367Association between dietary fatty acid intake, serum levels of fatty acids and ABCA1 DNA methylation

Abstract Background Although previous studies have demonstrated that n-3 polyunsaturated fatty acids (PUFA) are inversely associated with a risk of CVD through gene expression, recent evidence suggests that it may arise from changes in DNA methylation. Therefore, we investigated whether dietary FA intake and serum FA levels are associated with ATP-binding cassette transporter A1 (ABCA1), a gene associated with HDL-cholesterol metabolism. Methods A total of 298 subjects (137 men, mean age of 63.2) without clinical history and medication participated in this cross-sectional study. We used the pyrosequencing method to measure DNA methylation levels at 8 CpG sites within ABCA1 gene and calculated mean DNA methylation level. Dietary FA intake were assessed with the food frequency questionnaire. Results We found that ABCA1 DNA methylation levels were significantly lower with higher dietary intake of n-3PUFA (β = –5.75, p = 0.001), which is observed both in men (β = –4.85, p = 0.001) and women (β = –7.24, p = 0.007). In addition, ABCA1 DNA methylation levels were significantly lower with higher serum levels of n-3PUFA in men (β = –2.78, p = 0.05), but not in women (β = 1.61, p = 0.31). Conclusions The results suggest that higher dietary n-3 PUFA intake and serum levels of n-3 PUFA are associated with lower ABCA1 DNA methylation in a Japanese population. Key messages Higher dietary n-3 PUFA intake can be a prevention from dyslipidemia and CVD via low level of ABCA1 DNA methylation

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Caveolin-1 Genetic Polymorphism Interact with Polyunsaturated Fatty Acids to Modulate Metabolic Syndrome Risk

British Journal Of Nutrition ◽

10.1017/s0007114521002221 ◽

2021 ◽

pp. 1-23

Author(s):

Faezeh Abaj ◽

Khadijeh Mirzaei

Keyword(s):

Metabolic Syndrome ◽

Fatty Acids ◽

Dietary Intake ◽

Hdl Cholesterol ◽

Serum Levels ◽

Cross Sectional Study ◽

Cholesterol Concentration ◽

Cross Sectional ◽

Caveolin 1 ◽

Pcr Rflp

Abstract Several studies have reported a significant association between metabolic syndrome (MetS) and mortality around the world. Caveolin-1 (CAV-1) has been widely studied in dyslipidemia, and several studies have indicated that CAV-1 genetic variations may correlate with dietary intake of fatty acids. This study aimed to investigate the interaction of CAV-1 rs3807992 with types of dietary fatty acid in MetS risk. This cross-sectional study was carried out on 404 overweight and obese females. Dietary intake was obtained from a 147-item FFQ. The CAV-1 genotype was measured using the PCR-RFLP method. Anthropometric values and serum levels (TC, LDL, HDL, TG, FBS) were measured by standard methods. It was observed that the (AA+AG) group had significantly higher BMI, WC, and DBP (P=0.02, P=0.02, and P=0.01, respectively) and lower serum LDL, HDL, and TC (P < 0.05) than the GG group. It was found that A allele carriers were at higher odds of MetS (P= 0.01), abdominal obesity (P=0.06), increased TG concentration (P=0.01), elevated blood pressure (BP) (P=0.01), increased glucose concentration (P=0.45), and decreased HDL-cholesterol concentration (P=0.03). Moreover, the interaction of CAV-1 and SFA intake was significant in terms of MetS (P=0.03), LDL (P=0.03), and BP (P=0.01). Additionally, the (AA+AG) group was significantly related to PUFA intake in terms of MetS (P=0.04), TG (P=0.02), glucose (P=0.02), and HOMA-IR (P= 0.01). Higher PUFA consumption might attenuate the CAV-1 rs3807992 associations with MetS, and individuals with greater genetic predisposition appeared to have a higher risk of MetS, associated with higher SFA consumption.

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Galectin-3, a marker of cardiac remodeling, is inversely related to serum levels of marine omega-3 fatty acids. A cross-sectional study

JRSM Cardiovascular Disease ◽

10.1177/2048004017729984 ◽

2017 ◽

Vol 6 ◽

pp. 204800401772998 ◽

Cited By ~ 2

Author(s):

K Laake ◽

I Seljeflot ◽

EB Schmidt ◽

P Myhre ◽

A Tveit ◽

...

