scholarly journals PSXIV-4 Fetal programming effect of rumen-protected methionine on Angus-Simental heifer offspring’s performance and adipogenesis, oxidative stress and DNA methylation gene expression regulation

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 307-308
Author(s):  
Sonia J Moisá ◽  
Gaston F Alfaro ◽  
Paul H Walz ◽  
Soren P Rodning
Keyword(s):  
Milk Production ◽  
Messenger Rna ◽  
Gene Expression Regulation ◽  
Treatment Time ◽  
Control Diet ◽  
Average Daily Gain ◽  
Late Gestation ◽  
Positive Effects ◽  
Ad Libitum ◽  
Dietary Treatments

Abstract An extensive body of literature reported positive effects of Rumen-Protected Methionine (RPM) on dairy cattle; however, its effects are unknown on beef cattle. The objective of this study was to assess the effect of RPM supplementation on Angus-Simmental heifer’s offspring during late gestation, lactation, and after weaning (~65d). Ninety days prior to calving, 22 Angus-Simmental heifers were stratified by body weight (454 ± 85 kg; BW) and randomly assigned to dietary treatments: 1) Control diet (CTRL), and 2) Control diet + RPM (RPM). Dams had access to Bermuda hay ad libitum, and a soy hull:corn gluten based supplement. After weaning, calves were allocated by dietary treatments based on their dam’s treatment. Calves received Bermuda hay ad libitum and early-wean feed supplement after weaning. Offspring’s BW and Longissimus dorsi (LM) muscle samples were obtained on days 0, 25, 50, 100 after weaning. Messenger RNA was extracted from LM for RT-qPCR analysis. There was a tendency for RPM calves to be heavier at birth compared to control calves (P = 0.07). Although, no differences in BW were detected at weaning and after weaning (P = 0.20). Calves average daily gain between birth and weaning was greater for RPM calves as compared to CON (P = 0.02) suggesting a greater milk production, with no differences after weaning (P = 0.53). RT-qPCR results showed a treatment × time interaction for PPARG, LPL, CEBPG, DNMT1, SOD2 and NOS3 (P < 0.05). Furthermore, PPARG had a treatment and a time effect, which was upregulated in RPM calves (P < 0.01) from 50 to 100 days after weaning. In conclusion, dam’s RPM supplementation during late gestation increased offspring’s BW when inside the womb and probably their milk production. Offspring’s RPM supplementation after weaning potentially stimulates an early differentiation of adipocytes in LM samples, detected by PPARG upregulation.

Effects of housing beef cow-calf pairs on drylot or pasture in the Midwest on production parameters and calf behavior through feedlot receiving

2021 ◽  
Author(s):  
Megan E Myerscough ◽  
Lucas T Neira ◽  
Keifer H Sexton ◽  
Lucas S Hofer ◽  
Keela M Trennepohl ◽  
...  
Keyword(s):  
Milk Production ◽  
Treatment Time ◽  
Body Condition Score ◽  
Average Daily Gain ◽  
Beef Cow ◽  
Condition Score ◽  
Ad Libitum ◽  
Cow Calf ◽  
Cow Performance ◽  
And Behavior

