Silicic Acid Chromatography of Organic Acids in Blood Cells and Biological Fluids

1970 ◽  
Vol 16 (1) ◽  
pp. 20-23 ◽  
Author(s):  
Lewis A Barness ◽  
Grant Morrow ◽  
Robert E Nocho ◽  
Rita A Maresca
Keyword(s):  
Organic Acids ◽  
Silicic Acid ◽  
Blood Cells ◽  
Quantitative Measurement ◽  
Color Change ◽  
Biological Fluids ◽  
Spinal Fluid ◽  
Silicic Acid Chromatography ◽  
Color Yield ◽  
Peak Areas

Abstract The separation of organic acids and their elution from silicic acid columns and their quantitative measurement by color change of buffered indicator, a technique mechanized by Kesner and Muntwyler, are applicable to the study of biological materials. As little as 25 µg of acid can be measured. Elution times and color yield in terms of peak areas obtained with the commercial apparatus are given for 25 organic acids. Blood, plasma, red cells, and spinal fluid have been analyzed; specimens were either untreated or extracted with ether. The most abundant acids detected were propionic, lactic, hippuric, and acetic. Methylmalonate was measured in body fluids and blood cells of patients with methylmalonic acidemia and pernicious anemia. Concentrations of organic acids in blood and spinal fluid are given.

Effect of Phenobarbitone Treatment on Vitamin D Metabolism in Mammals

1974 ◽  
Vol 46 (4) ◽  
pp. 433-448 ◽  
Author(s):  
J. Silver ◽  
G. Neale ◽  
G. R. Thompson
Keyword(s):  
Vitamin D ◽  
Biological Activity ◽  
Silicic Acid ◽  
Vitamin D3 ◽  
Water Soluble ◽  
Prolonged Treatment ◽  
Vitamin D Metabolism ◽  
Silicic Acid Chromatography ◽  
Polar Metabolites ◽  
25 Hydroxycholecalciferol

1. The metabolism of radioactive cholecalciferol was studied in control and phenobarbitone-treated rats and pigs. 2. Treatment with phenobarbitone enhanced the appearance in plasma of 25-hydroxycholecalciferol (peak IV on silicic acid chromatography), and of more-polar metabolites (peak V), but not of the most-polar metabolites (peak VI). Peak IV had the chromatographic properties of authentic 25-hydroxycholecalciferol (25-HCC) and had biological activity. 3. There was no effect on the appearance of peaks V and VI in plasma after an injection of radioactive 25-HCC. 4. Treatment with phenobarbitone enhanced the excretion of metabolites of radioactive vitamin D3 in bile. These metabolites were largely water-soluble conjugates of peaks IV, V and VI, which included glucuronides. Peak IV in bile was not identical with 25-HCC. 5. Prolonged treatment with phenobarbitone depleted the tissue radioactivity of rats given radioactive vitamin D3.

Lipid changes in cardiac hypertrophy as measured by silicic acid chromatography

1963 ◽  
Vol 204 (2) ◽  
pp. 297-300 ◽  
Author(s):  
G. H. Nelson ◽  
D. M. Bear ◽  
T. O. Dotson ◽  
R. F. Krause
Keyword(s):  
Cardiac Hypertrophy ◽  
Silicic Acid ◽  
Total Lipid ◽  
Atmospheric Pressure ◽  
Silicic Acid Column ◽  
Prolonged Exposure ◽  
Phospholipid Content ◽  
Albino Rats ◽  
Silicic Acid Column Chromatography ◽  
Silicic Acid Chromatography

Cardiac hypertrophy was produced in adult, male albino rats by prolonged exposure to reduced atmospheric pressure. Total lipid extracts of the heart were made and the lipids were separated into seven fractions by silicic acid column chromatography. The purity of each fraction was determined by chemical analysis. Fractions II, IV, and VII were found to contain reasonably pure triglyceride, cholesterol, and phospholipid, respectively. Fractions I, III, V, and VI contained cholesterol ester, free fatty acid, diglyceride, and monoglyceride, respectively, but were contaminated with other lipid material. The hypertrophied hearts showed a reduction in percentage of total lipid but no change in the weight of total lipid per heart. As the heart increased in weight the phospholipid content increased and the weight of cholesterol decreased. These findings confirm previous observations that the phospholipid content of muscles increases with activity.

