219 Effects of Feeding Low Protein Diets with Additional Supplementation of First Five Limiting AA on Performance and Diarrhea Incidence in Weaned Pigs

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 114-115
Author(s):  
Natasha Van Niekerk ◽  
John K Htoo ◽  
Caroline Gonzalez Vega
Keyword(s):  
Crude Protein ◽  
Phase 1 ◽  
Diet Analysis ◽  
Negative Effects ◽  
Weaned Pigs ◽  
Low Protein ◽  
Diarrhea Incidence ◽  
Pig Performance ◽  
Protein Diets ◽  
Effect Of Feeding

Abstract An experiment was conducted to evaluate the effect of feeding low crude protein (CP) diets and supplying 110% of the first 5 limiting AA on performance and diarrhea incidence in weaned pigs. One of 3 treatments were randomly assigned to 81 piglets (BW: 8.2 ± 1.2 kg; 9 pens/treatment; 3 pigs/pen) during 2 phases (3 wk each): a high CP (HP; 21 and 18%), a low CP (LP; 18 and 15%), or a low CP at 110% Lys, Met, Trp, Thr, Val requirements (LPAA; 18 and 15%) diet for phase 1 and 2, respectively. Pigs had ad libitum access to feed and water. Data were analyzed using PROC MIXED of SAS. During phase 1, pigs fed HP had the greatest ADG (P < 0.05), but ADFI and G:F were not different among treatments. In phase 2, no effect of treatments was observed on growth performance. Overall, pigs fed LPAA had the lowest (P < 0.05) ADG and G:F, and feeding LP decreased (P < 0.05) G:F. The ADFI was not affected by the treatments. Only during wk 3, pigs fed LPAA increased (P < 0.05) and LP tended to increase (P < 0.10) frequency of diarrhea compared to HP. On d 21, no treatment effect was observed for ileal villi height, but LPAA reduced (P < 0.05) and LP tended to reduce (P < 0.10) crypt depth compared to HP. Diet analysis revealed that LP was deficient in Ile and non-essential AA (NEAA), and LPAA contained inadequate levels of Ile, Leu, His and NEAA, which explains why reduced pig performance was achieved with these 2 diets. In conclusion, low CP diets, even with additional supplementation of the first 5 limiting AA, if deficient in the next limiting AA, results in negative effects on performance and diarrhea incidence.

214 Dietary Supplementation with a Combination of Valine and Isoleucine Annuls the Negative Effects of Very Low Protein Diets on Growth and Gut Development of Young Pigs

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 112-113
Author(s):  
Mohammad Habibi ◽  
Parniyan Goodarzi ◽  
Cedrick N N Shili ◽  
Julia Sutton ◽  
Caitlyn Wileman ◽  
...  
Keyword(s):  
Thermal Radiation ◽  
Growth Performance ◽  
Nitrogen Excretion ◽  
Gut Development ◽  
Negative Effects ◽  
Final Body Weight ◽  
Low Protein ◽  
Feed Cost ◽  
Young Pigs ◽  
Protein Diets

Abstract Increased feed cost, post-weaning diarrhea, and nitrogen excretion are challenging the modern swine industry. Very low-protein (LP) diets (>4% units reduced protein) may reduce feed cost, diarrhea and nitrogen excretion; however, these diets impair growth performance of pigs despite supplementation of limiting amino acids (i.e., lysine, threonine, methionine and tryptophan). The objective of this study was to investigate the effect of valine, isoleucine and combination of both supplemented to VLP diet on growth, thermal radiation, and gut development in young pigs. Forty three-week-old weaned barrows were weighed (6.75 ± 0.14 kg) and randomly assigned to five groups (8 pigs/group) including: 1) standard diet or positive control (PC), 2) LP diet, 3) LP + valine (LPV), 4) LP + isoleucine (LPI) and 5) LP + both valine and isoleucine (LPVI) for five weeks. Daily feed intake and weekly growth characteristics were measured and weekly thermal images were captured. All pigs were euthanized at week 5 and tissue samples collected. All data were analyzed with univariate GLM followed by Tukey’s post-hoc test (SPSS®). Compared to PC, final body weight, average daily gain, average daily protein intake and gain:feed ratio were reduced in LP group, but feeding pigs with LPVI partially or completely recovered these parameters. Pigs fed with LPVI had higher thermal radiation than those fed with PC, LP, and LPI on days 28 and 35 of study and had a greater the area under the curve for thermal radiation than LP and LPI. Duodenal villus width and crypt depth, and ileal villus height were decreased in LP relative to PC, but LPVI either partially or fully recovered these measurements. In conclusion, supplementing a combination of valine and isoleucine recovered the negative effects of very low-protein diets on growth performance and gut development, but increased the thermal radiation in weaned pigs.

