scholarly journals RAPD Analysis for Determination of Components in Herbal Medicine

2007 ◽  
Vol 4 (s1) ◽  
pp. 21-23 ◽  
Author(s):  
V. M. Shinde ◽  
K. Dhalwal ◽  
K. R. Mahadik ◽  
K. S. Joshi ◽  
B. K. Patwardhan
Keyword(s):  
Quality Control ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Herbal Medicines ◽  
Tinospora Cordifolia ◽  
Herbal Preparation ◽  
Emblica Officinalis ◽  
Dried Fruit ◽  
Herbal Prescription

In this study, the RAPD (Random Amplified Polymorphic DNA) technique was employed for determination of the components in an Ayurvedic herbal prescription,Rasayana Churna. One-hundred-and-twenty decamer oligonucleotide primers were screened in the RAPD analysis to identify three Ayurvedic medicines, dried stem ofTinospora cordifolia, dried fruit ofEmblica officinalisand dried fruit ofTribulus terestris, the Ayurvedic prescription. Primer OPC-6 simultaneously generated three distinct amplicons, each specific to one component. The marker with 600 bp is specific toTinospora cordifolia; the marker 500 bp is specific toEmblica officinalisand the remaining marker >1000 bp was present inTribulus terestris. Presence of three herbal medicines was determined when RAPD reaction with OPC-6 was performed. The technique was proved to contribute to the identification of components in Ayurvedic herbal preparation and thus helping to serve as a complementary tool for quality control.

scholarly journals Recent Development of Quality Control Methods for Herbal Derived Drug Preparations

2018 ◽  
Vol 13 (12) ◽  
pp. 1934578X1801301 ◽  
Author(s):  
Gunawan Indrayanto
Keyword(s):  
Quality Control ◽  
Raw Materials ◽  
Herbal Medicines ◽  
Complex Nature ◽  
Chemical Marker ◽  
Chemical Profiling ◽  
Chemical Content ◽  
Pharmaceutical Industries ◽  
Microbial Toxins

Pharmaceutical industries should apply rigorous QC (quality control) to ensure the consistency, safety, and efficacy of their herbal derived drug-preparations. QC must be performed at every stage of the production line i.e. incoming raw materials, extractions, in-process control, finished products and keeping samples. Due to the complex nature of the chemical content of herbal drugs, two approaches to QC should be taken, that is quantitative determination of the selected marker(s) compound(s), and metabolite profiling. Contamination of herbal medicines by heavy metals, pesticides, toxic metabolites, microbial toxins, pathogenic microorganisms and other foreign matter should also be evaluated. A combination of chemical profiling and multivariate analysis (MVA) is recommended as the QC tool for the botanical identification method (BIM) of herbs, extracts, herb materials, and herbal drug preparations. Microscopic methods, DNA profiling or chemical marker(s) are not recommended for use as the sole BIM due to the lack of specificity. Only markers that meet certain criteria i.e. quality active (QA) markers can be utilized as a QC tool. The limit specification range of markers used as QC tools should be described in the analytical target profile (ATP). To gain reliable results of any analysis that has been performed at any QC laboratory, the analysis method must be validated according to the newest guidance. Sample detection limit of any toxic compound(s) should be lower than its cut-off value and MPL. The reliability of any results of analysis of a QC laboratory must be evaluated by using QC-samples for each series of measurements.

Determination of heavy metals for the quality control in argentinian herbal medicines by ETAAS and ICP-OES

2007 ◽  
Vol 45 (6) ◽  
pp. 1060-1064 ◽  
Author(s):  
María R. Gomez ◽  
Soledad Cerutti ◽  
Lorena L. Sombra ◽  
María F. Silva ◽  
Luis D. Martínez
Keyword(s):  
Heavy Metals ◽  
Quality Control ◽  
Herbal Medicines ◽  
Icp Oes

Genotoxic evaluation of newly synthesized iminothiazolidinones

2017 ◽  
Vol 33 (11) ◽  
pp. 811-820
Author(s):  
Fatma Tulay Tugcu ◽  
Kadir Turhan ◽  
Mehmet Karadayi ◽  
Medine Gulluce
Keyword(s):  
Seed Germination ◽  
Rapd Analysis ◽  
Mutagenic Activity ◽  
Random Amplified Polymorphic Dna ◽  
E Coli ◽  
Toxicological Effects ◽  
Actual Use ◽  
Genotoxic Compounds ◽  
Test Materials