Keyword(s):

Fatty Acids ◽

Cardiac Remodeling ◽

Serum Levels ◽

Cross Sectional Study ◽

Omega 3 Fatty Acids ◽

Omega 3 ◽

Sectional Study ◽

Cross Sectional ◽

Galectin 3

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Associations of Serum Fatty Acids with Serum Lipids Parameters in Ischaemic Stroke Patients

10.21203/rs.2.10384/v1 ◽

2019 ◽

Author(s):

Takahisa Mori ◽

Kazuhiro Yoshioka

Keyword(s):

Fatty Acids ◽

Ischaemic Stroke ◽

Serum Lipids ◽

Linear Regression Analysis ◽

Hdl Cholesterol ◽

Serum Levels ◽

Stroke Patients ◽

Cross Sectional ◽

Positive Factor ◽

Serum Fatty Acids

Abstract Background: Serum fatty acids (s-FA) are likely associated with serum lipids parameters (s-LP), such as total cholesterol (T-CHO), LDL-cholesterol (LDL-C), HDL-cholesterol (HDL-C) and triglycerides (TG), because fatty acids (FA) are components of lipoproteins. However, little is known about the association of s-FA with s-LP. The aim of our retrospective study was to investigate the association of s-FA with s-LP in Ischaemic stroke patients. Methods: We conducted a cross-sectional study of ischaemic stroke patients aged 50 years or older who 1) were admitted to our institution between September 2015 and March 2017 within 24 hours of stroke onset and 2) took blood examination of s-FA levels of palmitic acid (PA), stearic acid (StA), oleic acid (OlA), linoleic acid (LiA), arachidonic acid (AA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). We evaluated correlation of serum levels or composition percentage (%) of FA with s-LP. Results: One hundred ninety-one patients met our inclusive criteria. Their average age was 74.4 years, BMI was 23.4 kg/m2, T-CHO was 203.4, LDL-C was 121.7, HDL-C was 58.5 and TG was 116.2 mg/dl. Stepwise linear regression analysis showed that LiA and AA were independent positive factors of T-CHO, AA and LiA% were independent positive factors of LDL-C, AA was an independent positive factor of HDL-C, AA% and LiA% were independent inverse factors of HDL-C, and OlA was an independent positive factor of TG. Conclusions: Serum FA had correlation with s-LP in ischaemic stroke patients. The results warrant clinical application of s-FA modification to improve s-LP.

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Peripheral polyneuropathy in severely obese patients with metabolic syndrome but without diabetes: Association with low HDL-cholesterol

Revista da Associação Médica Brasileira ◽

10.1590/1806-9282.63.04.324 ◽

2017 ◽

Vol 63 (4) ◽

pp. 324-331 ◽

Cited By ~ 4

Author(s):

Otto Henrique Nienov ◽

Luciana Matte ◽

Lisiane Stefani Dias ◽

Helena Schmid

Keyword(s):

Metabolic Syndrome ◽

Univariate Analysis ◽

Hdl Cholesterol ◽

Serum Levels ◽

Cross Sectional Study ◽

Serum Triglycerides ◽

Cross Sectional ◽

Peripheral Polyneuropathy ◽

Low Hdl ◽

Severely Obese

Summary Introduction: The purpose of this study was to evaluate the prevalence of peripheral polyneuropathy (PPN) in subjects with grade II and III obesity (Ob-II,III) and metabolic syndrome (MetS) but without diabetes and to investigate possible associated factors. Method: A cross-sectional study was performed in non-diabetic Ob-II,III,MetS patients using the Michigan Neuropathy Screening Instrument (MNSI) to assess the presence of PPN. Results: A total of 24 of 218 non-diabetic Ob-II,III,MetS patients had PPN. Based on univariate analysis, serum levels of LDL-cholesterol (p=0.046) were significantly associated with PPN, while serum triglycerides (p=0.118) and low HDL-cholesterol (p=0.057) showed a tendency toward this association. On a Poisson regression analysis, when the three possible associations were included, low HDL-cholesterol (p=0.047) remained independently associated. Conclusion: In non-diabetic Ob-II,III,MetS patients, PPN defined by the MNSI showed a high prevalence and was associated with low levels of HDL-cholesterol. In order to diagnose that complication, neurological evaluation should be performed in these patients.

