271 Investigating the Relationship Between Nursery Pig Performance and Markers of Intestinal Morphology and Integrity

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 100-101
Author(s):  
Carson M De Mille ◽  
Nicholas K Gabler
Keyword(s):  
Active Transport ◽  
Feed Intake ◽  
Ex Vivo ◽  
Intestinal Morphology ◽  
Ussing Chambers ◽  
Nursery Pig ◽  
Pig Performance ◽  
Positive Correlations ◽  
And Function ◽  
Time Point

Abstract Weaning induces major structural and function changes to the small intestine of pigs and they transition from milk to solid feedstuffs. Thus, the objective of this study was to determine how intestinal morphology and function markers relate to feed intake and growth rates of nursery pig. Forty-eight weaned pigs (5.63 ± 0.50 kg) were randomly selected, individually penned and fed a common diet. Pig bodyweights and feed intake were determined at d 2, 7, and 21. At each time point, 16 pigs were randomly selected and euthanized. Sections of ileum were assessed for morphology [villus height (VH), crypt depth (CD) and VH:CD] and ex vivo transepithelial resistance (TER), macromolecule permeability (FD4), and active transport of glucose and glutamine via modified Ussing chambers. Within each period (d 0–2, 0–7, and 0–21), Pearson correlations were performed between ADG, ADFI, VH, VH:CD, TER, FD4 and active transport of glucose and glutamine. At d 2 post-weaning, no correlations (P > 0.05) were observed between performance and intestinal variables. By d 7, moderate positive correlations between VH and ADFI (r = 0.69, P = 0.005), VH and ADG (r = 0.68, P = 0.006) were reported. At 21 d post-weaning, moderate positive correlations were still observed for VH and ADFI (r = 0.55, P = 0.026) and between VH and ADG (r = 0.51, P = 0.042). Interestingly, ADFI and ADG tended to be negatively correlated with active glucose transport (r = -0.45, P = 0.083 and r = -0.47, P = 0.064, respectively) and active glutamine transport (r = -0.45, P = 0.083 and r = -0.46, P = 0.073, respectively). Markers of ileal integrity (TER and FD4) were not correlated with ADG or ADFI at any time point. Altogether, these data highlight the importance of intestinal morphology on early nursery pig performance.

scholarly journals PSIV-18 Effect of sub-therapeutic antibiotics and pharmacological zinc oxide on nursery pig performance and intestinal integrity and function

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 190-190
Author(s):  
Carson De Mille ◽  
Emma T Helm ◽  
Crystal L Loving ◽  
Eric R Burrough ◽  
Nicholas K Gabler
Keyword(s):  
Zinc Oxide ◽  
T Cell ◽  
Ex Vivo ◽  
Ussing Chambers ◽  
Cell Abundance ◽  
Intestinal Integrity ◽  
Nursery Pig ◽  
Pig Performance ◽  
And Function ◽  
The Impact

Abstract The objective of this study was to evaluate the impact of sub-therapeutic chlortetracycline (CTC) and pharmacological zinc oxide on nursery pig performance and intestinal integrity and function. Twenty-four newly weaned pigs (4.51 ± 0.21 kg BW) were randomly assigned to one of three dietary treatments for 21 d (n = 8/treatment): 1) Control diet (NC), 2) NC + Zinc oxide at 3000 ppm for 7 d followed by 2000 ppm for 14 d (ZnO), and 3) NC + 40 ppm CTC for 21 d (sCTC). Pig ADG, ADFI, and G:F were determined for the overall 21 d test period. At d 21, all pigs were euthanized for tissue collection. Jejunum and ileum ex vivo transepithelial resistance (TER), macromolecule (FD4) flux, and active glucose and glutamine transport were assessed in modified Ussing Chambers. Additionally, jejunum brush border enzyme and Na+/K+ ATPase activities were determined. Morphology and T-cell abundance were determined in all ileum sections by H&E staining and immunohistochemistry, respectively. Compared to the NC, sCTC and ZnO increased ADG (0.14, 0.20 and 0.23 kg/d, respectively, P = 0.020). Overall ADFI was increased by 150% due to sCTC compared to the NC and ZnO pigs (P < 0.05). G:F was increased due to ZnO and sCTC compared to the NC (0.82, 0.63 and 0.47, respectively, P < 0.01). Jejunum and ileum ex vivo function and integrity did not differ. Ileal villus height was increased in the NC compared to sCTC treatment (P < 0.01), but not different from the ZnO treatment. In the ileum, total T-cell number per unit area was significantly lower in the ZnO segments compared to sCTC and NC (0.95, 2.29 and 2.59 count per μm2, respectively, P = 0.05). These data indicate that ZnO and sCTC improved pig performance but did not directly alter small intestinal integrity and function. However, mucosal T cell abundance was altered.

