scholarly journals INTEGRATION EFFICIENCY IN DNA-INDUCED TRANSFORMATION OF PNEUMOCOCCUS. I. A METHOD OF TRANSFORMATION IN SOLID MEDIUM AND ITS USE FOR ISOLATION OF TRANSFORMATION-DEFICIENT AND RECOMBINATION-MODIFIED MUTANTS

Genetics ◽  
1973 ◽  
Vol 75 (1) ◽  
pp. 23-33
Author(s):  
Gerard Tiraby ◽  
Jean-Pierre Claverys ◽  
Michel Armand Sicard
Keyword(s):  
Ultraviolet Light ◽  
High Efficiency ◽  
Solid Medium ◽  
Transformation Efficiency ◽  
Replica Plating ◽  
Low Efficiency ◽  
Integration Efficiency

ABSTRACT A method of transformation on solid medium especially adapted for pneumococcus has been developed. Under specific conditions, all colonies that are allowed to grow in the presence of transforming DNA for six hours give rise to transformed bacteria. Combined with replica plating this technique has been used to isolate mutants modified with regard to recombination. Most of the mutants found are transformation-defective and show a large diversity in their response to ultraviolet light. Some of these mutants have lost their ability to take up transforming DNA. One shows a reduced yield of transformants for a given quantity of DNA taken up. Mutants that manifest altered behavior with regard to marker efficiencies have also been isolated. One of these exhibits a decrease in the transformation efficiency of only the high efficiency markers and two mutants show a decrease in the transformation efficiency of the low efficiency markers.

INTEGRATION EFFICIENCY IN DNA-INDUCED TRANSFORMATION OF PNEUMOCOCCUS. II. GENETIC STUDIES OF MUTANT INTEGRATING ALL THE MARKERS WITH A HIGH EFFICIENCY

Genetics ◽  
1973 ◽  
Vol 75 (1) ◽  
pp. 35-48
Author(s):  
Gérard Tiraby ◽  
Michel Armand Sicard
Keyword(s):  
Ultraviolet Light ◽  
Type Strain ◽  
Wild Type Strain ◽  
High Efficiency ◽  
Wild Type ◽  
Genetic Studies ◽  
X Ray ◽  
Reference Marker ◽  
Low Efficiency ◽  
Integration Efficiency

ABSTRACT Transformation of the pneumococcus mutant 401 by DNA's bearing the standard reference marker and several other markers belonging to two unlinked loci has shown that differences in the integration efficiencies of these markers were considerably reduced in this strain compared to the wild-type strain Cl  3. The sensitivities of mutant 401 to ultraviolet light and to X-ray irradiation are the same as those of Cl  3. However, in 401 all the markers tested are more resistant to inactivation as shown by transformation of 401 and Cl  3 by ultraviolet-irradiated DNA. The increase in resistance is greater for low efficiency (LE) markers than for high efficiency (HE) markers.—The decreased discrimination between LE and HE markers in strain 401 is not due to a mechanism related to modification of markers in the transforming DNA by the recipient cells, nor are the proteins inducing competence of the cells responsible for the differences in the integration efficiencies of various markers.—Genetic studies of the fate of recombinants as well as the measure of the amount of DNA taken up have shown that all the markers are integrated in strain 401 by the same recombination process, that specific to high efficiency markers.

scholarly journals CHROMOSOMAL BASIS OF THE MEROZYGOSITY IN A PARTIALLY DIPLOID MUTANT OF PNEUMOCOCCUS

Genetics ◽  
1975 ◽  
Vol 80 (4) ◽  
pp. 667-678
Author(s):  
Mary Lee S Ledbetter ◽  
Rollin D Hotchkiss
Keyword(s):  
High Efficiency ◽  
Point Mutations ◽  
Resistant Mutant ◽  
Chromosomal Marker ◽  
Structural Abnormality ◽  
C Cells ◽  
Wild Type ◽  
Chromosomal Locus ◽  
Low Efficiency ◽  
Derivatives Of

ABSTRACT A sulfonamide-resistant mutant of pneumococcus, sulr-c, displays a genetic instability, regularly segregating to wild type. DNA extracts of derivatives of the strain possess transforming activities for both the mutant and wild-type alleles, establishing that the strain is a partial diploid. The linkage of sulr-c to strr-61, a stable chromosomal marker, was established, thus defining a chromosomal locus for sulr-c. DNA isolated from sulr-c cells transforms two mutant recipient strains at the same low efficiency as it does a wild-type recipient, although the mutant property of these strains makes them capable of integrating classical "low-efficiency" donor markers equally as efficiently as "high efficiency" markers. Hence sulr-c must have a different basis for its low efficiency than do classical low efficiency point mutations. We suggest that the DNA in the region of the sulr-c mutation has a structural abnormality which leads both to its frequent segregation during growth and its difficulty in efficiently mediating genetic transformation.

