Partial Liquid Ventilation Reduces Fluid Filtration of Isolated Rabbit Lungs with Acute Hydrochloric Acid-induced Edema

2001 ◽  
Vol 94 (6) ◽  
pp. 1045-1049 ◽  
Author(s):  
Stephan A. Loer ◽  
Jörg Tarnow
Keyword(s):  
Body Weight ◽  
Hydrochloric Acid ◽  
Intratracheal Instillation ◽  
Partial Liquid Ventilation ◽  
Liquid Ventilation ◽  
Lung Weight ◽  
Fluid Filtration ◽  
Group 4 ◽  
Group 3 ◽  
Group 2

Background Hydrochloric acid aspiration increases pulmonary microvascular permeability. The authors tested the hypothesis that partial liquid ventilation has a beneficial effect on filtration coefficients in acute acid-induced lung injury. Methods Isolated blood-perfused rabbit lungs were assigned randomly to one of four groups. Group 1 (n = 6) served as a control group without edema. In group 2 (n = 6), group 3 (n = 6), and group 4 (n = 6), pulmonary edema was induced by intratracheal instillation of hydrochloric acid (0.1 N, 2 ml/kg body weight). Filtration coefficients were determined 30 min after this injury (by measuring loss of perfusate after increase of left atrial pressure). Group 2 lungs were gas ventilated, and group 3 lungs received partial liquid ventilation (15 ml perfluorocarbon/kg body weight). In group 4 lungs, the authors studied the immediate effects of bronchial perfluorocarbon instillation on ongoing filtration. Results Intratracheal instillation of hydrochloric acid markedly increased filtration coefficients when compared with non-injured control lungs (2.3 +/- 0.7 vs. 0.31 +/- 0.08 ml.min(-1). mmHg(-1).100 g(-1) wet lung weight, P < 0.01). Partial liquid ventilation reduced filtration coefficients of the injured lungs (to 0.9 +/- 0.3 ml.min(-1).mmHg(-1).100 g(-1) wet lung weight, P = 0.022). Neither pulmonary artery nor capillary pressures (determined by simultaneous occlusion of inflow and outflow of the pulmonary circulation) were changed by hydrochloric acid instillation or by partial liquid ventilation. During ongoing filtration, bronchial perfluorocarbon instillation (5 ml/kg body weight) immediately reduced the amount of filtered fluid by approximately 50% (P = 0.027). Conclusions In the acute phase after acid injury, partial liquid ventilation reduced pathologic fluid filtration. This effect started immediately after bronchial perfluorocarbon instillation and was not associated with changes in mean pulmonary artery, capillary, or airway pressures. The authors suggest that in the early phase of acid injury, reduction of fluid filtration contributes to the beneficial effects of partial liquid ventilation on gas exchange and lung mechanics.

Defensive activity of Hesperidin against Ciprofloxacin induced hepatic injury in rabbits.

2019 ◽  
Vol 10 (03) ◽  
Author(s):  
Hawraa M. Murad ◽  
Tamadhur Hani Hussein ◽  
Audai Sulaiman Khudhair ◽  
Manal Muhi Murad ◽  
Jawad Kadhim Faris
Keyword(s):  
Body Weight ◽  
Bacterial Infections ◽  
Hepatoprotective Activity ◽  
The Body ◽  
Local Breed ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Defensive Activity ◽  
Antioxidant Effects

This study was conducted to find out hepatoprotective activity of hesperidin (HES) 100mg/kg body weight (b.w.) against ciprofloxacin (CPX) 100 mg/kg induced hepatotoxicity in local breed rabbits .CPX is a broad spectrum antibiotic used for treatment of many bacterial infections. Twenty four male rabbits were divided into four groups ,group1: control, (1 ml/kg Saline orally) group 2: CPX (100 mg/kg orally) for (14) consecutive days , group 3: HES (100 mg//kg) orally for (14) consecutive days group 4: CPX (100 mg/kg orally) plus HES (100 mg//kg orally ) for (14) consecutive days. All the rabbits were killed on the (15) day of the experiment, and then the blood, and livers samples were taken. CPX induced hepatotoxicity was proved by a significant (p less than 0.01) reduction in the body weight ,and a significant (p less than 0.01) increased serum aspartate transaminase (AST), alanine transaminase (ALT) , Malonaldehyde enzyme (MAD) and histopathological changes. Protective hepatic toxicity effect and oxidative damage caused by CPX significantly (p less than 0.01) increasing in body weight and significantly (p less than 0.01) decreasing AST , ALT, MAD and improving tissue morphology in HES (100 mg//kg) . These results assure that HES (100 mg//kg) antioxidant effects can protect CPX-induced hepatotoxicity in rabbits.

