scholarly journals Cricket (Gryllodes sigillatus) meal fed to healthy adult dogs does not affect general health and minimally impacts apparent total tract digestibility

2020 ◽  
Vol 98 (3) ◽  
Author(s):  
Logan R Kilburn ◽  
Anne T Carlson ◽  
Elizabeth Lewis ◽  
Mariana C Rossoni Serao
Keyword(s):  
Fixed Effects ◽  
Mixed Model ◽  
Healthy Adult ◽  
Biological Significance ◽  
Reference Intervals ◽  
Total Dietary Fiber ◽  
Study Objective ◽  
Fecal Output ◽  
Gryllodes Sigillatus ◽  
Collection Phase

Abstract Insects can serve as a novel high-quality protein source for pet foods. However, there is an absence of research investigating the use of insects in pet food. The study objective was to evaluate the apparent total tract digestibility and possible health effects of diets containing graded levels of cricket (Gryllodes sigillatus) meal fed to healthy adult dogs. Thirty-two adult Beagles were randomly assigned to one of four dietary treatments: 0%, 8%, 16%, or 24% cricket meal. Dogs were fed their respective diet for a total of 29 d with a 6-d collection phase. Fecal samples were collected daily during the collection phase to measure total fecal output as well as apparent total tract digestibility for dry matter (DM), organic matter, crude protein, fat, total dietary fiber, and gross energy. Blood samples were taken prior to the study and on day 29 for hematology and chemistry profiles. Data were analyzed in a mixed model including the fixed effects of diet and sex. Total fecal output increased on both an as-is (P = 0.030) and DM basis (P = 0.024). The apparent total tract digestibility of each nutrient decreased (P < 0.001) with the increasing level of cricket meal inclusion. All blood values remained within desired reference intervals indicating healthy dogs. Slight fluctuations in blood urea nitrogen (P = 0.037) and hemoglobin (P = 0.044) levels were observed but were not considered of biological significance. Even with the decrease in digestibility with the inclusion of cricket meal, diets remained highly digestible at greater than 80% total apparent digestibility. In conclusion, crickets were demonstrated to be an acceptable ingredient for dog diets.

PSV-B-18 Characterization of the fecal microbiome of healthy adult beagle dogs supplemented with graded levels of bacillus coagulans GBI-30, 6086

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 337-338
Author(s):  
Heather L Acuff ◽  
Tara N Gaire ◽  
Tyler Doerksen ◽  
Andrea Lu ◽  
Michael P Hays ◽  
...  
Keyword(s):  
Relative Abundance ◽  
Fixed Effects ◽  
Mixed Model ◽  
Healthy Adult ◽  
Random Effect ◽  
Bacillus Coagulans ◽  
Community Diversity ◽  
Rrna Gene ◽  
Beagle Dogs ◽  
Fecal Microbiome

Abstract This study aimed to evaluate the effect of Bacillus coagulans GBI-30, 6086 on the fecal microbiome of healthy adult dogs. Extruded diets containing graded levels of probiotic applied either to the base ration before extrusion or as a topical coating post-extrusion were randomly assigned to ten individually-housed Beagle dogs (7 castrated males, 3 spayed females) of similar age (5.75 ± 0.23 yr) and body weight (12.3 ± 1.5 kg) in a 5 x 5 replicated Latin square with 16-d adaptation and 5-d total fecal collection for each period. Five dietary treatments were formulated to deliver a dose of 0-, 6-, 7-, 8-, or 9-log10 CFU·dog-1·d-1. Fresh fecal samples (n=50) were analyzed by 16S rRNA gene pyrosequencing. Community diversity was evaluated in R (v4.0.3, R Core Team, 2019). Relative abundance data were analyzed using a mixed model (v9.4, SAS Institute, Inc., Cary, NC) with treatment and period as fixed effects and dog as a random effect. Results were considered significant at P < 0.05. Predominant phyla were Firmicutes (mean 81.2% ± 5), Actinobacteria (mean 9.9% ± 4.4), Bacteroidetes (mean 4.5% ± 1.7), Proteobacteria (mean 1.3% ± 0.7), and Fusobacteria (mean 1.1% ± 0.6). No evidence of shifts in predominant phyla, class, family, or genus taxonomic levels were observed except for the Bacillus genus, which had a greater relative abundance (P = 0.0189) in the low probiotic coating and high probiotic coating treatment groups compared to the extruded probiotic group. Alpha-diversity indices (Richness, Chao1, ACE, Shannon, Simpson, Inverse Simpson, and Fisher) and beta-diversity metrics (principal coordinate analysis and multi-dimensional scaling) were similar for all treatments. This data indicates that supplementation with Bacillus coagulans GBI-30, 6086 at a dose of up to 9 log10 CFU·d-1 did not alter the overall diversity of the fecal microbiome of healthy adult dogs over a 21-d period.