Abstract The objectives were to analyze the effects of housing cow-calf pairs in drylots (DL) or pasture (PAST) on cow performance and reproduction as well as calf performance and behavior through feedlot receiving. Simmental × Angus (2 yr; 108/yr; 81 ± 15.3 d postpartum) spring-calving cows were stratified by age, body weight (BW), body condition score (BCS), and calf sex and allotted to 6 groups/yr. Groups were randomly assigned to 1 of 2 treatments: DL or PAST. Cows in DL were limit-fed at maintenance and calves had ad libitum access to the cow diet in an adjacent pen. Pairs on PAST were rotationally grazed and calves received creep ad libitum three weeks prior to weaning. On d 110 calves were fence-line weaned and behavior was observed on d 111 and 112. On d 116 calves were transported 272 kilometers to a feedlot for a 42-d receiving period. Behavior was evaluated again on d 117 and 118. Data were analyzed using the MIXED procedure of SAS except reproductive data which was analyzed using the GLIMMIX procedure. Cows on DL had greater (P ≤ 0.01) BW and BCS at weaning. There were no differences (P ≥ 0.42) detected in reproductive data. Cows on DL had greater (P = 0.02) milk production. Calves on DL had greater BW (P ≤ 0.01) on d 55 and at weaning and greater preweaning average daily gain (ADG). There were treatment × time effects (P = 0.01) for lying and eating on d 111 and 112. More DL calves were eating in the morning and lying in the evening. More (P < 0.01) PAST calves were walking on d 111. Pasture calves vocalized more (P ≤ 0.01) on d 112. On d 117, more (P ≤ 0.05) pasture calves were lying and eating, and DL vocalized more. On d 118, treatment × time and treatment effects were detected (P ≤ 0.02) for lying and walking. More PAST calves were lying and more DL calves were walking. Drylot calves had greater (P ≤ 0.02) BW at the beginning and end of the receiving phase. Pasture calves had greater (P < 0.01) ADG and tended (P = 0.10) to have greater gain efficiency during feedlot receiving phase. In conclusion, housing cow-calf pairs in drylots improved BW, BCS, and milk production of cows but did not affect reproductive performance. Drylot calves had increased BW and ADG during the preweaning phase. Calf behavior at weaning and receiving was influenced by preweaning housing. Pasture calves had improved receiving phase ADG and feed efficiency but were still lighter than drylot calves after 42 d receiving phase.

scholarly journals 197 Gene-regulation control of muscle fatigue during transportation with rumen-protected methionine supplementation

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 28-28
Author(s):  
Gaston F Alfaro ◽  
Sonia J Moisá ◽  
Soren P Rodning ◽  
Taylor E Novak
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fatigue ◽  
Messenger Rna ◽  
Control Diet ◽  
Average Daily Gain ◽  
Adaptation Period ◽  
Production Chain ◽  
Transportation Simulation ◽  
Gluten Feed ◽  
Dietary Treatments

Abstract Different participants generally located distant among them compose the U.S. beef production chain, therefore shipping is required for cattle at least once in their lifespan. The aim of this study was to determine the effect of Rumen-Protected Methionine (RPM) supplementation on muscle fatigue gene network, creatine synthesis (CKM), and Reactive Oxygen Species (ROS) metabolism after a transportation simulation in a 1.7-miles oval test track. Angus heifers (n = 18) were stratified by body weight (408 ± 64 kg; BW) and randomly assigned to dietary treatments: 1) control diet (CTRL), and 2) control diet + rumen-protected methionine (RPM). After a successful adaptation period to Calan gates, animals received a common diet of Bermuda hay ad libitum and a soy hulls and corn gluten feed based supplement. After 45 days under supplementation, animals were loaded onto a 32 × 7 ft. trailer and transported for 22 hours. Skeletal muscle biopsies, BW and blood samples were obtained on day 0 (Baseline), 43 (Pre-transport, “PRET”), and 45 (Post-transport, “POST”). Heifer’s average daily gain did not differ between Baseline and PRET (P = 0.41). Control heifer’s shrink was 8% of BW while RPM heifers shrink was 10% (P = 0.29). Cortisol level decreased after transportation, but no differences were observed between treatments (P = 0.94). Messenger RNA was extracted from skeletal muscle tissue and gene expression analysis was performed by RT-qPCR. Results showed that AHCY (Creatine synthesis pathway), SSPN (Sarcoglycan complex), DNMT3A (DNA Methylation), and SOD2 (Oxidative Stress-ROS) were upregulated (P < 0.05) in CTRL between baseline and PRET and, decreased between PRET and POST (P < 0.05) while they remained constant for RPM. Furthermore, CKM was not affected by treatments (P = 0.11). In conclusion, muscle fatigue related genes were not affected by RPM. Although, RPM could affect ROS production, after a long-term transportation.

