scholarly journals Low-moisture food matrices as probiotic carriers

2019 ◽  
Vol 366 (Supplement_1) ◽  
pp. i49-i59
Author(s):  
Martín Sebastián Marcial-Coba ◽  
Susanne Knøchel ◽  
Dennis Sandris Nielsen
Keyword(s):  
Storage Conditions ◽  
Factors Associated ◽  
Desiccation Process ◽  
The Matrix ◽  
Food Matrices ◽  
Near Future ◽  
And Storage ◽  
Upper Gastrointestinal ◽  
Selection Of

ABSTRACT To exert a beneficial effect on the host, adequate doses of probiotics must be administered and maintaining their viability until consumption is thus essential. Dehydrated probiotics exhibit enhanced long-term viability and can be incorporated into low-moisture food matrices, which also possess high stability at refrigeration and ambient temperature. However, several factors associated with the desiccation process, the physicochemical properties of the matrix and the storage conditions can affect probiotic survival. In the near future, an increased demand for probiotics based on functionally dominant members of the gut microbiome (‘next-generation probiotics’, NGP) is expected. NGPs are very sensitive to oxygen and efficient encapsulation protocols are needed. Strategies to improve the viability of traditional probiotics and particularly of NGPs involve the selection of a suitable carrier as well as proper desiccation and protection techniques. Dehydrated probiotic microcapsules may constitute an alternative to improve the microbial viability during not only storage but also upper gastrointestinal tract passage. Here we review the main dehydration techniques that are applied in the industry as well as the potential stresses associated with the desiccation process and storage. Finally, low- or intermediate-moisture food matrices suitable as carriers of traditional as well as NGPs will be discussed.

Should phosphatidylethanol be currently analysed using whole blood, dried blood spots or both?

2019 ◽  
Vol 57 (5) ◽  
pp. 617-622 ◽  
Author(s):  
Van Long Nguyen ◽  
Michael Fitzpatrick
Keyword(s):  
Whole Blood ◽  
Blood Cells ◽  
Dried Blood Spots ◽  
Storage Conditions ◽  
Ethanol Metabolism ◽  
Clinical Use ◽  
Blood Spots ◽  
Comparative Review ◽  
And Storage

Abstract Phosphatidylethanol (PEth) are phospholipids produced through non-oxidative ethanol metabolism. They accumulate in red blood cells and have been traditionally analysed in whole blood as potential biomarkers for moderate to long-term alcohol consumption. More recently, their analysis in dried blood spots has been gaining favour, namely, due to the ease in sampling, transport and storage conditions required. This paper aims at providing a short comparative review between analysing PEth in whole blood and dried blood spots and the potential pitfalls that researchers may face when setting up PEth testing for clinical use.

LNG pricing trends and key considerations

2015 ◽  
Vol 55 (2) ◽  
pp. 417
Author(s):  
Jacques van Rhyn ◽  
Janelle Sadri
Keyword(s):  
Transfer Pricing ◽  
Asia Pacific ◽  
Asia Pacific Region ◽  
Trade Patterns ◽  
Market Situation ◽  
Competitive Prices ◽  
Speculative Trading ◽  
Near Future ◽  
Selection Of

Historically, LNG has been sold through long-term contracts with limited flexibility in volume and price. LNG trade patterns have evolved significantly, adding to increased sales of multiple cargoes on the spot market, brokered trades and speculative trading positions being taken up by non-traditional players. Buyers in the Asia-Pacific region are keen to secure supply for local markets, while Australian producers, particularly subsidiaries of foreign headquartered groups, are under pressure to sell at competitive prices. From a transfer pricing perspective, the Australian Taxation Office (ATO) has placed increased scrutiny on the commerciality of arrangements, arm’s length outcomes and profit allocations between Australian taxpayers and their international related parties (e.g. marketing and trading hubs). This extended abstract covers: factors that could impact on the selection of a price index and the slope or gradient to be applied in pricing formulae; blended pricing based on an average of different indices, and why pooling and trading may make commercial sense, although revenue authorities may not look favourably upon it; the importance of the contractual terms, the market situation and the other commercial contract conditions on the pricing of related-party LNG sales; and, the value in potentially seeking an advanced pricing agreement in a related party LNG pricing context, given the significance of the transactions. Given the practical and commercial challenges facing the industry and with several projects commencing production in the relatively near future, this is very topical. The authors use case studies to illustrate the key concepts.