268 In-feed Antibiotics Elicit Intestinal Integrity Modifications Early in Post-weaning Life

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 99-99
Author(s):  
Jessica M Johnson ◽  
Emma T Helm ◽  
Nicholas K Gabler ◽  
Eric R Burrough ◽  
Carson M De Mille
Keyword(s):  
Growth Performance ◽  
Fixed Effects ◽  
Ex Vivo ◽  
Phase 1 ◽  
Physiological Mechanisms ◽  
Nursery Pigs ◽  
Ussing Chambers ◽  
Intestinal Integrity ◽  
And Function ◽  
Dietary Treatments

Abstract The physiological mechanisms by which in-feed antibiotics improve pig growth performance are largely unknown. One proposed mode of action is improvements in intestinal integrity and function. Therefore, the objective of this study was to test the hypothesis that in-feed therapeutic and sub-therapeutic antibiotics would improve intestinal integrity and function in nursery pigs. Twenty-four weaned pigs (6.1±1.1 kg BW) were randomly allotted to individual pens and assigned one of three dietary treatments as follows (n = 8 pigs/trt): 1) control, no antibiotics (CON), 2) CON + sub-therapeutic chlortetracycline [40 ppm in feed (sCTC)], and 3) CON + chlortetracycline-tiamulin [400 ppm + 35 ppm, respectively (CTCDen)]. The study consisted of two consecutive 14 d phases. Chlortetracycline-tiamulin was only fed in phase 1, sCTC was fed in both phases. Phase 1 and 2 ADG, ADFI, and G:F were determined. After 28 d, ileal and colonic ex vivo intestinal integrity was assessed via transepithelial resistance (TER) and macromolecule flux (FD4) in modified Ussing chambers. All data were analyzed for the fixed effects of treatment and start BW as a covariate. In phase 1, compared with CON and sCTC, CTCDen tended to have greater ADG (0.28, 0.31, and 0.33 kg/d, respectively, P = 0.10) and ADFI (0.28, 0.30, and 0.35 kg/d, respectively, P = 0.09). No differences in phase 1 G:F were observed (P = 0.11). Phase 2 ADG, ADFI, and G:F did not differ (P &gt; 0.10). Further, ileal TER and FD4 did not differ (P &gt; 0.10). Colonic TER tended to be increased in sCTC compared with CON and CTCDen (78, 56, and 59 Ω/cm2, respectively, P = 0.07). Compared with CON, colonic FD4 flux was decreased in sCTC and CTCDen by 35–40% (P = 0.03). Altogether, these data indicate that in-feed antibiotics improve colon integrity early in production which may contribute to improved growth performance.