scholarly journals 244 Effect of Fiber Source and Crude Protein Level on Nursery Pig Performance

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 94-95
Author(s):  
Kelsey Hammers ◽  
Hilda I Calderon ◽  
Mike D Tokach ◽  
Jason C Woodworth ◽  
Robert D Goodband ◽  
...  
Keyword(s):  
Growth Performance ◽  
Crude Protein ◽  
Phase 1 ◽  
Experimental Period ◽  
Oat Hulls ◽  
Nursery Pigs ◽  
Nursery Pig ◽  
Pig Performance ◽  
Two Phases ◽  
Main Effects

Abstract A total of 360 pigs (DNA 200′400, initially 5.0 kg) were used in a 45-d growth trial to determine the effects of fiber source and crude protein (CP) level in diets without pharmacological levels of ZnO on nursery pig growth performance and fecal dry matter (DM). Pigs were randomly assigned to 1 of 8 treatments with 5 pigs/pen and 9 pens/treatment. Treatments were arranged in a 2×4 factorial with main effects of CP (21 or 18%) and fiber source [none, coarse wheat bran (CWB), oat hulls, or cellulose (Arbocel, J. Rettenmaier USA, Schoolcraft, MI)]. Fiber source was added to equalize the level of insoluble fiber contributed from 4% CWB, resulting in the addition of 1.85% oat hulls or 1.55% cellulose. Diets were fed in two phases (d 0 to 10 and 10 to 24) followed by a common diet (d 24 to 45). The 21% CP diets contained 1.40% SID Lys in phase 1 and 1.35% SID Lys in phase 2. Treatment diets were formulated to a maximum SID Lys:digestible CP level of 6.35%, thus SID Lys decreased in the 18% CP (1.25% SID Lys) diets. Data were analyzed using the lmer function in R. No fiber source × CP level interactions (P >0.05) were observed. Decreasing dietary CP decreased (P = 0.05) ADG, G:F, and d 24 BW. Overall, ADG and d 45 BW decreased (P < 0.05) for pigs fed 18% CP diets. No main effects of fiber source were observed for growth performance throughout the study. Fecal DM increased (P < 0.05) for pigs fed added cellulose compared to pigs fed no fiber or CWB in the experimental period. In conclusion, reducing dietary CP decreased growth performance and the inclusion of cellulose improved fecal DM of nursery pigs.

scholarly journals Effects of Dietary Protein Levels on Bamei Pig Intestinal Colony Compositional Traits

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Dong Wang ◽  
Guoshun Chen ◽  
Lili Song ◽  
Mingjie Chai ◽  
Yongfeng Wang ◽  
...  
Keyword(s):  
Dietary Protein ◽  
Protein Level ◽  
Crude Protein ◽  
Microbial Flora ◽  
Protein Levels ◽  
Low Protein ◽  
Dietary Crude Protein ◽  
Protein Diets ◽  
Crude Protein Level ◽  
Microbial Diversities

