scholarly journals A rapid and convenient method for the preparation and storage of competent bacterial cells

1988 ◽  
Vol 16 (8) ◽  
pp. 3580-3580 ◽  
Author(s):  
C.T. Chung ◽  
Roger H. Miller
Keyword(s):  
Convenient Method ◽  
Bacterial Cells ◽  
And Storage

One-Step Preparation of Competent E. coli: Transformation and Storage of Bacterial Cells in the Same Solution

2021 ◽  
Vol 2021 (11) ◽  
pp. pdb.prot101212 ◽  
Author(s):  
Michael R. Green ◽  
Joseph Sambrook
Keyword(s):  
Escherichia Coli ◽  
Convenient Method ◽  
Plasmid Dna ◽  
Transformation Efficiency ◽  
Bacterial Cells ◽  
E Coli ◽  
One Step ◽  
And Storage

This protocol describes a convenient method for the preparation, use, and storage of competent Escherichia coli. The reported transformation efficiency of this method is ∼5 × 107 transformants/µg of plasmid DNA.

scholarly journals ‘Honeytrapping’ Pseudomonas From Water: A Sustainable Prototype For Water Disinfection

2021 ◽  
Author(s):  
Hemangi Ranade ◽  
Priya Paliwal ◽  
Debarati Paul ◽  
Manali Datta
Keyword(s):  
Potable Water ◽  
Cost Effective ◽  
Time Frame ◽  
Water Disinfection ◽  
Bacterial Cells ◽  
Log Reduction ◽  
And Storage ◽  
Subsequent Diffusion

Abstract This paper introduces a novel prototype for the removal of Pseudomonas from water samples. Bacterial cells have the tendency to get attracted towards specific chemicals (chemotaxis); a ‘honeytrap’ strip was conceptualized by integrating a combination of serine, pseudomonas specific chemo-attractant and honey to attract and inhibit the bacteria in situ. Honey, a natural antimicrobial agent, has garnered attention in its effective inhibitory role in Pseudomonal biofilms and wound infections. Dipping serine side of the strip attracted bacteria towards honeytrap, wherebythe porous nature of the strip facilitated the ‘trapping’ and subsequent diffusion of the bacterial cells towards honey-adsorbed end of the strip. This ‘honeytrap’ reportedly leads to the targeted elimination of Pseudomonas, hence facilitating its removal. The percentage efficacy of this ‘honeytrap’ device is 96% with a log reduction equivalent to 1.6 within a time frame of 2 hours. Pseudomonas aeruginosa, although, not a natural contaminant of potable water, enters circulation due to improperly maintained plumbing fixtures and storage facilities. Honeytrap strip is an easy to use, biodegradable and cost effective sustainable solution, and thus a scaled up version ofthis device may enablesubstantial improvement in quality of potable water.

scholarly journals Global Metabolomics Reveals That Vibrio natriegens Enhances the Growth and Paramylon Synthesis of Euglena gracilis

2021 ◽  
Vol 9 ◽  
Author(s):  
Ying Ouyang ◽  
Shuyu Chen ◽  
Liqing Zhao ◽  
Yiting Song ◽  
Anping Lei ◽  
...  
Keyword(s):  
Euglena Gracilis ◽  
Mass Production ◽  
Production Efficiency ◽  
Molecular Mechanisms ◽  
Dry Weight ◽  
Industrial Applications ◽  
Bacterial Cells ◽  
Cultivation System ◽  
Metabolic Interactions ◽  
And Storage

The microalga Euglena gracilis is utilized in the food, medicinal, and supplement industries. However, its mass production is currently limited by its low production efficiency and high risk of microbial contamination. In this study, physiological and biochemical parameters of E. gracilis co-cultivated with the bacteria Vibrio natriegens were investigated. A previous study reports the benefits of E. gracilis and V. natriegens co-cultivation; however, no bacterium growth and molecular mechanisms were further investigated. Our results show that this co-cultivation positively increased total chlorophyll, microalgal growth, dry weight, and storage sugar paramylon content of E. gracilis compared to the pure culture without V. natriegens. This analysis represents the first comprehensive metabolomic study of microalgae-bacterial co-cultivation, with 339 metabolites identified. This co-cultivation system was shown to have synergistic metabolic interactions between microalgal and bacterial cells, with a significant increase in methyl carbamate, ectoine, choline, methyl N-methylanthranilate, gentiatibetine, 4R-aminopentanoic acid, and glu-val compared to the cultivation of E. gracilis alone. Taken together, these results fill significant gaps in the current understanding of microalgae-bacteria co-cultivation systems and provide novel insights into potential improvements for mass production and industrial applications of E. gracilis.

