scholarly journals Profiling the Lolium perenne Microbiome: From Seed to Seed

2020 ◽  
Vol 4 (3) ◽  
pp. 281-289 ◽  
Author(s):  
Ian Tannenbaum ◽  
Jatinder Kaur ◽  
Ross Mann ◽  
Timothy Sawbridge ◽  
Brendan Rodoni ◽  
...  

Lolium perenne (perennial ryegrass) is a common temperate pasture grass species and is favored by dairy farmers. Commercially, the known association with the mutualistic endophytic fungus Epichloë festucae subsp. lolii is used to enhance insect resistance of host plants. Knowledge of other members of the microbiome and their functions are limited. Sequencing of the V4 region of the 16S ribosomal RNA gene was used to examine the bacterial microbiome of perennial ryegrass (Alto) seed (generation 1 [G1]), subsequent mature plants grown in soil and sand, and seed (generation 2 [G2]) from crosses of these mature plants. The G1 microbiome was dominated by the class Gammaproteobacteria. The mature plant microbiomes were far more diverse, comprising up to 37 classes inclusive of Gammaproteobacteria. Different growth media yielded different microbiome profiles in mature plants. The G2 microbiome, similar to the G1 microbiome, was dominated by Gammaproteobacteria as the primary constituent, with additional supplementation from class Bacilli. This suggests the continuation of a core microbiome which persists from seed through plant maturation to seed. This study sheds new light on the hereditability of perennial ryegrass bacterial microbiomes and has identified some operational taxonomic units of potential commercial significance due to their seed transmissibility, and their roles are currently being explored.

2004 ◽  
Vol 55 (4) ◽  
pp. 389 ◽  
Author(s):  
K. V. Cunliffe ◽  
A. C. Vecchies ◽  
E. S. Jones ◽  
G. A. Kearney ◽  
J. W. Forster ◽  
...  

Ryegrass species are among the most important species in sown pastures, turf settings, and weed populations worldwide. Perennial ryegrass (Lolium perenne L.) is an outcrossing, wind-pollinated grass. Recent research has demonstrated the feasibility of developing transgenic perennial ryegrass varieties. In order to model the consequences of gene flow from transgenic grass genotypes in a field situation, the model non-transgenic trait of fertility among autotetraploid genotypes was chosen. Gene flow over distance and direction from a donor plot to surrounding sexually compatible recipient plants was studied. Reproductive isolation was achieved through the fertility barrier that arises between tetraploid and diploid ryegrass genotypes, despite the presence of diploid plants in a meadow situation. Fertility was used as an indication of effective gene flow over distance and direction. Measures of the fertility of recipient plants included total seed production (TSP), floret site utilisation (FSU), and relative fertility of recipient plants as a percentage of those within the donor plot (RF%). A leptokurtic distribution for gene flow was identified, with differences in the rate of decline over distance depending on direction. Simple sequence repeat (SSR) polymorphism was used to identify the paternity of progeny plants. The proportional representation of parents among the progeny was not significantly different from that expected due to the numerical representation of the different donor parent genotypes. The results of this research will have important implications for risk analysis prior to the field release of transgenic ryegrasses, fescues, and other pasture grass species, and for seed production in terms of cultivar purity and optimum isolation distance.


Genome ◽  
2017 ◽  
Vol 60 (12) ◽  
pp. 1086-1088 ◽  
Author(s):  
Hiroshi Shinozuka ◽  
Noel O.I. Cogan ◽  
German C. Spangenberg ◽  
John W. Forster

RNA-Seq methodology has been used to generate a comprehensive transcriptome sequence resource for perennial ryegrass, an important temperate pasture grass species. A total of 931 547 255 reads were obtained from libraries corresponding to 19 distinct tissue samples, including both vegetative and reproductive stages of development. Assembly of data generated a final filtered reference set of 48 713 contigs and scaffolds. The transcriptome resource will support whole genome sequence assembly, comparative genomics, implementation of genotyping-by-sequencing (GBS) methods based on transcript sampling, and identification of candidate genes for multiple biological functions.


2011 ◽  
Vol 15 ◽  
pp. 157-162
Author(s):  
G.D. Milne

Recent discussion about pasture persistence concentrates on pastures based on perennial ryegrass, the most commonly used grass species. This paper raises the question as to whether some of the causes of poor pasture persistence are due to perennial ryegrass being used in environments to which it is not suited. The adaptation to environmental stresses, particularly water, temperature and nutrient deficiencies, in different regions of New Zealand of tall fescue, cocksfoot, phalaris, and lucerne are discussed, and how this impacts on persistence advantages over perennial ryegrass. Keywords: persistence, pasture, Dactylis glomerata, Festuca arundinacea, Lolium perenne, Medicago sativa, Phalaris aquatica


2021 ◽  
Vol 17 ◽  
Author(s):  
Tom Maxwell ◽  
Grant Edwards ◽  
Katherine Tozer ◽  
Gerald Cosgrove