CURRENT BEST PRACTICES AND BIOBANKING RECOMMENDATIONS

2019 ◽  
Vol 64 (12) ◽  
pp. 769-776 ◽  
Author(s):  
Y. V. Doludin ◽  
A. L. Borisova ◽  
M. S. Pokrovskaya ◽  
O. V. Stefanyuk ◽  
O. V. Sivakova ◽  
...  
Keyword(s):  
Best Practices ◽  
Preanalytical Phase ◽  
Wide Range ◽  
Research Problems ◽  
Processing And Storage ◽  
And Storage ◽  
Quality Of Research ◽  
Selection Of

The biobank is a structure established with the goal of long-term responsible storage of biological samples and the associated data for their further use in scientific and clinical research. The objectives of biobanking are the creation of unified recommendations on: the planning of premises and the selection of equipment for storage; development of management methods and staff training; standardization of methods for the collection, shipping, processing and storage of biomaterial of various origins, as well as methods for quality control and validation of the applied methods; creation and use of databases of information accompanying biospecimens. The lack of common standards for conducting the preanalytical phase has been the cause of low accuracy and poor reproducibility of research results. To date, a large number of guidelines and best practices have been published that provide an answer to a wide range of problems in organizing the biobanking process. The article provides an overview of the most famous biobanking guidelines that can be used to solve various research problems. Biobanking in Russia is actively developing. Since 1996 there is a work on the legislative regulation of biobanking activities, as a result of which a number of regulatory documents have been issued. An important stage in the development of biobanking in Russia was the establishment of the “National Association of Biobanks and Biobanking Specialists” (NASBio) in 2018, which included representatives of medical and research institutions, commercial firms, and qualified specialists in the field of biobanking. One of the key tasks of NASBio is the adaptation and implementation of the best biobanking practices in Russian research institutes and centers. The use of modern guidelines and best practices on biobanking will lead to an increase in the quality of research and publications.

scholarly journals Preservation of Azotobacter chroococcum vegetative cells in dry polymers

2014 ◽  
Vol 20 (2) ◽  
pp. 201 ◽  
Author(s):  
Daniel Rojas-Tapias ◽  
Oriana Ortega Sierra ◽  
Diego Rivera Botía ◽  
Ruth Bonilla
Keyword(s):  
Storage Conditions ◽  
Azotobacter Chroococcum ◽  
Bacterial Degradation ◽  
Protective Agent ◽  
Degradation Rates ◽  
Different Temperatures ◽  
And Storage ◽  
Temperature Time ◽  
Long Term Preservation

We studied the preservation of Azotobacter chroococcum C26 using three dry polymers: carrageenin, sodium alginate, and HPMC, using a method of accelerated degradation. Bacterial viability, as response variable, was measured at three temperatures in four different times, which was followed by calculation of bacterial degradation rates. Results showed that temperature, time of storage, and protective agent influenced both viability and degradation rates (P;lt;0.05). We observed, using the Arrhenius thermodynamic model, that the use of polymers increased the activation energy of bacterial degradation compared to control. We obtained thermodynamic models for each polymer, based on the Arrhenius equation, which predicted the required time for thermal degradation of the cells at different temperatures. Analysis of the models showed that carrageenin was the best polymer to preserve A. chroococcum C26 since ~ 900 days are required at 4 ºC to reduce its viability in two log units. We conclude, therefore, that long-term preservation of A. chroococcum C26 using dry polymers is suitable under adequate preservation and storage conditions.

