Apparent total tract and ileal amino acids digestibility values of vegetal protein meals with dietary protease to broiler diet

Abstract This experiment was carried out to study the effect of dietary exogenous monocomponent protease on the coefficient apparent total tract (ATTD) and apparent ileal (AID) digestibility of amino acids of corn, soybean meal (SBM), and full fat soybean meal (FFSM) in broilers. A total of 400 males Cobb-500 (14 d of age) were equally allocated in 80 metabolic cages (50 cm × 50 cm × 45 cm) in a completely randomized design and a semi-controlled environment. Eight treatments (basal diet with or without a protease and three ingredients replacing the basal diets in 40% to corn and 30% to SBM and FFSM, with and without protease), with 10 replicates each were evaluated. The protease was added at 200 mg/kg resulting in 15,000 unit of PROT/kg. The total collection of excreta was held during 3 d, after 5 d for adaptation of broilers at the diets. The ileal content was collected on d 21, after slaughter of birds. The enzyme increased (P < 0.05) the ATTD and AID of most amino acids contained in SBM and FFSM. The digestibility of cysteine, glycine, proline, and threonine had higher (P < 0.05) ATTD and AID in all tested ingredients with the use of protease. On average, the dietary protease increased in 5.19% and 3.86% the total and ileal digestibility of amino acids, respectively. It was concluded that the dietary protease exerts major effects on toasted full-fat soybean, followed by soybean meal and corn to broilers.

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170 Energy values and digestibility of amino acids in dried yeast and soybean meal fed to pigs

Journal of Animal Science ◽

10.1093/jas/skaa054.109 ◽

2020 ◽

Vol 98 (Supplement_3) ◽

pp. 61-61

Author(s):

Chan Sol Park ◽

Ayodeji S Aderibigbe ◽

Gary Hayen ◽

Olayiwola Adeola

Keyword(s):

Amino Acids ◽

Soybean Meal ◽

Block Design ◽

Basal Diet ◽

Sole Source ◽

Metabolizable Energy ◽

Canola Meal ◽

Ileal Digestibility ◽

Randomized Complete Block Design ◽

Blocking Factors

Abstract Two experiments were conducted to evaluate the digestible energy (DE), metabolizable energy (ME), and standardized ileal digestibility (SID) of amino acids (AA) in dried yeast (DY) and soybean meal (SBM) fed to pigs. In Exp. 1, 30 barrows with an initial body weight (BW) of 20.7 ± 1.01 were assigned to 5 diets in a randomized complete block design with period and BW as blocking factors. A basal diet was prepared to contain corn, canola meal, and soybean oil as energy-contributing ingredients. Four additional diets were prepared by adding 5 or 10 g/kg DY or SBM at the expense of energy-contributing ingredients in the basal diet to estimate the DE and ME in test ingredients by regression analysis. On a dry matter basis, estimated DE and ME in DY were 4,022 and 3,352 kcal/kg, respectively, and those in SBM were 3,876 and 3,601kcal/kg, respectively. There was no difference in estimated DE or ME between DY and SBM. In Exp. 2, 21 barrows (initial BW = 20.0 ± 1.31 kg) surgically fitted with T-cannulas at the distal ileum were assigned to 3 diets in a randomized complete block design with BW as a blocking factor. Two diets were prepared to contain DY or SBM as the sole source of nitrogen, and a nitrogen-free diet was prepared to determine the basal ileal endogenous losses of AA. The SID of AA, except for Gly and Pro, in SBM were greater (P < 0.05) than in DY. The SID of indispensable AA in DY ranged from 64.7% for Thr to 86.1% for Arg, whereas those in SBM ranged from 84.8% for Thr to 92.3% for Arg. In conclusion, energy values in DY was comparable with SBM, but the SID of most AA in DY were less than in SBM.

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Amino acids digestibility of pelleted microparticle protein of fish meal and soybean meal in broiler chickens

Journal of the Indonesian Tropical Animal Agriculture ◽

10.14710/jitaa.43.2.169-176 ◽

2018 ◽

Vol 43 (2) ◽

pp. 169 ◽

Cited By ~ 2

Author(s):

N. Suthama ◽

P. J. Wibawa

Keyword(s):

