PSXII-21 Pregnancy associated glycoproteins for pregnancy diagnosis on day 25 of gestation

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 422-422
Author(s):  
Matthew P Holton ◽  
Gabriela de Melo ◽  
Dylan B Davis ◽  
R Lawton Stewart ◽  
Ky G Pohler ◽  
...  
Keyword(s):  
Plasma Concentrations ◽  
False Negative ◽  
Area Under The Curve ◽  
Pairwise Comparison ◽  
Beef Cows ◽  
Fetal Mortality ◽  
Operating Characteristics ◽  
Gold Standard Method ◽  
Pregnancy Diagnosis ◽  
Sensitivity Specificity

Abstract The objective of this study was to evaluate the use of plasma concentrations of pregnancy associated glycoproteins (PAG) for pregnancy diagnosis in beef cows on day 25 of gestation. Additionally, differences in circulating concentrations of PAG among cows that experienced late embryonic/early fetal mortality (LEM) were evaluated. Beef cows (n = 214) were exposed to the 7-d CO-Synch + CIDR protocol followed by fixed-time artificial insemination (day 0). Blood samples were collected on days 25 and 29 to determine circulating concentrations of PAG and ultrasonography on day 29 was used as the gold-standard method for pregnancy diagnosis. On days 25 and 29, PAG concentrations were greater (P < 0.001) in pregnant compared with non-pregnant cows. Sensitivity, specificity, positive predicted value (PPV), negative predicted value (NPV), and accuracy on day 25 were 87, 80, 83, 84, and 83%, respectively. Sensitivity, specificity, PPV, NPV, and accuracy for PAG on day 29 were 99, 94, 96, 97, and 99%, respectively. Pairwise comparison of receiver operating characteristics curve analysis indicated greater area under the curve for PAG on day 29 compared with day 25 (P < 0.001). There were no differences in plasma concentrations of PAG on day 25 among cows that experienced LEM compared to cows that maintained pregnancy (P = 0.46). Concentrations of PAG on day 29 were reduced (P = 0.03) in cows that experienced LEM compared with cows that maintained pregnancy, and were not different between LEM and non-pregnant cows (P = 0.12). In summary, cows that experienced LEM had decreased plasma concentrations of PAG compared with cows that maintained pregnancy. The PAG assay utilized in this study yielded accurate pregnancy diagnosis results on day 29 of gestation: however, the presence of false negative results (NPV = 84%) limits its use on day 25 of gestation.

238 Luteal Color Doppler Ultrasonography for Early Pregnancy Diagnosis in Postpartum Bos Taurus Beef Cows

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 121-122
Author(s):  
Matthew P Holton ◽  
Nicky Oosthuizen ◽  
Gabriela de Melo ◽  
Dylan B Davis ◽  
R Lawton Stewart ◽  
...  
Keyword(s):  
Early Pregnancy ◽  
Doppler Ultrasonography ◽  
Bos Taurus ◽  
Color Doppler ◽  
Blood Perfusion ◽  
Beef Cows ◽  
Color Doppler Ultrasonography ◽  
Fetal Mortality ◽  
Pregnancy Diagnosis ◽  
Sensitivity Specificity