scholarly journals High-Fat Diets Led to OTU-Level Shifts in Fecal Samples of Healthy Adult Dogs

2020 ◽  
Vol 11 ◽  
Author(s):  
Logan R. Kilburn ◽  
Lucas R. Koester ◽  
Stephan Schmitz-Esser ◽  
Nick V. L. Serão ◽  
Mariana C. Rossoni Serão
Keyword(s):  
Fixed Effects ◽  
Dietary Intervention ◽  
Healthy Adult ◽  
Body Condition Score ◽  
Rrna Gene ◽  
High Fat ◽  
Study Objective ◽  
Fecal Samples ◽  
High Fat Diets ◽  
Fat Diets

High fat diets have been reported to negatively affect the microbiota in both mice and humans. However, there is a lack of studies in canine models. The variation among the gastrointestinal (GI) tract anatomy/physiology and typical diet compositions of these animal species may lead to vastly different results. Due to the large inclusion rate of dietary fat in pet food, it is critical to understand its effects in a canine model. Therefore, the study objective was to report the effects of high fat, low carbohydrate diets on the fecal microbiota in healthy adult dogs. Eight adult beagles were randomly assigned to one of four dietary treatments within each 15-day period of a replicated 4x4 Latin Square design. Diets contained 32% (T1), 37% (T2), 42% (T3), and 47% (T4) fat. T2, T3, and T4 were created by adding increasing levels of canola oil to T1, a commercially manufactured canned canine diet, which served as the control diet. Fresh fecal samples were collected during the last 5 days of each period for microbial analysis. DNA was extracted from fecal samples and paired-end 16S rRNA gene amplicon sequencing was performed using the Illumina MiSeq platform. When comparing whole microbial communities using PERMANOVA, no significant differences were observed among treatments (P = 0.735). Individual OTUs were analyzed using the GLIMMIX procedure of SAS with fixed effects of diet and room, and the random effects of period and animal. Out of the 100 most abundant individual OTUs, 36 showed significant differences in abundance based on treatment (q < 0.05). Overall, OTUs assigned to genera related to fat digestion increased while OTUs assigned to genera involved in carbohydrate digestion decreased. In conclusion, the microbial community adapted to dietary intervention without jeopardizing the health of the animals, evaluated by body condition score, fecal characteristics, and blood parameters.

scholarly journals Metabolic Adaptations to Dynamic Energy Requirements during Lactation and Pregnancy in Dairy Cows with Varying Proportions of Holstein and Simmental Breed

Proceedings ◽  
2020 ◽  
Vol 73 (1) ◽  
pp. 9
Author(s):  
Deise Aline Knob ◽  
André Thaler Neto ◽  
Helen Schweizer ◽  
Anna Weigand ◽  
Roberto Kappes ◽  
...  
Keyword(s):  
Energy Balance ◽  
Dairy Cows ◽  
Fixed Effects ◽  
Mixed Model ◽  
Negative Energy ◽  
Negative Energy Balance ◽  
Repeated Measure ◽  
Holstein Cows ◽  
Mixed Model Analysis ◽  
Genetic Groups