scholarly journals Quantitative Measurement of Organic Acids in Tissues from Gastric Cancer Patients Indicates Increased Glucose Metabolism in Gastric Cancer

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e98581 ◽  
Author(s):  
Hoon Hur ◽  
Man Jeong Paik ◽  
Yi Xuan ◽  
Duc-Toan Nguyen ◽  
In-Hye Ham ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Glucose Metabolism ◽  
Organic Acids ◽  
Cancer Patients ◽  
Quantitative Measurement ◽  
Gastric Cancer Patients

scholarly journals Distribution of prostaglandins in rabbit kidney

1970 ◽  
Vol 116 (3) ◽  
pp. 421-424 ◽  
Author(s):  
Keith Crowshaw ◽  
J. Z. Szlyk
Keyword(s):  
Fatty Acids ◽  
Arachidonic Acid ◽  
Sodium Hydroxide ◽  
Silicic Acid ◽  
Rabbit Kidney ◽  
Kidney Cortex ◽  
Kidney Medulla ◽  
Silicic Acid Chromatography ◽  
Before And After ◽  
Weak Activity

Three prostaglandins (PGE2, PGF2α and PGA2) are present in rabbit kidney medulla. An acidic lipid extract (0.165g) obtained from 2kg of frozen rabbit kidney cortex was separated by silicic acid chromatography to yield eluates containing fatty acids, possible non-polar prostaglandin metabolites, PGA, PGE and PGF compounds. Ultraviolet spectra of the eluates before and after treatment with sodium hydroxide did not yield chromophores typical of any known prostaglandins or related metabolites. By using more sensitive bioassay procedures (contraction of rabbit duodenum) weak activity equivalent to 60μg of PGE2 and 10μg of PGF2α was detected in the PGE and PGF eluates respectively. Extraction and bioassay of fresh kidney cortex revealed no prostaglandin-like activity. Attempts to biosynthesize prostaglandins in fresh homogenates of rabbit kidney cortex from endogenous precursors and from added arachidonic acid were unsuccessful. When freshly prepared homogenates of rabbit kidney cortex were incubated with added PGE1 no evidence of enzymic breakdown was obtained. It is concluded that rabbit kidney prostaglandins are present predominantly in the medulla and there are no cortical mechanisms for their biosynthesis or inactivation under normal conditions.

scholarly journals Kemampuan Reagen Curcumax Mendeteksi Boraks dalam Bakso yang Direbus

2017 ◽  
Vol 35 (1) ◽  
pp. 42
Author(s):  
Erna Dwi Astuti ◽  
Widagdo Sri Nugroho
Keyword(s):  
Treatment Group ◽  
Human Health ◽  
Quantitative Measurement ◽  
Color Change ◽  
Wave Length ◽  
Treatment Unit ◽  
Significant Difference

Borax is a harmful compound to human health but sometimes was used illegally as preservative in meatball. Curcumax reagent is a compound which developed from the previous borax detector base on tumeric extract. This reagen was developed as an easy kit to apply in the field. This study aims were to determine accuracy of sight obesrvation and spectrophotometer to detected borax in boiled meatballs which tested by Curcumax and to determine the effect of boiling on the borax presence in the meatballs. This study was using meatballs groupas a control and another meatballs group preserve with borax 0.5%. Each treatment group was boiled in 0, 5, 10, and 15 minutes and each treatment unit had three replications which consist five meatballs each of them. Boraxdetection was done by mixing 1ml Curcumax reagent into 1 ml meatball extract. Qualitative examination of borax presence in the meatballs was known based on the color changing of tested meatball’s extract from yellow became orange (direct sight with eyes). The Quantitaive analysis was done by measured using spectrophotometer base on wave-length (λ) at 570 nm. Data was analyzed with ANOVA and continued by Least Significant Difference (LSD) analysis. Borax detection using curcumax based on sight sense against color change giving an inconsistence result. Meanwhile quantitative measurement by spectrophotometer more accurate than one. Curcumax had been detected borax in meatball which boiled for 15 minutes.

scholarly journals Quantitative measurement of axial accumulation of red blood cells in micro channel using micro PTV

2007 ◽  
Vol 27 (Supplement1) ◽  
pp. 139-140
Author(s):  
Hitoshi Yabusaki ◽  
Alexandros Charalambides ◽  
Masamichi Oishi ◽  
Haruyuki Kinoshita ◽  
Marie Oshima
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Quantitative Measurement ◽  
Micro Channel
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