Persistence is an important component of perennial pasture-grass productivity. Defining traits that affect persistence is essential for improving pasture longevity through plant breeding and for identifying persistence traits that should be included in cultivar ranking indices. Compared with conventional longitudinal studies, where a single sowing is monitored over time, repeated annual sowings allow the effects on persistence of sowing year and the ensuing interactions between environment and age of pasture to be identified. An experiment was commenced in 2015 under sheep grazing in Canterbury and in 2016 under cattle grazing in Waikato, where eight cultivars of perennial ryegrass representing different ploidy, flowering date, and cultivar age (release date), and one cultivar each of tall fescue and cocksfoot were sown in four randomised complete blocks in autumn each year. This paper reports interim data on spring and autumn pasture yield, composition, and density of 3-year-old, 2-year-old and 1-year-old pastures exposed to the same environmental conditions within the same, single year. There were significant effects on yield, botanical composition, basal cover and tiller density due to cultivar, pasture age, and their interaction. When the confounding effect of year-to-year variation was removed by comparing each age cohort in the same year, the underlying differences among grass species and cultivars, and ages of pasture, is starting to reveal the nature of this influence on pasture persistence.


Agronomy ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1206
Author(s):  
Chinthaka Jayasinghe ◽  
Pieter Badenhorst ◽  
Joe Jacobs ◽  
German Spangenberg ◽  
Kevin Smith

Perennial ryegrass (Lolium perenne L.) is one of the most important forage grass species in temperate regions of Australia and New Zealand. However, it can have poor persistence due to a low tolerance to both abiotic and biotic stresses. A major challenge in measuring persistence in pasture breeding is that the assessment of pasture survival depends on ranking populations based on manual ground cover estimation. Ground cover measurements may include senescent and living tissues and can be measured as percentages or fractional units. The amount of senescent pasture present in a sward may indicate changes in plant growth, development, and resistance to abiotic and biotic stresses. The existing tools to estimate perennial ryegrass ground cover are not sensitive enough to discriminate senescent ryegrass from soil. This study aimed to develop a more precise sensor-based phenomic method to discriminate senescent pasture from soil. Ground-based RGB images, airborne multispectral images, ground-based hyperspectral data, and ground truth samples were taken from 54 perennial ryegrass plots three years after sowing. Software packages and machine learning scripts were used to develop a pipeline for high-throughput data extraction from sensor-based platforms. Estimates from the high-throughput pipeline were positively correlated with the ground truth data (p < 0.05). Based on the findings of this study, we conclude that the RGB-based high-throughput approach offers a precision tool to assess perennial ryegrass persistence in pasture breeding programs. Improvements in the spatial resolution of hyperspectral and multispectral techniques would then be used for persistence estimation in mixed swards and other monocultures.


2000 ◽  
Vol 40 (8) ◽  
pp. 1059 ◽  
Author(s):  
W. J. Fulkerson ◽  
J. F. M. Fennell ◽  
K. Slack

A grazing study was conducted, over a 3-year period (1997–99), on the subtropical north coast of New South Wales, Australia, to compare the yield of prairie grass (Bromus willdenowii cv. Matua), tall fescue (Festuca arundinacea cv. Vulcan) and perennial ryegrass (Lolium perenne cv. Yatsyn), on a well-drained red krasnozem soil at Wollongbar Agricultural Research Institute (WAI) and on a heavy clay soil at Casino. The effect of grazing interval (equivalent to the time taken to regrow 1.5, 2.5 or 4 leaves/tiller) in spring, and forage quality of prairie grass in winter and spring was also assessed. At both sites, the dry matter (DM) yields of prairie grass over the establishment year and in year 2 were significantly (P<0.001) higher than for the other 2 grass species (mean for 2 years over the 2 sites was 23.8, 8.9 and 7.7 t DM/ha for prairie grass, ryegrass and tall fescue, respectively). In year 3, there was no production of tall fescue or ryegrass at the WAI site while prairie grass produced 11.3 t DM/ha although this was obtained from natural seedling recruitment after the sward was sprayed with a herbicide in February of that year. At the Casino site, ryegrass and tall fescue still made substantial growth in year 3 (3.1 and 2.1 t DM/ha for ryegrass and tall fescue, respectively) but this was significantly below the yields of prairie grass (5.5 t DM/ha). More frequent grazing of prairie grass in spring (equivalent to 1.5 leaves/tiller of regrowth) led to significantly (P<0.05) less plants surviving summer and less seedling recruitment in the following autumn. The annual yield of the 1.5 leaf treatment was significantly (P<0.05) lower than the remaining treatments but only in the third year of the study. Analysis of prairie grass forage samples, taken in June (vegetative sward) and November (reproductive sward), gave magnesium values of less than 0.2% DM which is below the concentration found in ryegrass and that recommended for dairy cattle. The Ca : P and K : (Ca + Mg) ratios in prairie grass improved, as a forage for dairy cows, with regrowth time up to 5 leaves/tiller. Metabolisable energy remained constant with regrowth time in June at 10.8 MJ/kg DM but fell significantly in November from 10.7 MJ/kg DM, immediately post-grazing, to 9.2 MJ/kg DM at the 4.5 leaves/tiller stage of regrowth. In contrast to observations in ryegrass, the water-soluble carbohydrate content of forage samples of prairie grass taken in November showed a substantial increase with regrowth time to over 12% DM at the 3 leaves/tiller stage of regrowth. The high productivity and forage quality of prairie grass obtained over a 3-year period suggests this grass species could be a suitable temperate perennial grass for subtropical dairy pastures. An appropriately long grazing interval in spring seems critical to optimise plant survival over summer and for adequate seed set for seedling recruitment the following autumn. If summer weeds and/or grasses invade to a significant extent, the large seedbank of prairie grass provides the opportunity to spray out the pasture in summer and rely on seedling recruitment to establish a new sward in autumn. The forage quality of prairie grass in winter and spring is similar to perennial ryegrass but the magnesium levels are substantially lower and stock grazing this type of pasture for extended periods would need to be supplemented with this mineral.


