PSVI-6 Effect of reduced dietary protein and amino acid supply imposed in the grower and finisher period or solely in the finisher period on growth traits and carcass protein deposition efficiency in pigs

Abstract Above 65 kg empty BW, protein deposition is greater in pigs fed protein-restricted compared to standard diets. It is unclear whether the greater CP deposition rate in the finisher period results from the restricted CP supply in the grower period. To elucidate this question, a growth performance study (22.1-110.0 kg BW) was performed with 48 Swiss Large White pigs. They were assigned to either treatment C, LPF or LPGF. The grower-finisher diets of treatment C were formulated based on the Swiss feeding recommendations whereas those of treatment LPGF contained 20% lower digestible CP and essential amino acid levels than diets C. Pigs of the LPGF treatment were offered in the grower and finisher period the standard and the low CP diets, respectively. The pigs were reared in 1 large pen, equipped with 6 automatic feeders, which allowed measuring the individual ad-libitum feed intake per visit. The BW was determined weekly and carcass composition at slaughter was determined by dual-X-ray-absorpiometry. Growth performance and dual-X-ray-absorpiometry data were analyzed with a linear mixed model using treatment as fixed and litter, litter size and farrowing series as random effects. Despite total CP intake was 6 and 16% lower (P< 0.05) in the LPGF than the LPF and C groups, respectively, overall ADG among treatments did not differ. In accordance, gain-to-CP-intake ratio was 9 and 20% greater (P< 0.05) in the LPGF than the LPF and C group. The carcass protein content did not differ whereas carcass fat content was 9% greater (P< 0.05) in LPGF than LPF and C pigs. Carcass protein deposition efficiency was 0.5 and 6.7%-units greater (P< 0.05) in LPGF than LPF and C pigs, respectively. In conclusion, lowering the CP supply by 20% in the grower and finisher compared to solely the finisher period or not at all has no detrimental effect on growth performance, markedly improves CP utilization for carcass protein deposition but slightly increases carcass fatness.

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PSIX-21 Effect of reduced digestible protein and amino acid supply imposed in the grower and finisher period or solely in the finisher period on growth performance and feeding behaviour

Journal of Animal Science ◽

10.1093/jas/skz258.694 ◽

2019 ◽

Vol 97 (Supplement_3) ◽

pp. 348-349

Author(s):

Giuseppe Bee ◽

Guy Maikoff

Keyword(s):

Growth Performance ◽

Mixed Model ◽

Linear Mixed Model ◽

Essential Amino Acids ◽

Performance Study ◽

Large White ◽

Digestible Protein ◽

The Individual ◽

Dietary Treatments ◽

Behaviour Data

Abstract Above 65 kg empty BW, protein deposition is greater in pigs fed protein-restricted compared to standard diets. It is unclear whether the greater CP deposition rate in the finisher period results from the restricted CP supply in the grower phase. To elucidate this, a growth performance study (22.1–110.0 kg BW) was performed with 48 Large-White pigs. They were assigned within litter to 3 dietary treatments: C, LP-F, LP-GF. The grower-finisher diets of treatment C were formulated based on the Swiss feeding recommendations whereas those for the LP-GF treatment contained 20% lower digestible CP and essential amino acids levels than the C diets. Pigs of the LP-GF group were offered in the grower and finisher period the standard and the low CP diets, respectively. The pigs were reared in one large pen, equipped with 6 automatic feeders, which allowed measuring the individual ad-libitum feed intake per visit. The BW was determined weekly. Growth performance and feed behaviour data were analysed with a linear mixed model using treatment as fixed and litter, litter size and farrowing series as random effects. Despite total CP intake was 6 and 16% lower (P < 0.05) in the LP-GF than the LP-F and C groups, respectively, overall ADG from 22.1 to 110.0 kg BW did not differ. Accordingly, gain-to-CP-intake ratio was 9 and 20% greater (P < 0.05) in the LP-GF than the LP-F and C group. In the grower, but not the finisher period, LP-GF pigs visited 1.6-times more (P < 0.05) the feeder and ingested 12% less (P < 0.05) feed per visit than C and LP-F pigs. These data suggest that lowering the CP supply by 20% in the grower and finisher compared to solely the finisher period or not at all has no detrimental effect on growth performance and markedly improves CP utilization for growth.