scholarly journals ANTIOXIDANT PROPERTIES AND FRUIT QUALITY DURING LONG-TERM STORAGE OF “ROCHA” PEAR: EFFECTS OF MATURITY AND STORAGE CONDITIONS

2010 ◽  
Vol 33 (1) ◽  
pp. 1-20 ◽  
Author(s):  
FILIPE J.P. SILVA ◽  
MARIA HELENA GOMES ◽  
FERNANDA FIDALGO ◽  
JOSÉ A. RODRIGUES ◽  
DOMINGOS P.F. ALMEIDA
Keyword(s):  
Fruit Quality ◽  
Antioxidant Properties ◽  
Storage Conditions ◽  
Long Term Storage ◽  
And Storage ◽  
Term Storage

Selection of External Coatings for Northern Pipelines: Laboratory Methodologies for Evaluation and Qualification of Coatings

2004 ◽  
Author(s):  
Sankara Papavinasam ◽  
R. Winston Revie ◽  
Michael Attard
Keyword(s):  
Standardized Tests ◽  
Protective Coatings ◽  
Climatic Conditions ◽  
Operational Conditions ◽  
The North ◽  
Long Term Performance ◽  
Term Performance ◽  
Near Future ◽  
Selection Of

In the near future, the construction of northern pipelines for transmission of natural gas will begin in North America. Construction in the harsh northern climate, with temperatures as low as −45°C, and remote location will impose unique challenges with respect to protective coatings. It is critical that the design of coatings be adequate to protect the pipelines under long-term, severe environmental conditions, including the extreme climatic conditions that will apply in the North before the pipe is installed and operation begins. There are many quality coatings from which to choose for application on new pipelines. The main issue is in understanding how to select and use coatings on pipelines in new regimes (e.g. Northern pipelines), which may operate in a different environment than do existing pipelines. Uniform, standardized tests that would simulate the conditions during construction and operation of Northern pipelines will allow external pipeline coatings to be selected with confidence regarding anticipated long-term performance under operational conditions. Selection of mainline coatings is important, but there is also a need to focus on field-applied coatings for both repairs and joints. Methodologies and standards that are available to evaluate coatings are reviewed in this paper.

Response of Longleaf Pine Seeds to Storage Conditions and Pregermination Treatments

1993 ◽  
Vol 17 (4) ◽  
pp. 174-179 ◽  
Author(s):  
James P. Barnett ◽  
John P. Jones
Keyword(s):  
Seed Quality ◽  
Longleaf Pine ◽  
Pinus Palustris ◽  
Storage Conditions ◽  
Operational Conditions ◽  
Long Term Storage ◽  
Processing And Storage ◽  
And Storage ◽  
Term Storage

Abstract Although longleaf pine (Pinus palustris Mill.) seeds are considered the most susceptible of the southern pines to damage during collection, processing, and storage, results of these studies show that high seed quality can be assured for periods up to 20 yr through proper handling and storing techniques. Recommendations for long-term storage include drying seeds to moisture contents of 10% or less and storing at subfreezing temperatures, preferably near 0°F. Reevaluation of stratification treatments applied under operational conditions indicates that the soaking in water that is necessary for seed imbibition reduces total germination in an amount proportional to the length of the soak. Stratification is not recommended except under very controlled conditions. South. J. Appl. For. 17(4):174-179.

scholarly journals Environment is crucial to the cooking time of beans

2012 ◽  
Vol 32 (3) ◽  
pp. 573-578 ◽  
Author(s):  
Bruna Arruda ◽  
Altamir Frederico Guidolin ◽  
Jefferson Luís Meirelles Coimbra ◽  
Jaqueline Battilana
Keyword(s):  
Storage Time ◽  
Storage Condition ◽  
Storage Conditions ◽  
Cooking Time ◽  
Dependent Manner ◽  
Simple Linear Regression ◽  
Long Term Storage ◽  
And Storage ◽  
Term Storage