The current study was designed to assess the potential toxicological effects of newly synthesized iminothiazolidinones by employing Ames Salmonella, Escherichia coli WP2, Zea mays seed germination, and random amplified polymorphic DNA (RAPD) assay systems. The bacterial tester strains S. typhimurium TA1535, TA1537, TA1538, TA98, TA100, and E. coli WP2 uvrA were chosen to test the direct gene mutation inducing capabilities of the test materials in prokaryotic systems and Z. mays seeds for determination of potential toxicological effects in eukaryotic systems. OPA-3 and OPA-6 primers were used in the RAPD analysis to determine genotoxic activities on the eukaryotic genomes. According to the results, none of the test materials showed significant mutagenic activity on the bacterial tester strains at the chosen concentrations. Additionally, none of the tested compounds showed inhibition of the germination of Z. mays seeds. In contrast, the RAPD analysis results were inconsistent with the bacterial reversion assays and the seed germination assay results. All test materials significantly changed the RAPD profiles for OPA-3; however, only compound 5 showed a significant change for OPA-6 when compared with the control groups. In conclusion, the newly synthesized iminothiazolidinone derivatives (C1–C5) were determined as potentially genotoxic compounds and they should be checked with multiple toxicology test systems before further studies to determine their actual use.

scholarly journals HPLC–PDA and LC–MS/MS Analysis for the Simultaneous Quantification of the 14 Marker Components in Sojadodamgangki-Tang

2020 ◽  
Vol 10 (8) ◽  
pp. 2804 ◽  
Author(s):  
Chang-Seob Seo ◽  
Mee-Young Lee
Keyword(s):  
Quality Control ◽  
Liquid Chromatography ◽  
High Performance ◽  
Herbal Medicines ◽  
Photodiode Array Detection ◽  
Liquid Chromatography Tandem Mass ◽  
Photodiode Array ◽  
Array Detection ◽  
Ancient Times

Sojadodamgangki-tang (SDGT) is a traditional Korean medicine consisting of 12 medicinal herbs that has been used in Korea for the treatment of asthma since ancient times. However, the quality control of herbal formulas that contain two or more herbal medicines remains challenging. In this study, 14 marker components were analyzed simultaneously by using high-performance liquid chromatography with photodiode array detection in addition to the use of liquid chromatography–tandem mass spectrometry for quality evaluation of SDGT. The simultaneous determination of the 14 marker components was validated in terms of linearity, recovery, and precision. The established methods can provide useful data for the quality control of SDGT and related herbal formulas.

scholarly journals Actual Aspects of Quality Control and Standardization of Rosehip Fruits

2021 ◽  
Vol 10 (3) ◽  
pp. 167-175
Author(s):  
D. A. Zhdanov ◽  
V. A. Kurkin ◽  
V. B. Braslavsky ◽  
A. I. Agapov
Keyword(s):  
Quality Control ◽  
Ascorbic Acid ◽  
High Performance ◽  
Herbal Medicines ◽  
Biologically Active ◽  
Phytochemical Study ◽  
Study Results ◽  
Vis Spectroscopy ◽  
The Republic

Introduction. Currently, herbal medicines are becoming increasingly popular. In this regard, of particular interest are medicinal herbal preparations based on rosehip fruits, which have a tonic, choleretic and wound healing effect due to the presence of ascorbic acid, flavonoids and carotenoids. According to the State Pharmacopoeia of the Russian Federation (SP RF) of the XIV edition, the determination of the main groups of biologically active substances (BAS) by thin-layer chromatography (TLC) is carried out only by the presence of ascorbic acid, while the sensitivity of the method does not allow it to be detected in rosehip fruits of low-vitamin species.Aim. Aim is the development of new approaches to control quality and standardization of Rosehip fruits with using of modern pharmacognostic analysis methods.Materials and methods. Prepared in Samara Region, the Republic of Mari El Rosehip fruits samples in 2020 and commercial samples from various manufacturers. TLC, UV/Vis-spectroscopy and high-performance liquid chromatography (HPLC) methods were used for determination the main BAS groups.Results and discussion. The introduction of flavonoids as one of the definitioned groups of BAS in rosehip fruits along with ascorbic acid was justified. A technique for the identification of ascorbic acid by UV/Vis-spectroscopy (wavelength 264 ± 2 nm) was developed and shown the determination possibility of this substance by HPLC.Conclusion. So phytochemical study results the expediency of the using of modern approaches to quality control and standardization of rosehip fruits, consisting in the qualitative determination of ascorbic acid by UV/Vis-spectroscopy and HPLC, as well as rutin by TLC, was justified.