Depending on the breed or crossbreed line, cows have to cope with a more or less severe negative energy balance during the period of high milk yields in early lactation, which can be detected by beta-hydroxybutyrate (BHBA) and non-esterified fatty acids (NEFAs) in blood. Preventing cows from undergoing a severe negative energy balance by breeding and/or feeding measures is likely to be supported by the public and may help to improve the sustainability of milk production. The aim was to compare BHBA and NEFA concentrations in the blood of Holstein and Simmental cows and their crosses during the prepartum period until the end of lactation. In total, 164 cows formed five genetic groups according to their theoretic proportion of Holstein and Simmental genes as follows: Holstein (100% Holstein; n = 9), R1-Hol (51–99% Holstein; n = 30), F1 crossbreds (50% Holstein, 50% Simmental; n = 17), R1-Sim (1–49% Holstein; n = 81) and Simmental (100% Simmental; n = 27). NEFA and BHBA were evaluated once a week between April 2018 and August 2019. A mixed model analysis with fixed effects breed, week (relative to calving), the interaction of breed and week, parity, calving year, calving season, milking season, and the repeated measure effect on cows was used. Holstein cows had higher NEFAs (0.196 ± 0.013 mmol/L), and Simmental cows had the lowest NEFA concentrations (0.147 ± 0.008 mmol/L, p = 0.03). R1-Sim, F1 and R1-Hol cows had intermediate values (0.166 ± 0.005, 0.165 ± 0.010, 0.162 ± 0.008 mmol/L; respectively). The highest NEFA value was found in the first week after calving (0.49 ± 0.013 mmol/L). BHBA did not differ among genetic groups (p = 0.1007). There was, however, an interaction between the genetic group and week (p = 0.03). While Simmental, R1-Sim and F1 cows had the highest BHBA value, the second week after calving (0.92 ± 0.07 and 1.05 ± 0.04, and 1.10 ± 0.10 mmol/L, respectively), R1-Hol and Holstein cows showed the BHBA peak at the fourth week after calving (1.16 ± 0.07 and 1.36 ± 0.12 mmol/L, respectively). Unexpectedly, Holstein cows had a high BHBA peak again at week 34 after calving (1.68 ± 0.21 mmol/L). The genetic composition of the cows affects NEFA and BHBA. Simmental and R1-Sim cows mobilize fewer body reserves after calving. Therefore, dairy cows with higher degrees of Simmental origin might be more sustainable in comparison with Holstein genetics in the present study.

Ethnic variation in certain hematological and biochemical reference intervals in a south Indian healthy adult population

2008 ◽  
Vol 19 (1) ◽  
pp. 46-50 ◽  
Author(s):  
M. Sundaram ◽  
J. Mohanakrishnan ◽  
K.G. Murugavel ◽  
E.M. Shankar ◽  
S. Solomon ◽  
...  
Keyword(s):  
Healthy Adult ◽  
Adult Population ◽  
Reference Intervals ◽  
Ethnic Variation ◽  
South Indian

PSV-11 The effects of pelleting and extrusion on nutrient composition and protein quality measurements in a variety Canadian pulses

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 302-302
Author(s):  
Cara Cargo-Froom ◽  
Anna-Kate Shoveller ◽  
Daniel A Columbus ◽  
Chris Marinangeli ◽  
Elijah Kiarie ◽  
...  
Keyword(s):  
Protein Content ◽  
Faba Bean ◽  
Fixed Effects ◽  
Crude Protein ◽  
Mixed Model ◽  
Protein Quality ◽  
Positive Impact ◽  
Nutrient Composition ◽  
Crude Protein Content ◽  
Quality Measurements

