatory Cytokines ◽  
Body Fat ◽  
Control Group ◽  
Human Consumption ◽  
P Value ◽  
Double Blind ◽  
Cytokine Analysis ◽  
Survival Studies ◽  
Mrs Broth ◽  
Pro Inflammatory Cytokines

Abstract Objectives Development of a probiotic P. acidilactici NRRL B-50517 that resists to high temperature (up to 85°C), stomach acids, and air. Clinic evaluation of P. acidilactici NRRL B-50517 as a safe probiotic for regulation of weight related pro-inflammatory cytokines. Methods Survival studies were conducted by incubating P. acidilactici NRRL B-50517 in either liquid MRS broth, pH1.5 at 85°C overnight, or in dried powder at 85°C for 5–10 minutes. Healthy volunters were randomly assigned two capsules of placebo or Pediococcus probiotics (4 billion CFU) daily for three months. Biweekly phone interviews with 30 participants were performed to ensure daily dosage administration, and to collect the results of the questionnaire related to 6 categories of gut functions. Percent body fat measurements and serum were collected upon study initiation and completion. Serum was processed for cytokine analysis by multiplex ELISA assays. Results P. acidilactici NRRL B-50,517 survived at 85°C and pH 1.5 in MRS broth and dried powder forms. No statistical significantly changes of gut functions between the placebo group and the probiotic group in appetite: t-value 1.6808, p-value 0.1313; energy level: t-value 0.7508. p-value 0.5003; bowel movement: t-value 0.3348. p-value 0.7464; stool quality: t-value 0.6929, p-value 0.5080; bloating: t-value 2.0583. p-value 0.0736; gas t-value: 1.9560, p-value 0.0875. On average, the probiotic lost 0.86% ± 0.42% body fat whereas the control group gained 0.28% ± 0.19, P = 0.0264. Pro-inflammaroty IL-6 ratios differed by 0.61 ± 0.22 and 3.06 ± 0.87 in the probiotic and control groups, respectively (P = 0.0295); pro-inflammatory IL23 ratio was 0.65 ± 0.14 in the probiotic and 1.71 ± 0.38 in the control group, P = 0.0068. Conclusions Novel P. acidilactici NRRL B-50,517 is safe for human consumption and may be useful in reducing body fat and pro-inflammatory markers. Funding Sources TEDCO, Maryland.

Microstructural changes and immunohistological analysis of pro-inflammatory cytokines in spleens of lipopolysaccharide-induced rats

2018 ◽  
Author(s):  
Y. B. Zhong ◽  
X. L. Zhang ◽  
M. Y. Lv ◽  
X. F. Hu ◽  
Y. Li
Keyword(s):  
Inflammatory Cytokines ◽  
Optical Density ◽  
Normal Saline ◽  
Interleukin 1 ◽  
Saline Group ◽  
Control Group ◽  
Sprague Dawley ◽  
Necrosis Factor Alpha ◽  
Lymphoid Follicles ◽  
Pro Inflammatory Cytokines

This study investigated splenic status changes in weaned Sprague-Dawley rats induced by lipopolysaccharide. There were forty 26-day-old rats selected randomly and equally divided into two groups. The treatment group received daily single doses of lipopolysaccharide, and the control group was treated with normal saline. We conducted haematoxylin-eosin staining, immunohistochemical staining and semi-quantitative optical density analysis for both groups on the 29th, 32nd, 35th and 38th days after treatment. The results indicated that splenic marginal zone in the lipopolysaccharide group was thinner or disappeared compared to that of the saline group. However, the periarterial lymphoid sheath and the diameters of splenic lymphoid follicles appeared thicker and wider than those in the saline group (P less than 0.05). The expression of interleukin-1 beta, interleukin-6 and tumour necrosis factor alpha was mainly localized within the periarterial lymphoid sheath and splenic lymphoid follicles in the lipopolysaccharide treated rats. The integrated optical density and the average optical density in the lipopolysaccharide group were greater than those in the normal saline treated group (P less than 0.05). In conclusion, splenic immune function is probably strengthened by altering microstructures and releasing pro-inflammatory cytokines following lipopolysaccharide treatment.

