scholarly journals 339 Effect of DHA and EPA supplementation during the first third of gestation on growth, metabolism and gene expression in hypothalamus in finished lambs

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 139-140
Author(s):  
Mario Francisco Oviedo ◽  
Jose Alejandro Roque ◽  
Hector Aaron Lee ◽  
Alejandro E Relling
Keyword(s):  
Fatty Acids ◽  
Mixed Model ◽  
Leptin Receptor ◽  
Repeated Measurements ◽  
Late Gestation ◽  
Plasma Metabolites ◽  
Productive Performance ◽  
Finishing Diets ◽  
Mrna Concentration ◽  
Daily Body Weight

Abstract Supplementation with fatty acids (FA) during late gestation regulates offspring development; however, its effect in the first third of gestation is unknown. The objective of this study was to evaluate the effects of with eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) supplementation during the first third of gestation on productive performance and hypothalamic neuropeptides on the offspring. Seventy-nine post-weaning lambs, born of sheep supplemented in the first third of gestation with 1.5% Ca salts rich in monounsaturated FA (MUFA) or PUFA [DHA and EPA; Dam supplementation (DS)], were distributed in a 2x2 factorial arrangement of treatments to finishing diets containing MUFA or PUFA [Lamb supplementation (LS)]. The experiment last for 56 d. Measurements dry matter intake (daily), body weight and plasma metabolites (every 14 d) were taken. Twenty-four lambs were slaughtered and hypothalamus were obtained for mRNA concentration. The data were analyzed with a mixed model in SAS (9.4) using repeated measurements. There was a DS x LS interaction for BW (P < 0.10) where LS with PUFA born from DS with MUFA were heavier than the other 3 treatments. Lambs born from DS with MUFA have a greater DMI (P < 0.01). There was a time x DS effect (P < 0.05) for plasma glucose and non-esterified fatty acids (NEFA) concentration, due to a greater concentration for glucose and lower for NEFA on the lambs born from DS-MUFA at weaning but not on d 56. Lambs born from MUFA supplemented dams had a greater (P ≤ 0.05) hypothalamus mRNA concentration for CART, GH-receptor, KISS-1, leptin receptor, POMC, and NPY receptor Y1. There were no LS effects (P > 0.05) for these neuropeptides. These results indicate that supplementation with fatty acids during early pregnancy change productive performance, metabolic, and neuropeptides of lambs independently of the final diet.

scholarly journals Effect of supplementation with different fatty acid profile to the dam in early gestation and to the offspring on the finishing diet on offspring growth and hypothalamus mRNA expression in sheep

2021 ◽  
Author(s):  
Mario Francisco Oviedo-Ojeda ◽  
José Alejandro Roque-Jiménez ◽  
Megan Whalin ◽  
Héctor Aarón Lee-Rangel ◽  
Alejandro Enrique Relling
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Mrna Expression ◽  
Mixed Model ◽  
Hormone Receptors ◽  
Leptin Receptor ◽  
Repeated Measurements ◽  
Late Gestation ◽  
Metastasis Suppressor ◽  
Productive Performance

Abstract Supplementation with omega-3 and omega-9 fatty acids during late gestation regulates offspring development; however, their effect in the first third of gestation is unknown in sheep. The objective of this experiment was to evaluate the effects of the maternal supplementation with an enriched source of monounsaturated fatty acid (MUFA) or an enriched source of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) during the first third of gestation on productive performance on ewes and offspring, and hypothalamic neuropeptides on offspring. Seventy-nine post-weaning lambs, born of sheep supplemented in the first third of gestation with 1.61% Ca salts rich with MUFA or EPA+DHA (Dam supplementation; DS), were distributed in a 2×2 factorial arrangement of treatments to finishing diets containing 1.48% of Ca salts of MUFA or EPA+DHA (Lamb supplementation; LS). The finishing period of the offspring lasted for 56 d. During the finishing period dry matter intake (DMI, daily) and body weight (BW) were recorded. Plasma was collected for metabolites analysis. Twenty-four lambs were slaughtered, and hypothalamus was collected for mRNA expression of hormone receptors, neuropeptides, and lipid transport genes. The data were analyzed with a mixed model in SAS (9.4) using repeated measurements, when needed. There was a DS×LS interaction for BW (P = 0.10) where LS with EPA+DHA born from DS with MUFA were heavier than the other 3 treatments. Lambs born from DS with MUFA have a greater DMI (P &lt; 0.01) than the offspring born from DS with EPA+DHA. Lambs born from MUFA supplemented dams had a greater (P ≤ 0.05) hypothalamus mRNA expression for cocaine and amphetamine regulated transcript, growth hormone receptor, metastasis suppressor 1, leptin receptor, pro-opiomelanocortin, and Neuropeptide Y. These results indicate that growth depends not on the type of fatty acids during the finishing phase but the interaction of different sources of fatty acids ad different stages. Also, supplementation with fatty acids during early pregnancy changes productive performance and neuropeptides’ mRNA expression of lambs independently of the finishing diet.