scholarly journals A Brief Review on Solvent-Free Synthesis of Zeolites

Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 788
Author(s):  
Jinlin Mei ◽  
Aijun Duan ◽  
Xilong Wang
Keyword(s):  
High Efficiency ◽  
Raw Materials ◽  
Solvent Free ◽  
High Yield ◽  
Control Synthesis ◽  
Mechanical Mixing ◽  
Synthesis Mechanism ◽  
Wastewater Pollution ◽  
Low Efficiency ◽  
Solvent Free Synthesis

The traditional hydrothermal method to prepare zeolite will inevitably use a large amount of water as a solvent, which will lead to higher autogenous pressure, low efficiency, and wastewater pollution. The solvent-free method can be used to synthesize various types of zeolites by mechanical mixing, grinding, and heating of solid raw materials, which exhibits the apparent advantages of high yield, low pollution, and high efficiency. This review mainly introduces the development process of solvent-free synthesis, preparation of hierarchical zeolite, morphology control, synthesis mechanism and applications of solvent-free methods. It can be believed that solvent-free methods will become a research focus and have enormous industrial application potential.

scholarly journals Inflight Transmission of COVID-19 Based on Experimental Aerosol Dispersion Data

2021 ◽  
Author(s):  
Zhaozhi Wang ◽  
Edwin R Galea ◽  
Angus Grandison ◽  
John Ewer ◽  
Fuchen Jia
Keyword(s):  
High Efficiency ◽  
Face Mask ◽  
Generation Rate ◽  
Aviation Industry ◽  
Filtration System ◽  
Dispersion Data ◽  
Low Probability ◽  
Meal Service ◽  
Low Efficiency ◽  
Aerosol Dispersion

Abstract Background An issue of concern to the travelling public is the possibility of in-flight transmission of COVID-19 during long- and short-haul flights. The aviation industry maintains that the probability of contracting the illness is small based on reported cases, modelling and data from aerosol dispersion experiments conducted on-board aircraft. Methods Using experimentally derived aerosol dispersion data for a B777–200 aircraft and a modified version of the Wells-Riley equation we estimate inflight infection probability for a range of scenarios involving quanta generation rate and face mask efficiency. Quanta generation rates were selected based on COVID-19 events reported in the literature while mask efficiency was determined from the aerosol dispersion experiments. Results The MID-AFT cabin exhibits the highest infection probability. The calculated maximum individual infection probability (without masks) for a 2-hour flight in this section varies from 4.5% for the ‘Mild Scenario’ to 60.2% for the ‘Severe Scenario’ although the corresponding average infection probability varies from 0.1% to 2.5%. For a 12-hour flight, the corresponding maximum individual infection probability varies from 24.1% to 99.6% and the average infection probability varies from 0.8% to 10.8%. If all passengers wear face masks throughout the 12-hour flight, the average infection probability can be reduced by approximately 73%/32% for high/low efficiency masks. If face masks are worn by all passengers except during a one-hour meal service, the average infection probability is increased by 59%/8% compared to the situation where the mask is not removed. Conclusions This analysis has demonstrated that while there is a significant reduction in aerosol concentration due to the nature of the cabin ventilation and filtration system, this does not necessarily mean that there is a low probability or risk of in-flight infection. However, mask wearing, particularly high-efficiency ones, significantly reduces this risk.

scholarly journals High Efficiency Generalized Parallel Counters for Look-Up Table Based FPGAs

2015 ◽  
Vol 2015 ◽  
pp. 1-16 ◽  
Author(s):  
Burhan Khurshid ◽  
Roohie Naaz Mir
Keyword(s):  
Power Dissipation ◽  
High Speed ◽  
High Efficiency ◽  
Critical Path ◽  
Fir Filters ◽  
Path Delay ◽  
Look Up Table ◽  
Improved Performance ◽  
Ip Cores ◽  
Low Efficiency

Generalized parallel counters (GPCs) are used in constructing high speed compressor trees. Prior work has focused on utilizing the fast carry chain and mapping the logic onto Look-Up Tables (LUTs). This mapping is not optimal in the sense that the LUT fabric is not fully utilized. This results in low efficiency GPCs. In this work, we present a heuristic that efficiently maps the GPC logic onto the LUT fabric. We have used our heuristic on various GPCs and have achieved an improvement in efficiency ranging from 33% to 100% in most of the cases. Experimental results using Xilinx 5th-, 6th-, and 7th-generation FPGAs and Stratix IV and V devices from Altera show a considerable reduction in resources utilization and dynamic power dissipation, for almost the same critical path delay. We have also implemented GPC-based FIR filters on 7th-generation Xilinx FPGAs using our proposed heuristic and compared their performance against conventional implementations. Implementations based on our heuristic show improved performance. Comparisons are also made against filters based on integrated DSP blocks and inherent IP cores from Xilinx. The results show that the proposed heuristic provides performance that is comparable to the structures based on these specialized resources.

