scholarly journals SCNBase: a genomics portal for the soybean cyst nematode (Heterodera glycines)

Database ◽  
2019 ◽  
Vol 2019 ◽  
Author(s):  
Rick Masonbrink ◽  
Tom R Maier ◽  
Arun S Seetharam ◽  
Parijat S Juvale ◽  
Levi Baber ◽  
...  
Keyword(s):  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
Orthologous Gene ◽  
Nematode Species ◽  
Cyst Nematode ◽  
Future Research ◽  
Parasitic Nematodes ◽  
Management Tools ◽  
Genomic Resources ◽  
Gene Annotations

AbstractSoybean is an important worldwide crop, and farmers continue to experience significant yield loss due to the soybean cyst nematode (SCN), Heterodera glycines. This soil-borne roundworm parasite is rated the most important pathogen problem in soybean production. The infective nematodes enter into complex interactions with their host plant by inducing the development of specialized plant feeding cells that provide the parasites with nourishment. Addressing the SCN problem will require the development of genomic resources and a global collaboration of scientists to analyze and use these resources. SCNBase.org was designed as a collaborative hub for the SCN genome. All data and analyses are downloadable and can be analyzed with three integrated genomic tools: JBrowse, Feature Search and BLAST. At the time of this writing, a number of genomic and transcriptomic data sets are already available, with 43 JBrowse tracks and 21 category pages describing SCN genomic analyses on gene predictions, transcriptome and read alignments, effector-like genes, expansion and contraction of genomic repeats, orthology and synteny with related nematode species, Single Nucleotide Polymorphism (SNPs) from 15 SCN populations and novel splice sites. Standard functional gene annotations were supplemented with orthologous gene annotations using a comparison to nine related plant-parasitic nematodes, thereby enabling functional annotations for 85% of genes. These annotations led to a greater grasp on the SCN effectorome, which include over 3324 putative effector genes. By designing SCNBase as a hub, future research findings and genomic resources can easily be uploaded and made available for use by others with minimal needs for further curation. By providing these resources to nematode research community, scientists will be empowered to develop novel, more effective SCN management tools.

The beet cyst nematode (Heterodera schachtii): an ancient threat to sugar beet crops in Central Europe has become an invisible actor.

2021 ◽  
pp. 394-399
Author(s):  
Matthias Daub
Keyword(s):  
Sugar Beet ◽  
Integrated Management ◽  
Nematode Species ◽  
Cyst Nematode ◽  
Heterodera Schachtii ◽  
Future Research ◽  
Parasitic Nematodes ◽  
Beet Cyst Nematode ◽  
Management Optimization ◽  
Damage Symptoms

Abstract The beet cyst nematode (BCN) was one of the first discovered plant parasitic nematodes. Heterodera schachtii was observed in 1859 in Halle in Central Germany by the botanist Herman Schacht and described later by Adolf Schmidt in 1871, who named this cyst nematode species after its original discoverer. Partly due to the lack of knowledge about the effect of sugar beet monocultures on the population build-up of BCN, this nematode had a devastating impact on sugar production in 1876 that led to the shutdown of 24 sugar factories in Germany. This chapter discusses the economic importance, distribution, host range, damage symptoms, biology and life cycle, interactions with other nematodes and pathogens, recommended integrated management, and management optimization of H. schachtii. Future research requirements and future developments are also mentioned.

Transgenic soybeans expressing siRNAs specific to a major sperm protein gene suppress Heterodera glycines reproduction

2006 ◽  
Vol 33 (11) ◽  
pp. 991 ◽  
Author(s):  
Ryan M. Steeves ◽  
Tim C. Todd ◽  
Juliane S. Essig ◽  
Harold N. Trick
Keyword(s):  
Soybean Cyst Nematode ◽  
Yield Loss ◽  
Heterodera Glycines ◽  
Control Strategies ◽  
Reproductive Potential ◽  
Cyst Nematode ◽  
Root Tissue ◽  
Parasitic Nematodes ◽  
Major Sperm Protein ◽  
Sperm Protein