scholarly journals 338 Effect of eicosapentaenoic acid and docosahexaenoic acid supplementation on placenta and fetal liver mRNA, and fetal liver and brain fatty acids profile on early gestation in sheep

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 139-139
Author(s):  
Jose Alejandro Roque ◽  
Mario Francisco Oviedo ◽  
Hector Aaron Lee ◽  
Alejandro E Relling
Keyword(s):  
Fatty Acids ◽  
Docosahexaenoic Acid ◽  
Eicosapentaenoic Acid ◽  
Mrna Expression ◽  
Fetal Liver ◽  
Cell Metabolism ◽  
Late Gestation ◽  
Early Gestation ◽  
Fatty Acids Profile ◽  
Mrna Concentration

Abstract Polyunsaturated fatty acids supplementation in late gestation change offspring metabolism; however, their effect is not well known on early gestation in ewes. The objectives of this study were to determine the effect of dietary supplementation with eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in pregnant ewes on the concentration of EPA and DHA on fetal liver (FL) and fetal central nervous system (FCNS), and to evaluate the effect of the supplementation with EPA+ DHA on mRNA expression of genes associated with transport and metabolism of fatty acids (FA) in FL and placenta (caruncles and cotyledons). Twelve ewes (4 pens, three per pen) were blocked by pregnancy day. The ewes were assigned during the first 45 d of gestation to diet with an addition of 1.5% (dry matter bases) monounsaturated FA (MUFA) or EPA+DHA. A C-section was conducted at d 45 of gestation to collect FL, FCNS, caruncle and cotyledon. Data were analyzed using a mixed procedure (SAS). For the placenta mRNA concentration, a 2x2 factorial was used considering caruncle and cotyledon as the second main factor. Isomers of C18:1 (t6,8 and t12) increase (P < 0.05) in FL and FCNS with MUFA supplementation, fatty acids C20:3 (n-6), C20:3 (n-3), C22:1, C22:5 and C22:6 increase (P < 0.05) in FL and FCNS with EPA+DHA supplementation. In FL there was a tendency to increase for mRNA expression of FATP-1 (P = 0.10) with EPA+DHA supplementation, while mRNA concentration for LPL was greater (P = 0.02) for MUFA supplementation. In placenta DNMT3b and FFAR-4 showed a significant FA x tissue interaction (P < 0.05). These results suggest that FA supplementation during early gestation alters the FA profile in FL and FCNS and changed mRNA concentration of genes involved in the transport of FA and cell metabolism.

scholarly journals PSVI-21 Effect of preconditioning pre-shipment and injectable antioxidant trace elements and vitamins on growth, antioxidant status, and plasma metabolites and insulin concentration in weaned beef cattle

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 219-219
Author(s):  
Raul M Lizarraga ◽  
Esteban Galarza ◽  
Guillermo Mattioli ◽  
Anthony J Parker ◽  
Alejandro E Relling
Keyword(s):  
Body Weight ◽  
Antioxidant Status ◽  
Mixed Model ◽  
Total Antioxidant Status ◽  
Repeated Measurements ◽  
Vitamin Supplementation ◽  
Plasma Metabolites ◽  
Nefa Concentration ◽  
Triple Interaction ◽  
Total Antioxidant