Supplementing 2 g per day bovine lactoferrin from late gestation until weaning did not improve lactation performance of mixed parity sows

2019 ◽  
Vol 59 (12) ◽  
pp. 2191 ◽  
Author(s):  
Fan Liu ◽  
Jordon Hogg ◽  
Susie Kracht ◽  
Chris J. Brewster ◽  
Dave J. Henman ◽  
...  
Keyword(s):  
Survival Rate ◽  
Control Diet ◽  
Average Daily Gain ◽  
Late Gestation ◽  
Commercial Application ◽  
Bovine Lactoferrin ◽  
Lactation Performance ◽  
Large White ◽  
Litter Weight ◽  
Dietary Treatments

Context Lactoferrin is a non-haem binding protein that possesses antimicrobial, antioxidant and anti-inflammatory properties. A previous study showed lactoferrin supplementation from early gestation to weaning improved lactation performance in primiparous sows; however, it is unknown whether the supplementation within a more specified duration (from late gestation to weaning) can improve lactation performance in multiparous sows, which limits its commercial application. Aims This experiment investigated the effects of 2 g/day bovine lactoferrin supplementation from late gestation to weaning on lactation performance in mixed parity sows. Methods Forty-seven primiparous sows and 167 multiparous sows (up to parity 5; Large White × Landrace, PrimeGro Genetics, Corowa, NSW) were allocated to either a Control diet (2 g/day casein) or Lactoferrin supplement diet (2 g/day lactoferrin) when entering the farrowing house in late gestation (101 ± 4.6 days, mean ± s.d.) balanced by parity (2.4 ± 1.80, mean ± s.d.). Sows were housed individually in farrowing crates and fed the experimental diets until weaning (26 ± 2.5 days, mean ± s.d.). Farrowing outcomes and lactation performance were recorded. Key results Lactoferrin supplementation did not affect the number of piglets born alive, number of stillborn piglets, number of mummified fetuses, the percentage of born-light piglets (≤1.1 kg) or piglet pre-fostering survival rate. Cross-fostering within the first 48 h standardised the litter size and litter weight between dietary treatments. Piglet pre-weaning survival rate, litter weight gain, average daily gain of piglets, or coefficient of variation of piglet growth rate was not affected by lactoferrin supplementation. Daily feed intake during lactation, bodyweight and backfat thickness of sows at weaning were similar between the dietary treatments. Conclusions Two grams per day lactoferrin supplementation from late gestation to weaning did not affect lactation performance in mixed parity sows. Implications The effectiveness of lactoferrin supplementation may depend upon the duration of supplementation, which should be optimised in future studies.

scholarly journals Effects of dietary live yeast supplementation on growth performance and biomarkers of metabolism and inflammation in heat-stressed and nutrient-restricted pigs

2021 ◽  
Author(s):  
Edith J Mayorga ◽  
Sara K Kvidera ◽  
Erin A Horst ◽  
Mohmmad Al-Qaisi ◽  
Carrie S McCarthy ◽  
...  
Keyword(s):  
Growth Performance ◽  
Control Diet ◽  
Average Daily Gain ◽  
Yeast Saccharomyces Cerevisiae ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Factor Alpha ◽  
Ad Libitum ◽  
Dietary Treatments ◽  
Live Yeast