Effect of Prestorage Treatments and Storage Conditions on the Survival of Salmonella Enteritidis PT4 and Listeria monocytogenes on Fresh Marine and Freshwater Aquaculture Fish

2004 ◽  
Vol 67 (1) ◽  
pp. 193-198 ◽  
Author(s):  
C. C. TASSOU ◽  
K. LAMBROPOULOU ◽  
G.-J. E. NYCHAS
Keyword(s):  
Listeria Monocytogenes ◽  
Salmonella Enteritidis ◽  
Pathogenic Bacteria ◽  
Modified Atmosphere Packaging ◽  
Storage Conditions ◽  
Hot Water ◽  
Bacterial Cells ◽  
Freshwater Aquaculture ◽  
Subsequent Storage ◽  
And Storage

The effect of prestorage treatments, such as immersion in a sorbate solution (5%, wt/vol), heating (60°C, 1 min), and a combination of the two treatments, and the subsequent storage in air or under modified atmosphere packaging (MAP; 40% CO2, 30% O2, and 30% N2) at chill temperatures (0 ± 1°C), on Listeria monocytogenes and Salmonella Enteritidis PT4 was studied. The prestorage treatments affected the pathogenic bacteria, and in all cases, there was a decrease in their population, with the sorbate and combination (hot water and sorbate) treatment being most effective. The beneficial effect of the prestorage treatments, which was more pronounced in storage under MAP conditions, suggests an interaction of the treatments with the CO2 of MAP against injured bacterial cells.

Characterization of Deoxynivalenol Detoxification by Lactobacillus paracasei LHZ-1 Isolated from Yogurt

2019 ◽  
Vol 82 (8) ◽  
pp. 1292-1299 ◽  
Author(s):  
YAOYAO ZHAI ◽  
SHANSHAN HU ◽  
LEI ZHONG ◽  
ZHAOXIN LU ◽  
XIAOMEI BIE ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Laser Scanning Confocal Microscopy ◽  
Lactobacillus Paracasei ◽  
Maximum Adsorption Capacity ◽  
Bacterial Cells ◽  
Scanning Confocal Microscopy ◽  
Binding Force ◽  
Viable Cells ◽  
And Storage

ABSTRACT Deoxynivalenol (DON) is a potent mycotoxin produced by many Fusarium spp. that invade grains during the growth and storage seasons. Lactic acid bacteria have been reported to be capable of removing several toxins, thereby providing an effective detoxification method for possible contaminated substrates. The present study mainly focused on investigating the detoxification characteristics of DON by a Lactobacillus paracasei LHZ-1 strain, which was recently isolated from yogurt with a strong promise of removing DON from liquid culture. The results obtained showed that the cell wall of L. paracasei LHZ-1 can remove up to 40.7% of 50 μg/mL DON, whereas only 10.5 and 8.9% are removed by the culture supernatant or cellular lysate, respectively. Laser scanning confocal microscopy helped to identify the mechanism of DON detoxification by L. paracasei LHZ-1 through cellular adsorption, where DON was found to bind to the surface of bacterial cells to form complexes. In stability tests, about 39 or 99% of bound DON, either to viable bacterial cells or heat-inactivated cells, respectively, was released by methanol extractions, which indicated that the binding force between viable cells and DON could be stronger than it is in heat-inactivated cells. Adsorption kinetics demonstrated that approximately 33% of DON was removed within 20 h, with a maximum adsorption capacity of approximately 50.5 μg/mL in phosphate-buffered solution.

An Overview of Digital Image Processing and Recognition (Invited Paper)

1982 ◽  
Vol 40 ◽  
pp. 700-702
Author(s):  
R. C. Gonzalez
Keyword(s):  
Image Processing ◽  
New York ◽  
Digital Image Processing ◽  
Digital Image ◽  
Practical Implementation ◽  
Submarine Cable ◽  
Vigorous Growth ◽  
Digitized Pictures ◽  
Processing Techniques ◽  
And Storage

Interest in digital image processing techniques dates back to the early 1920's, when digitized pictures of world news events were first transmitted by submarine cable between New York and London. Applications of digital image processing concepts, however, did not become widespread until the middle 1960's, when third-generation digital computers began to offer the speed and storage capabilities required for practical implementation of image processing algorithms. Since then, this area has experienced vigorous growth, having been a subject of interdisciplinary research in fields ranging from engineering and computer science to biology, chemistry, and medicine.

