scholarly journals Development of FGF-2-loaded electrospun waterborne polyurethane fibrous membranes for bone regeneration

2020 ◽  
Author(s):  
Chi Zhang ◽  
Jianxiong Wang ◽  
Yujie Xie ◽  
Li Wang ◽  
Lishi Yang ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Periodontal Regeneration ◽  
Waterborne Polyurethane ◽  
Related Factors ◽  
Positive Meaning ◽  
Chain Extenders ◽  
Biomimetic Membrane ◽  
Fibrous Membranes ◽  
Green Chemical Synthesis ◽  
Gbr Membrane

Abstract Guided bone regeneration (GBR) membrane has been used to improve functional outcomes for periodontal regeneration. However, few studies have focused on the biomimetic membrane mimicking the vascularization of the periodontal membrane. This study aimed to fabricate waterborne polyurethane (WPU) fibrous membranes loaded fibroblast growth factor-2 (FGF-2) via emulsion electrospinning, which can promote regeneration of periodontal tissue via the vascularization of the biomimetic GBR membrane. A biodegradable WPU was synthesized by using lysine and dimethylpropionic acid as chain extenders according to the rule of green chemical synthesis technology. The WPU fibers with FGF-2 was fabricated via emulsion electrospinning. The results confirmed that controlled properties of the fibrous membrane had been achieved with controlled degradation, suitable mechanical properties and sustained release of the factor. The immunohistochemical expression of angiogenic-related factors was positive, meaning that FGF-2 loaded in fibers can significantly promote cell vascularization. The fiber scaffold loaded FGF-2 has the potential to be used as a functional GBR membrane to promote the formation of extraosseous blood vessels during periodontal repairing.

Antibacterial Properties of Guided Bone Regeneration Membrane for Periodontal Applications

2014 ◽  
Vol 606 ◽  
pp. 47-50
Author(s):  
Nur Najiha Saarani ◽  
K. Jamuna-Thevi ◽  
Ida Idayu Muhammad ◽  
Hendra Hermawan
Keyword(s):  
Staphylococcus Aureus ◽  
Bone Regeneration ◽  
Composite Membrane ◽  
Lauric Acid ◽  
Antibacterial Properties ◽  
Guided Bone Regeneration ◽  
Significant Inhibition ◽  
Thermally Induced ◽  
Zones Of Inhibition ◽  
Gbr Membrane

Guided Bone Regeneration (GBR) membrane is used as a barrier to prevent soft tissue ingrowth and to encourage bone regeneration through cellular exclusion. This study aims to assess antibacterial properties of recently developed three-layered poly(lactic-co-glycolic acid) (PLGA) /lauric acid (LA) composite membrane towards Staphylococcus aureus. One of the outmost layers of three-layered membrane was incorporated with 1-3 wt% of LA. The composite membrane was developed using thermally induced phase separation/solvent leaching technique. SEM results shows formation of PLGA matrix with smaller pores by the addition of 1 wt% LA compared with pure PLGA membrane. Samples of 1.7 cm diameter disk containing 1, 2 and 3 wt% of lauric acid were tested and pure membrane without lauric acid was used as a control. Results showed that the zones of inhibition were 2.3 cm and 2.5 cm for the 2 wt% and 3 wt% LA-containing membranes, respectively. However, 1 wt% LA-containing membrane observed to have no inhibition at all, indicating that increasing concentration of LA has significant inhibition against Staphylococcus aureus. The 3 wt% LA composition will be used in the mechanically optimized membranes for degradation studies in future works.

Tantalum-coated polylactic acid fibrous membranes for guided bone regeneration

2020 ◽  
Vol 115 ◽  
pp. 111112 ◽  
Author(s):  
Changha Hwang ◽  
Suhyung Park ◽  
In-Gu Kang ◽  
Hyoun-Ee Kim ◽  
Cheol-Min Han
Keyword(s):  
Bone Regeneration ◽  
Polylactic Acid ◽  
Guided Bone Regeneration ◽  
Fibrous Membranes

Characterization of Hydrophilized PCL Electrospun Sheet as an Efective Guided Bone Regeneration Membrane