1999 ◽  
Vol 7 ◽  
pp. 151-156
Author(s):  
D.E. Hume ◽  
M.P. Rolston ◽  
D.B. Baird ◽  
W.J. Archie ◽  
M.R. Marsh

Emergence of volunteer perennial ryegrass (Lolium perenne L.) from seed buried in soil may contribute to the ingress of ryegrass in newly sown pastures. To investigate this, ryegrass seed infected with fungal endophyte (Neotyphodium lolii) was buried in nylon bags under pasture at two depths and at two sites (Palmerston North, Lincoln) in early/mid-autumn 1998. Seed bags were removed from the soil at intervals over the course of one year to determine seed viability and presence of endophyte in seedlings. Viability of seed declined rapidly to be 10% 3 months after burial. Further decline in viability was less, so that 12 months after burial 4% of seeds were still viable. Endophyte viability also declined, from 58% infection of seedlings at the time of burial to 21% at 12 months. This was at a slower rate than the decline in seed viability and from what might have been predicted from seed storage experiments. Viability of seed buried at 10 cm was greater than that buried at 3 cm (e.g., means, 10% and 1% after 6 months, respectively). This has implications for cultivation practices before pasture establishment. Seed buried at Lincoln maintained higher viability than seed buried at Palmerston North (e.g., means, 6% and 4% after 6 months, respectively), which was associated with drier soil conditions at Lincoln. Survival of buried seed may therefore be of greater importance in summer-dry east coast regions, compared with moist west coast environments or in wet years. The significance of buried ryegrass seed will depend on the numbers involved, but after 12 months there were still viable seeds left in the soil and some of these were infected with endophyte. This is important for pastures sown with ryegrass that is free of endophyte or infected with a selected endophyte, and for slower establishing grass species such as tall fescue. Keywords: endophyte, endophyte survival, Lolium perenne, Neotyphodium lolii, perennial ryegrass, seed burial, seed survival


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jesús Rojas-Jaimes ◽  
David Lindo-Seminario ◽  
Germán Correa-Núñez ◽  
Benoit Diringer

AbstractTicks are arthropods that can host and transmit pathogens to wild animals, domestic animals, and even humans. The bacterial microbiome of adult (males and females) and nymph Rhipicephalus microplus ticks collected from a collared peccary, Pecari tajacu, captured in the rural area of Botijón Village in the Amazon region of Madre de Dios, Peru, was evaluated using metagenomics. The Chao1 and Shannon–Weaver analyses indicated greater bacterial richness and diversity in female ticks (GARH; 375–4.15) and nymph ticks (GARN; 332–4.75) compared to that in male ticks (GARM; 215–3.20). Taxonomic analyses identified 185 operational taxonomic units representing 147 bacterial genera. Of the 25 most prevalent genera, Salmonella (17.5%) and Vibrio (15.0%) showed the highest relative abundance followed by several other potentially pathogenic genera, such as Paracoccus (7.8%), Staphylococcus (6.8%), Pseudomonas (6.6%), Corynebacterium (5.0%), Cloacibacterium (3.6%), and Acinetobacter (2.5%). In total, 19.7% of the detected genera are shared by GARH, GARM, and GARN, and they can be considered as the core microbiome of R. microplus. To the best of our knowledge, this study is the first to characterize the microbiome of ticks collected from P. tajacu and to report the presence of Salmonella and Vibrio in R. microplus. The pathogenic potential and the role of these bacteria in the physiology of R. microplus should be further investigated due to the possible implications for public health and animal health in populations neighboring the habitat of P. tajacu.


Author(s):  
T.K. James ◽  
K.N. Tozer ◽  
A. Rahman

Yellow bristle grass (Setaria pumila) is a summer growing annual grass with a C4 photosynthetic pathway. It is now becoming a serious weed in pasture where it is highly competitive with perennial ryegrass (Lolium perenne)/white clover (Trifolium repens) and is avoided by stock when at the seed head stage. On severely infested farms, it is estimated to cause production losses in the order of $100 000/100 ha unit, mostly due to stock not grazing areas where the yellow bristle grass is seeding. Keywords: yellow bristle grass, Setaria pumila, pasture, grass weed


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