Abstract The aim of this study was to evaluate the effect of weaning preconditioning and parenteral supplementation of minerals and vitamin on growth and plasma total antioxidant status (TAS), glucose, NEFA, and insulin concentrations, in calves at feedlot arrival. Sixty male Angus-cross calves were randomly assigned to 4 treatments (n=15/treatment). Treatments were arranged in a 2x2 factorial design; the main factors were mineral and vitamins supplementation (supplementation (S) vs no supplementation (NS)), and preconditioning (preconditioning (P) vs. not (NP)). Supplementation was applied subcutaneously on d -45, -26 and 0 and provided Cu, Mn, Se, Zn, and vitamins A and E (Adaptador Min-Vit ®, Biogénesis Bagó). Preconditioning consisted in a 26-day period between weaning and transportation at feedlot (d0) with the animals remaining in the same location. Blood samples were collected on d -26, 0 and 15. Animals were weighted on d-45, -26, 0, 8, 15 and 29. On d0 calves were load into a truck and drove for 5-hours to the feedlot. Data were analyzed using a mixed model as a 2x2 factorial, using repeated measurements. Body weight was greater on d 0, 8 15 and 29 group on groups NP, followed by S-P, and the lowest for NS-P (Triple interaction P &lt; 0.01). Treatment did not affect (P ˃ 0.1) plasma insulin and glucose concentrations. There was a triple interaction (P &lt; 0.08) for plasma TAS and NEFA concentration. Plasma TAS concentration was grater due to a decrease in its concentration for the S-P and NS-NP from d0 to d8, but not in the other treatments. Plasma NEFA concentration had the greater increase in the NP groups followed by S-NP on d0. In conclusion, 26-days precondition weaning decrease body weight compare with a traditional weaning. Mineral and vitamin supplementation in precondition weaning partially reduced that effect

scholarly journals Supplementation with eicosapentaenoic and docosahexaenoic acids in late gestation in ewes changes adipose tissue gene expression in the ewe and growth and plasma concentration of ghrelin in the offspring1

2019 ◽  
Vol 97 (6) ◽  
pp. 2631-2643
Author(s):  
Kirsten R Nickles ◽  
Lauren Hamer ◽  
Danielle N Coleman ◽  
Alejandro E Relling
Keyword(s):  
Adipose Tissue ◽  
Milk Fat ◽  
Dry Matter ◽  
Positive Impact ◽  
Block Design ◽  
Repeated Measurements ◽  
Late Gestation ◽  
Dry Matter Intake ◽  
Plasma Metabolites ◽  
Epa And Dha

Abstract Omega-3 long chain fatty acids have a positive impact on production. When consumed during late gestation, it might have fetal programming effects on the fetus, which will have lifelong impacts on development and production. The objectives of the present study were to evaluate the effect of increasing doses of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in the diet of ewes in the last third of gestation on their body weight (BW), subcutaneous adipose tissue relative mRNA abundance of genes associated with adipose tissue metabolism, and growth performance and plasma metabolites and hormones of their offspring during the finishing phase. Ewes (n = 72) were blocked by BW and allotted to pens (8 per treatment) with 3 ewes per pen. Ewes were supplemented with an EPA and DHA source (Strata G113) at concentrations of 0, 1, or 2% of dry matter intake during the last 50 d of gestation. At lambing, all ewes were penned together and offered the same diet. After weaning at 60 d of age, lambs were blocked by BW and sex and fed for 56 d. All lambs were fed the same pellet diet (61.09% ground corn, 24.08% soy hulls, 11.09% soybean meal, 1.48% Ca salt of palm oil, and 2.26% mixed mineral vitamin), and were weighed every 14 d until the end of the trial. Blood samples were collected on the weight sampling days. Dry matter intake and refusals were weighed daily. Data were analyzed as a randomized complete block design with repeated measurements (SAS 9.4). Polynomial contrast (linear-L and quadratic-Q) was used for mean separation. There were no differences in ewe body condition score, milk production, milk fat, or milk protein, but there was a trend for increased (L, P = 0.06) lactose concentration, and also differences in DGAT1 (L, P = 0.04), Δ5-desaturase (Q, P = 0.06) and Δ6-desaturase (Q, P = 0.07), PPARα (Q, P = 0.03), ELOVL2 and 5 (Q, P &lt; 0.07), FABP4 (Q, P = 0.04), FATP1 (Q, P = 0.06), leptin (Q, P = 0.02), and resistin (L, P = 0.05). Feeding pregnant ewes an increased amount of EPA and DHA in late gestation increased final BW (L, P = 0.01), ADG (L, P = 0.04; Q, P = 0.01), DMI (Q, P ≤ 0.01), plasma glucose concentration (L, P = 0.04), and trended to decrease ghrelin concentrations (L, P = 0.07) in offspring during the finishing period. Dam supplementation did not affect G:F, nor plasma NEFA concentration (P ≥ 0.53) of lambs. Therefore, increasing supplementation of EPA and DHA in pregnant ewes has an impact on offspring performance, increasing DMI, ADG, and BW.