Supplement containing immunoglobulins fed post weaning promotes nursery pig performance

2000 ◽  
Vol 2000 ◽  
pp. 106-106 ◽  
Author(s):  
H.M. Miller ◽  
P. Toplis
Keyword(s):  
Growth Rate ◽  
Feed Intake ◽  
Nutrient Intake ◽  
Weaning Weight ◽  
Nursery Pig ◽  
Pelleted Feed ◽  
Pig Performance

Post weaning performance is determined by weaning weight and week one growth rate (Miller, et al., 1999). Strategies which increase feed intake in the immediate post-weaning period would be expected to increase overall post-weaning performance through better week one growth rate. Top dressing normal pelleted feed following weaning with a palatable nutrient dense supplement should increase nutrient intake and hence week 1 growth rate. This strategy might be particularly beneficial for low weaning weight pigs. Inclusion of ingredients containing immunoglobulins (Ig) in immediate post-weaning diets have been shown to increase post weaning performance (Coffey and Cromwell, 1995). We hypothesise that the use of a palatable nutrient dense supplement containing bovine Igs will improve feed intake and growth rate post weaning. The aim of this experiment was to investigate this hypothesis using low weaning weight piglets.

scholarly journals Mycoplasma hyopneumoniae–Lawsonia intracellularis dual challenge modulates intestinal integrity and function1

2019 ◽  
Vol 97 (6) ◽  
pp. 2376-2384 ◽  
Author(s):  
Emma T Helm ◽  
Shelby M Curry ◽  
Kent J Schwartz ◽  
Steven M Lonergan ◽  
Nicholas K Gabler
Keyword(s):  
Ex Vivo ◽  
Digestive Enzyme ◽  
Random Effect ◽  
Mycoplasma Hyopneumoniae ◽  
Intestinal Function ◽  
Lawsonia Intracellularis ◽  
Colon Tissue ◽  
Transport Barrier ◽  
Ussing Chambers ◽  
Pig Performance

Abstract Lawsonia intracellularis (LI) and Mycoplasma hyopneumoniae (Mh) are 2 globally distributed pathogens that cause significant morbidity and mortality in grow-finish pigs. However, mechanisms that reduce growth and feed efficiency during LI and Mh infection are poorly defined. We hypothesized that reductions in performance are partially due to declines in intestinal function and integrity; thus, this study aimed to evaluate intestinal function and integrity of pigs during a 21-d Mh and LI dual challenge (MhLI). Littermate pairs of barrows (48.1 ± 6.7 kg BW) were selected; 1 pig from each pair was assigned to either MhLI challenge or nonchallenge treatments (n = 12). Pigs were individually housed, fed a corn-soybean diet, and allowed to acclimate for 21 d prior to inoculation. On days postinoculation (dpi) 0, MhLI pigs were dual inoculated with LI and Mh. On dpi 21, all pigs were euthanized for ileal and colon tissue collection. Formalin-fixed tissues were clinically scored and morphology analyzed, frozen tissues assayed for digestive enzyme activities, and fresh tissues mounted into modified Ussing Chambers to assess active nutrient transport, barrier integrity, and bacterial translocation. Data were analyzed using the Mixed Procedure of SAS with treatment as a fixed effect, age and start BW as covariates, and litter as a random effect. Compared with controls, MhLI pigs had decreased ADG (38%, P &lt; 0.001), ADFI (25%, P &lt; 0.001), and G:F (19%, P = 0.012). The MhLI dual challenge did not alter ileum morphology or transepithelial resistance (P &gt; 0.10); however, ex vivo mucosal to serosal translocation of S. Typhimurium in the colon was increased (60%, P = 0.003) in MhLI pigs compared with controls. Additionally, MhLI pigs had increased ileal glucose transport (30%, P = 0.05) and decreased sucrase activity (30%, P = 0.049) compared with controls. This MhLI challenge antagonized intestinal function and integrity, and this may be a contributing factor to reduced pig performance.