Abstract The objective of this study was to evaluate the use of corpus luteum (CL) color doppler ultrasonography (CD) for early pregnancy diagnosis in Bos taurus beef cows and evaluate differences in CL blood perfusion among cows that experience late embryonic/early fetal mortality (LEM). Postpartum Bos taurus beef cows (n = 214) from three locations were exposed to a 7-d CO-Synch + CIDR protocol followed by fixed-time artificial insemination (day 0). On days 20 and 22, B-mode and CD ultrasonography were performed to evaluate CL morphometries and blood perfusion, respectively. Cows were considered non-pregnant when CL area was < 20 mm2 or estimated blood perfusion was ≤ 25%. Conventional ultrasonography on day 29 was utilized as the gold standard method. Pregnant cows had greater CL diameter, CL area, and CL volume when compared to non-pregnant cows on day 20 and 22 (P < 0.001; Table 1). Additionally, percentage of central, peripheral, and total luteal blood perfusion, as well as the respective blood perfusion scores were greater (P < 0.001) in pregnant compared with non-pregnant cows on both day 20 and 22. Sensitivity, specificity, positive predicted value (PPV), negative predicted value (NPV), and accuracy for the CD on day 20 were 100, 74, 81, 100, and 88%, respectively. Sensitivity, specificity, PPV, NPV, and accuracy for CD on day 22 were 100, 86, 89, 100, and 93%, respectively. Pairwise comparison of receiver operating characteristics curve analysis indicated no differences between CD on days 20 and 22 (P = 0.12). No differences in luteal blood perfusion were observed on day 20 or 22 among cows that maintained pregnancies compared with LEM cows (P ≥ 0.27). In conclusion, CD successfully detected most non-pregnant cows (81 and 89% on day 20 and 22, respectively) and false negative results were absent (NPV = 100%).

scholarly journals New screening approach to detecting congenital syphilis in China: a retrospective cohort study

2020 ◽  
pp. archdischild-2020-320549
Author(s):  
Fang Hu ◽  
Shuai-Jun Guo ◽  
Jian-Jun Lu ◽  
Ning-Xuan Hua ◽  
Yan-Yan Song ◽  
...  
Keyword(s):  
Predictive Value ◽  
Congenital Syphilis ◽  
Area Under The Curve ◽  
Diagnostic Tools ◽  
Operating Characteristics ◽  
Loss To Follow Up ◽  
Sensitivity Specificity ◽  
Mother To Child ◽  
Rule Out

BackgroundDiagnosis of congenital syphilis (CS) is not straightforward and can be challenging. This study aimed to evaluate the validity of an algorithm using timing of maternal antisyphilis treatment and titres of non-treponemal antibody as predictors of CS.MethodsConfirmed CS cases and those where CS was excluded were obtained from the Guangzhou Prevention of Mother-to-Child Transmission of syphilis programme between 2011 and 2019. We calculated sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) using receiver operating characteristics (ROC) in two situations: (1) receiving antisyphilis treatment or no-treatment during pregnancy and (2) initiating treatment before 28 gestational weeks (GWs), initiating after 28 GWs or receiving no treatment for syphilis seropositive women.ResultsAmong 1558 syphilis-exposed children, 39 had confirmed CS. Area under the curve, sensitivity and specificity of maternal non-treponemal titres before treatment and treatment during pregnancy were 0.80, 76.9%, 78.7% and 0.79, 69.2%, 88.7%, respectively, for children with CS. For the algorithm, ROC results showed that PPV and NPV for predicting CS were 37.3% and 96.4% (non-treponemal titres cut-off value 1:8 and no antisyphilis treatment), 9.4% and 100% (non-treponemal titres cut-off value 1:16 and treatment after 28 GWs), 4.2% and 99.5% (non-treponemal titres cut-off value 1:32 and treatment before 28 GWs), respectively.ConclusionsAn algorithm using maternal non-treponemal titres and timing of treatment during pregnancy could be an effective strategy to diagnose or rule out CS, especially when the rate of loss to follow-up is high or there are no straightforward diagnostic tools.

scholarly journals The role of red cell distribution width in the differential diagnosis of iron deficiency anemia and non-transfusion dependent thalassemia patients

2018 ◽  
Vol 10 (3) ◽  
Author(s):  
Pokpong Piriyakhuntorn ◽  
Adisak Tantiworawit ◽  
Thanawat Rattanathammethee ◽  
Chatree Chai-Adisaksopha ◽  
Ekarat Rattarittamrong ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Predictive Value ◽  
Area Under The Curve ◽  
Microcytic Anemia ◽  
Deficiency Anemia ◽  
Operating Characteristics ◽  
Training Set ◽  
Distribution Width ◽  
Validation Set ◽  
Sensitivity Specificity

