scholarly journals 131 Effects of Bacillus spp. probiotics on systemic immunity and intestinal health of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 74-75
Author(s):  
Yijie He ◽  
Kwangwook Kim ◽  
Cynthia Jinno ◽  
Zhaohai Wu ◽  
Rose Whelan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Control Diet ◽  
Enterotoxigenic Escherichia Coli ◽  
Jejunal Mucosa ◽  
Post Inoculation ◽  
Intestinal Health ◽  
Weaned Pigs ◽  
Systemic Immunity ◽  
E Coli ◽  
Bacillus Spp

Abstract The experiment was conducted to investigate the effects of Bacillus spp. on systemic immunity and intestinal health of weaned pigs infected with F18 E. coli. Weaned pigs (n = 36, 7.61 ± 0.40 kg BW) were randomly allotted to one of three treatments: a control diet and two diets supplemented with 500 mg/kg of Bacillus spp. strain 1 (PRO1) or strain 2 (PRO2). The experiment was conducted for 28 d, including 7 d before and 21 d after the first E. coli inoculation (d 0). The doses of F18 E. coli inoculum were 1010 cfu/3 mL oral dose daily for 3 consecutive days. Serum samples were collected on d 0 before inoculation, and d 3, 7, 14, and 21 post-inoculation (PI) to measure inflammatory meditators. All pigs were euthanized on d 21 PI to collect tissue samples for gut morphology, bacterial translocation, and gene expression analysis. Data were analyzed using the Mixed Procedure of SAS. Pigs in PRO1 had reduced (P < 0.05) serum haptoglobin on d 3 and d 6 PI compared with pigs in control. Supplementation of PRO1 or PRO2 reduced (P < 0.05) total coliforms in mesenteric lymph nodes on d 21 PI. Pigs in PRO1 had greater (P < 0.05) ileal villi height than pigs in control. Pigs in PRO2 had greater (P < 0.05) sulfomucin percentage in duodenal villi and greater (P < 0.05) sialomucin percentage in jejunal villi than pigs in control. Supplementation of PRO1 also up-regulated (P < 0.05) MUC2 gene expression in jejunal mucosa but reduced (P < 0.05) COX2 and IL1B gene expression in ileal mucosa on d 21 PI, compared with control. In conclusion, supplementation of Bacillus spp. may reduce systemic inflammation and enhance intestinal health of weaned pigs infected with F18 E. coli.

scholarly journals Supplementation of oligosaccharide-based polymer enhanced growth and disease resistance of weaned pigs by modulating intestinal integrity and systemic immunity

2022 ◽  
Vol 13 (1) ◽  
Author(s):  
Kwangwook Kim ◽  
Yijie He ◽  
Cynthia Jinno ◽  
Lauren Kovanda ◽  
Xunde Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Performance ◽  
Animal Health ◽  
Control Group ◽  
Jejunal Mucosa ◽  
Post Inoculation ◽  
Weaned Pigs ◽  
Ileal Mucosa ◽  
Total Coliforms ◽  
Systemic Immunity

Abstract Background There is a great demand for antibiotic alternatives to maintain animal health and productivity. The objective of this experiment was to determine the efficacy of dietary supplementation of a blood group A6 type 1 antigen oligosaccharides-based polymer (Coligo) on growth performance, diarrhea severity, intestinal health, and systemic immunity of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli (ETEC), when compared with antibiotics. Results Pigs in antibiotic carbadox or Coligo treatment groups had greater (P < 0.05) body weight on d 5 or d 11 post-inoculation (PI) than pigs in the control group, respectively. Supplementation of antibiotics or Coligo enhanced (P < 0.05) feed efficiency from d 0 to 5 PI and reduced (P < 0.05) frequency of diarrhea throughout the experiment, compared with pigs in the control group. Supplementation of antibiotics reduced (P < 0.05) fecal β-hemolytic coliforms on d 2, 5, and 8 PI. Pigs in antibiotics or Coligo groups had reduced (P < 0.05) neutrophil counts and serum haptoglobin concentration compared to pigs in the control group on d 2 and 5 PI. Pigs in Coligo had reduced (P < 0.05) total coliforms in mesenteric lymph nodes on d 5 and 11 PI, whereas pigs in antibiotics or Coligo groups had reduced (P < 0.05) total coliforms in spleen on d 11 PI compared with pigs in the control group. On d 5 PI, pigs in the Coligo group had greater (P < 0.05) gene expression of ZO1 in jejunal mucosa, but less (P < 0.05) mRNA expression of IL1B, IL6, and TNF in ileal mucosa, in comparison with pigs in the control group. Supplementation of antibiotics enhanced (P < 0.05) the gene expression of OCLN in jejunal mucosa but decreased (P < 0.05) IL1B and IL6 gene expression in ileal mucosa, compared with the control. On d 11 PI, supplementation of antibiotics or Coligo up-regulated (P < 0.05) gene expression of CLDN1 in jejunal mucosa, but Coligo reduced (P < 0.05) IL6 gene expression in ileal mucosa compared to pigs in the control group. Conclusions Supplementation of Coligo improved growth performance, alleviated diarrhea severity, and enhanced gut health in weaned pigs infected with ETEC F18 in a manner similar to in-feed antibiotics.