Abstract Study objectives were to determine the effects of dietary live yeast (Saccharomyces cerevisiae strain CNCM I-4407; ActisafHR+; 0.25g/kg of feed; Phileo by Lesaffre, Milwaukee, WI) on growth performance and biomarkers of metabolism and inflammation in heat-stressed and nutrient-restricted pigs. Crossbred barrows (n = 96; 79 ± 1 kg body weight [BW]) were blocked by initial BW and randomly assigned to 1 of 6 dietary-environmental treatments: 1) thermoneutral (TN) and fed ad libitum the control diet (TNCon), 2) TN and fed ad libitum a yeast containing diet (TNYeast), 3) TN and pair-fed (PF) the control diet (PFCon), 4) TN and PF the yeast containing diet (PFYeast), 5) heat stress (HS) and fed ad libitum the control diet (HSCon), or 6) HS and fed ad libitum the yeast diet (HSYeast). Following 5 d of acclimation to individual pens, pigs were enrolled in 2 experimental periods (P). During P1 (7 d), pigs were housed in TN conditions (20°C) and fed their respective dietary treatments ad libitum. During P2 (28 d), HSCon and HSYeast pigs were fed ad libitum and exposed to progressive cyclical HS (28 to 33°C) while TN and PF pigs remained in TN conditions and were fed ad libitum or PF to their HSCon and HSYeast counterparts. Pigs exposed to HS had an overall increase in rectal temperature, skin temperature, and respiration rate compared to TN pigs (0.3°C, 5.5°C, and 23 breaths per minute, respectively; P<0.01). During P2, average daily feed intake (ADFI) decreased in HS compared to TN pigs (30%; P<0.01). Average daily gain and final BW decreased in HS relative to TN pigs (P<0.01); however, no differences in feed efficiency (G:F) were observed between HS and TN treatments (P>0.16). A tendency for decreased ADFI and increased G:F was observed in TNYeast relative to TNCon pigs (P<0.10). Circulating insulin was similar between HS and TN pigs (P>0.42). Triiodothyronine and thyroxine levels decreased in HS compared to TN treatments (~19 and 20%, respectively; P<0.05). Plasma tumor necrosis factor-alpha (TNF-α) did not differ across treatments (P>0.57) but tended to decrease in HSYeast relative to HSCon pigs (P=0.09). In summary, dietary live yeast did not affect body temperature indices or growth performance and had minimal effects on biomarkers of metabolism; however, it tended to improve G:F under TN conditions and tended to reduce the proinflammatory mediator TNF-α during HS. Further research on the potential role of dietary live yeast in pigs during HS or nutrient restriction scenarios is warranted.

172 Effects of Dietary Valine, Isoleucine, and Tryptophan Supplementations to Diets Containing Excess Leucine from Corn Protein on Growth Performance of Growing Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 61-62
Author(s):  
Woongbi Bi Kwon ◽  
Jose A Soto ◽  
Hans H Stein
Keyword(s):  
Block Design ◽  
Control Diet ◽  
Average Daily Gain ◽  
Growing Pigs ◽  
Experimental Unit ◽  
Negative Effects ◽  
Final Body Weight ◽  
Corn Protein ◽  
Dietary Treatments ◽  
Daily Gain

Abstract An experiment was conducted to test the hypothesis that addition of Val, Ile, or Trp alone or in combination will reduce the negative effects of excess Leu in diets for growing pigs. A total of 288 growing pigs (28.6 ± 2.5 kg) were randomly assigned to 1 of 9 dietary treatments in a randomized complete block design. There were 2 barrows and 2 gilts in each pen and 8 replicate pens per treatment. A control diet based on corn and soybean meal and 8 diets based on corn and a high-protein corn product (48% crude protein) with 2 levels of crystalline L-Val (0 or 0.10%), L-Ile (0 or 0.10%), and L-Trp (0 or 0.05%) were formulated. The crystalline L-Val, L-Ile, and L-Trp increased standardized ileal digestible (SID) Val:Lys from 70 to 80%, SID Ile:Lys from 53 to 63%, and SID Trp:Lys from 18 to 23%, respectively. All diets were formulated to contain 1.00% SID Lys and the 8 diets containing corn protein contained 171% SID Leu:Lys. Individual pig weights were recorded at the beginning and at the conclusion of the 28-d experiment. Data were analyzed using the PROC MIXED of SAS with a pen as the experimental unit. Diet was the fixed effect and block and replicate within block were random effects. Results indicated that final body weight and average daily gain were not different between pigs fed the control diet and pigs fed the diet with Val and Trp addition, but greater (P < 0.001) than for pigs fed the diet with Val addition, Ile addition, Trp addition, Val and Ile addition, Ile and Trp addition, or Val, Ile, and Trp addition (Table 1). In conclusion, addition of Val and Trp to diets with excess Leu may prevent negative effects of excess Leu in diets for growing pigs.