Abstract Alternative forms of protein are an important focus in nutrition. This study sought to compare the effects of pelleting and extrusion on nutrient composition and protein quality measurements of Canadian pulses. Pulses used for the study included: 2 pea variety (Amarillo and dunn), lentils, chickpeas, and faba bean. Ingredients were ground through a 10/64” or a 2/64” screen to create a coarse and fine ground product, respectively. Both coarse and fine ground ingredients were pelleted at 60–65, 70–75, and 80–85 C0. Fine ground ingredients were extruded at three different temperatures (110, 130, 150 C0) and two moisture levels (18 and 22%). Samples were collected for all runs at the beginning, middle, and end of each run for both pelleted and extruded samples. Samples were analyzed for proximate analysis, amino acids including lysinoalanine, total and damaged starch, and total dietary fibre (including insoluble and soluble). Data were analyzed using a mixed model via proc glimmix in SAS, where ingredient, process, grind, temperature, and extrusion moisture were treated as fixed effects with different interactions selected based on model investigated. Crude protein content of whole pulses was highest in faba bean and lowest in the Amarillo pea, with faba bean protein content significantly higher than all other pulses, and lentil protein content significantly higher than Amarillo peas (P < 0.05). All pelleting temperatures, nested within grind, significantly increased crude protein content of all pulses compared to whole pulses (P < 0.05). All extrusion moistures significantly increased crude protein content of all pulses compared to whole pulses (P < 0.05) and moisture/temperature interactions were significantly higher for all pulses compared to whole pulses (P < 0.05). Amino acid comparisons produced similar significant results. This suggests that pelleting and extrusion processing can have a positive impact on protein content of pulses and protein quality measurements in pulses.

Evaluation of genetic markers for litter size in Meishan synthetic and Large White pigs

1996 ◽  
Vol 1996 ◽  
pp. 18-18
Author(s):  
O.I. Southwood ◽  
S. Hoste ◽  
T.H. Short ◽  
A.J. Mileham ◽  
D. Cuthbert-Heavens
Keyword(s):  
Litter Size ◽  
Fixed Effects ◽  
Oestrogen Receptor ◽  
Mixed Model ◽  
Receptor Gene ◽  
Large White ◽  
Service Type ◽  
Sib Families ◽  
Natural Service ◽  
Size Data

A significant relationship between the oestrogen receptor gene (ESR) and litter size has been detected in USA populations of Large White and a synthetic comprising 50% Meishan (Rothschild et al., 1995). Animals carrying two copies of the favourable allele (B) had an extra pig born per litter than those that did not have the allele. This paper reports on results observed in a UK 50% Meishan synthetic and four UK Large White lines.Litter size data from 50% Meishan synthetic (L93) full-sib females where more than one ESR genotype was segregating. Data were analysed using a mixed model with full relationships and including the fixed effects of season of farrowing, parity, ESR genotype (AA, AB or BB) and service type (AI or natural service). Heritiability and permanent environmental effects for litter size were assumed as 0.09 and 0.11, repectively. A total of 27 full-sib families were represented and included 62 sows and 139 litter records. Hypothesis testing used the option in PEST under a mixed model (Groeneveld et al., 1991).

scholarly journals Goats Given Transdermal Flunixin Meglumine Displayed Less Pain Behavior After Castration

2020 ◽  
Author(s):  
Amanda Lee ◽  
Meggan Graves ◽  
Andrea Lear ◽  
Sherry Cox ◽  
Marc Caldwell ◽  
...  
Keyword(s):  
Fixed Effects ◽  
Mixed Model ◽  
Linear Mixed Model ◽  
Body Position ◽  
Random Effect ◽  
Pain Behavior ◽  
Bout Duration ◽  
Flunixin Meglumine ◽  
Meat Goats ◽  
Transdermal Application