Diets containing different crude protein levels (16%, 14%, and 12%) were created to feed Bamei pigs in order to study the effect of these compositions on intestinal colonies. Therefore, 27 healthy Bamei pigs of similar weight ( 20.99   kg ± 0.16   kg ) were selected and randomly divided into three groups for microbial diversity analysis. The results of this study show that microbial diversities and abundances in Bamei pig jejunum and caecum samples after feeding with different dietary protein levels were significantly different. Dietary crude protein level exerted no significant effect on the Shannon index for cecum microbes in these pigs, while Simpson, ACE, and Chao1 indices for group I were all significantly higher than those of either the control group or group II ( P < 0.05 ). Indeed, data show that microbial diversities and abundances in the 14% protein level group were higher than those in either the 16% or 12% groups. Dominant bacteria present in jejunum and cecum samples given low-protein diets were members of the phyla Firmicutes and Bacteroidetes. Data show that as dietary crude protein level decreases, representatives of the microbial flora genus Lactobacillus in jejunum and cecum samples gradually increases. Values for the KEGG functional prediction of microbial flora at different dietary protein levels also show that genes of jejunum and cecum microorganisms were mainly enriched in the “metabolism” pathway and indicate that low protein diets increase intestinal metabolic activity. Therefore, we recommend that Bamei pig dietary protein levels are reduced 2% from their existing level of 16% crude protein. We also suggest that essential synthetic amino acids (AA) are added to optimize this ideal protein model as this will increase intestinal flora diversity in these pigs and enhance health. These changes will have a positive effect in promoting the healthy growth of Bamei pigs.

Effect of antibiotic-free, low-protein diets with specific amino acid compositions on growth and intestinal flora in weaned pigs

2020 ◽  
Vol 11 (1) ◽  
pp. 493-507 ◽  
Author(s):  
Junyan Zhou ◽  
Yuming Wang ◽  
Xiangzhou Zeng ◽  
Tao Zhang ◽  
Peili Li ◽  
...  
Keyword(s):  
Amino Acid ◽  
Growth Performance ◽  
Intestinal Flora ◽  
Amino Acid Profile ◽  
Gut Health ◽  
Weaned Pigs ◽  
Specific Amino Acid ◽  
Amino Acid Compositions ◽  
Low Protein ◽  
Protein Diets

This study investigated the effects of modulation of the amino acid profile on growth performance and gut health in weaned pigs fed an antibiotic-free, low-protein diet.

Effects of low protein diets on growth performance, carcass traits and ammonia emission of barrows and gilts

2013 ◽  
Vol 53 (2) ◽  
pp. 146 ◽  
Author(s):  
J. Madrid ◽  
S. Martínez ◽  
C. López ◽  
J. Orengo ◽  
M. J. López ◽  
...  
Keyword(s):  
Growth Performance ◽  
Control Diet ◽  
Average Daily Gain ◽  
Carcass Traits ◽  
Backfat Thickness ◽  
Ammonia Emission ◽  
Growing Period ◽  
Low Protein ◽  
Pig Performance ◽  
Protein Diets

The aim of the present research was to determine the effect of low protein diets on growth performance, carcass traits and ammonia emission from barrows and gilts during the growing and finishing periods. For each period, three diets were formulated. A control diet (C) with 160 and 155 g crude protein (CP)/kg for the growing and finishing, respectively, a medium level diet (M) and a low level diet (L) with 10 and 20 g CP/kg less than the C, respectively. All the diets were supplemented with crystalline amino acids on an ideal protein basis. Barrows (90) and gilts (90) were sorted by bodyweight and sex and housed in three identical manually ventilated chambers. No significant effects of diet on pig performance were observed. In the finishing period, barrows were heavier (P < 0.001) and had higher average daily gain (P < 0.01) and average daily feed intake (P < 0.01) than gilts. Pigs fed the L diet had higher backfat thickness than those fed the other diets in the growing period (P < 0.05). Backfat thickness was higher (P < 0.01) in barrows than in gilts. Muscle depth decreased in pigs fed the L diet in the finishing period. Muscle depth was not affected (P > 0.05) by sex. Average ammonia emissions during the last 6 days were 117.7, 94.2 and 85.5 mg ammonia/kg pig.day for pigs fed the C, M and L diets, respectively. The results show that a reduction in dietary CP of 10 g/kg, accompanied by supplementation with crystalline amino acid reduced ammonia emission by 19.9% with no detrimental effects on growth performance and carcass characteristics, and a reduction of 20 g/kg reduced ammonia emission by 27.3% with no detrimental effects on growth performance but reduced muscle depth at slaughter.

