scholarly journals CD56‐expression in bone marrow or other relevant tissue samples related to histopathological diagnosis and clinical outcome in children

2006 ◽  
Vol 20 (4) ◽  
Author(s):  
Birgitte Groenkaer Toft ◽  
Lars Peter Ryder ◽  
Mette Klarskov Andersen ◽  
Birgitte Frederiksen Lausen ◽  
Bodil Laub Petersen
Keyword(s):  
Bone Marrow ◽  
Clinical Outcome ◽  
Histopathological Diagnosis ◽  
Tissue Samples ◽  
Cd56 Expression

scholarly journals Label-Free Rapid Separation and Enrichment of Bone Marrow-Derived Mesenchymal Stem Cells from a Heterogeneous Cell Mixture Using a Dielectrophoresis Device

Sensors ◽  
2018 ◽  
Vol 18 (9) ◽  
pp. 3007 ◽  
Author(s):  
Junya Yoshioka ◽  
Yu Ohsugi ◽  
Toru Yoshitomi ◽  
Tomoyuki Yasukawa ◽  
Naoki Sasaki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Gradient Method ◽  
Label Free ◽  
Rapid Separation ◽  
Isolation System ◽  
Tissue Samples ◽  
Cell Based Therapy

Bone marrow-derived mesenchymal stem cells (BMSCs) are an important cell resource for stem cell-based therapy, which are generally isolated and enriched by the density-gradient method based on cell size and density after collection of tissue samples. Since this method has limitations with regards to purity and repeatability, development of alternative label-free methods for BMSC separation is desired. In the present study, rapid label-free separation and enrichment of BMSCs from a heterogeneous cell mixture with bone marrow-derived promyelocytes was successfully achieved using a dielectrophoresis (DEP) device comprising saw-shaped electrodes. Upon application of an electric field, HL-60 cells as models of promyelocytes aggregated and floated between the saw-shaped electrodes, while UE7T-13 cells as models of BMSCs were effectively captured on the tips of the saw-shaped electrodes. After washing out the HL-60 cells from the device selectively, the purity of the UE7T-13 cells was increased from 33% to 83.5% within 5 min. Although further experiments and optimization are required, these results show the potential of the DEP device as a label-free rapid cell isolation system yielding high purity for rare and precious cells such as BMSCs.

scholarly journals Role of CD138, CD56, and light chain immunohistochemistry in suspected and diagnosed plasma cell myeloma: A prospective study

2019 ◽  
Vol 08 (01) ◽  
pp. 60-64 ◽  
Author(s):  
Jasmita Dass ◽  
Sudheer Arava ◽  
Pravas Chandra Mishra ◽  
Amit Kumar Dinda ◽  
Hara Prasad Pati
Keyword(s):  
Bone Marrow ◽  
Image Analysis ◽  
Light Chain ◽  
Bone Marrow Aspirate ◽  
Plasma Cells ◽  
Diagnostic Value ◽  
Reactive Plasmacytosis ◽  
Cd56 Expression ◽  
A Prospective Study

Abstract Introduction: Plasma cells (PCs) have conventionally been counted on the bone marrow aspirate, and small focal involvement may be missed even on bone marrow biopsy sections. Material and Methods: We aimed to study the role of CD138, CD56, anti-κ, and anti-λ immunohistochemistry (IHC) to separate PC myeloma from reactive plasmacytosis and to study the utility of these in cases suspected as myelomas and lacking >10% PCs on bone marrow aspirate. The study comprised 35 diagnosed myelomas, 20 reactive plasmacytosis, and 19 M-band positive suspected myelomas. CD138 IHC was performed on all cases along with CD56, anti-κ, and anti-λ IHC. PCs were counted on CD138-immunostained sections by manual count and by image analysis. In addition, CD56 expression was correlated with clinical features in diagnosed myeloma group. Results: In all cases, both manual counts and image analysis, PC counts were significantly higher on the CD138 stained sections than bone marrow aspirates. It was seen that the manual PC counts and image analysis counts were equivalent in diagnosed myeloma cases. CD56 expression was seen in ~62.85% diagnosed myeloma cases while it was negative in cases of reactive plasmacytosis. CD56 expression was significantly higher in patients with lytic lesions (78.26% vs. 21.74%). CD138, anti-κ, and anti-λ IHC also helped classify 11/19 (57.8%) cases correctly. Conclusion: The use of CD138 along with the light chain and CD56 IHC adds a high diagnostic value in myeloma patients and suspected myeloma cases. The PCs can be counted manually on the CD138-immunostained sections and correlate well with the counts obtained by image analysis.