Effect of Yeast Type and Dose on Growth Performance and Nutrient Digestibility of Growing Rabbits Fed Maize or Barley

2021 ◽  
Author(s):  
Amr M.A. Elmasry ◽  
Luis A. Miranda ◽  
Germán Mendoza ◽  
Fernando X. Plata
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Body Weight ◽  
Growth Performance ◽  
Control Diet ◽  
Average Daily Gain ◽  
Nutrient Digestibility ◽  
Feed Conversion ◽  
Final Body Weight ◽  
Positive Effects ◽  
Yeast Concentration

Background: The effects of the probiotics vary with the doses and strains used for this reason, a study was conducted over 35 days to determine the effect of live yeast supplementation in the diet of growing rabbits on growth performance and apparent nutrient digestibility.Methods: A total of 60 New Zealand and California rabbits (5 weeks old, average body weight 0.881±0.015 kg) were housed in 30 cages. They were fed with a control diet based on alfalfa and maize or barley grains and supplemented with Procreatin®7 (Saccharomyces cerevisiae CNCM I 1077) or Biosaf® SC47 (Saccharomyces cerevisiae NCYC Sc47) at three doses, namely 0, 6.4, or 12.8 ×109 CFU per kg basal diet.Result: The higher yeast concentration had a positive effect (P less than 0.01) on final body weight (BW), average daily gain (ADG) and carcass weight (CW). However, there were no significant differences in dry matter intake. As a result of this, the feed conversion ratio (FCR) decreased (P less than 0.01). Significant increases (P less than 0.05) in nutrient digestibility (DM, OM, NDF and ADF) were observed with both grains. The use of yeast in maize and barley-based diets of rabbits has positive effects on growth performance and nutrient digestibility.

scholarly journals PSV-1 Effects of the protein level on growth performance and carcass grading in fattening pig fed ad libitum

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 223-224
Author(s):  
Arnaud Samson ◽  
Claire Launay ◽  
Francesc Payola ◽  
Eric Schetelat ◽  
Hervé Fortune
Keyword(s):  
Growth Performance ◽  
Feed Intake ◽  
Average Daily Gain ◽  
Experimental Unit ◽  
Net Energy ◽  
Swine Industry ◽  
Synthetic Amino Acids ◽  
Growth Performances ◽  
Ad Libitum ◽  
Dietary Treatments

Abstract Reducing dietary crude protein (CP) with synthetic amino acids (AA) supplementation is beneficial for sustainable swine industry. However some discrepancies regarding the consequences on growth performance are observed. Therefore, this trial aimed to assess the effect of dietary CP level on growth performance of pigs from 35 to 113 kg BW. Growth performance of 63 pigs fed ad libitum and allocated to three groups (HH, MM and LL) was compared. Each group received one level of CP (‘H’ for High, ‘M’ for Medium and ‘L’ for Low) during growing (d0–d35) and finishing (d35–slaugther day) periods: 15.1%, 16.2%, 17.2% and 13.0%, 14.0%, 15.1% respectively. Diets were mostly based on wheat, corn and soybean meal. Within each feeding phase, diets were formulated to be similar in apparent total tract digestible phosphorus, net energy and standardized ileal digestible (SID) essential AA, the SID Lys levels being 0.95% and 0.78% during the growing and finishing period respectively. Data were analyzed using a linear model (R, 3.1.2) with individual as the experimental unit as feed intake, body weight and carcass grading were individually recorded. From d0 to d14 and from d35 to d63, average daily gain (ADG) and Growth:Feed ratio (G:F) were significantly reduced as the CP level decreased (P < 0.05) while average daily feed intake (ADFI) remained unaffected by the dietary treatments. From d14 to d35 and in late finishing period (d63-slaugther day), growth performance was not significantly affected by the dietary CP level. Over the 82-d of the experiment, G:F was significantly higher for ‘HH’ pigs (P < 0.01). Overall, ADG tended to be reduced as CP levels decreased (P = 0.10). There was no difference among treatments for parameters concerning carcass grading (lean meat percentage and meat yield). In conclusion, reducing CP levels while maintaining essential AA levels impaired growth performances of pigs.