This study aims to find the cut-off value and diagnostic accuracy of the use of RDW as initial investigation in enabling the differentiation between IDA and NTDT patients. Patients with microcytic anemia were enrolled in the training set and used to plot a receiving operating characteristics (ROC) curve to obtain the cut-off value of RDW. A second set of patients were included in the validation set and used to analyze the diagnostic accuracy. We recruited 94 IDA and 64 NTDT patients into the training set. The area under the curve of the ROC in the training set was 0.803. The best cut-off value of RDW in the diagnosis of NTDT was 21.0% with a sensitivity and specificity of 81.3% and 55.3% respectively. In the validation set, there were 34 IDA and 58 NTDT patients using the cut-off value of >21.0% to validate. The sensitivity, specificity, positive predictive value and negative predictive value were 84.5%, 70.6%, 83.1% and 72.7% respectively. We can therefore conclude that RDW >21.0% is useful in differentiating between IDA and NTDT patients with high diagnostic accuracy

scholarly journals Evaluation of cerebral glioma using 3T diffusion kurtosis tensor imaging and the relationship between diffusion kurtosis metrics and tumor cellularity

2017 ◽  
Vol 45 (4) ◽  
pp. 1347-1358 ◽  
Author(s):  
Chong Qi ◽  
Song Yang ◽  
Lanxi Meng ◽  
Huiyuan Chen ◽  
Zhenlan Li ◽  
...  
Keyword(s):  
Mean Diffusivity ◽  
Area Under The Curve ◽  
Tumor Grade ◽  
Superior Performance ◽  
Cerebral Glioma ◽  
Operating Characteristics ◽  
Significant Difference ◽  
Characteristics Analysis ◽  
Sensitivity Specificity ◽  
Diffusion Kurtosis

Purpose To evaluate the clinical utility of diffusion kurtosis tensor imaging in the characterization of cerebral glioma and investigate correlations between diffusion and kurtosis metrics with tumor cellularity. Materials and Methods A group of 163 patients (age: 40.5 ± 11.5 years) diagnosed with cerebral glioma underwent diffusion kurtosis tensor imaging with a 3 T scanner. Diffusion and kurtosis metrics were measured in the solid part of tumors, and their abilities to distinguish between tumor grades was evaluated. In addition, we analyzed correlations between the metrics and tumor cellularity. Results Mean kurtosis (MK) revealed a significant difference between each pair of tumor grades ( P < 0.05) and produced the best performance in a receiver operating characteristics analysis (area under the curve [AUC] = 0.89, sensitivity/specificity = 83.3/90). In contrast, mean diffusivity (MD) revealed a significant difference only for tumor grade II versus IV ( P < 0.05). No significant differences between grades were detected with fractional anisotropy (FA; P > 0.05). Thus, kurtosis metrics exhibited a positive and strong correlation with tumor cellularity, while MD exhibited a negative or weak correlation with tumor cellularity. Conclusion Diffusion kurtosis metrics, particularly MK, demonstrated superior performance in distinguishing cerebral glioma of different grades compared with conventional diffusion metrics, and were closely associated with tumor cellularity.

Deep-learning for predicting C-shaped canals in mandibular second molars on panoramic radiographs

2021 ◽  
pp. 20200513
Author(s):  
Su-Jin Jeon ◽  
Jong-Pil Yun ◽  
Han-Gyeol Yeom ◽  
Woo-Sang Shin ◽  
Jong-Hyun Lee ◽  
...  
Keyword(s):  
Deep Learning ◽  
Cone Beam Ct ◽  
Area Under The Curve ◽  
Roc Curves ◽  
Learning System ◽  
Operating Characteristics ◽  
Panoramic Radiographs ◽  
Test Sets ◽  
Sensitivity Specificity ◽  
Deep Learning Model

