O-216 Culture medium used in IVF-treatment impacts post-implantation embryonic growth and developmental trajectories in a sex-specific manner

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
L Van Duijn ◽  
R Steegers-Theunissen ◽  
E Baart ◽  
S Willemsen ◽  
J Laven ◽  
...  
Keyword(s):  
Birth Outcomes ◽  
Growth And Development ◽  
Culture Medium ◽  
Culture Media ◽  
First Trimester ◽  
Fetal Sex ◽  
Crown Rump Length ◽  
Embryonic Growth ◽  
Post Implantation ◽  
Specific Impact

Abstract Study question What is the (sex-specific) impact of two different culture media used in in vitro fertilization (IVF) treatment on post-implantation growth and development? Summary answer Embryos, especially males, cultured in SAGE 1-Step grow and morphologically develop faster in the first trimester, when compared to those cultured in Vitrolife G-1 PLUS. What is known already Increasing success rates after IVF can be attributed to several advancements, such as improved culture conditions. Culture media are of special interest, as they supply the embryo with essential nutrients and have previously been shown to impact birthweight. Moreover, IVF pregnancies are associated with an increased male:female ratio. However, it is unknown if culture media also have an impact prenatally. Therefore, our aim is to study the (sex-specific) impact of two different culture media (SAGE 1-Step and Vitrolife G-1 PLUS) used in IVF treatment on first-trimester embryonic growth and development, and fetal outcomes. Study design, size, duration Women with a viable singleton pregnancy were included before 10 weeks of gestation in the Rotterdam Periconception Cohort, an ongoing prospective tertiary hospital-based study, conducted since November 2009. Participants/materials, setting, methods A total of 879 pregnancies were included; 153 after culture in Vitrolife G-1 PLUS, 251 after culture in SAGE 1-Step and 475 naturally conceived. First-trimester growth and development, defined by serial crown-rump length (CRL), embryonic volume (EV) and Carnegie stages measurements were performed using state-of-the-art imaging techniques. Secondary outcomes included second trimester estimated fetal weight (EFW) and birth outcomes, and were retrieved from medical records. Main results and the role of chance Linear mixed model analyses, adjusted for gestational age and maternal characteristics, showed that embryos cultured in SAGE 1-Step grow faster than those cultured in Vitrolife G-1 PLUS (βEV 0.030 ∛cm3 (95%CI 0.008-0.052), p=0.007). CRL and Carnegie stages were not statistically different between culture media. After stratification for fetal sex, similar results were observed for male embryos (βEV 0.048 ∛cm3 (95%CI 0.015-0.081), p=0.005), but not for female embryos. EFW and birth outcomes were comparable between culture media in the total population and after stratification for fetal sex. Embryos cultured in SAGE 1-Step also grow faster than those conceived naturally (βEV 0.033 ∛cm3 (95%CI 0.006-0.060), p=0.018). This association was also most pronounced in male embryos (βEV 0.066 ∛cm3 (95%CI 0.024-0.108), p=0.002). Limitations, reasons for caution Although this study has a prospective design, its observational character does not exclude residual confounding. Furthermore, the external validity of this explorative study is limited, since participants were recruited from a tertiary university hospital. Wider implications of the findings Culture in SAGE 1-Step culture medium is associated with faster first-trimester growth and development, especially in male embryos. This may be the result of altered susceptibility to preimplantation environmental stressors. Further research should focus on the (sex-specific) impact of culture media on postnatal development and the susceptibility to non-communicable diseases. Trial registration number N/A

scholarly journals Selection of the optimal culture medium for cultivation Fusarium sporotrichioides Sherb

2020 ◽  
Author(s):  
I.L. Astapchuk ◽  
◽  
N.A. Marchenko ◽  
G.V. Yakuba ◽  
A.I. Nasonov ◽  
...  
Keyword(s):  
Growth Rate ◽  
Growth And Development ◽  
Maximum Degree ◽  
Culture Medium ◽  
Culture Media ◽  
High Variability ◽  
Fusarium Sporotrichioides ◽  
Cultural Characteristics ◽  
Colony Diameter ◽  
Selection Of