scholarly journals PSIV-8 Effects of Late Gestation Calcium Salts of Fatty Acids Supplementation to Beef Cows on Offspring Finishing Phase Performance and Carcass Characteristics

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 284-284
Author(s):  
Taoqi Shao ◽  
Joshua C McCann ◽  
Daniel W Shike
Keyword(s):  
Fatty Acids ◽  
Growth Performance ◽  
Carcass Characteristics ◽  
Beef Cows ◽  
Late Gestation ◽  
Repeated Measure ◽  
Feed Conversion ◽  
Marbling Score ◽  
Finishing Diets ◽  
System Data

Abstract The objective was to investigate the effects of late-gestation supplementation of Ca salts of fatty acids to beef cows on the finishing phase growth performance and carcass characteristics of the steer progeny. Ninety-six multiparous, fall-calving, Angus × Simmental cows (BW = 601 ± 76 kg) were stratified by BW, BCS, and age and randomly allotted into 8 endophyte-infected tall fescue pastures with 12 cows/pasture. Cows were supplemented with soybean hulls mixed with either 155 g/cow/d of EnerGII (SFA/MUFA; enriched with palmitic and oleic acids) or 80 g/cow/d of Prequel + 80 g/cow/d of Strata (PUFA; enriched with linoleic acid, EPA, and DHA) for the last 77 ± 6 d of gestation. Steer progeny (n = 70) were weaned at 186 ± 6 d of age and transported to feedlot. Steers were fed receiving diets for 3 weeks and transitioned to finishing diets and adapted to GrowSafe feed bunks for individual daily feed intake measurement. Steers were weighed every 28 d during the finishing phase. Steers were slaughtered at commercial abattoir at 432 ± 6 d of age, and carcass data were obtained from USDA camera grading system. Data were analyzed using the MIXED procedure of SAS, and repeated measure was used for BW. There were no differences (P ≥ 0.13) for BW, ADG, DMI, or feed conversion during the finishing phase. There was no differences (P ≥ 0.22) observed in hot carcass weight, dressing percentage, 12th rib fat thickness, yield grade, ribeye area, marbling score, or KPH fat. In conclusion, maternal supplementation of Ca salts of fatty acids during late gestation did not affect steer progeny growth performance during the finishing phase or carcass characteristics.

scholarly journals PSVII-18 Fatty acid composition of the longissimus muscle from Angus and Wagyu sired cattle at the 6th and 12th rib location

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 211-212
Author(s):  
Jerad Jaborek ◽  
Francis L Fluharty ◽  
Alejandro E Relling
Keyword(s):  
Fatty Acid ◽  
Mixed Model ◽  
Random Effect ◽  
Repeated Measurements ◽  
Longissimus Muscle ◽  
Trans Isomers ◽  
Fa Composition ◽  
Effect Of Treatment ◽  
Similar Body ◽  
Location Interaction