scholarly journals PSIV-11 Effects of very low-dose antibiotics on gene expression profiles in ileal mucosa of weaned pigs infected with a pathogenic E. coli

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 286-286
Author(s):  
Kwangwook Kim ◽  
Sungbong Jang ◽  
Yanhong Liu
Keyword(s):  
Gene Expression ◽  
Systemic Inflammation ◽  
Low Dose ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Post Inoculation ◽  
Growth Promoter ◽  
Weaned Pigs ◽  
Ileal Mucosa ◽  
E Coli

Abstract Our previous studies have shown that supplementation of low-dose antibiotic growth promoter (AGP) exacerbated growth performance and systemic inflammation of weaned pigs infected with pathogenic Escherichia coli (E. coli). The objective of this experiment, which is extension of our previous report, was to investigate the effect of low-dose AGP on gene expression in ileal mucosa of weaned pigs experimentally infected with F18 E. coli. Thirty-four pigs (6.88 ± 1.03 kg BW) were individually housed in disease containment rooms and randomly allotted to one of three treatments (9 to 13 pigs/treatment). The three dietary treatments were control diet (control), and 2 additional diets supplemented with 0.5 or 50 mg/kg of AGP (carbadox), respectively. The experiment lasted 18 d [7 d before and 11 d after first inoculation (d 0)]. The F18 E. coli inoculum was orally provided to all pigs with the dose of 1010 cfu/3 mL for 3 consecutive days. Total RNA [4 to 6 pigs/treatment on d 5; 5 to 7 pigs/treatment on 11 post-inoculation (PI)] was extracted from ileal mucosa to analyze gene expression profiles by Batch-Tag-Seq. The modulated differential gene expression were defined by 1.5-fold difference and a cutoff of P &lt; 0.05 using limma-voom package. All processed data were statistically analyzed and evaluated by PANTHER classification system to determine the biological process function of genes in these lists. Compared to control, supplementation of recommended-dose AGP down-regulated genes related to inflammatory responses on d 5 and 11 PI; whereas, feeding low-dose AGP up-regulated genes associated with negative regulation of metabolic process on d 5, but down-regulated the genes related to immune responses on d 11 PI. The present observations support adverse effects of low-dose AGP in our previous study, indicated by exacerbated the detrimental effects of E. coli infection on pigs’ growth rate, diarrhea and systemic inflammation.

478 Dietary Butyrate and Valerate Glycerides: Effects on Growth and Systemic Immunity of Weanling Piglets Coinfected with F4 and F18 Escherichia coli

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 211-212
Author(s):  
Lauren L Kovanda ◽  
Jungjae Park ◽  
Yijie He ◽  
Sangwoo Park ◽  
Ruochen Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Block Design ◽  
Enterotoxigenic Escherichia Coli ◽  
Post Inoculation ◽  
Weaned Pigs ◽  
Fecal Shedding ◽  
Systemic Immunity ◽  
Randomized Complete Block Design ◽  
Potential Benefits ◽  
Weanling Piglets