The objectives of this study were to understand how genotype, storage time, and storage conditions affect cooking time of beans and to indicate storage techniques that do not affect the cooking time. The grains were subjected to five different storage periods and six different storage conditions. The cooking time was estimated using the Mattson Cooker. The data were subjected to analysis of variance and a subsequent adjustment of simple linear regression for deployment of the interactions between the factors. Contrasts were used to determine the best levels of the factor storage condition. Genotype did not impact cooking time when the storage time and storage conditions were considered. Time and storage conditions affect the cooking time of beans in a dependent manner, but time of storage had the biggest influence. The best conditions for long-term storage of beans ensuring a smaller increase in cooking time is plastic storage at low temperatures. Thus, plastic freezer storage is a practical alternative for consumers.

scholarly journals Measurable Cytokine Concentrations in Pig Seminal Plasma Are Modified by Semen Handling and Storage

Biology ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 276
Author(s):  
Lorena Padilla ◽  
Isabel Barranco ◽  
Inmaculada Parrilla ◽  
Xiomara Lucas ◽  
Heriberto Rodriguez-Martinez ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Biological Fluids ◽  
Storage Conditions ◽  
Reliable Measurement ◽  
Short Term ◽  
Gm Csf ◽  
Long Term Storage ◽  
And Storage ◽  
Term Storage

Sample handling and storing are critical steps for the reliable measurement of circulating biomolecules in biological fluids. This study evaluates how cytokine measurements in pig seminal plasma (SP) vary depending on semen handling and SP storage. Thirteen cytokines (GM-CSF, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-18 and TNFα) were measured using Luminex xMAP® technology in individual seminal plasma (SP) samples (n = 62) from healthy breeding boars. Three separate experiments explored the delay (2 h and 24 h) in SP collection after ejaculation (Experiment 1) and SP storage, either short-term (5 °C, −20 °C and −80 °C for 72 h, Experiment 2) or long-term (at −20 °C and −80 °C for two months, Experiment 3), before analysis. Levels in fresh SP-samples were used as baseline control values. Delays in SP harvesting of up to 24 h did not substantially impact SP cytokine measurements. Some cytokines showed instability in stored SP samples, mainly in long-term storage. Ideally, cytokines in pig SP should be measured in fresh samples harvested within 24 h after ejaculation. If storage of SP is imperative, storage conditions should be adjusted for each cytokine.

scholarly journals Groundwater Laws and Regulations: A Preliminary Survey of Thirteen U.S. States (Second Edition)

2020 ◽  
Author(s):  
Alexander Bennett ◽  
Contessa Gay ◽  
Ashley Graves ◽  
Thomas Long ◽  
Erin Milliken ◽  
...  
Keyword(s):  
The United States ◽  
Law School ◽  
Water Law ◽  
Preliminary Survey ◽  
Legal Regime ◽  
The Matrix ◽  
Student Researchers ◽  
Near Future ◽  
Selection Of ◽  
Laws And Regulations

This report presents results of a study investigating the groundwater laws and regulations of thirteen U.S. states. The report is actually the second edition of the study following amendments made to the first edition in response to extensive feedback and reviews solicited from practitioners, academics, and other professionals working in the field of water law from across the country. The purpose of the project is to compile and present the groundwater laws and regulations of every state in the United States that could then be used in a series of comparisons of groundwater governance principles, strategies, issues, and challenges. Professor Gabriel Eckstein at Texas A&M University School of Law and Professor Amy Hardberger at Saint Mary’s University Law School developed a matrix to ascertain chief components and characteristics of the groundwater legal regime of each state. Student researchers then used the matrix to respond to a standardized set of questions about the groundwater laws and regulations of a selection of states. In the near future, additional volumes with surveys of other U.S. states will be issued.

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