Objective: The aim of this study was to evaluate the use of a convolutional neural network (CNN) system for predicting C-shaped canals in mandibular second molars on panoramic radiographs. Methods: Panoramic and cone beam CT (CBCT) images obtained from June 2018 to May 2020 were screened and 1020 patients were selected. Our dataset of 2040 sound mandibular second molars comprised 887 C-shaped canals and 1153 non-C-shaped canals. To confirm the presence of a C-shaped canal, CBCT images were analyzed by a radiologist and set as the gold standard. A CNN-based deep-learning model for predicting C-shaped canals was built using Xception. The training and test sets were set to 80 to 20%, respectively. Diagnostic performance was evaluated using accuracy, sensitivity, specificity, and precision. Receiver-operating characteristics (ROC) curves were drawn, and the area under the curve (AUC) values were calculated. Further, gradient-weighted class activation maps (Grad-CAM) were generated to localize the anatomy that contributed to the predictions. Results: The accuracy, sensitivity, specificity, and precision of the CNN model were 95.1, 92.7, 97.0, and 95.9%, respectively. Grad-CAM analysis showed that the CNN model mainly identified root canal shapes converging into the apex to predict the C-shaped canals, while the root furcation was predominantly used for predicting the non-C-shaped canals. Conclusions: The deep-learning system had significant accuracy in predicting C-shaped canals of mandibular second molars on panoramic radiographs.

Gastric cancer detection using the serum pepsinogen test method

2021 ◽  
pp. 030089162110149
Author(s):  
Dragan Trivanovic ◽  
Stjepko Plestina ◽  
Lorena Honovic ◽  
Renata Dobrila-Dintinjana ◽  
Jelena Vlasic Tanaskovic ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Early Detection ◽  
Predictive Value ◽  
Area Under The Curve ◽  
Test Method ◽  
Operating Characteristics ◽  
Roc Curve Analysis ◽  
Laboratory Assessment ◽  
Pepsinogen I ◽  
Sensitivity Specificity

Background: Gastric cancer (GC) is the eighth most common cause of cancer deaths in Croatia and one of the most common causes of cancer deaths worldwide. A reliable diagnostic tool for the early detection of GC is essential. Objective: We previously suggested a pepsinogen test method to reduce the mortality from GC by allowing early detection. Here, we report an updated analysis from a prospective single-center clinical study to evaluate the sensitivity and specificity of the pepsinogen test method and to determine whether this test can be used as a part of routine laboratory assessment of high-risk patients. Methods: We present mature data of the pepsinogen test method in the Croatian population after a median follow-up of 36 months. Statistical analyses were performed using a Mann-Whitney U test, multiple logistic regression, and receiver operating characteristics (ROC) to evaluate the predictive power of the assayed biomarkers. Results: Of the 116 patients, 25 patients had GC and 91 demonstrated a nonmalignant pathology based on tissue biopsy. Cutoff values were pepsinogen I ⩽70 and pepsinogen I/II ratio ⩽3.0. Using ROC curve analysis, the accuracy, sensitivity, specificity, positive predictive value, and negative predictive value were determined to be 87.22%, 78.12%, 90.10%, 71.43%, and 92.86%, respectively, for the diagnosis of GC. The area under the curve was 0.700 (95% confidence interval 0.57–0.83). Conclusion: Pepsinogen tests are valuable for screening a population in need of further diagnosis and could help to avoid unnecessary invasive endoscopic procedures.

Copeptin and NT-proBNP for prediction of all-cause and cardiovascular death in ischemic stroke

Neurology ◽  
2017 ◽  
Vol 88 (20) ◽  
pp. 1899-1905 ◽  
Author(s):  
Wen-Jun Tu ◽  
Guo-Zhao Ma ◽  
Ying Ni ◽  
Xia-Sheng Hu ◽  
Ding-Zhen Luo ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Plasma Concentrations ◽  
Area Under The Curve ◽  
Cardiovascular Death ◽  
Chinese Patients ◽  
Operating Characteristics ◽  
Multicenter Observational Study ◽  
All Cause Mortality ◽  
Fourth Quartile ◽  
Cvd Mortality

