scholarly journals Methods for Distinguishing Aspen Seedlings from Suckers in the Field

2020 ◽  
Vol 118 (6) ◽  
pp. 561-568
Author(s):  
Mark R Kreider ◽  
Karen E Mock ◽  
Larissa L Yocom

Abstract Quaking aspen is a common component of postdisturbance landscapes, in part because of its ability to regenerate via asexual suckers. Previously viewed as exceedingly rare in the western United States, sexual seedling establishment is increasingly seen as another important natural regeneration pathway for aspen, because sexual regeneration increases genetic diversity and facilitates long-distance dispersal. However, aspen seedling research is hampered by difficulties in visually distinguishing seedlings from suckers in the field, and few resources exist to guide managers and researchers. We present methods for distinguishing aspen seedlings from aspen suckers, suitable for use in field studies. Using these methods, we achieved 99 percent predictive accuracy in a recently burned area in southern Utah, although accuracy decreased to 90 percent following one summer’s growth, as seedlings and suckers became more similar in appearance. Study Implication: Sexual regeneration may play an important role in aspen’s response to climate change, as it increases genetic diversity and adaptive capacity, and allows aspen populations and their ecological associates to better track changing climate through increased dispersal distances. The methods we present allow managers and researchers to accurately identify aspen seedling populations, which can then be protected and monitored through time to better understand aspen demography and the factors limiting establishment and survival of new clones. Aspen seedling ecology is an area with many knowledge gaps currently, and these methods remove a barrier that has hindered research in the past.

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Zhigang Wu ◽  
Xinwei Xu ◽  
Juan Zhang ◽  
Gerhard Wiegleb ◽  
Hongwei Hou

Abstract Background Due to the environmental heterogeneity along elevation gradients, alpine ecosystems are ideal study objects for investigating how ecological variables shape the genetic patterns of natural species. The highest region in the world, the Qinghai-Tibetan Plateau, is a hotspot for the studies of evolutionary processes in plants. Many large rivers spring from the plateau, providing abundant habitats for aquatic and amphibious organisms. In the present study, we examined the genetic diversity of 13 Ranunculus subrigidus populations distributed throughout the plateau in order to elucidate the relative contribution of geographic distance and environmental dissimilarity to the spatial genetic pattern. Results A relatively low level of genetic diversity within populations was found. No spatial genetic structure was suggested by the analyses of molecular variance, Bayesian clustering analysis and Mantel tests. Partial Mantel tests and multiple matrix regression analysis showed a significant influence of the environment on the genetic divergence of the species. Both climatic and water quality variables contribute to the habitat heterogeneity of R. subrigidus populations. Conclusions Our results suggest that historical processes involving long-distance dispersal and local adaptation may account for the genetic patterns of R. subrigidus and current environmental factors play an important role in the genetic differentiation and local adaptation of aquatic plants in alpine landscapes.


2021 ◽  
pp. 1-10
Author(s):  
Zhiyue Zhao ◽  
Ning Zhao ◽  
Lide Fang ◽  
Xiaoting Li

During the long-distance transportation of wet-gas, the dominant frequency is of great significance for the study of pipeline fatigue and damage, and the safety production. Therefore, the theoretical and experimental researches for dominant frequency are carried out increasingly. However, most of the current prediction correlation of dominant frequency are mainly applicable to atmospheric pressure conditions (0.1 MPa), and the prediction accuracy is not accurate enough. The paper obtains the time series signal of liquid film thickness by near-infrared (NIR) sensor, and then calculates the wave frequency by the power spectrum density (PSD). The performance of typical predictive correlation is evaluated and analyzed by utilizing the experimental data at different flow and pressure conditions (0.1–0.8) MPa. The structure of Strouhal number and Lockhart-Martinelli (L-M) parameter are optimized reasonably, the mean velocity of the liquid film surface, the density increment of gas core, the gas core mass flow and average liquid film velocity are considered in the L-M parameter, a modified interfacial wave frequency correlation is proposed. The results indicate that the mean absolute error of the predictive correlation is 9.06% (current data) and 25.64% (literature data). The new correlation has a better predictive accuracy.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Agnieszka Sutkowska ◽  
Józef Mitka ◽  
Tomasz Warzecha ◽  
Jakub Bunk ◽  
Julia Rutkowska ◽  
...  