Building Self-Service Printing System on College Educational Administration

2014 ◽  
Vol 889-890 ◽  
pp. 1262-1265
Author(s):  
Chun Fang Shi
Keyword(s):  
Educational Administration ◽  
High Efficiency ◽  
Ic Card ◽  
Hardware System ◽  
Teachers And Students ◽  
Human Cost ◽  
Printing System ◽  
Background Database ◽  
Self Service ◽  
Low Efficiency

Printing system of college educational administration has been managed by artificial, which result in low efficiency and high human cost. So we build a system by docking the software and hardware system of background database based on IC card to realize self-service printing for teachers and students of college, which provide a high-efficiency, convenient and humanized service mode, and enhane the economical sense of teachers and students for creating a green campus.

Carbon dioxide requirement of Neisseria gonorrhoeae growing on a solid medium

1976 ◽  
Vol 4 (2) ◽  
pp. 129-132
Author(s):  
D J Platt
Keyword(s):  
Carbon Dioxide ◽  
Neisseria Gonorrhoeae ◽  
Rapid Growth ◽  
Solid Medium ◽  
Colony Growth ◽  
Growth Cycle ◽  
Lag Phase ◽  
Replica Plating ◽  
Early Stages ◽  
Male Patients

A survey of 120 isolations of Neisseria gonorrhoeae from male patients showed that 47 (39%) isolates were unable to produce visible colonies without a supply of exogenous carbon dioxide. Of 63 strains, 25 strains required CO2 for isolation but none exhibited a CO2 requirement beyond subculture 4. CO2-requiring strains deprived of CO2 appeared to lose their colony-forming ability exponentially in an environment otherwise conductive to growth. The CO2 requirement was found to be linked to the initiation of growth. An agar-to-agar replica-plating device was used to study the early stages of colonial growth. The CO2 requirement was also found to correlate with the various phases of the colony growth cycle, such that it was required during lag phase, not required during the phase of rapid growth, and returned as colonies aged. These results are discussed.

Indications for Dialysis in Acute Kidney Injury

2020 ◽  
Author(s):  
Ankit Patel ◽  
Kenneth B Christopher
Keyword(s):  
Acute Kidney Injury ◽  
Renal Replacement Therapy ◽  
Replacement Therapy ◽  
High Efficiency ◽  
Kidney Injury ◽  
Intermittent Hemodialysis ◽  
Continuous Venovenous Hemofiltration ◽  
Continuous Venovenous Hemodialysis ◽  
Low Efficiency ◽  
The Impact

Renal replacement therapy (RRT) can be used to support patient’s kidney function in cases of acute kidney injury (AKI). However, timing, modality, and dosing of RRT continue to remain in question. Recent studies have begun to provide data to help guide clinicians on when to initiate RRT, what form of RRT to use ranging from continuous venovenous hemofiltration (VVH) to intermittent hemodialysis, and the impact of high versus low-intensity dosing. Additionally, the risks associated with temporary vascular access with regard to thrombosis and infection, the impact of high efficiency and flux versus low efficiency and flux membranes, and options for anticoagulation in RRT for AKI are also discussed. This review contains 75 references.  Key words: acute kidney injury, chronic kidney disease, continuous venovenous hemofiltration, continuous venovenous hemodialysis, renal replacement therapy, venovenous hemofiltration, 

scholarly journals Insights from Past Trends in Exergy Efficiency and Carbon Intensity of Electricity: Portugal, 1900–2014

Energies ◽  
2019 ◽  
Vol 12 (3) ◽  
pp. 534
Author(s):  
Laura Felício ◽  
Sofia T. Henriques ◽  
André Serrenho ◽  
Tiago Domingos ◽  
Tânia Sousa
Keyword(s):  
World War I ◽  
High Efficiency ◽  
Electricity Production ◽  
Transport Sector ◽  
Carbon Intensity ◽  
Renewable Electricity ◽  
World War ◽  
Residential Sector ◽  
Electricity Use ◽  
Low Efficiency

We use the societal exergy analysis to identify periods and factors controlling efficiency dilution and carbon deepening of electricity in Portugal from 1900 to 2014. Besides estimating the carbon intensity of electricity production, we propose a new indicator, the carbon intensity of electricity use, which quantifies CO2/kWh of electricity derived useful exergy. Results show final to useful efficiency dilution until World War I (50% to 30%) due to a decrease in share of the high-efficiency transport sector and from mid-1940s to 1960 and mid-1990s onwards (58% to 47% and 47% to 40%) due to an increase in share of the low efficiency commercial and residential sector. Decarbonization from 1900 to mid-1960s, with carbon intensities of electricity production and use dropping respectively from 12.8 to 0.2 and from 33.6 to 0.4 kg CO2/kWh due to an increase in thermoelectricity efficiencies and an increase in share of hydro. Then, a period of carbon deepening until 1990 with carbon intensities tripling due to a shift in shares from hydro to thermoelectricity and more recently a period of decarbonization with carbon intensities decreasing to 0.35 and 0.9 kg CO2/kWh, due to the increase in renewable electricity despite a dilution in final to useful efficiency.

Sign in / Sign up

Export Citation Format

Share Document