The influence of various culture media on the growth, morphological and cultural characteristics of the fungus F. sporotrichioides was studied. Ten culture media were used in our research. A comparative study of the growth rate of the F. sporotrichioides mycelium made it possible to identify two media that are the most suitable for the cultivation and identification of this species, namely carrot and tomato agar. We took into account such criteria as ensuring the maximum degree of sporulation, rapid growth and development of mycelium (the 7th day), colony diameter (71–78 mm), as well as the ease of preparation. Nirenberg culture medium can be used to obtain a large number of conidia of the fungus. Because of the high variability of cultural characteristics of F. sporotrichioides, we recommend using different composition of media.

scholarly journals Fetal programming in 2-year-old calving heifers: peri-conception and first trimester protein restriction alters fetal growth in a gender-specific manner

2014 ◽  
Vol 54 (9) ◽  
pp. 1333 ◽  
Author(s):  
K. J. Copping ◽  
A. Hoare ◽  
M. Callaghan ◽  
I. C. McMillen ◽  
R. J. Rodgers ◽  
...  
Keyword(s):  
Fetal Growth ◽  
Fetal Programming ◽  
Growth And Development ◽  
Dietary Intervention ◽  
Maternal Nutrition ◽  
First Trimester ◽  
Protein Restriction ◽  
Production Traits ◽  
Long Term Effects ◽  
Crown Rump Length

Protein restriction in early bovine gestation affects post-natal reproduction and production traits in progeny. This experiment evaluated the effects of dietary protein restriction during the peri-conception period and first trimester in yearling heifers on conceptus growth and development; this period of dietary intervention being earlier than any previous bovine fetal programming studies. Three-hundred and sixty primiparous 12-month-old Santa Gertrudis heifers were individually fed high [14% crude protein (CP)] or low (7% CP) diets for 60 days before conception. At 23 days post-conception (dpc), each high (HPERI) or low (LPERI) group was again split into high (HPOST) or low (LPOST) protein groups yielding four treatment groups in a 2 × 2 factorial design. From the end of the first trimester of gestation (98dpc), the pregnant heifers were individually fed a 12% CP diet until parturition. Forty-six fetuses were excised at 98dpc. Sixty-four heifers went on to calve. Conceptus development was assessed via transrectal ultrasound from 36dpc, fetal necropsy at 98dpc and live calf measures at term. At 36dpc, HPERI diet increased fetal crown–rump length (CRL) (P < 0.05) and at the 60dpc scan, biparietal diameter (BPD) tended to be increased by HPOST diet (P < 0.1) though the greater effect upon BPD was still the HPERI diet (P < 0.05). At 60dpc, BPD in the male fetus was affected by the peri-conception diet (P < 0.05), while in females, BPD was not different among nutritional groups. These ultrasound measures of fetal growth were validated by measures of the excised fetus at 98dpc. Fetal weight was heavier (P < 0.01) in those whose mothers were fed the HPOST diet than their LPOST counterparts. Males fetuses were heavier than female fetuses (P < 0.001). Fetal CRL was increased by HPERI diet (P < 0.05) and tended to be increased by HPOST diet (P < 0.1). Fetal BPD tended to be increased by HPERI diet (P < 0.1). In males, BPD tended to be increased in those fetuses whose mothers were fed HPERI (P < 0.1). For females, maternal nutrition during PERI or POST did not affect BPD at 98dpc (P > 0.1). At term, no dietary effect on birthweight was observed (P > 0.1) and males were not heavier than females (P > 0.1). These results suggest that maternal protein intake during the peri-conception (–60 to 23dpc) and first trimester (24–98dpc) may influence early conceptus growth and development in the bovine. The long-term effects on offspring metabolism and post-natal development of this dietary intervention are yet to be determined.

scholarly journals Sex differences in fetal growth and immediate birth outcomes in a low-risk Caucasian population

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Sander Galjaard ◽  
Lieveke Ameye ◽  
Christoph C. Lees ◽  
Anne Pexsters ◽  
Tom Bourne ◽  
...  
Keyword(s):  
Birth Outcomes ◽  
Fetal Growth ◽  
Head Circumference ◽  
Growth Curves ◽  
First Trimester ◽  
Low Risk ◽  
Abdominal Circumference ◽  
Fetal Ultrasound ◽  
Crown Rump Length ◽  
Gender Specific