Amino Acids ◽

Particle Size ◽

Soybean Meal ◽

Broiler Chickens ◽

Fish Meal ◽

Coconut Oil ◽

Intact Protein ◽

Calcium Retention ◽

Randomized Design ◽

Completely Randomized Design

Commom protein sources for poultry, fish meal and soybean meal, were ground to obtain reduced particle size. The particle was then dissolved in distilled water (1 : 4 w/v), and added with 2 mL virgin coconut oil for every 500 mL solution prior to ultrasound transducer (ultrasonic bath) treatment to obtain protein microparticle. Reducing particle size is one possible way to increase protein utilization.180 birds were used for forced feeding and 10 other birds were plotted for endogenous correction, when they were one month and a half old. Microparticle protein of both ingredients were tested separately in either mash or pelleted forms and compared to intact protein. Completely randomized design with 3 treatments (intact, mash, and pellet) and 6 replications (10 bidrs each) was arranged for the respective ingredient. Protein and essential amino acid digestibilities, and calcium retention were the parameters measured. Analysis of variance continued to Duncan test were applied to statistically evaluate the data. Pelleted microparticle protein of fish meal and soybean meal, respectively, resulted in significantly (P<0.05) highest protein and amino acids digestibilities, and Ca retention although lower disgestibility of fewer amino acids was found in mash form. In conclusion, pelleted form of microparticle protein of either fish meal or soybean meal improve protein and mostly amino acids digestibilities, and calcium retention in broiler.

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Apparent and standardised digestibility in broilers fed flaxseed meal with multicarbohydrase and phytase at 35 days of age

Canadian Journal of Animal Science ◽

10.1139/cjas-2021-0051 ◽

2021 ◽

Author(s):

Robert Guaracy Aparecido Cardoso Araujo ◽

Connie Gallardo Vela ◽

José Roberto Sartori ◽

Messias Alves Trindade Neto

Keyword(s):

Amino Acids ◽

Factorial Design ◽

Dry Matter ◽

Energy Use ◽

Ileal Digestibility ◽

Flaxseed Meal ◽

Randomized Design ◽

Calcium Phosphorus ◽

Completely Randomized Design ◽

To Receive

The apparent total tract digestibility (ATTD; trial 1) and the apparent (AID) and standardized (SID) ileal digestibility of the amino acids (AA) (trial 2) in flaxseed meal (FM) were evaluated with the addition of exogenous multi-carbohydrase (MC) and phytase (Phy). A total of 80 28-day-old broilers were allotted in a completely randomized design to receive treatments up to 35 days of age. A 2 × 2 factorial design as used to determine the enzyme effects, on the ATTD of dry matter, nitrogen, calcium, phosphorus and fibre; energy use and the AID and SID of AA, in five replicate cages. There was synergism between MC and Phy on the ATTD of nitrogen and energy. The isolated inclusion of MC and Phy on the ATTD of dry matter, calcium, phosphorus and fibre of FM. The enzyme inclusion on the AID and SID of AA in FM, established by comparing the means, suggested a better response to the addition of MC + Phy. Supplementation with Phy or combination of MC and Phy was a viable alternative to increase the ATTD of nutrients and energy. The Combination of MC + Phy resulted in higher apparent and standardised digestibility of amino acidsAA from FM.

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Canola meal on starting pigs feeding

Semina Ciências Agrárias ◽

10.5433/1679-0359.2015v36n6p3977 ◽

2015 ◽

Vol 36 (6) ◽

pp. 3977

Author(s):

Lina Maria Peñuela-Sierra ◽

Ivan Moreira ◽

Paulo Levi Oliveira Carvalho ◽

Juliana Beatriz Toledo ◽

Liliane Maria Piano Gonçalves ◽

...

Keyword(s):