AbstractThe genetic diversity in 11 populations of Gladiolus imbricatus in five mountain ranges, including the Tatra, Pieniny, Gorce, Beskid Niski (Western Carpathians) and Bieszczady Mts (Eastern Carpathians), was studied with inter-simple sequence repeat (ISSR) markers. The species is a perennial plant occurring in open and semi-open sites of anthropogenic origin (meadows and forest margins). We checked a hypothesis on the microrefugial character of the plant populations in the Pieniny Mts, a small calcareous Carpathian range of complicated relief that has never been glaciated. Plant populations in the Tatra and Pieniny Mts had the highest genetic diversity indices, pointing to their long-term persistence. The refugial vs. the non-refugial mountain ranges accounted for a relatively high value of total genetic variation [analysis of molecular variance (AMOVA), 14.12%, p = 0.003]. One of the Pieniny populations was of hybridogenous origin and shared genetic stock with the Tatra population, indicating there is a local genetic melting pot. A weak genetic structuring of populations among particular regions was found (AMOVA, 4.5%, p > 0.05). This could be an effect of the frequent short-distance and sporadic long-distance gene flow. The dispersal of diaspores between the remote populations in the Western Carpathians and Eastern Carpathians could be affected by the historical transportation of flocks of sheep from the Tatra to Bieszczady Mts.


Heredity ◽  
2021 ◽  
Author(s):  
Yael S. Rodger ◽  
Alexandra Pavlova ◽  
Steve Sinclair ◽  
Melinda Pickup ◽  
Paul Sunnucks

AbstractConservation management can be aided by knowledge of genetic diversity and evolutionary history, so that ecological and evolutionary processes can be preserved. The Button Wrinklewort daisy (Rutidosis leptorrhynchoides) was a common component of grassy ecosystems in south-eastern Australia. It is now endangered due to extensive habitat loss and the impacts of livestock grazing, and is currently restricted to a few small populations in two regions >500 km apart, one in Victoria, the other in the Australian Capital Territory and nearby New South Wales (ACT/NSW). Using a genome-wide SNP dataset, we assessed patterns of genetic structure and genetic differentiation of 12 natural diploid populations. We estimated intrapopulation genetic diversity to scope sources for genetic management. Bayesian clustering and principal coordinate analyses showed strong population genetic differentiation between the two regions, and substantial substructure within ACT/NSW. A coalescent tree-building approach implemented in SNAPP indicated evolutionary divergence between the two distant regions. Among the populations screened, the last two known remaining Victorian populations had the highest genetic diversity, despite having among the lowest recent census sizes. A maximum likelihood population tree method implemented in TreeMix suggested little or no recent gene flow except potentially between very close neighbours. Populations that were more genetically distinctive had lower genetic diversity, suggesting that drift in isolation is likely driving population differentiation though loss of diversity, hence re-establishing gene flow among them is desirable. These results provide background knowledge for evidence-based conservation and support genetic rescue within and between regions to elevate genetic diversity and alleviate inbreeding.


2011 ◽  
Vol 25 (4) ◽  
pp. 937-940 ◽  
Author(s):  
Tânia Maria de Moura ◽  
Alexandre Magno Sebbenn ◽  
Karina Martins ◽  
Maria Andreia Moreno ◽  
Giancarlo Conde Xavier Oliveira ◽  
...  