Abstract Enterotoxigenic Escherichia coli (ETEC) F4 and F18 are the two most dominant pathogenic strains in weaned pigs. The objective of this experiment was to test the effects of dietary monobutyrin and monovalerin on performance and systemic immunity of weanling piglets coinfected with F4/F18 ETEC. Twenty weaned pigs (8.21 ± 1.23 kg) were individually housed and were randomly allotted to one of three diets: control (n = 6), 0.1% monobutyrin (n = 7), or 0.1% monovalerin (n = 7). The experiment was conducted 14 days, including 7 days’ adaption and 7 days post-inoculation (PI). On d 0, d 1, and d 2 PI, pigs were inoculated with 0.5 × 109 CFU/1.5 mL each of F4 and F18 ETEC for three consecutive days. Diarrhea score was recorded daily to determine frequency of diarrhea. Piglets and feeders were weighed throughout the trial to analyze growth performance. Fecal cultures from pigs on d 0, 2, and 4 PI were inspected to identify the absence or presence of hemolytic coliforms. Blood was collected on d 0, 4, and 7 PI for complete blood cells count. All data were analyzed by the Proc Mixed of SAS with randomized complete block design. Pigs supplemented with monovalerin and monobutyrin had numerically higher ADG (249 and 282 g/day) from d 0 to d 7 PI than pigs in control (198 g/day). Supplementation of monovalerin reduced (P &lt; 0.05) frequency of diarrhea throughout the experiment. Pigs fed monovalerin had lower (P &lt; 0.05) neutrophil counts on d 4 PI compared with control. Hemolytic coliforms were observed in all fecal cultures from d 2 and d 4 PI, confirming fecal shedding of ETEC. Results of this study indicate the potential benefits of monovalerin supplementation on performance and disease resistance of weaned pigs coinfected with F4 and F8 ETEC.

scholarly journals Dietary Bacillus spp. enhanced growth and disease resistance of weaned pigs by modulating intestinal microbiota and systemic immunity

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Yijie He ◽  
Cynthia Jinno ◽  
Kwangwook Kim ◽  
Zhaohai Wu ◽  
Bie Tan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bacillus Subtilis ◽  
Growth Performance ◽  
Systemic Inflammation ◽  
Bacillus Pumilus ◽  
Jejunal Mucosa ◽  
Gut Health ◽  
Weaned Pigs ◽  
Ileal Mucosa ◽  
Systemic Immunity

Abstract Background Previous research has shown that dietary supplementation of Bacillus spp. probiotics exerts beneficial effects on animals’ growth. However, limited studies have evaluated the efficacy of Bacillus spp. on weaned pigs and their effects on host gut health and microbiome, and systemic immunity using a disease challenge model. The objective of this experiment was to investigate the effects of two Bacillus spp. strains (Bacillus subtilis DSM 32540 and Bacillus pumilus DSM 32539) on growth performance, diarrhea, intestinal health, microbiome, and systemic immunity of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli (ETEC). Results Pigs in PRO1 (Bacillus subtilis DSM 32540) had greater (P < 0.05) body weight on d 7 and 14 PI, greater (P < 0.05) ADG from d 0 to 7 and d 7 to 14 PI, compared with pigs in CON (Control). Pigs in PRO1 had milder (P < 0.05) diarrhea on d 2 and 3 PI compared with pigs in CON. However, no differences were observed in growth performance and diarrhea score between PRO2 (Bacillus pumilus DSM 32539) and CON groups. Supplementation of PRO1 decreased (P < 0.05) lymphocyte counts on d 7 and 14 PI, compared with CON. Supplementation of PRO1 and PRO2 both reduced (P < 0.05) total coliforms in mesenteric lymph nodes on d 21 PI. Pigs in PRO2 had greater (P < 0.05) goblet cell number and sulfomucin percentage in duodenal villi and greater (P < 0.05) sialomucin percentage in jejunal villi than pigs in CON. Supplementation of PRO1 up-regulated (P < 0.05) MUC2 gene expression in jejunal mucosa and reduced (P < 0.05) PTGS-2 and IL1B gene expression in ileal mucosa on d 21 PI, compared with CON. Pigs in PRO1 had reduced (P < 0.05) relative abundance of families Lachnospiraceae, Peptostreptococcaceae and Pasteurellaceae in the ileum. Conclusions Supplementation of Bacillus subtilis DSM 32540 improved growth performance, alleviated diarrhea severity, enhanced gut health, and reduced systemic inflammation of weaned pigs infected with ETEC F18. Although Bacillus pumilus DSM 32539 was able to alleviate systemic inflammation, it had limited impacts on growth performance and severity of diarrhea of ETEC F18 challenged weaned pigs.

scholarly journals 140 Effects of Bacillus subtilis probiotics on growth performance, diarrhea, and fecal β-hemolytic coliforms of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 79-80
Author(s):  
Yijie He ◽  
Kwangwook Kim ◽  
Cynthia Jinno ◽  
Lauren Kovanda ◽  
Seijoo Yang ◽  
...  
Keyword(s):  
Body Weight ◽  
Bacillus Subtilis ◽  
Growth Performance ◽  
Feed Efficiency ◽  
Control Diet ◽  
Positive Control ◽  
Negative Control ◽  
Enterotoxigenic Escherichia Coli ◽  
Weaned Pigs ◽  
E Coli

