scholarly journals Titanium pigment in tissues of drug addicts: report of 5 necropsied cases

2004 ◽  
Vol 59 (2) ◽  
pp. 86-88 ◽  
Author(s):  
Marcus Aurelho de Lima ◽  
Vitorino Modesto dos Santos ◽  
Samia Demachki ◽  
Javier Lazo
Keyword(s):  
Bone Marrow ◽  
Titanium Dioxide ◽  
Forensic Pathology ◽  
Drug Addicts ◽  
Potential Contribution ◽  
Tissue Samples ◽  
Polarized Microscopy ◽  
Body Tissues ◽  
Pathologic Findings ◽  
Morphologic Data

The aim of this report is to describe the anatomic-pathologic findings from necropsies of 5 drug addicts with titanium pigment in several organs after chronic intravenous injection of crushed propoxyphene hydrochloride tablets. Samples from liver, spleen, lungs, lymph nodes, and bone marrow were obtained, and after being grossly studied, they were submitted to evaluation using common light and polarized microscopy. In all 5 cases, a pigment with characteristics of titanium dioxide was found within tissue samples of the organs studied. Our findings suggest that research concerning titanium pigment within body tissues should be enhanced, considering the potential contribution of this morphologic data to forensic pathology.

scholarly journals Titanium Dioxide Presents a Different Profile in Dextran Sodium Sulphate-Induced Experimental Colitis in Mice Lacking the IBD Risk Gene Ptpn2 in Myeloid Cells

2021 ◽  
Vol 22 (2) ◽  
pp. 772
Author(s):  
Javier Conde ◽  
Marlene Schwarzfischer ◽  
Egle Katkeviciute ◽  
Janine Häfliger ◽  
Anna Niechcial ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Titanium Dioxide ◽  
Genetic Risk ◽  
Intestinal Inflammation ◽  
Sodium Sulphate ◽  
Food Additive ◽  
Wild Type ◽  
Dextran Sodium Sulphate ◽  
Risk Gene ◽  
The Impact

Environmental and genetic factors have been demonstrated to contribute to the development of inflammatory bowel disease (IBD). Recent studies suggested that the food additive; titanium dioxide (TiO2) might play a causative role in the disease. Therefore, in the present study we aimed to explore the interaction between the food additive TiO2 and the well-characterized IBD risk gene protein tyrosine phosphatase non-receptor type 2 (Ptpn2) and their role in the development of intestinal inflammation. Dextran sodium sulphate (DSS)-induced acute colitis was performed in mice lacking the expression of Ptpn2 in myeloid cells (Ptpn2LysMCre) or their wild type littermates (Ptpn2fl/fl) and exposed to the microparticle TiO2. The impact of Ptpn2 on TiO2 signalling pathways and TiO2-induced IL-1β and IL-10 levels were studied using bone marrow-derived macrophages (BMDMs). Ptpn2LysMCre exposed to TiO2 exhibited more severe intestinal inflammation than their wild type counterparts. This effect was likely due to the impact of TiO2 on the differentiation of intestinal macrophages, suppressing the number of anti-inflammatory macrophages in Ptpn2 deficient mice. Moreover, we also found that TiO2 was able to induce the secretion of IL-1β via mitogen-activated proteins kinases (MAPKs) and to repress the expression of IL-10 in bone marrow-derived macrophages via MAPK-independent pathways. This is the first evidence of the cooperation between the genetic risk factor Ptpn2 and the environmental factor TiO2 in the regulation of intestinal inflammation. The results presented here suggest that the ingestion of certain industrial compounds should be taken into account, especially in individuals with increased genetic risk

scholarly journals Label-Free Rapid Separation and Enrichment of Bone Marrow-Derived Mesenchymal Stem Cells from a Heterogeneous Cell Mixture Using a Dielectrophoresis Device

Sensors ◽  
2018 ◽  
Vol 18 (9) ◽  
pp. 3007 ◽  
Author(s):  
Junya Yoshioka ◽  
Yu Ohsugi ◽  
Toru Yoshitomi ◽  
Tomoyuki Yasukawa ◽  
Naoki Sasaki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Gradient Method ◽  
Label Free ◽  
Rapid Separation ◽  
Isolation System ◽  
Tissue Samples ◽  
Cell Based Therapy

Bone marrow-derived mesenchymal stem cells (BMSCs) are an important cell resource for stem cell-based therapy, which are generally isolated and enriched by the density-gradient method based on cell size and density after collection of tissue samples. Since this method has limitations with regards to purity and repeatability, development of alternative label-free methods for BMSC separation is desired. In the present study, rapid label-free separation and enrichment of BMSCs from a heterogeneous cell mixture with bone marrow-derived promyelocytes was successfully achieved using a dielectrophoresis (DEP) device comprising saw-shaped electrodes. Upon application of an electric field, HL-60 cells as models of promyelocytes aggregated and floated between the saw-shaped electrodes, while UE7T-13 cells as models of BMSCs were effectively captured on the tips of the saw-shaped electrodes. After washing out the HL-60 cells from the device selectively, the purity of the UE7T-13 cells was increased from 33% to 83.5% within 5 min. Although further experiments and optimization are required, these results show the potential of the DEP device as a label-free rapid cell isolation system yielding high purity for rare and precious cells such as BMSCs.

scholarly journals CD56‐expression in bone marrow or other relevant tissue samples related to histopathological diagnosis and clinical outcome in children