Amino Acids ◽

Control Diet ◽

Basal Diet ◽

Canola Meal ◽

Experimental Unit ◽

Ileal Digestibility ◽

Randomized Design ◽

Protein Free Diet ◽

Performance Results ◽

Digestible Amino Acids

Three experiments were carried out to determine the nutritional values and evaluate the performance of piglets fed on canola meal. In experiment I, a digestibility assay was conducted using fourteen barrow pigs, with an initial body weight of 20.62±3.30 kg. The evaluated feedstuff was canola meal, with a level of 250 g/kg in the basal diet (corn + soybean meal-based). The experimental unit consisted of one pig, with a total of seven experimental units per diet. The values as (fed basis) of digestible (DE) and metabolizable (ME) energy of canola meal were 2,995 kcal/kg and 2,796 kcal/kg, respectively. In experiment II, ileal digestibility assays were carried out to determine the apparent and true ileal digestibility coefficient and digestible amino acids. Three crossbred pigs were used, with a BW of 38.6±1.98 kg. The treatments consisted of two diets, with a single source of protein (canola meal) and one protein-free diet (OFD). The values of digestible amino acids in canola meal were as follows: lysine: 11.8 g/kg; methionine+cystine: 9.1 g/kg; threonine: 7.9 g/kg; tryptophan: 2.4 g/kg; leucine: 15.7 g/kg; and isoleucine: 8.7 g/kg. In experiment III, 60 piglets (BW= 15.08±0.72 kg to 30.26±2.78 kg) were allotted in a completely randomized design. The treatments consisted of four diets with increasing levels of canola meal (50, 100, 150 and 200 g/kg), six replicates and experimental unit consisted of two pigs. Additionally, a control diet was formulated containing 0.0 g/kg CM. Regression analysis indicates that there was no effect (P?0.05) of the level of canola meal inclusion on pigs performance. The performance results suggest that it is feasible to use up to 200 g/kg of canola meal in starting pigs diet, without impairing performance and the feeding cost.

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Evaluation of Diets with Inclusion of Hydrothermal, Phytase, and Organic Acid Pretreated Canola Meal on Nutrient Digestibility in Swine

Sustainable Agriculture Research ◽

10.5539/sar.v9n2p41 ◽

2020 ◽

Vol 9 (2) ◽

pp. 41

Author(s):

Enkhjargal Darambazar ◽

Daalkhaijav Damiran ◽

Denise Beaulieu

Keyword(s):

Lactic Acid ◽

Organic Acid ◽

Basal Diet ◽

Nutrient Digestibility ◽

Canola Meal ◽

Ileal Digestibility ◽

Randomized Design ◽

Gross Energy ◽

Completely Randomized Design ◽

Phytate Content

The objective of this study was to evaluate the effects of dietary inclusion of hydrothermal, phytase, and organic acid pretreated canola meal (CM) on nutrient digestibility in swine. A basal diet barley, wheat, and CM based, was formulated. Four diets containing 30% hydrothermal pretreated CM with 50% moisture and 40°C, phytase (1000 FTU/kg activity), and either citric acid (CA), malic acid (MA), or lactic acid (LA), and a control containing pretreated CM without phytase or organic acid were fed to 12 ileal-cannulated finisher pigs (initial average BW 105.3 ± 2.7 kg) in a completely randomized design over two periods of 9 days per period. Nutrient composition and phytate content of the diets, ileal digesta, and feces were analyzed and apparent ileal and total tract digestibilities were determined. The inclusion of hydrothermal, phytase, and CA or MA pretreated CM in the diet decreased phytate P (by up to 38.6%) (p < 0.05), consequently increasing available P (by up to 55.6%). Apparent ileal digestibility (AID) was improved for P by 19.9 ‒ 35.1% units and apparent total tract digestibility of DM by 10.3 ‒ 14.8% units, of protein by 6.6 ‒ 12.1% units, and of gross energy by 12 ‒ 17% units across the treatments (p < 0.05), while AID of CP for MA treatment was up by 4.7% units (p < 0.05) relative to the control, indicating improved diet utilization, thus reduced excretion to the environment.

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Energy values and standardized ileal digestibility of amino acids in some feedstuffs for broilers

Semina Ciências Agrárias ◽

10.5433/1679-0359.2019v40n6p2651 ◽

2019 ◽

Vol 40 (6) ◽

pp. 2651

Author(s):

Felipe Santos Dalólio ◽

Diego Ladeira Silva ◽

Luiz Fernando Teixeira Albino ◽

Ricardo Vianna Nunes ◽

Valdir Ribeiro Junior ◽

...