The soybean cyst nematode (SCN), Heterodera glycines, is the major disease-causing agent limiting soybean production in the USA. The current management strategy to reduce yield loss by SCN involves the deployment of resistant soybean cultivars and rotation to non-host crops. Although this management scheme has shown some success, continued yearly yield loss estimates demonstrate the limitations of these techniques. As a result, new control strategies are needed to complement the existing methods. Reported here is a novel method of SCN control that utilises RNA interference (RNAi). Transgenic soybeans were generated following transformation with an RNAi expression vector containing inverted repeats of a cDNA clone of the major sperm protein (MSP) gene from H. glycines. The accumulation of MSP-specific short interfering RNA (siRNA) molecules were detected by northern blot analysis of transgenic soybeans. T0 plants displaying MSP siRNA accumulation were deployed in a bioassay to evaluate the effects of MSP interfering molecules on H. glycines reproduction. Bioassay data has shown up to a 68% reduction in eggs g–1 root tissue, demonstrating that MSPi transgenic plants significantly reduced the reproductive potential of H. glycines. An additional bioassay evaluating progeny nematodes for maintenance of reproductive suppression indicated that progeny were also impaired in their ability to successfully reproduce, as demonstrated by a 75% reduction in eggs g–1 root tissue. The results of this study demonstrate the efficacy of an RNAi-based strategy for control of the soybean cyst nematode. In addition, these results may have important implications for the control of other plant parasitic nematodes.

Occurrence of the White Soybean Cyst Nematode, Heterodera sojae, and the Soybean Cyst Nematode, H. glycines, in Korea

Plant Disease ◽  
2021 ◽  
Vol 105 (1) ◽  
pp. 31-33 ◽  
Author(s):  
Heonil Kang ◽  
Hyoungrai Ko ◽  
Donggeun Kim ◽  
Insoo Choi
Keyword(s):  
Glycine Max ◽  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
Nematode Species ◽  
Wide Distribution ◽  
Cyst Nematode ◽  
Soil Samples ◽  
Cyst Nematodes ◽  
Glycine Max L ◽  
Soybean Plants

Soybean cyst nematode (Heterodera glycines, SCN) is the most harmful pathogen of soybean (Glycine max (L.) Merr.) worldwide. In 2016, a new soybean-parasitic cyst nematode, Heterodera sojae (the white soybean cyst nematode) was found parasitizing the roots of soybean plants in Korea. To investigate the distribution and population density of H. sojae, 943 soil samples were collected from soybean fields in all nine provinces in Korea in 2017 to 2018. Cyst nematodes were detected in 343 samples (36.4%) from eight of the nine provinces, except the island of Jeju province. Among the 343 samples, H. glycines was found in 227 samples (66.2%), H. sojae in 95 samples (27.7%), and 21 samples (6.1%) were infested with both H. sojae and H. glycines. Wide distribution of H. sojae in soybean fields indicates that H. sojae is an important cyst nematode species parasitizing soybean together with H. glycines.

scholarly journals Discovery and initial analysis of novel viral genomes in the soybean cyst nematode

2011 ◽  
Vol 92 (8) ◽  
pp. 1870-1879 ◽  
Author(s):  
Sadia Bekal ◽  
Leslie L. Domier ◽  
Terry L. Niblack ◽  
Kris N. Lambert
Keyword(s):  
Soybean Cyst Nematode ◽  
High Throughput Sequencing ◽  
Nematode Species ◽  
Cyst Nematode ◽  
Parasitic Nematodes ◽  
The Novel ◽  
Viral Pathogens ◽  
Viral Genomes ◽  
Virus Genomes ◽  
Viral Sequences

Nematodes are the most abundant multicellular animals on earth, yet little is known about their natural viral pathogens. To date, only two nematode virus genomes have been reported. Consequently, nematode viruses have been overlooked as important biotic factors in the study of nematode ecology. Here, we show that one plant parasitic nematode species, Heterodera glycines, the soybean cyst nematode (SCN), harbours four different RNA viruses. The nematode virus genomes were discovered in the SCN transcriptome after high-throughput sequencing and assembly. All four viruses have negative-sense RNA genomes, and are distantly related to nyaviruses and bornaviruses, rhabdoviruses, bunyaviruses and tenuiviruses. Some members of these families replicate in and are vectored by insects, and can cause significant diseases in animals and plants. The novel viral sequences were detected in both eggs and the second juvenile stage of SCN, suggesting that these viruses are transmitted vertically. While there was no evidence of integration of viral sequences into the nematode genome, we indeed detected transcripts from these viruses by using quantitative PCR. These data are the first finding of virus genomes in parasitic nematodes. This discovery highlights the need for further exploration for nematode viruses in all tropic groups of these diverse and abundant animals, to determine how the presence of these viruses affects the fitness of the nematode, strategies of viral transmission and mechanisms of viral pathogenesis.