scholarly journals Acute myeloid leukemia–induced remodeling of the human bone marrow niche predicts clinical outcome

2020 ◽  
Vol 4 (20) ◽  
pp. 5257-5268
Author(s):  
Yiyang Chen ◽  
Lina Marie Hoffmeister ◽  
Yasmin Zaun ◽  
Lucas Arnold ◽  
Kurt Werner Schmid ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Acute Myeloid Leukemia ◽  
Survival Rate ◽  
Clinical Outcome ◽  
Myeloid Leukemia ◽  
Bone Marrow Niche ◽  
Dependent Manner ◽  
Hematopoietic Stem ◽  
Acute Myeloid ◽  
First Diagnosis

Abstract Murine models of myeloid neoplasia show how leukemia infiltration alters the hematopoietic stem cell (HSC) niche to reinforce malignancy at the expense of healthy hematopoiesis. However, little is known about the bone marrow architecture in humans and its impact on clinical outcome. Here, we dissect the bone marrow niche in patients with acute myeloid leukemia (AML) at first diagnosis. We combined immunohistochemical stainings with global gene expression analyses from these AML patients and correlated them with clinical features. Mesenchymal stem and progenitor cells (MSPCs) lost quiescence and significantly expanded in the bone marrow of AML patients. Strikingly, their HSC- and niche-regulating capacities were impaired with significant inhibition of osteogenesis and bone formation in a cell contact–dependent manner through inhibition of cytoplasmic β-catenin. Assessment of bone metabolism by quantifying peripheral blood osteocalcin levels revealed 30% lower expression in AML patients at first diagnosis than in non-leukemic donors. Furthermore, patients with osteocalcin levels ≤11 ng/mL showed inferior overall survival with a 1-year survival rate of 38.7% whereas patients with higher osteocalcin levels reached a survival rate of 66.8%. These novel insights into the human AML bone marrow microenvironment help translate findings from preclinical models and detect new targets which might pave the way for niche-targeted therapies in AML patients.

scholarly journals Clonal hematopoiesis in angioimmunoblastic T-cell lymphoma with divergent evolution to myeloid neoplasms

2020 ◽  
Vol 4 (10) ◽  
pp. 2261-2271 ◽  
Author(s):  
Natasha E. Lewis ◽  
Kseniya Petrova-Drus ◽  
Sarah Huet ◽  
Zachary D. Epstein-Peterson ◽  
Qi Gao ◽  
...  
Keyword(s):  
Bone Marrow ◽  
T Cell ◽  
Genetic Alterations ◽  
Divergent Evolution ◽  
Tissue Samples ◽  
Clonal Hematopoiesis ◽  
Follicular Helper ◽  
Myeloid Neoplasms ◽  
T Cell Lymphomas ◽  
Genomic Studies

Abstract TET2 and DNMT3A mutations are frequently identified in T-cell lymphomas of T follicular helper cell origin (TCL-TFH), clonal hematopoiesis (CH), and myeloid neoplasms (MNs). The relationships among these 3 entities, however, are not well understood. We performed comprehensive genomic studies on paired bone marrow and tissue samples as well as on flow cytometry–sorted bone marrow and peripheral blood subpopulations from a cohort of 22 patients with TCL-TFH to identify shared CH-type mutations in various hematopoietic cell compartments. Identical mutations were detected in the neoplastic T-cell and myeloid compartments of 15 out of 22 patients (68%), including TET2 (14/15) and DNMT3A (10/15). Four patients developed MNs, all of which shared CH-type mutations with their TCL-TFH; additional unique genetic alterations were also detected in each patient’s TCL-TFH and MN. These data demonstrate that CH is prevalent in patients with TCL-TFH and that divergent evolution of a CH clone may give rise to both TCL-TFH and MNs.

scholarly journals Somatic deletion of KDM1A/LSD1 gene is associated to advanced colorectal cancer stages