Objective:To evaluate long-term mortality in patients with acute ischemic stroke (AIS) by exploring the correlation between death and plasma concentrations of copeptin and N-terminal pro-B-type natriuretic peptide (NT-proBNP) in a cohort study.Methods:In a prospective, multicenter observational study of 4,215 patients with AIS, copeptin and NT-proBNP levels were measured with a standardized method when patients were admitted to hospital. The primary endpoint was all-cause mortality or cardiovascular disease (CVD) mortality within 1 year.Results:During a follow-up period, 906 patients (20.1%, 95% confidence interval [CI] 18.9–21.2) died, including 589 cases of CVD mortality (13.1%, 95% CI 12.1–14.0). With the use of a multivariate analysis, both markers were found to have prognostic value in the same model (CVD mortality: odds ratio [OR] for fourth quartile of copeptin and NT-proBNP 1.68 and 2.58, 95% CI 1.22–2.49 and 1.76–4.05, respectively; all-cause mortality: OR for fourth quartile of copeptin and NT-proBNP 1.48 and 2.47, 95% CI 1.22–2.03 and 1.68–3.95, respectively). In a receiver operating characteristics analysis of CVD mortality, the area under the curve varied from 0.80 to 0.83 (95% CI 0.79–0.87) when the index of NT-proBNP was added and increased to 0.86 (95% CI 0.83–0.90) when both markers were added.Conclusions:Copeptin and NT-proBNP may be useful independent prognostic markers of all-cause or CVD mortality in Chinese patients with AIS.

Evaluation of the Cardiac reader® as a point-of-care instrument for measurement of fibrin D-dimers in dogs

2009 ◽  
Vol 37 (05) ◽  
pp. 319-325 ◽  
Author(s):  
A. Moritz ◽  
N. Bauer
Keyword(s):  
Point Of Care ◽  
Plasma Concentrations ◽  
False Negative ◽  
Reference Interval ◽  
Comparison Method ◽  
Likelihood Ratios ◽  
Negative Results ◽  
Blood Specimens ◽  
Sensitivity Specificity ◽  
D Dimer

Summary Objective: The aim of this study was the evaluationof the automated point-of-care analyzer Cardiac reader® for quantitative fibrin D-dimer measurement compared to the Liatest™ D-Di™ run on the bench top analyzer STA Compact (all Roche). Material and methods: 41 fresh canine blood specimens submitted from the Clinic for Small Animals, Justus-Liebig-University Giessen were included. Measurement of fibrin D-dimers with the Liatest™ D-Di™ immunoturbidimetric assay was performed in citrated blood plasma stored at –80 °C until analysis. Using the Cardiac reader, D-dimer plasma concentrations were measured with an immunological assay in heparinized fresh (< 1 hour) whole blood specimens. Results: Mean intra-assay coefficient of variation (CV) was 6.13% (range: 2.2–10.0%) for the Liatest™ D-Di and 19.8% (range: 20.1–19.6%) for the Cardiac reader. Fibrin D-dimers detected with the STA Compact exceeded the upper limit of the reference interval (0.4 μg/ml; n = 62 healthy dogs) in 20/41 samples. There was a fair correlation between D-dimer measurements with the Cardiac reader and the comparison method (r = 0.78). Generally, results were underestimated with a mean proportional bias (0.45 μg/ml). The area under the Receiver Operating Characteristic (ROC) curve was 0.895 regarding the detection of increased fibrin D-dimers with the Cardiac reader. At a cut-off value of > 0.2 μg/ml, sensitivity/specificity were 85%/91% and positive/negative likelihood ratios were 8.93/0.17. At a lower cut-off value of 0.15 μg/ml sensitivity and specificity were 90% and 66%, respectively. Conclusion and clinical relevance: The Cardiac reader can be used for quantitative measurement of canine D-dimers, however, due to the odds of false negative results and the high imprecision the comparison method should be preferred.