scholarly journals Weeds Hosting the Soybean Cyst Nematode (Heterodera glycines Ichinohe): Management Implications in Agroecological Systems

Agronomy ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 146
Author(s):  
Leonardo F. Rocha ◽  
Karla L. Gage ◽  
Mirian F. Pimentel ◽  
Jason P. Bond ◽  
Ahmad M. Fakhoury
Keyword(s):  
Weed Management ◽  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
Integrated Management ◽  
Cropping System ◽  
Cyst Nematode ◽  
Weed Species ◽  
Initial Inoculum ◽  
Herbicide Resistant ◽  
Sites Of Action

The soybean cyst nematode (SCN; Heterodera glycines Ichinohe) is a major soybean-yield-limiting soil-borne pathogen, especially in the Midwestern US. Weed management is recommended for SCN integrated management, since some weed species have been reported to be hosts for SCN. The increase in the occurrence of resistance to herbicides complicates weed management and may further direct ecological–evolutionary (eco–evo) feedbacks in plant–pathogen complexes, including interactions between host plants and SCN. In this review, we summarize weed species reported to be hosts of SCN in the US and outline potential weed–SCN management interactions. Plants from 23 families have been reported to host SCN, with Fabaceae including most host species. Out of 116 weeds hosts, 14 species have known herbicide-resistant biotypes to 8 herbicide sites of action. Factors influencing the ability of weeds to host SCN are environmental and edaphic conditions, SCN initial inoculum, weed population levels, and variations in susceptibility of weed biotypes to SCN within a population. The association of SCN on weeds with relatively little fitness cost incurred by the latter may decrease the competitive ability of the crop and increase weed reproduction when SCN is present, feeding back into the probability of selecting for herbicide-resistant weed biotypes. Therefore, proper management of weed hosts of SCN should be a focus of integrated pest management (IPM) strategies to prevent further eco–evo feedbacks in the cropping system.

scholarly journals Prospects of Using High-Throughput Proteomics to Underpin the Discovery of Animal Host–Nematode Interactions

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 825
Author(s):  
Tao Wang ◽  
Robin Gasser
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
Anthelmintic Resistance ◽  
Nematode Species ◽  
Economic Losses ◽  
Future Research ◽  
Parasitic Nematodes ◽  
Public Health Burden ◽  
Host Parasite Interactions ◽  
Host Parasite

Parasitic nematodes impose a significant public health burden, and cause major economic losses to agriculture worldwide. Due to the widespread of anthelmintic resistance and lack of effective vaccines for most nematode species, there is an urgent need to discover novel therapeutic and vaccine targets, informed through an understanding of host–parasite interactions. Proteomics, underpinned by genomics, enables the global characterisation proteins expressed in a particular cell type, tissue and organism, and provides a key to insights at the host–parasite interface using advanced high-throughput mass spectrometry-based proteomic technologies. Here, we (i) review current mass-spectrometry-based proteomic methods, with an emphasis on a high-throughput ‘bottom-up’ approach; (ii) summarise recent progress in the proteomics of parasitic nematodes of animals, with a focus on molecules inferred to be involved in host–parasite interactions; and (iii) discuss future research directions that could enhance our knowledge and understanding of the molecular interplay between nematodes and host animals, in order to work toward new, improved methods for the treatment, diagnosis and control of nematodiases.

AAC Richard Soybean

2022 ◽  
Author(s):  
Kangfu Yu ◽  
Lorna Woodrow ◽  
M. Chun Shi
Keyword(s):  
Glycine Max ◽  
Research And Development ◽  
Soybean Cyst Nematode ◽  
Protein Concentration ◽  
Heterodera Glycines ◽  
Cyst Nematode ◽  
Processing Quality ◽  
Food Grade ◽  
High Protein Concentration ◽  
Glycine Max L

AAC Richard is a food grade soybean [Glycine max (L.) Merr] cultivar with yellow hilum, high protein concentration, and good processing quality for foreign and domestic soymilk, tofu, and miso markets. It has resistance to SCN (soybean cyst nematode) (Heterodera Glycines Ichinohe). AAC Richard was developed at the Agriculture and Agri-Food Canada (AAFC) Harrow Research and Development Centre (Harrow-RDC), Harrow, Ontario and is adapted to areas of southwest Ontario with 3100 or more crop heat units and has a relative maturity of 2.3 (MG 2.3).

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