2007 ◽  
Vol 342-343 ◽  
pp. 293-296 ◽  
Author(s):  
Wan Jin Cho ◽  
Jun Ho Kim ◽  
Se Heang Oh ◽  
Jin Ho Lee
Keyword(s):  
Mechanical Strength ◽  
Bone Regeneration ◽  
High Surface ◽  
High Surface Area ◽  
Tween 80 ◽  
Guided Bone Regeneration ◽  
Selective Permeability ◽  
Polymeric Biomaterial ◽  
Water Absorbability ◽  
Gbr Membrane

Electrospinning is a fabrication process that can produce highly porous nano-scale fiber-based matrices using an electrostatically driven jet of polymer solution. This method represents an attractive approach for polymeric biomaterial processing which provides the membrane structure that may retain mechanical strengths, flexibility, and high surface area. In this study, we prepared a guided bone regeneration (GBR) membrane with selective permeability, hydrophilicity, good mechanical strength and adhesiveness with bone using polycaprolactone (PCL) and Tween 80 by the electrospinning method. The prepared PCL and PCL/Tween 80 electrospun sheets were characterized via morphology observation, mechanical property, water absorbability, and model nutrient permeability. It was observed that the PCL/Tween 80 (3 wt%) electrospun sheet have an effective permeation of nutrients as well as the good mechanical strength to maintain a secluded space for the bone regeneration. From the results, the hydrophilized PCL/Tween 80 (3 wt%) electrospun sheet seem to be a good candidate as a GBR membrane.

Preparation of electrospun siloxane-poly(lactic acid)-vaterite hybrid fibrous membranes for guided bone regeneration

2010 ◽  
Vol 70 (13) ◽  
pp. 1889-1893 ◽  
Author(s):  
Takashi Wakita ◽  
Akiko Obata ◽  
Gowsihan Poologasundarampillai ◽  
Julian R. Jones ◽  
Toshihiro Kasuga
Keyword(s):  
Lactic Acid ◽  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Poly Lactic Acid ◽  
Fibrous Membranes

Preparation of Silicon-Containing Poly(lactic acid)-Vaterite Hybrid Membranes

2010 ◽  
Vol 638-642 ◽  
pp. 670-674
Author(s):  
Akiko Obata ◽  
Takashi Wakita ◽  
Yoshio Ota ◽  
Toshihiro Kasuga
Keyword(s):  
Lactic Acid ◽  
Simulated Body Fluid ◽  
Bone Regeneration ◽  
Trace Amount ◽  
Body Fluid ◽  
Poly Lactic Acid ◽  
Osteogenic Cells ◽  
A Cell ◽  
Silicon Doped ◽  
Gbr Membrane

Microfiber meshes releasing a trace amount of silicon species were prepared by electrospinning silicon-doped vaterite (SiV) and poly(lactic acid) (PLA) hybrids for application to membranes for guided bone regeneration (GBR). A trace amount of silicon-species has been reported to enhance the mineralization and bone-forming abilities of osteogenic cells. The microfiber meshes prepared by electrospinning are regarded to be a useful candidate for the GBR membrane, because they have adequate flexibility and porosity for it. In this study, hydroxyapatite (HA)-forming abilities in simulated body fluid, silicon-releasabilities, compatibility with osteoblast-like cells of the prepared microfiber meshes were examined. The meshes were completely coated with HA after soaking in simulated body fluid for 1 day. The meshes coated with HA released 0.2 -0.7 mg/L of silicon species in a cell culture medium for 7 days. The cells elongated on the microfibers of the meshes and some of them entered the mesh after 1 day-culturing. The meshes are expected to provide an excellent substrate for bone regeneration and enhance bone-forming ability of the cells.

scholarly journals ID: 1058 Effective bone formation through BMP-2-immobilized highly porous GBR membrane

2017 ◽  
Vol 4 (S) ◽  
pp. 139
Author(s):  
Min Ji Kim ◽  
Jin Hyun Park ◽  
Ho Yong Kim ◽  
June Ho Byun ◽  
Jin Ho Lee ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Bone Formation ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Active Sites ◽  
Healing Process ◽  
The Body ◽  
Bioactive Molecules ◽  
Bone Morphogenetic Protein 2 ◽  
Gbr Membrane