Abstract The objective of this experiment was to investigate the effects of Bacillus subtilis on growth performance, diarrhea and fecal β-hemolytic coliforms of weaned pigs experimentally infected with a strain of E. coli (F18, express genes of LT, STb, and SLT 2 toxins). Weaned pigs (n = 48, 6.17 ± 0.36 kg BW) were individually housed in disease containment rooms and randomly allotted to one of four dietary treatments: negative control (NC, control diet without E. coli challenge), positive control (PC, control diet with E. coli challenge), and supplementation of 50 mg/kg of carbadox or 500 mg/kg of Bacillus subtilis probiotics. The experiment lasted 28 d with 7 d before and 21 d after the first E. coli inoculation. The F18 E. coli were given to pigs at 1010 CFU/3 mL dose for three consecutive d. Diarrhea score was daily recorded for each pig to calculate frequency of diarrhea. Fecal samples were collected on d 0, 3, 7, 14, and 21 PI to analyze β-hemolytic coliforms. Data were analyzed using the Mixed Procedure of SAS. Pigs supplemented with carbadox had greater (P < 0.05) body weight on d 7, 14, and 21 PI than pigs in the PC and probiotics group. Supplementation of probiotics enhanced pig body weight on d 21 PI, compared with the PC. E. coli challenge reduced (P < 0.05) ADG and feed efficiency from d 0 to 21 PI, while supplementation of antibiotics or probiotics enhanced ADG and feed efficiency from d 0 to 21 PI. Pigs in carbadox and probiotics groups had reduced (P < 0.05) frequency of diarrhea throughout the experiment and fecal β-hemolytic coliforms on d 7 PI than pigs in the PC. In conclusion, supplementation of Bacillus subtilis could enhance disease resistance and promote growth performance of weaned pigs under disease challenge condition.

scholarly journals PSVIII-2 Dietary supplementation of Bacillus subtilis altered metabolites in the intestine of weaned pigs

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 208-208
Author(s):  
Yijie He ◽  
Kwangwook Kim ◽  
Cynthia Jinno ◽  
Xunde Li ◽  
Yanhong Liu
Keyword(s):  
Bacillus Subtilis ◽  
Statistical Significance ◽  
Control Diet ◽  
Dietary Supplementation ◽  
Enterotoxigenic Escherichia Coli ◽  
P Value ◽  
Weaned Pigs ◽  
Ileal Mucosa ◽  
Galactose Metabolism ◽  
E Coli

Abstract This experiment aimed to investigate the effects of dietary supplementation of Bacillus subtilis probiotics on metabolites in the intestines of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli (E. coli). Forty-eight weaned pigs (6.17 ± 0.36 kg BW) were individually housed in disease containment rooms and randomly allotted to one of four dietary treatments: negative control (NC, control diet without E. coli challenge), positive control (PC, control diet with E. coli challenge), and supplementation of 50 mg/kg of carbadox or 500 mg/kg of Bacillus subtilis probiotics. The experiment lasted 28 days with 7 days before and 21 days after the first E. coli inoculation. The F18 E. coli were given to pigs at 1010 CFU/3 mL dose for three consecutive days. At the end of the experiment, all pigs were euthanized to collect ileal mucosa and colon digesta for the analysis of metabolomic profiles by gas chromatography time of flight-mass spectrometer (GCTOF-MS). All data were analyzed by an online MetaboAnalyst tool (https://www.metaboanalyst.ca/). Statistical significance was declared at P &lt; 0.05 and the false discovery rate–adjusted P value (q value) &lt; 0.20. A total of 282 (141 identified and 121 unidentified) and 196 (127 identified and 69 unidentified) metabolites were detected in ileal mucosa and colon digesta, respectively. Forty-nine identified metabolites in ileal mucosa significantly differed among experimental groups (P &lt; 0.05; q &lt; 0.20). The most impacted metabolic pathways were galactose metabolism, aspartate and glutamate metabolism, fructose and mannose degradation, pentose phosphate pathway, and urea cycle. However, in colon digesta, only 7 identified metabolites differed among experimental groups (P &lt; 0.05; q &lt; 0.20) and the majority of them were involved in purine metabolism. Results of metabolomics indicated that supplementation of Bacillus subtilis or antibiotics altered metabolites in the intestines of weaned pigs. In particular, more treatment impacts were observed in the metabolite profiles in ileal mucosa compared with colon digesta.