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Impact of 100% organic diets on pig performance, carcass composition and carcass nutrient deposition efficiency

Organic Agriculture ◽

10.1007/s13165-021-00348-0 ◽

2021 ◽

Author(s):

Giuseppe Bee ◽

Silvia Ampuero Kragten ◽

Barbara Früh ◽

Marion Girard

Keyword(s):

Growth Traits ◽

Press Cake ◽

Deposition Efficiency ◽

Essential Amino Acids ◽

Carcass Composition ◽

Fibre Content ◽

Current Standard ◽

Crude Fibre ◽

Protein Deposition ◽

Crude Fibre Content

AbstractThe ultimate objective of organic pig production is to use ingredients of 100% organic origin in the animals’ diets. However, this approach is challenging, especially when needing to ensure adequate quantities of essential amino acids (EAAs) and protein in grower-finisher diets. The current standard is 95% organic feed ingredients, but this percentage can be increased by reducing the EAA supply or by accepting an unbalanced EAA profile. A further incentive also exists to include forages in organic diets as a way to raise dietary crude fibre content. The current study therefore investigates three organic diets (T95: 5% of non-organic origin; T100: 0% of non-organic origin and T100-CF: T100 plus an increased crude fibre level), which were offered ad libitum to 48 pigs (16 per diet) weighing 22 to 110 kg. Comparison of the three diets revealed that increasing the crude fibre content (T100-CF) reduced growth, increased total feed intake and impaired feed and protein deposition efficiency, but the unbalanced EAA profile (T100) had no effect on growth traits and protein deposition efficiency. The T100 and T100-CF pigs differed from the T95 pigs in terms of feeding behaviour traits, as they displayed more frequent but shorter feeder visits, shorter between-meal intervals and smaller meals. Increasing the fibre content with press cake ingredients raised the degree of unsaturation of the backfat and might cause problems in meat processing. In conclusion, the results revealed that pigs could cope with a mild EAA imbalance.

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249 Increasing Structural Fiber Improves Growth Performance of Nursery Pigs

Journal of Animal Science ◽

10.1093/jas/skab054.149 ◽

2021 ◽

Vol 99 (Supplement_1) ◽

pp. 93-93

Author(s):

Sara Ebarb ◽

Sabrina May ◽

Mark D Newcomb

Keyword(s):

Growth Performance ◽

Mixed Model ◽

Linear Mixed Model ◽

Two Phase ◽

Nursery Pigs ◽

High Fiber ◽

Rice Hulls ◽

Fiber Treatment ◽

Wheat Middlings

Abstract Fiber ingredients in swine diets have various components that affect the intestinal tract distinctively. The objective of this study was to determine the effect of structural fiber sources on growth performance of nursery pigs. A total of 1,760 weanling pigs (initial BW = 6.12 ± 0.3 kg, 16 reps/trt, 22 pigs/pen) were used in a study with 5 dietary treatments: 1) Complex nursery diet with no additional fiber (CON); 2) CON + 2% rice hulls; 3) CON + 4% rice hulls; 4) CON + 6% wheat middlings; 5) CON + 12% wheat middlings. Fiber ingredients were added at the expense of corn and some processed soybean meal to maintain similar dietary protein levels. Using an in vitro fermentation estimation of fermentation for rice hulls and wheat middlings, diets were constructed to add similar levels of structural (non-fermentable NDF) fiber between low and high additions of rice hulls and wheat middlings. Pens across two barns were randomly allotted independent of one another and within a location block to one of the five treatments on d 0 of a two-phase study (d 0–11 and d 11–20.5 post-weaning). Data were analyzed by general linear model in R. Mortality and removal data were analyzed as a generalized linear mixed model with a binomial distribution. Contrasts tested the effect of additional fiber (CON vs treatments 2–5), effect of medium vs high fiber (treatment 2/4 vs 3/5), effect of source of fiber (treatment 2/3 vs 4/5), and the interaction of level and source of fiber. Overall (Table 1), additional fiber resulted in increased ADFI (P < 0.05) and tended to increase ADG (P < 0.10). Probability of mortality and removal was reduced (P < 0.05) when additional fiber was included. In summary, increasing the level of structural fiber improved performance and livability of nursery pigs.