scholarly journals Comparative analysis of three different filling techniques and the effects of experimental internal resorptive cavities on apical microleakage

2014 ◽  
Vol 08 (01) ◽  
pp. 032-037 ◽  
Author(s):  
Ali Keles ◽  
Fuat Ahmetoglu ◽  
Mevlut S. Ocak ◽  
Burak Dayi ◽  
Alperen Bozkurt ◽  
...  
Keyword(s):  
Laser System ◽  
Root Canal Filling ◽  
Fluid Filtration ◽  
Group 4 ◽  
Gutta Percha ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Apical Leakage ◽  
Group 1

ABSTRACT Objectives: The aim of this study was to evaluate the sealing abilities of three different gutta-percha techniques in experimentally defective roots (EDR) and non-defective roots (NR). Materials and Methods: Sixty canine teeth were divided into six groups of ten; Group 1, NR + cold lateral condensation (CLC); Group 2, EDR + LC; Group 3, NR + BeeFill; Group 4, EDR + BeeFill; Group 5, NR + Thermafil; and Group 6, EDR + Thermafil. Apical leakage was measured using a computerized fluid filtration meter with a laser system. Results: Statistical analysis revealed that the CLC demonstrated more microleakage in the EDR than in the NR (P < 0.01). Thermafil demonstrated more microleakage in the NR than in the EDR (P < 0.01). No statistically significant differences were found between the BeeFill groups (P > 0.05). Conclusions: The results of this study indicate that internal resorptive cavities can affect the apical sealing properties of different root canal filling techniques, with Thermafil ensuring the lowest apical microleakage.

scholarly journals Evaluation of chemopreventive and chemotherapeutic effect of Artemisia vulgaris extract against diethylnitrosamine induced hepatocellular carcinogenesis in Balb C mice

2020 ◽  
Vol 80 (3) ◽  
pp. 484-496 ◽  
Author(s):  
S. Ali ◽  
M. Ejaz ◽  
K. K. Dar ◽  
S. Nasreen ◽  
N. Ashraf ◽  
...  
Keyword(s):  
Body Weight ◽  
Plant Extract ◽  
Control Group ◽  
Gamma Glutamyl Transferase ◽  
Artemisia Vulgaris ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Glutamyl Transferase ◽  
Glucose 6 Phosphate Dehydrogenase

Abstract The main objective of current study was to investigate the chemopreventive and chemotherapeutic activity of Artemisia vulgaris extract on diethylnitrosoamine induced hepatocarcinogenesis in Balb C mice. Diethylnitrosoamine (DEN: 0.9%) was prepared to induce hepatocarcinoma in Balb C mice. The extract Artemisia vulgaris (AV) was prepared by maceration technique. Mice were classified into four groups as follows: Group 1 a control group (N=7) received saline solution (3.5 μl/mg), group 2 (N=14) received diethylnitrosoamine (3.5 μl/mg) intraperitoneally once in a week for eight consecutive weeks, group 3 (N=7) received only plant extract (AV: 150 mg/kg (Body weight) once in a week, while group 4 (N=7) was given in combination of diethylnitrosoamine (3.5 μl/mg) and plant extract (AV: 150 mg/kg (body weight). After eight weeks of DEN administration, mice of group 2 were divided into two subgroups containing seven mice each; subgroup 1 was sacrificed while subgroup 2 was treated with plant extract only (150 mg/kg (body weight)) once in a week for eight consecutive weeks. The DEN injected mice significant decline in levels of albumin with concomitant significant elevations such as aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, alpha feto protein, gamma glutamyl transferase, 5 nucleotidase, glucose-6-phosphate dehydrogenase and bilirubin. The administration of A. vulgaris significantly decreased the DEN induced hepatotoxicity. Present study revealed the potential anti-cancerous nature of Artemisia vulgaris, both in case of chemopreventive and post-treatment of A. vulgaris. Further studies are needed to explore the mechanism of prevention and therapy.

scholarly journals Preliminary Study on the Effect of Methanolic Extract of Watermelon (Citrullus lanatus) Rind on Prednisolone Suppressed Immunity in Male Wistar Rats