2019 ◽  
Vol 73 (2) ◽  
pp. 107-111 ◽  
Author(s):  
Ruth Ramírez-Ramírez ◽  
Melva Gutiérrez-Angulo ◽  
Jorge Peregrina-Sandoval ◽  
José Miguel Moreno-Ortiz ◽  
Ramon Antonio Franco-Topete ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Gene Deletion ◽  
In Situ Hybridisation ◽  
P Value ◽  
Histopathological Diagnosis ◽  
Fluorescence In Situ Hybridisation ◽  
Tissue Samples ◽  
Copy Numbers ◽  
Gene Copies ◽  
Advanced Stages

AimsKDM1A/LSD1 and ZNF217 are involved in a protein complex that participates in transcriptional regulation. ZNF217 has been analysed in numerous cancers and its amplification has been associated with advanced stages of disease; however, a similar role for KDM1A/LSD1 has not been uncovered. In this study, we estimated the number of KDM1A/LSD1 and ZNF217 gene copies in tissue samples from patients diagnosed with colorectal cancer (CRC), as well as its association with clinicopathological features in patients with CRC.MethodsParaffin-embedded tumour samples from 50 patients with CRC with a histopathological diagnosis of CRC were included. The number of copies of KDM1A/LSD1 and ZNF217 genes was determined by fluorescence in situ hybridisation (FISH). We also analysed the association between copy numbers of selected genes and clinicopathological data based on multivariate analysis.ResultsDeletion of the KDM1A/LSD1 gene occurred in 19 samples (38%), whereas ZNF217 gene amplification was identified in 11 samples (22%). We found a significant association between lymph node metastasis or advanced tumour stage and KDM1A/LSD1 gene deletion (p value=0.0003 and p value=0.011, respectively).ConclusionsKDM1A/LSD1 gene deletion could be considered a novel prognostic biomarker of late-stage CRC.

Abstract 4762: Deficient double strand breaks repair of bone marrow plasma cells correlates with better clinical outcome of multiple myeloma patients

2014 ◽  
Author(s):  
Maria Gkotzamanidou ◽  
Masood Shammas ◽  
Evangelos Terpos ◽  
Sathees C. Raghavan ◽  
Kenneth C. Anderson ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Bone Marrow ◽  
Clinical Outcome ◽  
Plasma Cells ◽  
Double Strand Breaks ◽  
Strand Breaks ◽  
Bone Marrow Plasma

Bone marrow miR-10a overexpression is associated with genetic events but not affects clinical outcome in acute myeloid leukemia

2018 ◽  
Vol 214 (1) ◽  
pp. 169-173 ◽  
Author(s):  
Ting-juan Zhang ◽  
Hong Guo ◽  
Jing-dong Zhou ◽  
Xi-xi Li ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Acute Myeloid Leukemia ◽  
Clinical Outcome ◽  
Myeloid Leukemia ◽  
Genetic Events ◽  
Acute Myeloid

scholarly journals Titanium pigment in tissues of drug addicts: report of 5 necropsied cases

2004 ◽  
Vol 59 (2) ◽  
pp. 86-88 ◽  
Author(s):  
Marcus Aurelho de Lima ◽  
Vitorino Modesto dos Santos ◽  
Samia Demachki ◽  
Javier Lazo
Keyword(s):  
Bone Marrow ◽  
Titanium Dioxide ◽  
Forensic Pathology ◽  
Drug Addicts ◽  
Potential Contribution ◽  
Tissue Samples ◽  
Polarized Microscopy ◽  
Body Tissues ◽  
Pathologic Findings ◽  
Morphologic Data

The aim of this report is to describe the anatomic-pathologic findings from necropsies of 5 drug addicts with titanium pigment in several organs after chronic intravenous injection of crushed propoxyphene hydrochloride tablets. Samples from liver, spleen, lungs, lymph nodes, and bone marrow were obtained, and after being grossly studied, they were submitted to evaluation using common light and polarized microscopy. In all 5 cases, a pigment with characteristics of titanium dioxide was found within tissue samples of the organs studied. Our findings suggest that research concerning titanium pigment within body tissues should be enhanced, considering the potential contribution of this morphologic data to forensic pathology.

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