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Control Diet ◽

Brown Rice ◽

Metabolizable Energy ◽

Experimental Unit ◽

Ileal Digestibility ◽

Randomized Design ◽

Completely Randomized Design ◽

Amino Acid Contents

The objective of this study was to determine the energy values, standardized ileal digestibility coefficients and digestible amino acid contents of parboiled brown rice bran (PBRB), protein sorghum meal (PSM) and bread waste meal (BWM) in broiler diets. In the first trial, 144 broiler chicks at 14 days of age were evaluated in a completely randomized design involving three feedstuffs + a control diet, with six replicates and six birds per experimental unit. The total excreta collection method was adopted to determine the energy values of the feedstuffs. In the second trial, 144 broilers at 24 days of age were used in a completely randomized design involving three feedstuffs + a protein-free diet, with six replicates and six birds per experimental unit. Ileal digesta was harvested to determine the standardized ileal digestibility coefficients and digestible amino acid contents of the feedstuffs. The nitrogen-corrected apparent metabolizable energy (AMEn) values of PBRB, PSM and BWM were 2638, 2799 and 3399 kcal.kg-1, respectively. The metabolizability coefficients of AMEn (CAMEn) of PBRB, PSM and BWM were 54.13, 57.35 and 86.66%, respectively. Bread waste meal had the highest CAMEn (p < 0.05) and standardized ileal digestibility of amino acids (p < 0.01), followed by PBRB and PSM. The main limiting digestible amino acids in PBRB, PSM and BWM were found at the following respective levels: methionine - 0.19, 0.33 and 0.24%; lysine - 0.40, 0.46 and 0.40%; threonine - 0.25, 0.50 and 0.45%; and valine - 0.44, 0.73 and 0.48%.

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Performance, gut morphometry and enzymatic activity of broilers fed neonatal supplementation in housing

Acta Scientiarum Animal Sciences ◽

10.4025/actascianimsci.v40i1.38423 ◽

2018 ◽

Vol 40 (1) ◽

pp. 38423

Author(s):

Tatiane Souza dos Santos ◽

Adriano Barbieri ◽

Robert Guaracy Aparecido Cardoso Araujo ◽

Gustavo Do Valle Polycarpo ◽

Daniela Felipe Pinheiro ◽

...

Keyword(s):

Amino Acids ◽

Polynomial Regression ◽

Pancreatic Enzyme ◽

Broiler Chicks ◽

Quadratic Effect ◽

Linear Effect ◽

Intestinal Length ◽

Randomized Design ◽

Relative Organ Weight ◽

Completely Randomized Design

The objective was to evaluate the performance, relative organ weight, morphometry, intestinal length and pancreatic enzyme activity of broilers receiving neonatal supplementation. A total of 900 Cobb 500 broiler chicks was housed in 30 boxes of 2.5 m². The treatments were divided in a completely randomized design with six replications, and consisted of five inclusion levels (0.0, 2.5, 5.0, 7.5 and 10 grams per bird). The supplement used was based of amino acids, vitamins and minerals, which was provided on the ration on the first day in the housing. Statistical analysis was performed using SAS (2008) and when significant broken down using a polynomial regression. Due to the inclusion of the neonatal supplement in the period from one to seven and one to 21 days of age, the feed intake presented a linear effect and the weight gain, a quadratic effect. The relative weights of the gizzard and liver showed a quadratic effect, and the inclusions of 4.18 and 3.41 grams/bird provided the lowest weights, respectively. The jejunum villi height decreased with supplementation as well as the activity of pancreatic lipase. It can be concluded that neonatal supplementation benefited the performance of birds up to 21 days of age.

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PSIV-21 Pellet processing reduces variability of corn soybean meal-based diet digestibility in growing pigs

Journal of Animal Science ◽

10.1093/jas/skaa054.310 ◽

2020 ◽

Vol 98 (Supplement_3) ◽

pp. 175-175

Author(s):

Élisabeth Chassé ◽

Frédéric Guay ◽

Marie-Pierre Létourneau-Montminy

Keyword(s):

Amino Acids ◽

Soybean Meal ◽

Crude Protein ◽

Dry Matter ◽

Crossover Design ◽

Growing Pigs ◽

Distal Ileum ◽

Ileal Digestibility ◽

Pelleted Diet ◽

The One

Abstract This study was conducted to determine the effect of pelleting on the digestibility of corn-soybean meal-based diet in growing pigs. Two trials with 6 pigs cannulated at the distal ileum were conducted. In each trial, pigs were assigned to each treatment following a crossover design. In each experiment, the same diet, composed of corn and soybean meal with 10% wheat from two different feed mills, was served in pellet or mash form. Pelleting allowed an increase in digestibility in one of the trials. Apparent ileal digestibility (AID) of dry matter (DM), crude protein (CP) and digestible energy (DE) were improved with pelleting by 8, 12 and 9% (P< 0.01). The AID of amino acids (AA) was also improved (P< 0.05). Apparent total tract digestibility (ATTD) was increased by pelleting in DM, CP and DE by 5, 7 and 6% respectively (P< 0.01). The digestibility of the mash diet in experiment 1 was lower than in the pelleted diet in the experiment 1 and both diets in experiment 2 as shown by the interaction Pelleting X Trial which was significant for the AID and ATTD of DM, CP and DE (P< 0.01). Therefore, in experiment 1, pelleting allows to improve the digestibility of diet to the same level as in experiment 2. The AID of CP was higher by 37% in the mash diet from the second experiment compared to the one in the first experiment. Even though the same ingredients were chosen in the two experiments, this shows the variability in digestibility existing between different feed mills and ingredient sources. This difference was not observed in pelleted diets. The results obtained in these two trials show that pelleting can reduce the variability of digestibility and then give a good digestibility of diets even if the ingredients are of different quality or sources.