2021 ◽  
pp. 1-6
Author(s):  
Nyejirime Young Wike ◽  
Mobisson Samuel Kelechi ◽  
Godspower Onyeso ◽  
Okekem Amadi ◽  
Elizabeth Eepho Krukru
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Methanolic Extract ◽  
Citrullus Lanatus ◽  
Control Group ◽  
Group 4 ◽  
Watermelon Rind ◽  
Male Wistar Rats ◽  
Group 3 ◽  
Group 2

Citrullus lanatus thumb (Cucurbitaceae) commonly called watermelon is widely consumed in this part of the world as food and medicine. This study was carried out to examine the effect of methanolic extract of watermelon (Citrullus lanatus) rind on prednisolone suppressed immunity in male wistar rats. A total of 20 male wistar rats weighing 150-294g were used in 4 groups with five rats each. Group 1, the control group was given distilled water and feed, Group 2 was given 200 mg/kg body weight of methanolic extract of watermelon rind, Group 3 rats were given 2.5 mg/kg body weight of prednisolone and Group 4 rats were given 2.5 mg/kg body weight of prednisolone and 200 mg/kg body weight of methanolic extract of watermelon rind. Prednisolone and the methanolic extract of watermelon rind were administered orally for a period of 30 days. Blood samples were collected by cardio puncture from the rats for white blood cell (WBC), lymphocyte, and granulocyte and monocyte counts at the end of the experiment.  The data were statistically analysed using one-way ANOVA (Analysis of variance). Data were considered significant at p<0.05. The results obtained showed that methanolic extract of watermelon rind caused a significant increasen in immune function of rats when compared with the control and immune suppressed rats.

scholarly journals PRODUCTIVE AND BIOLOGICAL TRAITS OF YOUNGS DIFFERENT GENOTYPES OF KAZAKH WHITE-HEADED BREED

2019 ◽  
Vol 4 (2) ◽  
pp. 94-101
Author(s):  
Асемгул Бактыгалиева ◽  
Asemgul Baktygalieva ◽  
Киниспай Джуламанов ◽  
Kinispai Dzhulamanov ◽  
Андрей Ухтверов ◽  
...  
Keyword(s):  
Body Weight ◽  
Positive Impact ◽  
Biological Traits ◽  
Group 4 ◽  
Group 3 ◽  
White Cattle ◽  
Group 5 ◽  
Group 2 ◽  
West Kazakhstan ◽  
Meat Productivity

The research aim is increasing the potential of meat productivity of young Shagatay Zavodska type of livestock based on a heterogeneous selection of Shagatay cows with bulls – Ural type of hereford. The experience carried out on the breeding farm «Sabit» in West Kazakhstan region according to the scheme: group 1 – bull, group 2 – castrated males, group 3 – heifers of Shagatay breed of Kazakh white cattle and hybrids from crossing Shagatay cows with bulls of Ural Hereford, group 4 – bulls, 5 group – castrated males, 6 group – heifers. Groups were formed on the principle of analogues of 12 animals. Bulls by the age of 15 months of the Ural hereford reached the highest body weight and surpassed shagatai peers taking into account this selection feature in regard to sex by 20.4 kg. In castrates at the age of 18 months, this difference was in favor of the youngsters of group 5 and was 23.0 kg. Heter-ogeneous selection had a positive impact on the important productive qualities by heifers. Youngsters of group 6, born due to servicing bulls of the Ural Hereford, dominated in weight in regard to all analyzed periods. The contents of lizo the meyerhold centre in both winter and summer was greater at animal – hybrids of the Ural hereford and Shagatay animals, winter amount was 0,45-0,94 µg/ml in the summer – from 0.06 to 1.97 µg/ml. Significantly lower bactericidal activity of blood serum and slightly increased amount of lysozyme was shown by hybrids from the Ural hereford in the winter. Regardless of gender, the greatest consumption of both protein (8-41 g) and energy (0.48-2.54 MJ) per unit of body weight gained was characterized by descendants from parents of the Shagatai type of Ka-zakh white-headed breed. Hybrids the offspring of the Ural hereford – used crude protein in feeding more often for the synthesis of dietary protein.

scholarly journals Centella Asiatica Extracts Regulates Aluminium Chloride-Induced Neurotoxicity in Rats: Impact on Inflammation, Apoptosis and Biogenic Amine Levels