PSV-17 Evaluation of the Effects of a Feed Sweetener on Nursery Pig Performance

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 190-191
Author(s):  
Thomas E Weber ◽  
Julie Salyer ◽  
Audrey Walter ◽  
Yan Lei ◽  
Yulin Ma ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Growth Performance ◽  
Feed Intake ◽  
Phase 1 ◽  
Negative Control ◽  
Phase 2 ◽  
Treatment Groups ◽  
Feeding Program ◽  
Nursery Pig ◽  
Pig Performance

Abstract Two studies were conducted to evaluate the effects of a feed sweetener on nursery pig performance. In the first study, 360 pigs (PIC 800 x 1050; 6.22 ± 1.20 kg) were blocked by BW and pen location and assigned to 3 treatment groups: 1) Control; 2) 100 mg/kg feed sweetener; or 3) 200 mg/kg feed sweetener. There were 12 pens containing 10 pigs for each treatment. In the second study, 540 pigs (6.12 ± 0.39 kg) were blocked by BW and pen location and assigned to 3 treatment groups: 1) negative control; 2) 100 mg/kg feed sweetener; or 3) 0.5% carob bean. There were 18 pens containing 10 pigs per pen for each treatment. A 3-phase feeding program was used, and diets were fed for 6 wk in both studies. In study 1, ADFI, ADG, and gain:feed were increased (P &lt; 0.05) during Phase 1 for pigs fed diets with 100 mg/kg or 200 mg/kg sweetener. Phase 2 ADFI and ADG were increased (P &lt; 0.05) in pigs fed 100 mg/kg sweetener when compared to the control. Increased BW (P &lt; 0.05) were observed on D 7, 14 and 21 for animals fed 100 mg/kg or 200 mg/kg sweetener. The mortality rate was decreased (P &lt; 0.05) in the group of animals fed 100 mg/kg sweetener compared to the control. In study 2, there was no effect of sweetener or carob bean on growth performance, feed intake or gain:feed. However, mortality rate was decreased (P &lt; 0.05) in pigs fed the sweetener compared to pigs fed carob bean. In conclusion, the feed sweetener improved early nursery performance in one study and had no effect in another study. Mortality was decreased by supplementation with 100 mg/kg sweetener when compared to a negative control in one study and compared pigs fed carob bean in the second study. Further research is needed to explore differences observed between studies to optimize feed sweetener usage strategy.

scholarly journals 364 Impact of porcine reproductive and respiratory syndrome virus challenge and deoxynivalenol on intestinal integrity and nutrient transport

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 148-149
Author(s):  
Samuel Gerrard ◽  
Emma T Helm ◽  
Blaire Todd ◽  
Nicholas K Gabler
Keyword(s):  
Glucose Transport ◽  
Fixed Effects ◽  
Ex Vivo ◽  
Respiratory Syndrome Virus ◽  
Ussing Chambers ◽  
Prrs Virus ◽  
Intestinal Integrity ◽  
Glutamine Transport ◽  
And Function ◽  
The Impact

Abstract Porcine reproductive and respiratory syndrome (PRRS) virus infections and mycotoxin contaminated feedstuffs are two common issues facing the pork industry as both act, independently, to antagonize pig intestinal function, growth performance, and health. However, the combined impact of PRRS and mycotoxin exposure on pig intestinal health and function has not been investigated. Therefore, the objective of this experiment was to examine the impact of the mycotoxin deoxynivalenol (DON) on the intestinal integrity and function of PRRS infected pigs, employing an ex vivo model. Jejunum samples were collected from PRRS naïve (control; n = 6) and 21 day post inoculated PRRS virus infected (n = 8) gilts (10 weeks of age). In duplicate, explants were mounted into modified Ussing Chambers and incubated with or without 25 mM DON for 45 minutes prior to assessment of transepithelial resistance (TER), mucosal to serosal macromolecule (FD4) flux, and active glucose and glutamine transport. Data were analyzed using the mixed model procedure of SAS and a split plot design to examine the fixed effects of PRRS, DON, and their interaction. There were no PRRS by DON interactions for any ex vivo parameters assessed. Irrespective of DON, PRRS jejunum segments had decreased TERs (57%, P < 0.001), increased FD4 flux (P = 0.005), and tended to have increased active glucose transport (P = 0.093) compared with uninfected controls. Irrespective of PRRS status, DON increased FD4 flux (113%, P = 0.013), and tended to increase active glucose transport (P = 0.088) compared with jejunal explants not treated with DON. However, TERs and active glutamine transport did not differ due to DON treatment. Taken together, these data highlight the impacts that viral health challenge and mycotoxin contamination have on aspects of intestinal integrity and function. However, it does not appear that PRRS challenged pigs are more sensitive to the effects of DON than their healthy counterparts.