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PRE- AND POSTOPERATIVE IN BARIATRIC SURGERY: SOME BIOCHEMICAL CHANGES

ABCD Arquivos Brasileiros de Cirurgia Digestiva (São Paulo) ◽

10.1590/0102-6720201600s10017 ◽

2016 ◽

Vol 29 (suppl 1) ◽

pp. 67-71 ◽

Cited By ~ 2

Author(s):

Amanda Kaseker TEDESCO ◽

Rafaela BIAZOTTO ◽

Telma Souza e Silva GEBARA ◽

Maria Paula Carlini CAMBI ◽

Giorgio Alfredo Pedroso BARETTA

Keyword(s):

Bariatric Surgery ◽

Vitamin B12 ◽

Ldl Cholesterol ◽

Hdl Cholesterol ◽

Serum Levels ◽

Cross Sectional Study ◽

Surgical Patients ◽

Nutritional Deficiencies ◽

Cross Sectional ◽

Significant Difference

ABSTRACT Background: The bariatric surgery may cause some nutritional deficiencies. Aim: To compare the serum levels of biochemical markers, in iimmediate post-surgical patients who were submitted to bariatric surgery. Methods: Non-concurrent prospective cross-sectional study. The analysis investigated data in medical charts of pre-surgical and immediate post-surgical patients who were submitted to bariatric surgery, focusing total cholesterol, HDL cholesterol, LDL cholesterol, triglycerides, C reactive protein, vitamin B12 levels, folic acid, homocysteine values, iron and serum calcium at the referred period. Results: Twenty-nine patients of both genders were evaluated. It was observed weight loss from 108.53 kg to 78.69 kg after the procedure. The variable LDL-c had a significant difference, decreasing approximately 30.3 mg/dl after the surgery. The vitamin B12 serum average levels went from 341.9 pg/ml to 667.2 pg/ml. The triglycerides values were in a range of 129.6 mg/dl-173.3 mg/dl, and 81.9 mg/dl-105.3 mg/dl at the pre- and postoperative respectively. CRP levels fall demonstrated reduction of inflammatory activity. The variable homocysteine was tested in a paired manner and it did not show a significant changing before or after, although it showed a strong correlation with LDL cholesterol. Conclusion: Eligible patients to bariatric surgery frequently present pre-nutritional deficiencies, having increased post-surgical risks when they don´t follow an appropriate nutritional follow-up.

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Ultrasound as a method to evaluate the distribution of abdominal fat in obese prepubertal children and the relationship between abdominal fat and metabolic alterations

Radiologia Brasileira ◽

10.1590/0100-3984.2016.0230 ◽

2018 ◽

Vol 51 (5) ◽

pp. 293-296 ◽

Cited By ~ 3

Author(s):

Arine Santos Peçanha ◽

Alexandra Maria Monteiro ◽

Fernanda Mussi Gazolla ◽

Isabel Rey Madeira ◽

Maria Alice Neves Bordallo ◽

...

Keyword(s):

Hdl Cholesterol ◽

Serum Levels ◽

Cross Sectional Study ◽

Abdominal Fat ◽

Normal Weight ◽

Model Assessment ◽

Obese Children ◽

Cross Sectional ◽

Prepubertal Children ◽

Metabolic Alterations

Abstract Objective: To evaluate, using ultrasound, the distribution of abdominal fat in obese prepubertal children, as well as its possible correlation with metabolic changes due to obesity. Materials and Methods: This was a cross-sectional study of prepubescent children: 77 obese children (33 girls and 44 boys), with a mean age of 7.31 years; and 31 normal-weight children (17 girls and 14 boys), with a mean age of 7.32 years. In all of the children, abdominal wall thickness (AWT) and abdominal fat thickness (AFT) were measured by ultrasound. For the evaluation of the associated metabolic alterations, serum levels of glycemia, HDL cholesterol, triglycerides, and insulin were determined. Results: The obese children presented with greater abdominal fat, predominantly greater AWT, without a significant gender-related difference in AWT or AFT. The homeostasis model assessment of insulin resistance (HOMA-IR) showed a significant direct correlation with AWT and AFT. Conclusion: In obese prepubertal children, the AWT, as measured by ultrasound, was shown to be more closely related to the HOMA-IR than to the lipid metabolism or glycemia.