2018 ◽  
Vol 2 (1) ◽  
pp. 1-8
Author(s):  
Shaik Amjad ◽  
Keyword(s):  
Body Weight ◽  
Therapeutic Potential ◽  
Centella Asiatica ◽  
Aluminium Chloride ◽  
Albino Rats ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Wistar Albino Rats ◽  
Group 1

investigate the therapeutic potential of CA against chronic Aluminium Chloride (AlCl3) exposure induced rats. Wistar albino rats were segregated into four groups: group 1-control rats, group 2-rats received AlCl3 (300 mg/kg body weight, every day orally) for 60 days, rats in group 3-received CA (500 mg/kg body weight, orally) and group 4 rats were initiated with both AlCl3 and CA treatment.

scholarly journals Effects of brown rice, red rice and purple rice on body weight and serum lipid in mice fed a high fat diet

2018 ◽  
Vol 1 (T5) ◽  
pp. 27-34 ◽  
Author(s):  
Van Ngoc Thanh Nguyen ◽  
Thu Le Anh Nguyen ◽  
Hong Ngoc Nguyen
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Brown Rice ◽  
Red Rice ◽  
High Fat ◽  
Lower Weight ◽  
Group 4 ◽  
Group 3 ◽  
Lower Weight Gain ◽  
Group 2

Brown rice, purple rice and red rice are the basic components of macrobiotic diet. The aim of study was to examine the lower weight gain and antihyperlipidemic activities of brown rice, purple rice and red rice on mice fed high fat diet. The mice were divided into 5 groups: group 1 was normal control mice fed with normal pellet diet; group 2- hyperlipidemic control mice was fed with high fat (15 % wt/wt)/cholesterol (0.5 % wt/wt) supplemented with either corn starch (50 % wt/wt) or cooked brown rice - group 3 or cooked red rice –group 4 or cooked purple rice–group 5 (50 % wt/wt) as the main carbohydrate source for 6 weeks. The results showed that group 2 exhibited significantly increased on weight body and total cholesterol (TC), triglyceride (TG), LDL-cholesterol (LDL-c) and decreased in HDL-cholesterol (HDL-c) as compared to control group 1. Group 3, 4, 5 exhibited reduction on body weight and significantly decreased in the elevated TC, LDL-c and TG as compared to group 2. Among them, the reduction in body weight and TC and TG of group 4 was lower than other groups; the increase of HDL-c of group 5 was higher than group 3 and group 4. This is the first report identifying lower weight gain and anti-hyperlipidemic activities of cooked brown rice, cooked purple rice and cooked red rice on mice fed high fat diet. The results obtained in the present study indicate that brown rice, red rice and purple rice are health food for lower weight gain, anti-hyperlipidemic agent and prevention of heart diseases.

scholarly journals Hematology of 2, 4 (Dinitrophenyl Hydrazine) Induced Anaemic Rat Administered with Ficus capensis Fruits and Leave Extract

2020 ◽  
pp. 41-49
Author(s):  
N. N. Umerah ◽  
J. I. Okoye ◽  
A. I. Asouzu
Keyword(s):  
Body Weight ◽  
Blood Cell ◽  
Blood Parameters ◽  
Haematological Parameters ◽  
Group 4 ◽  
Group I ◽  
Group 3 ◽  
The Mean ◽  
Group 2 ◽  
Group 1

Background: Anemia is regarded as public health challenge and is predominant in developing countries due to nutritional deficiencies. Aim/Objectives: The study was carried out to evaluate the biological properties of Ficus capensis fruits and vegetables on some haematological parameters in 2, 4 (dinitrophenyl hydrazine) induced anemic rat. Materials and Methods: Ficus capensis leaves and fruits were separately plucked, sorted cleaned. Twenty male adult rats were purchased from the Department of Veterinary Pathology, University of Nigeria, Nsukka. The animals were divided into 4 groups of 5 rats each on the basis of body weight. The rats in all the groups received 2, 4-DNPH (20 mg/kg body weight) once daily for 7 days to induce anaemia. Group I were fed rat chow alone, group 2 were fed rat chow with ferrous sulphate, group 3 were fed rat chow with Ficus capensis leaves extract and group 4 were fed rat chow with Ficus capensis seed extract. The aqueous extracts of the leaves and fruits were tested for haematinic effects in albino rats. Blood parameters such as Packed Cell Volume (PCV), Red Blood Cell (RBC) count, White Blood Cell (WBC) count and Haemoglobin concentration (Hb) were measured. Results: The result showed that the mean PCV baseline of the rats were (38.72-39.24%), mean PCV of anemic rats (33.01- 34.60%) and the mean PCV of the rats after test of recovery were group 1 (34.10%), group 2 (51.81%), group 3 (40.20%) and group 4 (38.20%).The result showed that the mean HB baseline of the rats were (9.67-10.47 g/dl), mean HB of anemic rats (6.50- 7.10 g/dl) and the mean HB of the rats after test of recovery were group 1 (6.51 g/dl), group 2 (12.32 g/dl), group 3 (9.73 g/dl) and group 4 (9.69 g/dl). The results of the effect of the extracts on the haematological parameters indicated that oral administration of the aqueous extract of Ficus capensis leaves and fruits after 22 days exhibited a significant (P < 0.05) increase in haematinic activity by increasing the blood parameters Hb, PCV, WBC and RBC.