Effect of a Non-antibiotic Feed Additive (Royal Nutrazyme™) on Nursery Pig Performance

2005 ◽  
Author(s):  
David R. Stender ◽  
Jerry Weiss ◽  
Dennis DeWitt ◽  
Colin D. Johnson ◽  
Kenneth J Stalder ◽  
...  
Keyword(s):  
Feed Additive ◽  
Nursery Pig ◽  
Pig Performance

PENGARUH PENGGUNAAN VIRGIN COCONUT OIL (VCO) DALAM RANSUM TERHADAP PERFORMANS TERNAK BABI FASE STARTER

ZOOTEC ◽  
2015 ◽  
Vol 35 (2) ◽  
pp. 86
Author(s):  
Alfred Tamawiwy ◽  
M. Najoan ◽  
J S Mandey ◽  
F. N Sompie
Keyword(s):  
Water Consumption ◽  
Feed Intake ◽  
Control Diet ◽  
Coconut Oil ◽  
Growing Pigs ◽  
Virgin Coconut Oil ◽  
Daily Water ◽  
Pig Performance ◽  
Daily Gain ◽  
Daily Water Consumption

ABSTRACT   EFFECT OF UTILIZATION OF VIRGIN COCONUT OIL (VCO) IN THE DIET ON PIG PERFORMANCE. Utilization of fats and oils in pig diets is of great importance due to their high energy value. VCO is obtained by cold press processing of the kernel from the coconut fruit. Utilization of virgin coconut oil (VCO) in the diets on pig performance.  The present study was designed to elaborate the effect of utilization of VCO in the diets on energy and protein digestibility of growing pigs. The experiment was conducted using 20 castrated male pigs aged 1.5 - 2.0 months weighing 12,0±2,0 kg. The data were analyzed according to the linear model procedure for ANOVA appropriate for Randomized Block Design with 5 treatments and 4 replications. Treatments were formulated as follow: R0 = 100% control diet + 0% VCO; R1 = 99.5% control diet + 1.0% VCO; R2 = 98.0% control diet + 2.0% VCO; R3 = 97.0% control diet + 3.0% VCO; and R4 = 96.0% control diet + 4.0% VCO. Parameters measured were: daily feed intake, daily gain, daily water consumption. The results showed that the utilization of VCO up to 4% in the diets had no significant effect (P > 0.05) on daily feed intake, daily gain, daily water consumption of pigs. It can be concluded that the addition of VCO up to 4.0% in the diets has no significant meaning on pig performance.   Key words: Virgin coconut oil (VCO), Performance, Growing pigs  

scholarly journals Cadherins in early neural development

2021 ◽  
Author(s):  
Karolina Punovuori ◽  
Mattias Malaguti ◽  
Sally Lowell
Keyword(s):  
Adhesion Molecules ◽  
Cell Fate ◽  
Neural Development ◽  
Ex Vivo ◽  
Directed Differentiation ◽  
Culture Dish ◽  
Cell Identity ◽  
Cell Fate Decisions ◽  
Neural Tissues ◽  
And Function