AbstractPain management should be utilized with castration to reduce physiological and behavioral changes. Transdermal application of drugs require less animal management and fewer labor risks, which can occur with oral administration or injections. The objective was to determine the effects of transdermal flunixin meglumine on meat goats’ behavior post-castration. Male goats (N = 18; mean body weight ± standard deviation: 26.4 ± 1.6 kg) were housed individually in pens and randomly assigned to 1 of 3 treatments: (1) castrated, dosed with transdermal flunixin meglumine; (2) castrated, dosed with transdermal placebo; and (3) sham castrated, dosed with transdermal flunixin meglumine. Body position, rumination, and head- pressing were observed for 1 h ± 10 minutes twice daily on days −1, 0, 1, 2, and 5 around castration. Each goat was observed once every 5-minutes (scan samples) and reported as percentage of observations. Accelerometers were used to measure standing, lying, and laterality (total time, bouts, and bout duration). A linear mixed model was conducted using GLIMMIX. Fixed effects of treatment, day relative to castration, and treatment*day relative to castration and random effect of date and goat nested within treatment were included. Treatment 1 goats (32.7 ± 2.8%) and treatment 2 goats (32.5 ± 2.8%) ruminated less than treatment 3 goats (47.4 ± 2.8%, P = 0.0012). Head pressing was greater on day of castration in treatment 2 goats (P < 0.001). Standing bout duration was greatest in treatment 2 goats on day 1 post-castration (P < 0.001). Lying bout duration was greatest in treatment 2 goats on day 1 post-castration compared to treatment 1 and treatment 3 goats(P < 0.001). Transdermal flunixin meglumine improved goats’ fluidity of movement post-castration and decreased head pressing, indicating a mitigation of pain behavior.

scholarly journals The role of incubator classification on performance of incubators in Kenya.

2021 ◽  
Vol 10 (6) ◽  
pp. 256-267
Author(s):  
Miriti Jane Kinya ◽  
Kenneth Lawrance Wanjau ◽  
Nyagweth Ebenezer Odeyo
Keyword(s):  
Fixed Effects ◽  
Selection Criteria ◽  
Primary Data ◽  
Cross Sectional ◽  
Mixed Effect ◽  
Study Objective ◽  
Mixed Effect Models ◽  
Random Slopes ◽  
Linear Mixed Effect Models

The study sought to assess the importance of classifying incubators based on the programs offered for optimum performance. Client selection criteria were assessed through three constructs namely: models that fit program goals, uniqueness of ideas, and standard selection tool. A mixed cross-sectional and causal design was adopted and a census was carried out targeting all the 51 incubators. Primary data was collected with an incubator program as a grouping/ cluster variable yielding a multilevel data structure with incubator centres nested in programs. Linear mixed effect models were fitted using Stata to assess the study objective taking into account the fixed effects for the incubator centre level (level-1) and random effects for the program level (level-2). The uniqueness of ideas was found to have a significant fixed effect on performance at level one while at level two, the study found significant random intercepts of incubator centre performance across the programs. Models that match program goals and standard selection tools were also found to have significant random slopes as level two random covariates in the model. Based on the findings of significant random slopes, the study concluded that incubator classification is key for client selection criteria and enhances incubator performance.

scholarly journals The Impact of Misspecified Random Effect Distribution in a Weibull Regression Mixed Model

Stats ◽  
2018 ◽  
Vol 1 (1) ◽  
pp. 48-76
Author(s):  
Freddy Hernández ◽  
Viviana Giampaoli
Keyword(s):  
Weibull Distribution ◽  
Random Effects ◽  
Mixed Models ◽  
Fixed Effects ◽  
Mixed Model ◽  
Random Effect ◽  
Estimation Procedure ◽  
Weibull Regression ◽  
Two Parameters ◽  
The Impact

Mixed models are useful tools for analyzing clustered and longitudinal data. These models assume that random effects are normally distributed. However, this may be unrealistic or restrictive when representing information of the data. Several papers have been published to quantify the impacts of misspecification of the shape of the random effects in mixed models. Notably, these studies primarily concentrated their efforts on models with response variables that have normal, logistic and Poisson distributions, and the results were not conclusive. As such, we investigated the misspecification of the shape of the random effects in a Weibull regression mixed model with random intercepts in the two parameters of the Weibull distribution. Through an extensive simulation study considering six random effect distributions and assuming normality for the random effects in the estimation procedure, we found an impact of misspecification on the estimations of the fixed effects associated with the second parameter σ of the Weibull distribution. Additionally, the variance components of the model were also affected by the misspecification.

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