2006 ◽  
Vol 20 (4) ◽  
Author(s):  
Birgitte Groenkaer Toft ◽  
Lars Peter Ryder ◽  
Mette Klarskov Andersen ◽  
Birgitte Frederiksen Lausen ◽  
Bodil Laub Petersen
Keyword(s):  
Bone Marrow ◽  
Clinical Outcome ◽  
Histopathological Diagnosis ◽  
Tissue Samples ◽  
Cd56 Expression

TT virus has a ubiquitous diffusion in human body tissues: analyses of paired serum and tissue samples

2003 ◽  
Vol 10 (2) ◽  
pp. 95-102 ◽  
Author(s):  
T. Pollicino ◽  
G. Raffa ◽  
G. Squadrito ◽  
L. Costantino ◽  
I. Cacciola ◽  
...  
Keyword(s):  
Human Body ◽  
Tissue Samples ◽  
Tt Virus ◽  
Body Tissues ◽  
Paired Serum

scholarly journals Clonal hematopoiesis in angioimmunoblastic T-cell lymphoma with divergent evolution to myeloid neoplasms

2020 ◽  
Vol 4 (10) ◽  
pp. 2261-2271 ◽  
Author(s):  
Natasha E. Lewis ◽  
Kseniya Petrova-Drus ◽  
Sarah Huet ◽  
Zachary D. Epstein-Peterson ◽  
Qi Gao ◽  
...  
Keyword(s):  
Bone Marrow ◽  
T Cell ◽  
Genetic Alterations ◽  
Divergent Evolution ◽  
Tissue Samples ◽  
Clonal Hematopoiesis ◽  
Follicular Helper ◽  
Myeloid Neoplasms ◽  
T Cell Lymphomas ◽  
Genomic Studies

Abstract TET2 and DNMT3A mutations are frequently identified in T-cell lymphomas of T follicular helper cell origin (TCL-TFH), clonal hematopoiesis (CH), and myeloid neoplasms (MNs). The relationships among these 3 entities, however, are not well understood. We performed comprehensive genomic studies on paired bone marrow and tissue samples as well as on flow cytometry–sorted bone marrow and peripheral blood subpopulations from a cohort of 22 patients with TCL-TFH to identify shared CH-type mutations in various hematopoietic cell compartments. Identical mutations were detected in the neoplastic T-cell and myeloid compartments of 15 out of 22 patients (68%), including TET2 (14/15) and DNMT3A (10/15). Four patients developed MNs, all of which shared CH-type mutations with their TCL-TFH; additional unique genetic alterations were also detected in each patient’s TCL-TFH and MN. These data demonstrate that CH is prevalent in patients with TCL-TFH and that divergent evolution of a CH clone may give rise to both TCL-TFH and MNs.

B-chromosome Systems in the Greater Glider (Petauroides volans volans) (Marsupialia : Petauridae). I. B-chromosome Distribution

1985 ◽  
Vol 38 (1) ◽  
pp. 189 ◽  
Author(s):  
Leon R McQuade
Keyword(s):  
Bone Marrow ◽  
Chromosome Number ◽  
Y Chromosome ◽  
Stable Equilibrium ◽  
Bone Marrow Cells ◽  
B Chromosome ◽  
B Chromosomes ◽  
Diploid Chromosome Number ◽  
Body Tissues ◽  
Distinct Distribution

Variations in diploid chromosome number, due to the presence of B chromosomes, are found within the distribution of P. v. volans. B chromosomes vary in number between one and eight per animal, are mitotically stable in various body tissues and, unlike the Y chromosome in male P. v. volans, are not eliminated from bone marrow cells. Animals possessing B chromosomes have a distinct distribution, and it appears that a stable equilibrium between the forces of B chromosome accumulation or elimination is operating in those populations possessing these chromosomes.

A Simple but Effective Demineralizing Method for Osteogenic Allograft Preparation

2005 ◽  
Vol 288-289 ◽  
pp. 249-252
Author(s):  
Hong In Shin ◽  
H.M. Ryoo ◽  
Suk Young Kim
Keyword(s):  
Bone Formation ◽  
Preparation Method ◽  
Ectopic Bone Formation ◽  
Short Treatment ◽  
H2o2 Treatment ◽  
Tissue Samples ◽  
Light Microscopic Observation ◽  
Ectopic Bone ◽  
Pathologic Findings ◽  
Osteogenic Effect

Bone regeneration with demineralized bone preparations has demonstrated its potential in grafting procedures in surgical disciplines of both medicine and dentistry. To improve the effectiveness in preparation and osteogenic regeneration, we developed a simple and rapid demineralizing method for osteogenic allograft preparation and evaluated its osteogenic effect using an ectopic bone formation assay. The rat diaphyseal cortical bones were demineralized in heated 0.6N HCl at 60° C for 10 to 30 mins using a controlled-heat ultrasonic cleaner, washed with sterilized distill water, and dehydrated with graded alcohol after 30% H2O2 treatment. The prepared grafts were implanted in rat dorsal subcutaneous pouches for 1-3 weeks and then the harvested tissue samples were prepared for routine light microscopic observation. The allografts demineralized with 60°C HCl for more than 20 mins in an ultrasonic condition were completely demineralized and effectively induced ectopic formation without specific pathologic findings. These findings suggest that demineralization with 60°C HCl for around 20 mins using a controlled-heat ultrasonic cleaner and dehydration with graded alcohol after short treatment with 30% H2O2 is a very simple but effective osteogenic allograft preparation method with minimal antigenicity and sterilizing effects.

Sign in / Sign up

Export Citation Format

Share Document