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128 Effects of a Bacillus-based probiotic and prebiotics on nursery pig performance, fecal consistency, and fecal microflora

Journal of Animal Science ◽

10.1093/jas/skz122.139 ◽

2019 ◽

Vol 97 (Supplement_2) ◽

pp. 75-76

Author(s):

Mariana Boscato Menegat ◽

Michaela B Braun ◽

Joel M DeRouchey ◽

Jason C Woodworth ◽

Jim Bryte ◽

...

Keyword(s):

Bacillus Subtilis ◽

Growth Performance ◽

Mixed Model ◽

Linear Mixed Model ◽

Experimental Unit ◽

Bacillus Sp ◽

Nursery Pigs ◽

Fecal Microflora ◽

Microbial Analysis ◽

Diet Control

Abstract This study evaluated the progeny of sows fed a control or probiotic diet with Bacillus subtilis C-3102 (Calsporin®, Calpis Co. Ltd., Tokyo, Japan) at 500,000 and 1,000,000 CFU/g diet in gestation and lactation, respectively. A total of 358 weaned pigs (DNA 241 × 600) were used in a 42-d trial with 4–5 pigs/pen and 18–19 pens/treatment. Pens were allotted to treatments in a completely randomized design based on BW at weaning. Treatments were a 2×2 factorial with main effects of sow diet (control vs. probiotic) and nursery diet (control vs. probiotic). In the nursery probiotic diet, a product based on probiotic Bacillus subtilis C-3102 at 500,000 CFU/g diet and prebiotics β-glucans and mannan oligosaccharides was included at 0.05% (BacPack ABF™, Quality Technology International, Inc., Elgin, IL). Diets were corn-soybean meal-based. Growth performance and fecal consistency on a 1-to-5 scale were evaluated weekly. Fecal samples were collected for microbial analysis by culture method and bacterial quantification of Bacillus subtilis C-3102, total Bacillus sp., Lactobacillus sp., Clostridium perfringens, Salmonella spp., Enterococcus sp., Enterobacteriaceae, total aerobes, and total anaerobes. Data were analyzed using a linear mixed model (PROC GLIMMIX, SAS®) with pen as experimental unit. There was no evidence for effect of sow diet, nursery diet, or interactions (P > 0.10) on overall nursery growth performance and fecal consistency. However, growth performance from d 21 to 42 and final BW were greater (P < 0.05) in progeny of control-fed sows compared to probiotic-fed sows. Microbial analysis revealed an increase (P < 0.01) in Bacillus subtilis C-3102 and, consequently, total Bacillus sp. in fecal microflora of probiotic-fed pigs. In conclusion, probiotic inclusion to sow diets lowered growth performance of the progeny in late nursery. The probiotic diet provided to sows or nursery pigs did not influence fecal consistency, but altered the fecal microbial population in nursery pigs by increasing total Bacillus sp.

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PSVII-9 Effects of a multi-species direct-fed microbial on growth performance and carcass characteristics of grow-finish pigs

Journal of Animal Science ◽

10.1093/jas/skz122.380 ◽

2019 ◽

Vol 97 (Supplement_2) ◽

pp. 216-216

Author(s):

Mariana Boscato Menegat ◽

Joel M DeRouchey ◽

Jason C Woodworth ◽

Mike D Tokach ◽

Steve S Dritz ◽

...

Keyword(s):