scholarly journals Acetylsalicylic acid (Aspirin®) and liver regeneration: experimental study in rats

2021 ◽  
Vol 48 ◽  
Author(s):  
MARIA DE LOURDES PESSOLE BIONDO-SIMÕES ◽  
VICTOR CEZAR DE AZEVEDO PESSINI ◽  
CAROLINA AYUMI ICHI ◽  
ROGÉRIO RIBEIRO ROBES ◽  
SÉRGIO IOSHII
Keyword(s):  
Body Weight ◽  
Acetylsalicylic Acid ◽  
Partial Hepatectomy ◽  
Daily Administration ◽  
Group 4 ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Experimental Group ◽  
Group 1

ABSTRACT Objective: to evaluate the influence of acetylsalicylic acid (ASA) on cell proliferation after partial hepatectomy in rats. Methods: 40 male Wistar rats were separated into four groups of ten rats each. Groups 1 and 2 (controls): undergoing 30% partial hepatectomy and, after one day (group 1) and seven days (group 2), to euthanasia; daily administration of 0.9% saline solution (1mL per 200g of body weight). Groups 3 and 4 (experimental): undergoing 30% partial hepatectomy and, after one day (group 3) and seven days (group 4), to euthanasia; daily administration of ASA (40mg/mL, 1mL per 200g of body weight). The absolute number of cells stained with PCNA was counted in photomicrographs, in five fields, and it was calculated the mean of positive cells per animal and per group. Results: the final mean of PCNA+ cells per group was: in group 1, 17.57 ± 6.77; in group 2, 19.31 ± 5.30; in group 3, 27.46 ± 11.55; and, in group 4, 12.40 ± 5.23. There was no significant difference at the two evaluation times in the control group (p=0.491), but there was in the experimental group (p=0.020), with a lower number of PCNA+ cells on the seventh day. The comparison between the two groups, on the first day, showed more PCNA+ cells in the livers of the animals that received ASA (p=0.047), and on the seventh day the number was lower in the experimental group (p=0.007). Conclusion: ASA induced greater hepatocyte proliferation.

scholarly journals Effect of spreader size on microleakage of roots filled with cold lateral compaction technique

2015 ◽  
Vol 09 (03) ◽  
pp. 378-381
Author(s):  
Tugba Turk ◽  
Beyser Piskin ◽  
Hasan Orucoglu ◽  
Berdan Aydin
Keyword(s):  
Maxillary Incisor ◽  
Human Teeth ◽  
Fluid Filtration ◽  
Root Canals ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Apical Leakage ◽  
Group 1

ABSTRACT Objectives: To evaluate the effect of spreader size on apical leakage of maxillary incisor teeth. Materials and Methods: A total of 75 permanent human teeth with no carious and no fracture or crack were used for this study. After removing the crown from the cementoenamel junction and the standardization of the root lengths, the specimens were randomly divided into five groups: Group 1 - Roots were not instrumented. Group 2 - Root canals were enlarged using the step-back technique to a #40 file and filled using cold lateral compaction (CLC) of gutta-percha (GP). Group 3 - During the filling procedure, the first spreader used was size 40. Group 4 - The first spreader used was size 35. Group 5 - The initial spreader used was size 25. The amount of leakage through the filled root canals was evaluated by computerized fluid filtration model. Statistical analyzes were done using Kruskal–Wallis test and Mann–Whitney test (P < 0.05). Results: There were statistically significant differences among the groups (P < 0.05). While the uninstrumented group (Group 1) had no leakage, instrumented but not filled roots (Group 2) demonstrated the highest leakage values. There were no differences between Group 3 and 4. Group 5 showed significantly less leakage than Group 3 and 4. Conclusion: Spreader size used during CLC of GP appeared to be a significant factor on apical leakage of roots. Using smaller size spreader during CLC may provide relatively less leakage.

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