The First Step in the Validation of a new Screen for Violence Risk in Acute Psychiatry: The Inpatient Context

2010 ◽  
Vol 26 (2) ◽  
pp. 92-99 ◽  
Author(s):  
P. Hartvig ◽  
J.O. Roaldset ◽  
T.A. Moger ◽  
B. Østberg ◽  
S. Bjørkly
Keyword(s):  
Risk Assessment ◽  
Area Under The Curve ◽  
Assessment Tools ◽  
Violence Risk ◽  
Operating Characteristics ◽  
Predictive Values ◽  
General Psychiatry ◽  
Psychiatric Units ◽  
One Year ◽  
Sensitivity Specificity

AbstractBackgroundInstruments for evaluating the risk of violence towards others have mostly been developed for assessment of risk for recidivism into violent crime in forensic psychiatry. In general psychiatry there is a considerable need for specialised, brief and structured assessment tools to inform risk decisions.MethodThe study aimed to validate a brief structured clinical risk assessment screen of inpatient violence (V-RISK-10), a 10-item structured clinical checklist with a good vignette-based interrater reliability (ICC=0.87). In this study it was used for risk assessment of a one-year sample of patients (N = 1.017) admitted to two acute psychiatric units. Risk assessments at admission were compared to prospective records of aggressive and violent acts during the hospital stay.ResultsResults showed a base rate for aggression of 9%. The predictive validity of the V-RISK-10 was estimated by Receiver Operating Characteristics (ROC). It yielded an area under the curve (AUC) of 0.83, with sensitivity/specificity of 0.81/0.73 and corresponding positive and negative predictive values (PPV/NPV) of 0.24/0.97. The screen was easy-to-use and showed a short completion time.ConclusionDespite promising results further validation studies are required before the V-RISK-10 is adopted into routine clinical practise.

scholarly journals May Antitransglutaminase Levels Predict Severity of Duodenal Lesions in Adults with Celiac Disease?

Medicina ◽  
2021 ◽  
Vol 57 (11) ◽  
pp. 1212
Author(s):  
Giuseppe Losurdo ◽  
Milena Di Leo ◽  
Edoardo Santamato ◽  
Antonio Giangaspero ◽  
Maria Rendina ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Diagnostic Performance ◽  
Villous Atrophy ◽  
Area Under The Curve ◽  
Curve Analysis ◽  
Operating Characteristics ◽  
Roc Curve Analysis ◽  
Upper Limit ◽  
Duodenal Lesions ◽  
Sensitivity Specificity

Background and Objective: Pediatric guidelines on celiac disease (CD) state that children with anti-transglutaminase antibodies (TGAs) >×10 upper limit of normal (ULN) may avoid endoscopy and biopsy. We aimed to evaluate whether these criteria may be suitable for villous atrophy diagnosis in CD adults. Materials and Methods: We retrospectively enrolled patients with CD aged >18 years. TGAs were expressed as xULN. Duodenal lesions were classified as atrophic or non-atrophic according to Marsh-Oberhuber. Fisher’s exact and t-test were used for variables comparison. Receiver operating characteristics (ROC) curve analysis was performed with estimation of area under the curve (AUC), sensitivity, specificity, and positive and negative predictive value (PPV/NPV). Results: One hundred and twenty-one patients were recruited. Sixty patients (49.6%) had TGA >×10 ULN, and 93 (76.8%) had villous atrophy. The cut-off of >×10 ULN had sensitivity = 53.7%, specificity = 64.3%, PPV = 83.3%, and NPV = 29.5% to predict atrophy. Therefore, considering pediatric criteria, in 50 (41.3%) patients, biopsy could have been avoided. Patient subgroup with atrophy had higher TGA levels despite being not significant (37.2 ± 15.3 vs. 8.0 ± 1.3 ULN, p = 0.06). In adults, a slightly better diagnostic performance was obtained using a cut-off of TGA >×6.2 ULN (sensitivity = 57.1%, specificity = 65.6%, and AUC = 0.62). Conclusions: Despite our confirmation that villous atrophy is linked to high TGA levels, CD and atrophy diagnosis based only on serology is not reliable in adults.

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