Changes of Vascular Endothelium Adhesive Properties in Patients with Chronic Generalized Slight Periodontitis During the Treatment

2015 ◽  
Vol 22 (4) ◽  
pp. 79-82
Author(s):  
Жданова ◽  
O. Zhdanova ◽  
Широков ◽  
V. Shirokov ◽  
Говорунова ◽  
...  
Keyword(s):  
Serum Concentration ◽  
Adhesion Molecules ◽  
Inflammatory Cytokines ◽  
Interleukin 1 ◽  
Intercellular Adhesion Molecule ◽  
Immunoglobulin Superfamily ◽  
Control Group ◽  
Adhesive Properties ◽  
Endothelial Adhesion Molecules ◽  
Pro Inflammatory Cytokines

The article presents the description of the changes in the concentrations of pro‐inflammatory cytokines and soluble forms of selectins and immunoglobulin superfamily adhesion molecules in patients with chronic generalized slight periodontitis. Serum concentrations of interleukin‐1‐β, tumor necrosis factor‐ α, soluble forms of P‐ and E‐selectins, intercellular adhesion molecule (ICAM‐1), vascular adhesion molecules (VCAM‐1) and platelet‐endothelial adhesion molecules (PECAM‐1) in healthy subjects and patients with slight periodontitis before and after treatment were assessed. It was found that the serum concentration of sICAM‐1, sVCAM‐1, sP‐, sE‐ selektins and pro‐inflammatory cytokines are increased in patients with chronic generalized periodontitis. The increase of serum concentration of soluble forms of selectins is expressed more significantly than sICAM‐1 and sVCAM‐1. There are no statistically significant changes of serum sPECAM‐1 in the examined group of patients in comparison with control. Complex therapy, including etiological and pathogenetic treatment, is completely normalized the concentration of pro‐inflammatory cytokines, and soluble forms of studied endothelial adhesion molecules in patients with chronic generalized slight periodontitis. All the studied parameters in patients with chronic generalized slight periodontitis after treatment are in the range of the control group variability.

scholarly journals Bromelain treatment decreases secretion of pro-inflammatory cytokines and chemokines by colon biopsies in vitro

2008 ◽  
Vol 126 (3) ◽  
pp. 345-352 ◽  
Author(s):  
Jane E. Onken ◽  
Paula K. Greer ◽  
Brian Calingaert ◽  
Laura P. Hale
Keyword(s):  
Inflammatory Cytokines ◽  
Cytokines And Chemokines ◽  
Pro Inflammatory Cytokines

scholarly journals CHANGES IN IMMUNITY PARAMETERS DURING RADIОTHERAPY FOR HEAD AND NECK CANCER DEPENDING ON THE METHOD OF RADIATION TOXICITY PREVENTION

2021 ◽  
Vol 19 (2) ◽  
pp. 212-218
Author(s):  
L. B. Parkhomenko ◽  
Keyword(s):  
Head And Neck Cancer ◽  
Acute Toxicity ◽  
Head And Neck ◽  
Inflammatory Cytokines ◽  
Neck Cancer ◽  
Randomized Study ◽  
Comparative Assessment ◽  
Control Group ◽  
Radiation Toxicity ◽  
Pro Inflammatory Cytokines

Background: The use of magnetolaser and local hypoxia during radiotherapy for head and neck cancer leads to decrease in acute toxicity. Purpose: To reduce the severity of acute toxicity in patients with head and neck cancer using the developed methods of prevention. Material and Methods: A prospective randomized study included 120 patients with head and neck cancer. A comparative assessment of the effectiveness of the three developed methods of prevention was carried out in comparison with traditional prevention. Results: The use of magnetolaser, local hypoxia and their combination led to decrease in the level of pro-inflammatory cytokines and increase in anti-inflammatory cytokines as compared with the control group. This corresponded to a lesser degree of acute toxicity in the main groups. Conclusions: The use of magnetolaser and/or hypoxia reduces acute toxicity, which is confirmed by changes in immunity parameters.