AbstractDuring early neural development, changes in signalling inform the expression of transcription factors that in turn instruct changes in cell identity. At the same time, switches in adhesion molecule expression result in cellular rearrangements that define the morphology of the emerging neural tube. It is becoming increasingly clear that these two processes influence each other; adhesion molecules do not simply operate downstream of or in parallel with changes in cell identity but rather actively feed into cell fate decisions. Why are differentiation and adhesion so tightly linked? It is now over 60 years since Conrad Waddington noted the remarkable "Constancy of the Wild Type” (Waddington in Nature 183: 1654–1655, 1959) yet we still do not fully understand the mechanisms that make development so reproducible. Conversely, we do not understand why directed differentiation of cells in a dish is sometimes unpredictable and difficult to control. It has long been suggested that cells make decisions as 'local cooperatives' rather than as individuals (Gurdon in Nature 336: 772–774, 1988; Lander in Cell 144: 955–969, 2011). Given that the cadherin family of adhesion molecules can simultaneously influence morphogenesis and signalling, it is tempting to speculate that they may help coordinate cell fate decisions between neighbouring cells in the embryo to ensure fidelity of patterning, and that the uncoupling of these processes in a culture dish might underlie some of the problems with controlling cell fate decisions ex-vivo. Here we review the expression and function of cadherins during early neural development and discuss how and why they might modulate signalling and differentiation as neural tissues are formed.

scholarly journals Effect of the Pellet and Mash Feed Forms on the Productive Performance, Egg Quality, Nutrient Metabolism, and Intestinal Morphology of Two Laying Hen Breeds

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 701
Author(s):  
Yi Wan ◽  
Ruiyu Ma ◽  
Anam Khalid ◽  
Lilong Chai ◽  
Renrong Qi ◽  
...  
Keyword(s):  
Feed Intake ◽  
Laying Hens ◽  
Egg Quality ◽  
Laying Hen ◽  
Intestinal Morphology ◽  
Production Performance ◽  
Apparent Digestibility ◽  
Villus Height ◽  
Nutrient Metabolism ◽  
Feed Form

One of the most important factors that determine feed utilization by chickens is the feed form. Although it is generally believed that pellet diets have a positive effect on chicken growth, there are some studies that have indicated no difference between pellet and mash on chickens performance. This study was conducted to assess the effects of feed form on production performance, egg quality, nutrient metabolism and intestinal morphology in two breed laying hens. Two hundred and sixteen 25-week-old Hy-Line brown (n = 108) and Hy-Line grey (n = 108) hens were selected. Each breed was randomly allocated into two treatments with 6 replications (9 birds in each replication), which were fed mash and pellet diets, respectively. Production performances were recorded daily and egg quality traits were measured every two weeks. At 42 weeks of age, one bird per replication from each experimental group was selected for metabolism determination and intestine morphology observation. Compared with mash diets, pellet diets improved laying rate (p < 0.05), ADFI (average daily feed intake, p < 0.05), egg weight, shell strength, yolk proportion and Haugh unit (p < 0.05) in both breeds and reduced the FCR (feed conversion ratio, p < 0.05) in Hy-Line grey. The apparent digestibility of DM% (dry matter) and CP% (crude protein) were significantly higher (p < 0.05) in both breed laying hens fed pellet than those fed mash. The apparent digestibility of P% (phosphorus) and Ca% (calcium) was higher in Hy-Line grey fed pellet and was higher in Hy-Line brown fed mash. Compared to mash diets, pellet diets increased the VH (villus height), CD (crypt depth) and VCR (ratio of villus height to crypt depth) of the small intestine of Hy-Line grey, and increased the VH and CD of duodenum and ileum of Hy-Line brown. Overall, pellet diets improved production performance and nutrition metabolism through positive changes in the laying rate, feed intake, egg albumen quality and apparent digestibility of laying hens. The current findings provided support for the advantages of feeding pellets during the peak egg laying period for the two popular laying hen strains, Hy-Line brown and Hy-Line grey.

Sign in / Sign up

Export Citation Format

Share Document