scholarly journals Industrial amino acids in diets for piglets and growing pigs

1988 ◽  
Vol 60 (7) ◽  
pp. 673-683
Author(s):  
J. Inborr ◽  
K. Suomi
Keyword(s):  
Amino Acids ◽  
Health Status ◽  
Protein Content ◽  
Crude Protein ◽  
Control Diet ◽  
Growing Pigs ◽  
Protein Amino Acid ◽  
Low Protein ◽  
Slaughter Pigs ◽  
Protein Diets

Two production trials with piglets and one with slaughter pigs were carried out in order to investigate the effects of reducing the protein content in the diets followed by an addition of industrial amino acids on performance and health status. In the first piglet trial the crude protein content of the control diet was decreased from 20 to 18.3 % and in the second from 18.3 to 16.7 %. In the trial with growing pigs, the protein content of the control diet was decreased from 17.0 to 15.5 %. Industrial L-lysine, DL-methionine and L-threonine were added to the low protein diets to get the same levels of these amino acids as in the control diets. Piglet performance was similar on all treatments indicating equal availability of added and protein-bound amino acids. Health status of piglets on the low protein diets was considerably improved, indicating less predisposition to post weaning diarrhoea. Pigs on the low protein amino acid fortified diet tended to gain weight faster (788 vs. 743 g/day; p

scholarly journals Different Dietary Protein Sources in Low-Protein Diets Induces Changes in Antioxidant Capacity, Immunity, Fecal Microbiota and Metabolites of Weaned Piglets

2021 ◽  
Author(s):  
Lianhua Zhang ◽  
Xiangshu Piao
Keyword(s):  
Antioxidant Capacity ◽  
Soybean Meal ◽  
Phase 1 ◽  
Serum Levels ◽  
Fecal Microbiota ◽  
Phase 2 ◽  
Weaned Piglets ◽  
Low Protein ◽  
Dietary Treatments ◽  
Protein Diets

Abstract Background: The inclusion of high-quality proteins and the protein restriction are commonly used in swine production. Our study was conducted to evaluate the effects of hydrolyzed wheat protein (HWP), fermented soybean meal (FSBM), and enzyme-treated soybean meal (ESBM) in low-protein diets on antioxidant capacity, immunity, fecal microbiota and metabolites of weaned piglets.Methods: A total of 144 weaned piglets were randomly assigned to 3 dietary treatments with 6 replications of 8 piglets per pen. The experiment was divided into phase 1 ( days 0-14) and phase 2 (days 15-28). The dietary treatments contained 15.90% HWP, 15.80% FSBM, and 15.10% ESBM in phase 1, and 7.90% HWP, 7.80% FSBM, and 7.50% ESBM in phase 2, respectively.Results: The ADG of piglets in the ESBM group was higher (P < 0.05) than HWP and FSBM during days 1-28. Compared with HWP and FSBM, piglets in ESBM had higher (P < 0.05) serum levels of FRAP and SOD on days 14, as well as higher (P < 0.05) serum FRAP level on days 28. Piglets in ESBM had lower (P < 0.05) serum levels of DAO and IL-1β than HWP on day 28. ESBM significantly increased the abundance of Bacteroidetes, Oscillospiraceae and Christensenellaceae and decreased the Clostridiaceae abundance in the feces compared with HWP and FSBM. The PICRUSt analysis revealed that the number of gene tags involved in valine, leucine and isoleucine degradation and lysine degradation in ESBM were lower (P < 0.05) than HWP and FSBM. Piglets in ESBM had higher (P < 0.05) fecal butyrate content compared with FSBM, and ESBM tended to decrease (P = 0.076) fecal cadaverine level.Conclusions: Overall, ESBM had advantages over HWP and FSBM in improving antioxidant capacity, immunity, and fecal microbiota and metabolites of weaned piglets.

scholarly journals Low Crude Protein Diet Affects the Intestinal Microbiome and Metabolome Differently in Barrows and Gilts