Growth Performance ◽

Mixed Model ◽

Linear Mixed Model ◽

Block Design ◽

Control Diet ◽

Random Effect ◽

Carcass Characteristics ◽

Experimental Unit ◽

Feeding Duration ◽

Corn Distillers

Abstract This study was conducted to determine the effects of a multi-species direct-fed microbial (DFM) product based on lactic acid bacteria and Bacillus subtilis on growth performance and carcass characteristics of grow-finish pigs. A total of 1,188 pigs (PIC 359 × 1050; initially 25.8 kg BW) were used in a 121-d growth trial with 27 pigs/pen and 22 pens/treatment. Pigs were allotted to treatments based on initial BW in a randomized complete block design. Treatments included a control diet and the control diet with added DFM (BiOWiSH Technologies Inc., Cincinnati, OH) included at 0.055% of the diet at the expense of corn. Diets were based on corn, distillers dried grains with solubles, and soybean meal and fed in four dietary phases. Data were analyzed using a linear mixed model (PROC GLIMMIX, SAS®) with treatment as fixed effect, block as random effect, and pen as experimental unit. Overall (d 0 to 121), pigs fed the control diet had greater ADG (P < 0.05) and final BW (P < 0.001) compared to pigs fed the DFM diet (Table 1). There was no evidence for differences (P > 0.05) in ADFI or G:F between treatments. The difference in final BW resulted in heavier (P < 0.05) HCW in control pigs compared to DFM pigs, but no evidence for differences (P > 0.05) was observed in carcass yield, backfat, loin depth, and percentage lean between treatments. In conclusion, the inclusion of this multi-species DFM in growing-finishing diets reduced ADG in this commercial study. This response could be related to inclusion rate, feeding duration, or other factors not identified in this study, warranting further research to characterize the effects on pig performance.

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Genetic architecture and major genes for backfat thickness in pig lines of diverse genetic backgrounds

Genetics Selection Evolution ◽

10.1186/s12711-021-00671-w ◽

2021 ◽

Vol 53 (1) ◽

Author(s):

Miguel Gozalo-Marcilla ◽

Jaap Buntjer ◽

Martin Johnsson ◽

Lorena Batista ◽

Federico Diez ◽

...

Keyword(s):

Candidate Genes ◽

Genetic Variance ◽

Mixed Model ◽

Linear Mixed Model ◽

Insulin Signalling ◽

Additive Genetic Variance ◽

Genomic Region ◽

Carcass Composition ◽

Backfat Thickness ◽

Genomic Regions

Abstract Background Backfat thickness is an important carcass composition trait for pork production and is commonly included in swine breeding programmes. In this paper, we report the results of a large genome-wide association study for backfat thickness using data from eight lines of diverse genetic backgrounds. Methods Data comprised 275,590 pigs from eight lines with diverse genetic backgrounds (breeds included Large White, Landrace, Pietrain, Hampshire, Duroc, and synthetic lines) genotyped and imputed for 71,324 single-nucleotide polymorphisms (SNPs). For each line, we estimated SNP associations using a univariate linear mixed model that accounted for genomic relationships. SNPs with significant associations were identified using a threshold of p < 10–6 and used to define genomic regions of interest. The proportion of genetic variance explained by a genomic region was estimated using a ridge regression model. Results We found significant associations with backfat thickness for 264 SNPs across 27 genomic regions. Six genomic regions were detected in three or more lines. The average estimate of the SNP-based heritability was 0.48, with estimates by line ranging from 0.30 to 0.58. The genomic regions jointly explained from 3.2 to 19.5% of the additive genetic variance of backfat thickness within a line. Individual genomic regions explained up to 8.0% of the additive genetic variance of backfat thickness within a line. Some of these 27 genomic regions also explained up to 1.6% of the additive genetic variance in lines for which the genomic region was not statistically significant. We identified 64 candidate genes with annotated functions that can be related to fat metabolism, including well-studied genes such as MC4R, IGF2, and LEPR, and more novel candidate genes such as DHCR7, FGF23, MEDAG, DGKI, and PTN. Conclusions Our results confirm the polygenic architecture of backfat thickness and the role of genes involved in energy homeostasis, adipogenesis, fatty acid metabolism, and insulin signalling pathways for fat deposition in pigs. The results also suggest that several less well-understood metabolic pathways contribute to backfat development, such as those of phosphate, calcium, and vitamin D homeostasis.

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PSVIII-10 Effects of enzyme supplementation in corn distiller’s dried grains with solubles (DDGS) diets on growth performance in broilers: a meta-analysis

Journal of Animal Science ◽

10.1093/jas/skaa054.365 ◽

2020 ◽

Vol 98 (Supplement_3) ◽

pp. 211-211

Author(s):

Jae-Cheol Jang ◽

Zhikai Zeng ◽

Pedro E Urriola ◽

Gerald C Shurson

Keyword(s):