Abstract Background According to the WHO Multicentre Growth Reference Study Group recommendations, boys and girls have different growth trajectories after birth. Our aim was to develop gender-specific fetal growth curves in a low-risk population and to compare immediate birth outcomes. Methods First, second, and third trimester fetal ultrasound examinations were conducted between 2002 and 2012. The data was selected using the following criteria: routine examinations in uncomplicated singleton pregnancies, Caucasian ethnicity, and confirmation of gestational age by a crown-rump length (CRL) measurement in the first trimester. Generalized Additive Model for Location, Scale and Shape (GAMLSS) was used to align the time frames of the longitudinal fetal measurements, corresponding with the methods of the postnatal growth curves of the WHO MGRS Group. Results A total of 27,680 complete scans were selected from the astraia© ultrasound database representing 12,368 pregnancies. Gender-specific fetal growth curves for biparietal diameter (BPD), head circumference (HC), abdominal circumference (AC), and femur length (FL) were derived. The HC and BPD were significantly larger in boys compared to girls from 20 weeks of gestation onwards (p < 0.001) equating to a 3-day difference at 20–24 weeks. Boys were significantly heavier, longer, and had greater head circumference than girls (p < 0.001) at birth. The Apgar score at 1 min (p = 0.01) and arterial cord pH (p < 0.001) were lower in boys. Conclusions These longitudinal fetal growth curves for the first time allow integration with neonatal and pediatric WHO gender-specific growth curves. Boys exceed head growth halfway of the pregnancy, and immediate birth outcomes are worse in boys than girls. Gender difference in intrauterine growth is sufficiently distinct to have a clinically important effect on fetal weight estimation but also on the second trimester dating. Therefore, these differences might already play a role in early fetal or immediate neonatal management.

scholarly journals The Impact of Neighbourhood Deprivation on Embryonic Growth Trajectories: Rotterdam Periconception Cohort

2019 ◽  
Vol 8 (11) ◽  
pp. 1913
Author(s):  
Dionne V. Gootjes ◽  
Maria P. H. Koster ◽  
Sten P. Willemsen ◽  
Anton H. J. Koning ◽  
Eric A. P. Steegers ◽  
...  
Keyword(s):  
Vegetable Intake ◽  
Geographic Origin ◽  
First Trimester ◽  
Crown Rump Length ◽  
Neighbourhood Deprivation ◽  
Supplement Use ◽  
Embryonic Growth ◽  
Deprived Neighbourhoods ◽  
Adverse Pregnancy ◽  
The Impact

Background: Neighbourhood deprivation is a risk factor for impaired health and adverse pregnancy outcomes. We investigated whether living in a deprived neighbourhood is associated with embryonic growth. Methods: From the Predict cohort, we studied 566 women who underwent repeated first trimester ultrasound examinations. Crown rump length (CRL; n = 1707) and embryonic volume (EV; n = 1462) were measured using three-dimensional techniques. Neighbourhood deprivation was assessed using the neighbourhood status scores (NSS) of the Dutch Social Cultural Planning office. A high NSS represents a non-deprived neighbourhood. Associations between the NSS and embryonic growth were investigated using linear mixed models. Adjustment was performed for individual-level factors: maternal age, geographic origin, educational level, BMI, folic acid supplement use, fruit and vegetable intake, alcohol use and smoking habits. Results: The NSS was negatively associated with embryonic growth: a higher score (a less deprived neighbourhood) was associated with a smaller CRL and EV; adjusted β: −0.025 (95% CI −0.046, −0.003) and adjusted β: −0.015 (95% CI −0.026, −0.003). At 11 weeks of pregnancy, we observed a 0.55 cm3 smaller EV (7.65 cm3 vs. 7.10 cm3) and 1.08 mm smaller CRL (43.14 mm vs. 42.06 mm) in the highest compared to the lowest category. Conclusion: In deprived neighbourhoods, embryos are larger than in non-deprived neighbourhoods.

scholarly journals Effects of Different Paclobutrazol-Cultar Concentrations on the Micropropagation of Sugarcane (Saccharum Officinarum) Variety CPCL99-4455

2018 ◽  
Vol 7 ◽  
pp. 1011-1018 ◽  
Author(s):  
Dion Daniels ◽  
Nidia Panti ◽  
David Guerra ◽  
Stephen Williams
Keyword(s):  
Growth And Development ◽  
Culture Medium ◽  
Culture Media ◽  
Saccharum Officinarum ◽  
Average Height ◽  
Ex Vitro ◽  
Agricultural Exports ◽  
Multiplication Coefficient ◽  
Important Cash Crop