This study aimed to compare the genetic diversity of populations of Solanum lycocarpum A.St.-Hil between natural and human disturbed environments, with the assumption that protected areas have greater genetic diversity than disturbed areas. For this study, two populations were sampled in Goiás State, Brazil. One was located in a conservation unit, Serra de Caldas Novas State Park, in the Caldas Novas municipality. The other was located in a pasture area in the municipality of Morrinhos. The two populations are 41 km apart. We sampled 60 individuals from each population, which were genotyped with five microsatellite loci (SSR). The highest number of alleles was recorded in the population of the conservation unit, where we found 11 exclusive and five rare alleles. In the disturbed area, we recorded only three exclusive alleles and one rare allele. Although we did not observe significant inbreeding in these populations, genetic divergence between them was high (G ST (Hedrick)=0.147 =0.147) for a species with long distance seed dispersal. The results corroborate the hypothesis that the population in the less disturbed area harbors greater allelic diversity. They also confirm the effectiveness of using protected areas to preserve the genetic diversity of the species.


2015 ◽  
Vol 33 (4) ◽  
pp. 946-958 ◽  
Author(s):  
Isabel Alves ◽  
Miguel Arenas ◽  
Mathias Currat ◽  
Anna Sramkova Hanulova ◽  
Vitor C. Sousa ◽  
...  

2022 ◽  
Vol 21 (2) ◽  
pp. 340-355
Author(s):  
Indrayuda Indrayuda ◽  
Mohd Effindi Samsuddin

This article reveals the changes in form and style of the Randai performance performed by the Minangkabau’s diaspora in Malaysia. The novelty of this research is the study of changes in forms and styles in Randai carried out by the Malaysian Minangkabau diaspora. The disconnection of socio-cultural communication and the long distance between Minangkabau and Peninsular Malaysia impacts the shift in forms and styles of Randai in the Minangkabau diaspora. This research was conducted qualitatively by designing research works such as pre-field studies, by collecting preliminary data based on a diffusion approach. In the field stage, the informants were selected by snowball sampling. The interview and observation instruments were designed based on the performance structure and performance procedures. Researchers conducted data analysis using the techniques recommended by Spradley, namely: (1) domain analysis, (2) taxonomic analysis, (3) componential analysis, and (4) discovering cultural themes. The study results revealed that the forms and styles were caused by mixing cultures between the diaspora and the Malaysian Malays. These changes were done so that the local community could accept Randai. In addition, Randai can be easily learned and cultivated by the Malay Malays and easily expressed by the Malaysian community and the Minangkabau diaspora as the perpetrators of Randai. These changes occur through a process of adaptation and acculturation. The implication of this research is the emergence of a new Randai model, namely Randai, which refers to local culture, both from the aspect of the story, Silat style, and music, as an identity and tradition for the Malaysian Minangkabau diaspora for the future.


Forests ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 1158 ◽  
Author(s):  
Cristina Zamora-Ballesteros ◽  
Julio J. Diez ◽  
Jorge Martín-García ◽  
Johanna Witzell ◽  
Alejandro Solla ◽  
...  

Fusarium circinatum (Nirenberg and O’ Donnell) is the causal agent of pine pitch canker (PPC) disease, one of the most devastating forest diseases worldwide. Long-distance spread occurs mainly through the movement of infected seeds whereas at regional level, the movement of seedlings, substrates, or containers may play an important role in fungal dispersal. Invasion of nurseries takes place via infected seeds and further spread can occur by planting contaminated seedlings, especially due to the possibility of infected plants remaining symptomless. Once established, F. circinatum spreads by rain, wind, and insects. The natural spread of the pathogen is limited due to the short dispersal distances of the spores and the fairly short flight distances of disseminating insects. In this review, we summarize the currently known dispersal pathways of the pathogen, discussing both natural and human-assisted processes. With the purpose of understanding how to best intervene in the disease’s development in nurseries and forests, we outline the epidemiology of the pathogen describing the key factors influencing its spread. Preventive measures to control the spread of F. circinatum locally and globally are described with special emphasis on the challenges in implementing them.


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