Sound healing of large bone defects is critical challenges in most of clinical fields. In general healing process of bone regeneration, the rapid infiltration of connective tissue, whereas relatively slow bone regeneration in bone defect leads to the incomplete bone formation. To solve this drawback, guide bone regeneration (GBR) membrane which could prevent rapid infiltration of connective tissue into bone defect, thus GBR membrane is feasible for compact bone regeneration in clinical fields. In recent, the most researchers believed that bioactive molecules-grafted GBR membranes may enhance the bone regeneration. To allow graft of bioactive molecules from porous membrane, chemically modified scaffolds are commonly used. This modification leads to sufficient interaction with active sites of bioactive molecules to stable the immobilization of bioactive molecules in the body. However it is hard to apply to clinical applications because of the toxicity of chemical residue used for the modification. In this study, we developed a GBR membrane with leaf-stacked structure which can allow sustained release of bone morphogenetic protein-2 (BMP-2) without any additional modification. The morphology, mechanical property, BMP-2 release profile, osteogenic differentiation of human periosteum-derived cells, and new bone formation efficiency of the BMP-2-loaded GBR membrane compared with commercial product were investigated.

scholarly journals New Waterborne Polyurethane-Urea Synthesized with Ether-Carbonate Copolymer and Amino-Alcohol Chain Extenders with Tailored Pressure-Sensitive Adhesion Properties

Materials ◽  
2020 ◽  
Vol 13 (3) ◽  
pp. 627 ◽  
Author(s):  
Mónica Fuensanta ◽  
Abbas Khoshnood ◽  
Francisco Rodríguez-Llansola ◽  
José Miguel Martín-Martínez
Keyword(s):  
Amino Alcohol ◽  
Peel Test ◽  
Waterborne Polyurethane ◽  
Chain Extender ◽  
Gravimetric Analysis ◽  
Scanning Calorimetry ◽  
Adhesion Properties ◽  
Oh Groups ◽  
Pressure Sensitive ◽  
Chain Extenders

New waterborne polyurethane-urea dispersions with adequate adhesion and cohesion properties have been synthesized by reacting isophorone diisocyanate, copolymer of ether and carbonate diol polyol and three amino-alcohols with different number of OH groups chain extenders using the prepolymer method. The waterborne polyurethane-urea dispersions were characterized by pH, particle-size distribution, and viscosity, and the polyurethane-urea films were characterized by attenuated total reflectance infrared (ATR-IR) spectroscopy, differential scanning calorimetry (DSC), thermal gravimetric analysis (TGA), and plate-plate rheology (temperature and frequency sweeps). Polyurethane-urea pressure-sensitive adhesives (PUU PSAs) were prepared by placing the waterborne polyurethane dispersions on polyethylene terephthalate (PET) films and they were characterized at 25 °C by creep test, tack and 180° peel test. The waterborne polyurethane-urea dispersions showed mean particle sizes between 51 and 78 nm and viscosities in the range of 58–133 mPa·s. The polyurethane-urea films showed glass transition temperatures (Tgs) lower than −64 °C, and they showed a cross of the storage and loss moduli between −8 and 68 °C depending on the number of OH groups in the amino-alcohol chain extender. Different types of PUU PSAs (removable, high shear) were obtained by changing the number of OH groups in the amino-alcohol chain extender. The tack at 25 °C of the PUU PSAs varied between 488 and 1807 kPa and the 180° peel strength values ranged between 0.4 and 6.4 N/cm, and their holding times were between 2 min and 5 days. The new PUU PSAs made with amino-alcohol chain extender seemed very promising for designing environmentally friendly waterborne PSAs with high tack and improved cohesion and adhesion property.

scholarly journals Synthesis and Characterization of Polycaprolactone-Based Polyurethanes for the Fabrication of Elastic Guided Bone Regeneration Membrane

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Shyh-Yuan Lee ◽  
Sheng-Chien Wu ◽  
Hsuan Chen ◽  
Lo-Lin Tsai ◽  
Jy-Jiunn Tzeng ◽  
...  
Keyword(s):  
Tensile Strength ◽  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Hexamethylene Diisocyanate ◽  
Mechanical And Thermal Properties ◽  
Elongation At Break ◽  
Tensile Tester ◽  
Electrospun Membranes ◽  
Chain Extenders