Effect of piperacillin on morphology and viability of gram-negative bacteria

1984 ◽  
Vol 42 ◽  
pp. 218-219
Author(s):  
R. H. Liss
Keyword(s):  
Inhibitory Concentration ◽  
Cytoplasmic Membrane ◽  
Drug Binding ◽  
Gram Negative Bacteria ◽  
Bacterial Cells ◽  
Drug Induced ◽  
Penicillin Binding Proteins ◽  
Lactam Antibiotic ◽  
Drug Free ◽  
Tube Dilution

Piperacillip (PIP) is b-[D(-)-α-(4-ethy1-2,3-dioxo-l-piperzinylcar-bonylamino)-α-phenylacetamido]-penicillanate. The broad spectrum semisynthetic β-lactam antibiotic is believed to effect bactericidal activity through its affinity for penicillin-binding proteins (PBPs), enzymes on the bacterial cytoplasmic membrane that control elongation and septation during cell growth and division. The purpose of this study was to correlate penetration and binding of 14C-PIP in bacterial cells with drug-induced lethal changes assessed by microscopic, microbiologic and biochemical methods.The bacteria used were clinical isolates of Escherichia coli and Pseudomonas aeruginosa (Figure 1). Sensitivity to the drug was determined by serial tube dilution in Trypticase Soy Broth (BBL) at an inoculum of 104 organisms/ml; the minimum inhibitory concentration of piperacillin for both bacteria was 1 μg/ml. To assess drug binding to PBPs, the bacteria were incubated with 14C-PIP (5 μg/0.09 μCi/ml); controls, in drug-free medium.

Thickness and composition determinations from T.E.M. specimens using both x-ray and backscattered electron data

1984 ◽  
Vol 42 ◽  
pp. 576-577
Author(s):  
M.D. Ball ◽  
H. Lagace ◽  
M.C. Thornton
Keyword(s):  
Electron Microscope ◽  
Atomic Number ◽  
Transmission Electron Microscope ◽  
Convenient Method ◽  
Flow Chart ◽  
X Ray ◽  
Intensity Measurements ◽  
Transmission Electron ◽  
Electron Intensity ◽  
Backscattered Electron

The backscattered electron coefficient η for transmission electron microscope specimens depends on both the atomic number Z and the thickness t. Hence for specimens of known atomic number, the thickness can be determined from backscattered electron coefficient measurements. This work describes a simple and convenient method of estimating the thickness and the corrected composition of areas of uncertain atomic number by combining x-ray microanalysis and backscattered electron intensity measurements.The method is best described in terms of the flow chart shown In Figure 1. Having selected a feature of interest, x-ray microanalysis data is recorded and used to estimate the composition. At this stage thickness corrections for absorption and fluorescence are not performed.

Control of Secretory Production in the Isopod Hepatopancreas

1972 ◽  
Vol 30 ◽  
pp. 166-167
Author(s):  
John W. Roberts ◽  
E. R. Witkus
Keyword(s):  
B Cells ◽  
Morphological Data ◽  
Secretory Product ◽  
Secretory Function ◽  
Secretory Material ◽  
Blind Ending ◽  
Secretory Production ◽  
Regenerative Cells ◽  
Immature Cells ◽  
And Storage

The isopod hepatopancreas, as exemplified by Oniscus ascellus. is comprised of four blind-ending diverticula. The regenerative cells at the tip of each diverticula differentiate into either club-shaped B-cells, which serve a secretory function, or into conoid S-cells, which serve in the absorption and storage of nutrients.The glandular B-cells begin producing secretory material with the development of rough endoplasmic reticulum during their process of maturation from the undifferentiated regenerative cells. Cytochemical and morphological data indicate that the hepatopancreas sequentially produces two types of secretory material within the large club-shaped cells. The production of the carbohydrate-like secretory product in immature cells seems to be phased out as the production of the osmiophilic secretion was phased in as the cell matured.

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