Sugarcane (Saccharum officinarum), being an important cash crop in Belize, accounts for 60% of agricultural exports providing employment for thousands of Belizeans. This research was carried out to determine the effects of different Paclobutrazol (PBZ-Cultar) concentrations used in the culture media on in vitro multiplication of sugarcane variety CPCL99-4455. Three PBZ-Cultar concentrations were tested and compared with the control. The parameters evaluated to determine the effects of PBZ-Cultar were average height, number of dead leaves and multiplication coefficient. The plants from this experiment were planted in the acclimatization phase to determine if the use of PBZ-Cultar had any effect of the growth and development of the plants ex vitro. The results revealed that the culture medium supplemented with 0.08% PBZ-Cultar concentration had the best results both in the multiplication phase as well as the acclimatization phase.

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

scholarly journals Untargeted Metabolomics Approach for the Discovery of Environment-Related Pyran-2-ones Chemodiversity in a Marine-Sourced Penicillium restrictum

Marine Drugs ◽  
2021 ◽  
Vol 19 (7) ◽  
pp. 378
Author(s):  
Van-Tuyen Le ◽  
Samuel Bertrand ◽  
Thibaut Robiou du Pont ◽  
Fabrice Fleury ◽  
Nathalie Caroff ◽  
...  
Keyword(s):  
Culture Medium ◽  
Blue Mussel ◽  
Culture Media ◽  
Principal Component ◽  
Chemical Diversity ◽  
Untargeted Metabolomics ◽  
High Chemical ◽  
Medium Mass ◽  
Metabolomics Study ◽  
Penicillium Restrictum

Very little is known about chemical interactions between fungi and their mollusc host within marine environments. Here, we investigated the metabolome of a Penicillium restrictum MMS417 strain isolated from the blue mussel Mytilus edulis collected on the Loire estuary, France. Following the OSMAC approach with the use of 14 culture media, the effect of salinity and of a mussel-derived medium on the metabolic expression were analysed using HPLC-UV/DAD-HRMS/MS. An untargeted metabolomics study was performed using principal component analysis (PCA), orthogonal projection to latent structure discriminant analysis (O-PLSDA) and molecular networking (MN). It highlighted some compounds belonging to sterols, macrolides and pyran-2-ones, which were specifically induced in marine conditions. In particular, a high chemical diversity of pyran-2-ones was found to be related to the presence of mussel extract in the culture medium. Mass spectrometry (MS)- and UV-guided purification resulted in the isolation of five new natural fungal pyran-2-one derivatives—5,6-dihydro-6S-hydroxymethyl-4-methoxy-2H-pyran-2-one (1), (6S, 1’R, 2’S)-LL-P880β (3), 5,6-dihydro-4-methoxy-6S-(1’S, 2’S-dihydroxy pent-3’(E)-enyl)-2H-pyran-2-one (4), 4-methoxy-6-(1’R, 2’S-dihydroxy pent-3’(E)-enyl)-2H-pyran-2-one (6) and 4-methoxy-2H-pyran-2-one (7)—together with the known (6S, 1’S, 2’S)-LL-P880β (2), (1’R, 2’S)-LL-P880γ (5), 5,6-dihydro-4-methoxy-2H-pyran-2-one (8), (6S, 1’S, 2’R)-LL-P880β (9), (6S, 1’S)-pestalotin (10), 1’R-dehydropestalotin (11) and 6-pentyl-4-methoxy-2H-pyran-2-one (12) from the mussel-derived culture medium extract. The structures of 1-12 were determined by 1D- and 2D-MMR experiments as well as high-resolution tandem MS, ECD and DP4 calculations. Some of these compounds were evaluated for their cytotoxic, antibacterial, antileishmanial and in-silico PTP1B inhibitory activities. These results illustrate the utility in using host-derived media for the discovery of new natural products.

Crown–rump length measurement: a new age for first‐trimester ultrasound?

2021 ◽  
Author(s):  
J. Stirnemann ◽  
M. Massoud ◽  
N. Fries ◽  
C. Dumont ◽  
G. Haddad ◽  
...  
Keyword(s):  
First Trimester ◽  
New Age ◽  
Length Measurement ◽  
Crown Rump Length ◽  
First Trimester Ultrasound
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