Growth Performance ◽

Fixed Effects ◽

Mixed Model ◽

Linear Mixed Model ◽

Meta Analysis ◽

Growth Responses ◽

Exogenous Enzymes ◽

The Mean ◽

Enzyme Supplementation ◽

Mean Differences

Abstract The objective of this study was to conduct a meta-analysis to quantitatively summarize the growth responses of broilers fed cDDGS and the efficacy of various types of dietary enzyme supplementation. A total of 12 publications with 69 observations were included in the database. Individual observations were analyzed using a multivariable linear mixed model. The mean differences (MD) of BWG, FI, and gain efficiency (G/F) were calculated by subtracting either the enzyme response in corn-soybean meal (CSB) or CSB+cDDGS based diets to the control, and was expressed as a percentage (MD = (enzyme – control)/control ×100%). A type of exogenous enzymes (xylanase; protease; carbohydrases; cocktail = proteases + carbohydrases), and feeding phase (starter = d 0 to d 21; finisher = d 21 to d 42 or 49; overall = d 0 to d 42 or more) were included as fixed effects. Dietary enzyme inclusion showed significant improvement on BWG (3.19%, P < 0.01) and G/F (5.69%, P < 0.01) in broilers fed cDDGS diet. However, no significant enzyme responses were observed in broilers fed CSB diet on growth performance. Broilers fed cDDGS diet had increased (P < 0.01) BWG with the addition of protease (3.32 %) and cocktail (3.27 %), whereas addition of xylanased improved (P < 0.01) G/F by (3.56 %) and carbohydrases (1.90 %). Broilers fed cDDGS diet with enzyme supplementation showed greater improvement in BWG (3.71 %, P < 0.01) and G/F (3.78 %, P < 0.01) at finisher phase compared with starter phase. Likewise, Broilers fed CSB diet with enzyme supplementation increased BWG (9.40 %, P < 0.01) and G/F (3.11 %, P < 0.01) at finisher phase. In conclusion, supplementation of xylanase and carbohydrases in cDDGS diet improved G/F, and the enzyme response can be maximized when fed during the finisher phase diet compared with the starter phase diet.

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Corrigendum to: Performance and carcass composition of pigs from two sire lines are affected differently by ambient temperature

Animal Production Science ◽

10.1071/an20078_co ◽

2021 ◽

Vol 61 (6) ◽

pp. 620

Author(s):

Luan Sousa dos Santos ◽

Paulo Henrique Reis Furtado Campos ◽

Welex Cândido da Silva ◽

Alini Mari Veira ◽

Alicia Zem Fraga ◽

...

Keyword(s):

Ambient Temperature ◽

Growth Performance ◽

Carcass Characteristics ◽

Experimental Period ◽

Carcass Composition ◽

Environment Interaction ◽

Adaptation Period ◽

Genetic Line ◽

X Ray ◽

Individual Farm

Context Differences among breeds or lines of pigs in terms of growth and carcass characteristics may be affected by rearing environment (genetic × environment interaction). Aims The present study compared the growth performance and carcass composition of pigs from two sire lines reared under constant thermoneutral (22°C; TN) or high ambient temperature (33°C; HT) conditions. Methods Hampshire (HAM) and synthetic-cross (SYN) castrated male pigs (n = 12 per group; 32.0 ± 2.0 kg) were kept in individual pens at either 22°C (TN) or 33°C (HT) for 55 days (two experimental phases: 0–27 and 28–55 days) following an adaptation period of 7 days. Throughout the experimental period, growth performance and body composition (by dual-energy X-ray absorptiometry on Days 27 and 55) were assessed, and a range of other measurements (serum parameters and physiological responses), which were divided into four measurement groups, was taken on 9 days. Key results Irrespective of genetic line (G), the pigs in the HT treatment had lower average daily feed intake values (P P P P P Conclusion Despite progeny from both genetic lines being affected negatively by high AT, the purebred HAM pigs were less affected by the high AT conditions than were the pigs from the synthetic line. Implication The present findings suggest that individual farm conditions and AT are among the most important factors to consider before implementing a G.