scholarly journals Bacillus subtilis: a potential growth promoter in weaned pigs in comparison to carbadox

2020 ◽  
Vol 98 (9) ◽  
Author(s):  
Yijie He ◽  
Kwangwook Kim ◽  
Lauren Kovanda ◽  
Cynthia Jinno ◽  
Minho Song ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Blood Cell ◽  
Growth Performance ◽  
Feed Efficiency ◽  
Control Diet ◽  
Weaned Pigs ◽  
Ileal Mucosa ◽  
E Coli

Abstract The study was conducted to investigate the efficacy of a probiotic Bacillus subtilis strain on growth performance, diarrhea, systemic immunity, and intestinal health of weaned pigs experimentally infected with an enterotoxigenic Escherichia coli and to compare the efficacy of B. subtilis with that of carbadox. Weaned pigs (n = 48, 6.17 ± 0.36 kg body weight [BW]) were individually housed in disease containment rooms and randomly allotted to one of four dietary treatments: negative control (NC, control diet without E. coli challenge), positive control (PC, control diet with E. coli challenge), and supplementation of 50 mg/kg of carbadox (antibiotic growth promotor [AGP]) or 2.56 × 109 CFU/kg of B. subtilis probiotics (PRO). The experiment lasted for 28 d with 7 d before and 21 d after the first E. coli inoculation. Fecal and blood samples were collected on days 0, 3, 7, 14, and 21 post inoculation (PI) to analyze β-hemolytic coliforms and complete blood cell count, respectively. Diarrhea score was recorded daily for each pig to calculate the frequency of diarrhea. All pigs were euthanized at day 21 PI to collect jejunal and ileal mucosa for gene expression analysis. Pigs in AGP had greater (P &lt; 0.05) BW on days 7, 14, and 21 PI than pigs in PC and PRO groups. Supplementation of PRO enhanced pigs’ BW on day 21 PI compared with the PC. Escherichia coli F18 challenge reduced (P &lt; 0.05) average daily gain (ADG) and feed efficiency from day 0 to 21 PI, while supplementation of carbadox or PRO enhanced ADG and feed efficiency in E. coli F18-challenged pigs from day 0 to 21 PI. Pigs in AGP and PRO groups had reduced (P &lt; 0.05) frequency of diarrhea throughout the experiment and fecal β-hemolytic coliforms on day 7 PI than pigs in the PC. Pigs in PRO had greater (P &lt; 0.05) gene expression of CLDN1 in jejunal mucosa than pigs in the PC. Supplementation of carbadox or PRO reduced (P &lt; 0.05) the gene expression of IL6 and PTGS2 in ileal mucosa of E. coli-infected pigs compared with pigs in the PC. Pigs in the PRO group had lower (P &lt; 0.05) white blood cell number and neutrophil count, and serum haptoglobin concentration on day 7 PI, and less (P &lt; 0.05) monocyte count on day 14 PI, compared with PC. In conclusion, supplementation of probiotic B. subtilis could enhance disease resistance and promote the growth performance of weaned pigs under disease challenge conditions. The potential mechanisms include but not limited to enhanced gut barrier integrity and local and systemic immune responses of weaned pigs.

scholarly journals 81 Effects of NeutraPath™ on growth performance, diarrhea and fecal-hemolytic coliforms of weaned pigs challenged with an enterotoxigenic Escherichia coli

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 4-5
Author(s):  
LeAnn Johnston ◽  
Peng Ji ◽  
Hongyu Xue
Keyword(s):  
Growth Performance ◽  
Repeated Measures ◽  
Area Under The Curve ◽  
Enterotoxigenic Escherichia Coli ◽  
Post Inoculation ◽  
Weaned Pigs ◽  
E Coli ◽  
Severe Diarrhea ◽  
Diarrhea Incidence ◽  
And Gender