The aim of this research is to synthesize polycaprolactone-based polyurethanes (PCL-based PUs) that can be further used for the fabrication of guided bone regeneration (GBR) membranes with higher tensile strength and elongation at break than collagen and PTFE membranes. The PCL-based PUs were prepared by the polymerization of polycaprolactone (PCL) diol with 1,6-hexamethylene diisocyanate (HDI) at different ratios using either polyethylene glycol (PEG) or ethylenediamine (EDA) as chain extenders. The chemical, mechanical, and thermal properties of the synthesized polymers were determined using NMR, FTIR, GPC, DSC, and tensile tester. The PCL and polyurethanes were fabricated as nanofiber membranes by electrospinning, and their mechanical properties and SEM morphology were also investigated. In vitro tests, including WST-1 assay, SEM of cells, and phalloidin cytoskeleton staining, were also performed. It was shown that electrospun membranes made of PCL and PCL-HDI-PEG (2 : 3 : 1) possessed tensile strength of 19.84 MPa and 11.72 MPa and elongation at break of 627% and 362%, respectively. These numbers are equivalent or higher than most of the commercially available collagen and PTFE membrane. As a result, these membranes may have potential for future GBR applications.

scholarly journals Clinical Application of Mesenchymal Stem Cells and Novel Supportive Therapies for Oral Bone Regeneration

2015 ◽  
Vol 2015 ◽  
pp. 1-16 ◽  
Author(s):  
Miguel Padial-Molina ◽  
Francisco O’Valle ◽  
Alejandro Lanis ◽  
Francisco Mesa ◽  
David M. Dohan Ehrenfest ◽  
...  
Keyword(s):  
Stem Cells ◽  
Clinical Trials ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration ◽  
Case Reports ◽  
Periodontal Regeneration ◽  
Case Series ◽  
Bioactive Molecules ◽  
Alloplastic Materials ◽  
Clinical Scenarios

Bone regeneration is often needed prior to dental implant treatment due to the lack of adequate quantity and quality of the bone after infectious diseases, trauma, tumor, or congenital conditions. In these situations, cell transplantation technologies may help to overcome the limitations of autografts, xenografts, allografts, and alloplastic materials. A database search was conducted to include human clinical trials (randomized or controlled) and case reports/series describing the clinical use of mesenchymal stem cells (MSCs) in the oral cavity for bone regeneration only specifically excluding periodontal regeneration. Additionally, novel advances in related technologies are also described. 190 records were identified. 51 articles were selected for full-text assessment, and only 28 met the inclusion criteria: 9 case series, 10 case reports, and 9 randomized controlled clinical trials. Collectively, they evaluate the use of MSCs in a total of 290 patients in 342 interventions. The current published literature is very diverse in methodology and measurement of outcomes. Moreover, the clinical significance is limited. Therefore, the use of these techniques should be further studied in more challenging clinical scenarios with well-designed and standardized RCTs, potentially in combination with new scaffolding techniques and bioactive molecules to improve the final outcomes.

scholarly journals The Applications of Enamel Matrix Derivative in Implant Dentistry: A Narrative Review

Materials ◽  
2021 ◽  
Vol 14 (11) ◽  
pp. 3045
Author(s):  
Alice Alberti ◽  
Luca Francetti ◽  
Silvio Taschieri ◽  
Stefano Corbella
Keyword(s):  
Clinical Trials ◽  
Bone Regeneration ◽  
Randomized Clinical Trials ◽  
Periodontal Regeneration ◽  
Case Series ◽  
Titanium Implants ◽  
Enamel Matrix Derivative ◽  
Enamel Matrix ◽  
Implant Placement ◽  
Matrix Derivative

Enamel matrix derivative (EMD) has been successfully used for periodontal regeneration in intrabony defects. Recently, its use for peri-implant bone regeneration has also been hypothesized. The aim of this paper is to review preclinical and clinical studies investigating the use of EMD in correspondence with titanium implants, alone or as an adjunct to other biomaterials. Clinical trials and case series with more than five cases were included. Seven in vitro studies evaluated the effect of EMD, placed on titanium surfaces: An increase in proliferation and viability of osteoblasts was observed in all but two studies. An increase in TGF-β1 and osteocalcin production, alkaline phosphatase activity, and angiogenesis was also reported. Nine animal studies investigated the use of EMD at implant placement or for bone regeneration of peri-implant bone defects, and some of them reported a significant increase in bone formation or bone-to-implant contact. In four of eleven clinical trials on humans, EMD was successfully used at implant placement. The other seven evaluated the use of EMD in protocols for the treatment of peri-implantitis. In conclusion, the results of EMD seem promising, but further randomized clinical trials are needed to evaluate its efficacy.

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