2021 ◽  
Vol 12 ◽  
Author(s):  
Xin Tao ◽  
Bo Deng ◽  
Qizhi Yuan ◽  
Xiaoming Men ◽  
Jie Wu ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Dietary Protein ◽  
Crude Protein ◽  
High Protein Diet ◽  
Stage I ◽  
Protein Diet ◽  
Stage Ii ◽  
Protein Levels ◽  
Low Protein ◽  
Protein Diets

Low protein diets are commonly used in the growing-finishing pig stage of swine production; however, the effects of low dietary protein on the intestinal microbiota and their metabolites, and their association with pig sex, remain unclear. The present study aimed to assess the impact of a low crude protein (CP) diet on the gut microbiome and metabolome, and to reveal any relationship with sex. Barrows and gilts (both n = 24; initial body = 68.33 ± 0.881 kg) were allocated into two treatments according to sex. The four groups comprised two pairs of gilts and barrows fed with a high protein diet (CP 17% at stage I; CP 13% at stage II) and a low protein diet (CP 15% at stage I; CP 11% at stage II), respectively, for 51 d. Eight pigs in each group were slaughtered and their colon contents were collected. Intestinal microbiota and their metabolites were assessed using 16S rRNA sequencing and tandem mass spectrometry, respectively. The low protein diet increased intestinal microbiota species and richness indices (P &lt; 0.05) in both sexes compared with the high protein diet. The sample Shannon index was different (P &lt; 0.01) between barrows and gilts. At the genus level, unidentified Clostridiales (P &lt; 0.05), Neisseria (P &lt; 0.05), unidentified Prevotellaceae (P &lt; 0.01) and Gracilibacteria (P &lt; 0.05) were affected by dietary protein levels. The relative abundance of unidentified Prevotellaceae was different (P &lt; 0.01) between barrows and gilts. The influence of dietary protein levels on Neisseria (P &lt; 0.05), unidentified Prevotellaceae (P &lt; 0.01) and Gracilibacteria (P &lt; 0.05) were associated with sex. Metabolomic profiling indicated that dietary protein levels mainly affected intestinal metabolites in gilts rather than barrows. A total of 434 differentially abundant metabolites were identified in gilts fed the two protein diets. Correlation analysis identified that six differentially abundant microbiota communities were closely associated with twelve metabolites that were enriched for amino acids, inflammation, immune, and disease-related metabolic pathways. These results suggested that decreasing dietary protein contents changed the intestinal microbiota in growing-finishing pigs, which selectively affected the intestinal metabolite profiles in gilts.

scholarly journals Performance in blue fox (Vulpes lagopus) fed low-protein diets supplemented with DL-methionine and L-histidine

2018 ◽  
Vol 27 (3) ◽  
Author(s):  
Vappu Ylinen ◽  
Päivi Pylkkö ◽  
Jussi Peura ◽  
Essi Tuomola ◽  
Jarmo Valaja
Keyword(s):  
Protein Level ◽  
Crude Protein ◽  
Average Daily Gain ◽  
Vulpes Lagopus ◽  
Low Protein ◽  
Digestible Crude Protein ◽  
Blue Fox ◽  
Metabolisable Energy ◽  
Protein Diets ◽  
Daily Gain

The effects of low-protein diets supplemented with DL-methionine (MET) and L-histidine (HIS) on growth, pelt size and pelt quality were studied in two performance trials conducted at the Kannus Research Farm Luova Ltd, Finland. Both trials were conducted with 200 blue foxes, caged male-female pairs, initial age on average 20 weeks (trial 1) and 25 weeks (trial 2). In trial 1, diets contained digestible crude protein (DCP) 24%, 20% and 16% of metabolisable energy (ME). In trial 2, diets contained DCP 20%, 16.5% and 13% of ME. In both trials, the middle protein level was fed with or without MET and the lowest protein level was fed with MET and with or without HIS. In trial 1, blue foxes showed the greatest average daily gain (ADG) in the highest protein diet. Pelt size and pelt quality were not affected. In trial 2, blue foxes showed the greatest ADG in the low-protein groups. Pelt size and pelt quality were not affected.

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