scholarly journals 011 RAPD ANALYSIS IDENTIFIES GENETIC VARIATION AND RELATEDNESS WITHIN THE CRANBERRY VARIETY MCFARLIN

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 429b-429
Author(s):  
Richard Novy ◽  
Nicholi Vorsa ◽  
Kim Patten
Keyword(s):  
Cluster Analysis ◽  
Genetic Variation ◽  
Genetic Basis ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Washington State ◽  
Genetic Component ◽  
Morphological Descriptors ◽  
Related Individuals

The cranberry cultivar `HcFarlin', selected from a natural bog in Massachusetts in 1874, has become the most widely grown cultivar in the Northwestern U.S.A. Washington state growers have noted variable productivity among `McFarlin' bogs. The determination of whether there is a genetic basis for the variability has been made difficult by a paucity of reliable morphological descriptors in cranberry. A random amplified polymorphic DNA (RAPD) analysis of 45 clones sampled from 12 WA `McFarlin' bogs identified 17 unique RAPD profiles. Cluster analysis identified 7 groups having various numbers of distinct, but related individuals. Eight clones were found to have RAPD profiles identical to the cultivar `Howes' indicating varietal misclassification had occurred in some bogs. One group of clones that originated from bogs classified as “Good” or “True” Mcfarlin' by growers had RAPD profiles similar to those of representatives from WI and MA `Mcfarlin' bogs. RAPD analysis has shown that `McFarlin' is represented by several genotypes, suggesting that the observed variability in production may have a genetic component.

scholarly journals Reference Standards Based on the Grosheimin and Cynaropicrin

2016 ◽  
Vol 18 (1) ◽  
pp. 73
Author(s):  
A.S. Adekenova
Keyword(s):  
Quality Control ◽  
High Performance ◽  
Sesquiterpene Lactones ◽  
Herbal Medicines ◽  
Analysis Data ◽  
Reference Standards ◽  
Normative Documents ◽  
The Republic ◽  
Pharmaceutical Production

The aim of this work is the development of effective method for producing the reference standards of Grosheimin and Cynaropicrin for quality control of domestic herbal medicines, as well as project development concerning normative documents on reference standards of Grosheimin and Cynaropicrin, an introduction of reference standards to the Pharmacopoeia of Kazakhstan. This article discusses the method of allocation and purification of reference standards of Grosheimin and Cynaropicrin which are sesquiterpene lactones of guaiane type were obtained from the ethyl acetate extract of <em>Chartolepis intermedia</em> Boiss., using centrifugal distribution chromatography and high performance liquid chromatography, allows to obtain the qualitative target products. The effectiveness of developed technology has reduced the labor contribution in 2.0 times and as a consequence the prime cost of end products come down 3 times. Using of Grosheimin and Cynaropicrin as own<br />reference standard of the Republic of Kazakhstan for monitoring of staged quality control of pharmaceutical production: access control, intermediate control and output control, it means the finished dosage form. There are some chromatographic analysis data of the samples. Such methods as IR and NMR- spectophotometries were applied for determination of molecular structure. Pharmacopoeial methods were used to determine the color, taste, odor and solubility of reference standards in various solvents. Projects for temporary Analytical Normative Documents on reference standards of Grosheimin and Cynaropicrin were developed in accordance with requirement of State Pharmacopeia of the Republic of Kazakhstan. On the basis of obtained results the database for State Pharmacopoeia of the Republic of Kazakhstan will be formed, as well as control over production of new pharmaceuticals from <em>Chartolepis intermedia</em> Boiss. and <em>Saussurea salsa</em> (Pall.) Spreng.

UV spectroscopic method for determination of phenytoin in bulk and injection forms

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Quality Control ◽  
Spectrophotometric Method ◽  
Spectroscopic Method ◽  
Ich Guidelines ◽  
Precision And Accuracy ◽  
Routine Quality Control

Recent study was conducted to develop a simple UV spectrophotometric method to determine Phenytoin in bulk and injection form according to official requirement and validate as per ICH guidelines. λmax of Phenytoin was found 202 nm. Linearity existed perceived in the concentration assortment 2-8 μg/ml (r2 = 0.999) for the method. The method was validated pertaining to linearity, precision and accuracy studies, LOD and LOQ consistent with ICH guidelines. The existent method was establish to be simple, linear, precise, accurate as well as sensitive and can be applied for routine quality control enquiry for the analysis of Phenytoin in bulk and injection form.

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