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Adverse effects on growth performance and bone development in nursery pigs fed diets marginally deficient in phosphorus with increasing calcium to available phosphorus ratios

Journal of Animal Science ◽

10.1093/jas/skaa325 ◽

2020 ◽

Vol 98 (10) ◽

Author(s):

Spenser L Becker ◽

Stacie A Gould ◽

Amy L Petry ◽

Leah M Kellesvig ◽

John F Patience

Keyword(s):

Growth Performance ◽

Mixed Model ◽

Linear Mixed Model ◽

Bone Development ◽

Average Daily Gain ◽

Experimental Period ◽

Feed Ratio ◽

Available Phosphorus ◽

Nursery Pigs ◽

The Impact

Abstract The objective of this experiment was to evaluate the growth performance and bone mineral content (BMC) of nursery pigs in response to increasing total calcium (Ca) to available phosphorus (aP) ratios in diets containing phytase (250 FTU/kg; Natuphos E, BASF, Florham Park, NJ). A total of 480 nursery pigs (body weight (BW) = 5.7 ± 0.6 kg) with 10 pigs per pen and 7 pens per treatment (6 pens fed 2.75:1 diet) were allotted to seven treatments consisting of increasing ratios of calcium to available phosphorus (Ca:aP): 1.25, 1.50, 1.75, 2.00, 2.25, 2.50, and 2.75. From day −7 to 0, pigs were fed a common diet. They were then fed the treatment diets during two experimental phases from day 1 to 14 and 15 to 28, respectively. Available P was formulated to 0.33% and 0.27% (approximately 90% of requirement) in dietary phases 1 and 2, respectively. BW, average daily gain (ADG), average daily feed intake (ADFI), and gain-to-feed ratio (G:F) were determined. BMC of the femur was measured on day 28 on one pig per pen using dual x-ray absorptiometry. Data were analyzed as a linear mixed model using PROC MIXED (SAS, 9.3). Orthogonal polynomial contrasts were used to determine the linear and quadratic effects of increasing the Ca:aP. Over the 28-d experimental period, increasing Ca:aP resulted in a linear decrease in ADG (353, 338, 328, 304, 317, 291, and 280 g/d; P < 0.01), ADFI (539, 528, 528, 500, 533, 512, and 489 g/d; P < 0.05), and G:F (0.68, 0.66, 0.64, 0.62, 0.61, 0.59, and 0.58; P < 0.01). Increasing Ca:aP also resulted in decreased BW on days 14 and 28 (P < 0.01). The BMC of the femur decreased with increasing Ca:aP (6.2, 6.3, 5.7, 5.9, 5.5, 5.6, and 5.3 g; P < 0.05). Regression analysis explained the impact of Ca:aP as follows on ADG (ADG [g/d] = 339 − 36x; r2 = 0.81), G:F (G:F = 0.61 – 0.03x; r2 = 0.72), and BMC (BMC [g] = 6.4 – 0.27x; r2 = 0.43), where x is the Ca:aP. In conclusion, all outcomes indicated that any level of calcium above the minimum used in this experiment impaired growth performance and skeletal development. Further research using even lower levels of dietary Ca is warranted.

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Amino acid requirements of growing pigs 2. Identification of the limiting amino acid(s) in a low-protein diet supplemented with lysine

Animal Science ◽

10.1017/s0003356100025241 ◽

1981 ◽

Vol 33 (1) ◽

pp. 87-97 ◽

Cited By ~ 9

Author(s):

A. J. Taylor ◽

D. J. A. Cole ◽

D. Lewis

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Growth Performance ◽

Crude Protein ◽

Control Diet ◽

Live Weight ◽

Basal Diet ◽

Growing Pigs ◽

Carcass Composition ◽

Limiting Amino Acid

ABSTRACTA basal diet containing 120 g crude protein per kg and 9g lysine per kg, and previously shown to be limiting in one or more essential amino acids and/or non-essential nitrogen, was examined. It was fed either alone to growing female pigs from 25 kg to 55 kg live weight or in combination with four supplements of synthetic amino acids each containing three out of isoleucine, methionine, threonine and tryptophan. A control diet containing 140 g crude protein per kg and 9g lysine per kg was also included. Blood samples were collected at 40 kg live weight in order to examine the influence of dietary treatments on blood metabolites. Results for growth performance, carcass composition and blood urea indicated that threonine was the first limiting amino acid in the basal diet. Plasma free amino acids gave no clear trend. Growth performance and carcass composition were unaffected by supplementation of the diet with glycine indicating that the dietary supply of non-essential nitrogen was adequate.

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