Supplements containing Acacia karroo foliage reduce nematode burdens in Nguni and crossbred cattle

2009 ◽  
Vol 49 (8) ◽  
pp. 646 ◽  
Author(s):  
V. Xhomfulana ◽  
C. Mapiye ◽  
M. Chimonyo ◽  
M. C. Marufu
Keyword(s):  
Control Diet ◽  
Average Daily Gain ◽  
Crossbred Cattle ◽  
Leaf Meal ◽  
Acacia Karroo ◽  
Natural Pasture ◽  
Nguni Cattle ◽  
Sunflower Cake ◽  
Dietary Treatments ◽  
And Control

The objective of this study was to compare nematode worm burdens in Nguni and crossbred cattle supplemented with Acacia karroo leaf meal. Eighteen Nguni (274.4 ± 17.34 kg) and 12 crossbred (230.5 ± 14.62 kg) steers aged 19 months were allocated to three dietary treatments: A. karroo leaf meal, sunflower cake and control with no supplement. Steers in all the treatments grazed on natural pasture whereas those on A. karroo leaf meal and sunflower cake diets were given an additional 1.5 kg and 650 g of feed, respectively, to supply 150 g of protein per day for 60 days. Faecal samples collected from the steers’ recta every fortnight were examined for nematode egg types using the modified McMaster technique. At slaughter, the abomasa and intestines were collected, opened and washed for worm identification. Nguni steers supplemented with the A. karroo leaf meal diet had the lowest mean strongyles egg counts and mean total egg counts (P < 0.05). Steers that received the A. karroo leaf meal and sunflower cake diets had lower Haemonchus contortus and Oesophagostomum colombianum worm burdens than those that received the control diet (P < 0.05). Mean strongyles and Strongyloides egg counts, and H. contortus and Oes. colombianum worm counts were negatively correlated with average daily gain and body condition scores (P < 0.05). Generally, Nguni cattle supplemented with A. karroo leaf meal had the lowest egg loads and worm burdens. It was concluded that supplementing cattle with A. karroo could reduce nematode burdens.

scholarly journals Carcass traits and relative organ weights of growing rabbits fed graded levels of processed kola nut (Cola nitida) pod husks

2020 ◽  
Vol 46 (5) ◽  
pp. 112-119
Author(s):  
S. A. Adeyeye ◽  
J. O. Agbede ◽  
V. A. Aletor ◽  
O. D. Oloruntola ◽  
S. O. Ayodele ◽  
...  
Keyword(s):  
Body Weight ◽  
Deleterious Effect ◽  
Control Diet ◽  
Carcass Traits ◽  
Organ Weights ◽  
Ad Libitum ◽  
Dietary Treatments ◽  
Average Body ◽  
Cola Nitida ◽  
Average Body Weight