scholarly journals Influence of hyperthyroidism on hepatic antioxidants and cytokines Levels: An Experimental Study

2020 ◽  
Vol 7 (3) ◽  
pp. 439-444
Author(s):  
Nurten Bahtiyar ◽  
Aysun Yoldaş ◽  
Birsen Aydemir ◽  
Selmin Toplan
Keyword(s):  
Inflammatory Cytokines ◽  
Inductively Coupled Plasma ◽  
Optical Emission ◽  
Control Group ◽  
Inductively Coupled ◽  
Inflammatory Status ◽  
Tnf Α ◽  
Tissue Homogenates ◽  
Pro Inflammatory Cytokines ◽  
Liver Tissues

Objective: Thyroid diseases greatly affect the liver. Hyperthyroidism can affect the function of the liver. This study aimed to investigate the possible change of antioxidant and pro-inflammatory cytokines levels in liver tissue in hyperthyroid rats. Material and Methods: This study was carried out with 2 experimental groups. Hyperthyroid group was fed with 4 mg/kg L-thyroxine added standard fodder. Control group was fed with standard rat fodder. Liver selenium (Se) levels were measured by inductively coupled plasma optical emission spectrophotometer (ICP-OES). The antioxidant markers such as Selenoprotein P (SelP), and glutathione peroxidase (GPx), and the pro-inflammatory cytokines such as Interleukin (IL)-18, and Tumor necrosis factor-α (TNF-α) levels were studied in liver tissues by ELISA. All markers levels of liver samples were measured in tissue homogenates. Results: Se, SelP, and GPx levels of the hyperthyroidism group were lower than the control group. (p=0.038, p=0.046, p=0.008 respectively). There was a significant increase in IL-18 and TNF-α levels in hyperthyroidism group when compared to control group (p=0.002, p=0.023 respectively). There was positive correlation between FT3 and FT4, IL-18 and TNF-α (r=0.761, r=0.843, and r=0.826 respectively), but there was negative correlation between FT3 and Se, SelP, and GPx (r=-0.833, r=-0.754, and r=-0.778 respectively). Conclusion: Our findings showed that antioxidant marker levels were decreased, and pro-inflammatory cytokine levels were increased in liver tissues of hyperthyroid rats. These findings suggest that impaired antioxidant and pro-inflammatory status may play a role in liver pathogenesis due to hyperthyroidism.

scholarly journals Combating COVID-19 with Mesenchymal Stem Cell therapy

2020 ◽  
Author(s):  
Shashikant Ray ◽  
Keshav Rajarshi ◽  
Aroni Chatterjee
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Therapy ◽  
Inflammatory Cytokines ◽  
Stem Cell Therapy ◽  
Rna Virus ◽  
Multiple Organ ◽  
Mesenchymal Stem Cell Therapy ◽  
Cytokines And Chemokines ◽  
Pro Inflammatory Cytokines

The world is currently facing one of its deadliest nightmares, the rise of a global pandemic called COVID-19. The disease is caused by a positive stranded RNA virus called SARS-CoV-2. The virus mainly targets the pulmonary epithelial cells as it’s initial site of infection by letting its surface spike protein interact and bind to the host ACE2 receptor. The internalization and gradual replication of the virus results in an exaggerated immune response triggering release of many pro-inflammatory cytokines and chemokines. This immune storm is responsible for multiple health hazards in the host ultimately leading to multiple organ failure. Mesenchymal stem cell therapy offers a promising approach towards mitigating the delirious effects of the infection in the COVID-19 patients. This therapy has shown to reduce the expression of pro-inflammatory cytokines as well as repair of damaged tissues in COVID-19 patients. This review has been organized to put forward all the positive aruments and implications in support of mesenchymal stem cell therapy as a necessary approach for treating COVID-19 patients.

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