Abstract The fatty acid (FA) composition of the longissimus muscle (LM) of Angus and Wagyu sired cattle raised to a similar body weight (612 kg) were compared at the 6th and 12th rib locations. Angus sired steers represented T1, cattle from a Wagyu sire selected for growth represented T2, and cattle from a Wagyu sire selected for marbling represented T3. Data were analyzed mixed model with repeated measurements on animal (LM location); the model include the fixed effect of treatment, LM location, and their interaction, and random effect of sex. The percentage of 16:0, 18:1cis9, 18:3, and monounsaturated FA (MUFA) exhibited a treatment*LM location interaction (P ≤ 0.7), where T2 cattle had a greater percentage of 16:0 and a lesser percentage of 18:1cis9, 18:3, and MUFA at the 12th rib vs. 6th rib location compared with T1 and T3 cattle. The percentage of total FA lipid, polyunsaturated FA(PUFA), and PUFA:SFA ratio in the LM were greater (P ≤ 0.02) for T3 cattle compared with T1 and T2 cattle. The percentage of 18:0 was greater (P ≤ 0.01) for T1 cattle compared with T2 and T3 cattle, while T1 cattle had a greater (P ≤ 0.01) percentage of saturated FA (SFA) compared to T3 cattle. The percentage of 18:1cis9, other 18:1cis isomers, 18:2, MUFA, and MUFA:SFA ratio were greater (P ≤ 0.02) for T3 cattle compared with T1 cattle, with T2 cattle being intermediate. The percentage of total FA lipid, 18:0, 18:1 trans isomers, and SFA were greater (P ≤ 0.01) at the 6th rib LM location, while 14:1, 18:cis9, other 18:1 cis isomers, MUFA, MUFA:SFA, and PUFA:SFA ratio were greater (P ≤ 0.02) at the 12th rib LM location

scholarly journals Mixed Models as a Tool for Comparing Groups of Time Series in Plant Sciences

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 362
Author(s):  
Ioannis Spyroglou ◽  
Jan Skalák ◽  
Veronika Balakhonova ◽  
Zuzana Benedikty ◽  
Alexandros G. Rigas ◽  
...  
Keyword(s):  
Time Series ◽  
Random Effects ◽  
Mixed Models ◽  
Mixed Model ◽  
Repeated Measurements ◽  
Time Series Models ◽  
Additional Time ◽  
Biotic Stresses ◽  
Viable Solution ◽  
Long Time

Plants adapt to continual changes in environmental conditions throughout their life spans. High-throughput phenotyping methods have been developed to noninvasively monitor the physiological responses to abiotic/biotic stresses on a scale spanning a long time, covering most of the vegetative and reproductive stages. However, some of the physiological events comprise almost immediate and very fast responses towards the changing environment which might be overlooked in long-term observations. Additionally, there are certain technical difficulties and restrictions in analyzing phenotyping data, especially when dealing with repeated measurements. In this study, a method for comparing means at different time points using generalized linear mixed models combined with classical time series models is presented. As an example, we use multiple chlorophyll time series measurements from different genotypes. The use of additional time series models as random effects is essential as the residuals of the initial mixed model may contain autocorrelations that bias the result. The nature of mixed models offers a viable solution as these can incorporate time series models for residuals as random effects. The results from analyzing chlorophyll content time series show that the autocorrelation is successfully eliminated from the residuals and incorporated into the final model. This allows the use of statistical inference.

528 Late-Breaking: Late Gestation Metabolizable Energy Intake Increases Colostrum Yield and Alters Colostrum Composition in Beef Cattle

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 150-150
Author(s):  
Koryn S Hare ◽  
Emily Croft ◽  
Katharine M Wood ◽  
Michael A Steele
Keyword(s):  
Fixed Effects ◽  
Block Design ◽  
Compositional Analysis ◽  
Repeated Measurements ◽  
Metabolizable Energy ◽  
Late Gestation ◽  
Beef Cow ◽  
Randomized Block Design ◽  
Milking Machine ◽  
Beta Hydroxybutyrate