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PSVI-13 Amino Acid Supplementation During the Adaptation Period Did Not Affect the Standardized Ileal Digestibility of Amino Acids in Corn and Soybean Meal Fed to Pigs

Journal of Animal Science ◽

10.1093/jas/skab054.327 ◽

2021 ◽

Vol 99 (Supplement_1) ◽

pp. 200-201

Author(s):

Hyunjun Choi ◽

Sun Jong You ◽

Beob Gyun G Kim

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Soybean Meal ◽

Latin Square ◽

Sole Source ◽

Adaptation Period ◽

Amino Acid Supplementation ◽

Ileal Digestibility ◽

Indispensable Amino Acids ◽

Dietary Treatments

Abstract The objective was to determine the influence of amino acid (AA) supplementation during the adaptation period on the ileal digestibility of crude protein and AA in corn and soybean meal (SBM). Six barrows with an initial body weight of 30.9 ± 2.6 kg fitted with a T-cannula in the distal ileum were assigned to a 6 × 6 Latin square design with 6 dietary treatments and 6 periods. Two experimental diets contained corn or SBM as the sole source of AA and an N-free diet was additionally prepared. For AA supplementation groups, an AA mixture consisted of Gly, Lys, Met, Thr, Trp, Ile, Val, His, and Phe was added to the corn diet and the N-free diet at the expense of cornstarch, and an AA mixture of Lys, Met, and Thr was added to the SBM diet. All diets contained 0.5% of chromic oxide. The 6 experimental diets were fed to the pigs for 4 and half days, and the 3 diets containing AA mixture were switched to the respective diets without AA mixture during the following 2 and half days. Ileal digesta were collected during the last 2 days. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Arg and Trp in corn (P < 0.05), but did not affect that in SBM. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Pro and Gly regardless of feed ingredient (P < 0.05), but did not affect that of other AA. All AA except Pro in corn and SBM were unaffected by the addition of AA mixture during the adaptation period. In conclusion, the addition of amino acid during the adaptation period does not affect the standardized ileal digestibility of indispensable amino acids in feed ingredients.

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Changes in Productive Performance, Blood Metabolites and Hematological Parameters of Growing Lambs Supplemented with Two Sources of Choline

Indian Journal of Animal Research ◽

10.18805/ijar.b-1385 ◽

2021 ◽

Author(s):

J.A. Martínez-García ◽

J.C. Garcia-Lopez ◽

P.A. Hernández-García ◽

G.D. Mendoza-Martínez ◽

A. Vázquez-Valladolid ◽

...

Keyword(s):

Hematological Parameters ◽

Live Weight ◽

Basal Diet ◽

Blood Metabolites ◽

Metabolic Functions ◽

Randomized Design ◽

Dark Color ◽

Completely Randomized Design ◽

Oral Doses ◽

Daily Gain

Background: Choline is a nutrient with numerous metabolic functions, but its requirements for ruminants are unknown. The supplementation with bypass choline could enhance productivity. Methods: Twenty-four male lambs (Rambouillet 23.5 kg±3.17 kg initial BW) were fed a basal diet with treatments which consisted of a control and oral doses of ruminally-protected choline (4 g/d RPC) and plant-based choline (4 g/d Biocholine) in a completely randomized design with initial weight as a covariate. The experiment was conducted for 42 days during which live weight, dry matter intake, carcass characteristics, blood metabolites and basic hemograms were measured. Result: The daily gain in lambs was similar between treatments. Intake was higher in lambs given Biocholine (1.32 kg/d). The L* (represents the light to dark color) value and mineral content in the meat were improved with both sources of choline. Blood triglycerides increased by RPC compared with the other treatments and cholesterol was reduced by Biocholine. Alanine transaminase (ALT) and aspartate aminotransferase (AST) activity decreased by effect of choline. Hematological parameters were affected by choline supplementation regardless of the source; erythrocyte, monocytes and lymphocytes count decreased with both sources of choline in growing lambs.

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