Abstract This study was aimed to investigate NeutraPath’s effects on performance, diarrhea, and fecal β-hemolytic coliforms of weaned pigs infected with enterotoxigenic E. coli (ETEC). NeutraPath, a blend of essential oils, fatty acids and toxin-adsorbing minerals has bacteriostatic/bactericidal effects. Thirty-six weanling pigs (21 d old; average BW 6.88 kg; 18 barrows, 18 gilts) blocked by weight and gender, were assigned to one of three dietary treatments in an RCB (1 pig/pen; 12 replicates/treatment). Treatments were control or dietary supplementation with 0.25% or 0.50% NeutraPath. This study lasted 28 d with 7 d before and 21 d after the first E. coli inoculation (d 0). All pigs were orally inoculated with 1010 cfu F18+ ETEC/3-mL dose for 3 consecutive d. Growth performance data was obtained on d 0, d 7, d 14 and d 21 post-inoculation (PI). On d 2 PI, fecal samples were obtained and plated on blood and MacConkey agars to determine total coliforms and β-hemolytic coliforms. Treatment effects were analyzed using one-way ANOVA or repeated measures ANOVA followed by post hoc Tukey’s test. The 0.5% NeutraPath treatment resulted in a 12.7% and a 14.0% increase (P &lt; 0.05) in BW (relative to initial BW at d 0 PI) at d 7 PI and d 21 PI, respectively. Pigs fed either NeutraPath dose had greater overall growth during the 28-d period (P&lt; 0.05) producing improvements in feed efficiency during d 14-21 PI (P&lt; 0.05). Feeding 0.25% NeutraPath improved (P &lt; 0.05) diarrhea kinetics d 0-11 PI, Diarrhea score (DS) area under the curve for d 0-11 and d 0-21 PI, moderate (DS≥3) and severe (DS≥4) diarrhea incidence and tended (P=0.08) to lower the percentage of fecal β-hemolytic coliforms compared to control. In conclusion, NeutraPath supplementation can enhance growth performance and decrease incidence of severe diarrhea in weaned pigs challenged with F18+ ETEC.

187 Effects of Oligosaccharide-based Polymer on Growth Performance, Diarrhea, and Fecal β-hemolytic Coliforms in Weanling Pigs Experimentally Infected with a Pathogenic E. coli

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 90-90
Author(s):  
Kwangwook Kim ◽  
Yijie He ◽  
Cynthia Jinno ◽  
Seijoo Yang ◽  
Xunde Li ◽  
...  
Keyword(s):  
Growth Performance ◽  
Feed Efficiency ◽  
Basal Diet ◽  
Watery Diarrhea ◽  
Experimental Unit ◽  
Post Inoculation ◽  
Weaned Pigs ◽  
E Coli ◽  
Diet Control ◽  
Dietary Treatments

Abstract The objective of this experiment was to investigate dietary supplementation of oligosaccharide-based polymer on growth performance, diarrhea, and fecal β-hemolytic coliforms of weaned pigs experimentally infected with a pathogenic F18 Escherichia coli (E. coli). Forty-eight pigs (7.23 ± 1.11 kg BW) were individually housed in disease containment rooms and randomly allotted to one of four treatments with 12 replicate pigs per treatment. The four dietary treatments were a nursery basal diet (control), and 3 additional diets supplemented with 50 mg/kg Mecadox (AGP), 10 or 20 mg/kg of oligosaccharide-based polymer. The experiment lasted 18 d [7 d before and 11 d after the first inoculation (d 0)]. The doses of F18 E. coli inoculum were 1010 cfu/3 mL oral dose daily for 3 days. Growth performance was measured on d -7 to 0 before inoculation, and d 0 to 5 and 5 to 11 post-inoculation (PI). Diarrhea score (DS; 1, normal, to 5, watery diarrhea) was daily recorded for each pig. Fecal samples were collected on d 2, 5, 8, and 11 PI to test the percentage of β-hemolytic coliforms in total coliforms. All data were analyzed by ANOVA using the PROC MIXED of SAS with pig as the experimental unit. Inclusion of oligosaccharide-based polymer linearly increased (P &lt; 0.05) ADFI on d 0 to 5 PI, and feed efficiency on d 0 to 5 PI and d 5 to 11 PI (P = 0.07), compared with the control. Supplementation of AGP or oligosaccharide-based polymer reduced (P &lt; 0.01) frequency of diarrhea of pigs from d 0 to 11 PI. No differences were observed in overall growth performance and percentage of fecal β-hemolytic coliforms on d 8 PI among pigs in AGP and oligosaccharide-based polymer treatments. In conclusion, supplementation of oligosaccharide-based polymer enhanced feed efficiency and reduced diarrhea of weaned pigs infected with a pathogenic E. coli.

Sign in / Sign up

Export Citation Format

Share Document