Carcass traits and relative organ weights of growing rabbits fed graded levels of processed (ash treated and rumen liquor fermented) kola nut (Cola nitida) pod husks (PKPH) were investigated. One hundred and twenty (120) weaner rabbits of mixed breeds (New-Zealand white × Chinchilla) of equal sexes and average body weight 525.46g were randomly allotted to four (4) dietary treatments at 0 (0.00 % PKPH), 10 (10 % PKPH), 20 (20 % PKPH), and 30 % (30 % PKPH) inclusion levels and designated as diets 1, 2, 3, and 4, respectively. Each treatment group was replicated ten (10) times with three (3) rabbits per replicate and was fed ad-libitum for 56 days. The highest slaughter weight (1813.80 g/r), dressing weight (1068.70 g/r) and dressing percentage (58.54 %) recorded in rabbits fed diet 3 (20% PKPH) were similar to those fed the control diet (0 % PKPH) and diet 2 (10% PKPH) but was significantly (P<0.05) higher than those fed diet 4 (30 % PKPH). The lungs, liver, kidney, heart, and spleen of the rabbits fed dietary treatments were not significantly influenced. It was concluded that processed kolanut pod husk had no deleterious effect on carcass and relative organs weight of weaner rabbits.

scholarly journals Standardized ileal digestibility of amino acids in canola meal fed to gestating and lactating sows1

2019 ◽  
Vol 97 (10) ◽  
pp. 4219-4226
Author(s):  
Deepak Ettungapladi Velayudhan ◽  
Manik M Hossain ◽  
Hans H Stein ◽  
C Martin Nyachoti
Keyword(s):  
Latin Square ◽  
Late Gestation ◽  
Canola Meal ◽  
Enzyme Complex ◽  
Ileal Digestibility ◽  
Lactating Sows ◽  
Ad Libitum ◽  
Enzyme Supplementation ◽  
Dietary Treatments ◽  
Collection Phase

Abstract An experiment was conducted to determine the standardized ileal digestibility (SID) of CP and AA in solvent extracted canola meal (CM) fed to gestating and lactating sows without or with a multi-enzyme complex. Eight sows cannulated on day 40 of gestation were randomly assigned to 1 of 4 dietary treatments in a replicated 4 × 4 Latin square design. The 4 diets included 2 cornstarch-based diets with 31.3% CM as the only source of AA, without or with a multi-enzyme complex, a casein-cornstarch diet to determine ileal endogenous AA losses, and a phosphorus-free diet (phosphorus digestibility data reported elsewhere). All diets contained 0.3% titanium dioxide. Gestating sows were fed 3.0 kg/d of the respective experimental diets, whereas, during lactation, sows had ad libitum access to experimental diets. Ileal digesta samples were collected in midgestation, late gestation, and lactation. In each period, after 6-d acclimation to the experimental diets, on days 7 and 8, ileal digesta samples were collected continuously for 12 h. Results indicated that sows in lactation had greater apparent ileal digestibility (AID) of CP and all AA (P < 0.05) compared with sows in gestation. Enzyme supplementation improved (P < 0.05) the AID of histidine, lysine, methionine, valine, and alanine, and a tendency (P < 0.10) for improvement in AID was observed for arginine, isoleucine, tryptophan, and cysteine during lactation, but not during gestation. However, the SID of most AA was not affected by collection phase, but enzyme supplementation improved (P < 0.05) the SID of arginine, histidine, lysine, methionine, and valine during lactation. The SID of indispensable AA in CM fed to gestating and lactating sows were as follows: arginine, 89.2 and 91.3%; histidine, 93.1 and 94.0%; isoleucine, 85.9 and 87.0%; leucine, 89.2 and 89.2%; lysine, 87.0 and 87.7%; methionine, 92.2 and 93.2%; phenylalanine, 89.2 and 87.8%; threonine, 84.3 and 82.7%; tryptophan, 88.1 and 91.5%; valine, 85.9 and 84.3%. In conclusion, the SID of AA in CM fed to lactating sows may be improved if a mixture of carbohydrases is included in the diet, but under the conditions of this experiment, the carbohydrase mixture did not affect SID of AA in CM fed to gestating sows in midgestation or late gestation.

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