Abstract The objective was to determine how late gestation metabolizable energy (ME) intake impacts beef cow colostrogenesis. Angus-Simmental primiparous (n = 47) and multiparous (n = 109) cattle were blocked by expected calving date and randomly assigned to receive diets that supplied 80% (LME; n = 53), 100% (CME; n = 52), or 120% (HME; n = 51) of the ME requirement. Treatments were balanced by initial BW and fed for 53 d prior to calving. Cow body weight was recorded d -53, -39, -25, -10, and -3 relative to calving and retrospectively conceptus-corrected. Calves were weighed and measured prior to suckling. Colostrum was hand-stripped from all cows for compositional analysis and a subset (n = 16/treatment) was fully milked using a milking machine. Data were analyzed (PROC GLIMMIX) as a randomized block design, including the fixed effects of treatment, parity, and treatment×parity and the random effects of block and cow(block). Day and its interactions were included for repeated measurements. Treatment did not affect (P ≥ 0.73) cow BW or conceptus-corrected BW. Calf chest circumference was lesser (treatment×parity: P = 0.023) for primiparous vs. multiparous calves if their dams consumed LME or CME, but not if their dams consumed HME. Feeding HME rather than LME increased (P = 0.006) colostrum yield. The LME diet increased (P = 0.004) colostrum IgG concentration over HME; however, HME colostrum yielded more (P = 0.005) total IgG. Crude protein and urea-N were elevated (P &lt; 0.001) in LME colostrum, whereas HME colostrum contained more (P = 0.026) lactose. Low ME colostrum fat was greater (P = 0.013) than CME and beta-hydroxybutyrate was increased (P &lt; 0.001) in LME vs. CME and HME colostrum. These data provide insight regarding nutrient partitioning during late gestation and demonstrate that beef cow colostrogenesis is responsive to prepartum ME intake.

Abstract WP138: Effects of Intensive Medical Management (IMM) and Encephaloduroarteriosynangiosis (EDAS) On VEGFA165a/b Ratios in Patients With Intracranial Atherosclerosis (ICASi

Stroke ◽  
2016 ◽  
Vol 47 (suppl_1) ◽  
Author(s):  
Nestor R Gonzalez ◽  
Raymond Liou ◽  
Yinn Ooi ◽  
Jason D Hinman ◽  
Neal Rao ◽  
...  
Keyword(s):  
Prospective Observational Study ◽  
Mixed Model ◽  
Sandwich Elisa ◽  
Repeated Measurements ◽  
Likelihood Method ◽  
Outcome Variable ◽  
Serum Samples ◽  
Persistent Symptoms ◽  
Early Elevation

Objective: VEGFA isoforms 165a and 165b are pro and antiangiogenic, respectively. We aimed to evaluate the effects of IMM and EDAS in the VEGFA165a/b ratio in patients with ICAS. Methods: This is a prospective observational study of VEGFA165a and b in patients with stenosis greater than 70% due to ICAS. All patients received IMM. Patients with persistent symptoms underwent EDAS while maintaining IMM. Serum samples were collected at baseline, 1 week, 1, 3, and 6 months. VEGFA isoforms were quantified using multiplex sandwich ELISA. All samples were run in duplicate and accepted as valid if the intersample variability was less than 20%. A mixed model was built for the outcome variable VEGFA165a/b ratio using the predictor variables timepoint, treatment, and the interaction of time and treatment. The restricted maximum likelihood method was used to fit the model with random effects to account for the repeated measurements and intersubject variability. Results: A total of 72 patients were enrolled, of which 58 had IMM alone and 14 had EDAS. Mean age was 61.8 ± 12.3, 53% were females. The regression model demonstrated that there were no significant differences in the VEGFA165a/b ratio at baseline and 1 month after enrollment. Significant differences in VEGFA165a/b ratio were found at one week with higher levels in the surgical group (EDAS: 0.46 ± 0.22, IMM: 0.24 ± 0.07, p=0.03) and at 3 and 6 months with higher levels in the IMM group (3m: EDAS: 0.29 ± 0.14, IMM: 0.45 ± 0.20, p=0.03, 6m: EDAS:0.19 ± 0.11, IMM 0.37 ± 0.19 p=0.01). Conclusion: While the surgical event may well explain the early elevation of the VEGFA165a/b ratio one week after surgery, the elevation of a proangiogenic profile by the 3rd and 6th month in the IMM group is relevant. None of the patients in the IMM or EDAS groups had strokes at the last 6 months follow-up, and the peak (early for EDAS and at 3 and 6 months for IMM) of the VEGFA165a/b ratio may indicate a protective effect, averting stroke.

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