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Promoter Methylation of LEP and LEPR before and after Bariatric Surgery: A Cross-Sectional Study

Obesity Facts ◽

10.1159/000511918 ◽

2021 ◽

pp. 1-7

Author(s):

Julia Wilhelm ◽

Anna Birkenstock ◽

Vanessa Buchholz ◽

Astrid Müller ◽

Sherif Adel Aly ◽

...

Keyword(s):

Bariatric Surgery ◽

Dna Methylation ◽

Promoter Methylation ◽

Leptin Receptor ◽

Receptor Gene ◽

Serum Levels ◽

Cross Sectional Study ◽

Feedback Mechanism ◽

Epigenetic Mechanism ◽

Cross Sectional

Introduction: DNA methylation constitutes one important epigenetic mechanism that regulates gene expression in human cells. With regard to obesity, bariatric surgery-induced weight loss has been associated with promoter methylation changes in several genes. Hyperleptinemia is a characteristic feature of obesity. The underlying regulating mechanisms have not yet been completely elucidated. Methods: We investigated the methylation of the promoters of the leptin gene (LEP) and the leptin receptor gene (LEPR) as well as leptin expression in pre- and postbariatric surgery patients using a comparative cross-sectional design. Results: Our results revealed significantly higher LEP promoter methylation patterns in prebariatric surgery patients compared to postoperatively. DNA methylation of the LEPR promoter was significantly higher in the postoperative group. Moreover, we found significantly higher leptin serum levels in patients before the bariatric surgery than afterwards. Discussion: These findings strengthen the suggestion that there is an association between LEP expression and LEP methylation in obesity. We suggest that the epigenetic profile of LEP might be influenced by leptin serum levels in the form of a regulating feedback mechanism.

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Caveolin-1 Genetic Polymorphisms Interact with Fatty Acid Types to Modulate Metabolic Syndrome Risk

10.21203/rs.3.rs-119698/v1 ◽

2020 ◽

Author(s):

Faezeh Abaj ◽

Khadijeh Mirzaei

Keyword(s):

Metabolic Syndrome ◽

Fatty Acids ◽

Fatty Acid ◽

Dietary Intake ◽

Hdl Cholesterol ◽

Serum Levels ◽

Cholesterol Concentration ◽

Human Society ◽

Cross Sectional ◽

Caveolin 1

Abstract Background: Metabolic syndrome (MetS) is related with all-cause mortality. Caveolin-1 (Cav-1) has been widely studied in dyslipidemia, and several studies have indicated that Cav-1 genetic variations may correlate with dietary intake of fatty acids. The aim of the current study was therefore to evaluate the interaction of Cav-1 rs3807992 with types of dietary fatty acid in MetS risk factor status Methods: This cross-sectional study was carried out on 404 overweight and obese females. Dietary intake was obtained from a 147-item FFQ. The CAV-1 genotype was measured using the PCR-RFLP method. Anthropometric values and serum levels (TC, LDL, HDL, TG, FBS) were measured by standard methods. Results: It was observed that the (AA+AG) group had significantly higher BMI, WC and DBP (P=0.02, P=0.02 and P=0.01, respectively) and lower serum LDL, HDL and TC (P < 0.05) than the GG group. It was found that A allele carriers were at higher odds of MetS (P= 0.01), abdominal obesity (P=0.06), increased TG concentration (P=0.01), elevated blood pressure (BP) (P=0.01), increased glucose concentration (P=0.45), and decreased HDL-cholesterol concentration (P=0.03). Moreover, the interaction of Cav-1 and SFA intake was significant in terms of MetS (P=0.01), LDL (P=0.03), DBP (P=0.01) and LDL/HDL (P=0.05). Additionally, the (AA+AG) group was significantly related to PUFA intake in terms of MetS (P=0.04), TG (P=0.02), glucose (P=0.02) and HOMA-IR (P= 0.01). Conclusions: Higher PUFA consumption might attenuate the Cav-1 rs3807992 associations with MetS, and individuals with greater genetic predisposition appeared to have a higher risk of MetS, associated with higher SFA consumption To date, studies on this polymorphism have been animal studies and have not been performed on healthy and obese human society For the first time , this study provides information on the interaction of different fatty acids with the Caveolin gene, which is functionally effective in lipid metabolism

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