The association of erythrocyte aggregation index and acute phase proteins after cardiac surgery with CPB

Objectives. The changes in the serum levels of lipopolysaccharide binding protein (LBP) and sCD14 during cardiac surgery were followed in this study.Design. Thirty-four patients, 17 in each group, were randomly assigned to coronary artery bypass grafting surgery performed either with (“on-pump”) or without (“off-pump”) cardiopulmonary bypass. LBP and sCD14 were evaluated by ELISA.Results. The serum levels of LBP were gradually increased from the 1st postoperative day and reached their maximum on the 3rd postoperative day in both “on-pump” and “off-pump” patients (30.33±9.96μg/mL;37.99±16.58μg/mL), respectively. There were no significant differences between “on-pump” and “off-pump” patients regarding LBP. The significantly increased levels of sCD14 from the 1st up to the 7th postoperative day in both “on-pump” and “off-pump” patients were found with no significant differences between these groups. No correlations between LBP and sCD14 and IL-6, CRP and long pentraxin PTX3 levels were found.Conclusions. The levels of LBP and sCD14 are elevated in cardiac surgical patients being similar in both groups. These molecules are not produced as acute phase proteins in these patients.

Download Full-text

Contribution of Acute-Phase Proteins and Cardiovascular Risk Factors to Erythrocyte Aggregation in Normolipidemic and Hyperlipidemic Individuals

Thrombosis and Haemostasis ◽

10.1055/s-0037-1615386 ◽

1998 ◽

Vol 80 (12) ◽

pp. 903-908 ◽

Cited By ~ 37

Author(s):

Xiaoduan Weng ◽

Ghislaine Roederer ◽

Raymond Beaulieu ◽

Guy Cloutier

Keyword(s):

Risk Factors ◽

Cardiovascular Risk ◽

Cardiovascular Risk Factors ◽

Acute Phase ◽

Adhesive Strength ◽

Acute Phase Proteins ◽

Erythrocyte Aggregation ◽

Rbc Aggregation ◽

Rouleau Formation ◽

Kinetics Of

SummaryBackground. Numerous studies have demonstrated that elevated concentrations of acute-phase proteins affect red blood cell (RBC) aggregation. Plasma lipids and lipoproteins were also shown to be correlated with RBC aggregation in hypercholesterolemia. However, whether acute-phase proteins promote RBC hyperaggregation in hyper-lipidemic patients is unknown. The main objective of the study was to identify the impact of acute-phase proteins such as fibrinogen (Fib), haptoglobin (Hp), ceruloplasmin (Cp), α1-acid glycoprotein (AGP), α1-antitrypsin (AT), immunoglobulin G (IgG), and albumin (Alb) on RBC aggregation in 35 hyperlipidemic patients. The influence of these proteins in 32 normolipidemic subjects was also determined.Methods and Results. RBC aggregation parameters reflecting the kinetics of rouleau formation and the adhesive strength between RBCs were measured by laser reflectometry. Multivariate forward stepwise linear regression analyses were performed to study the relationship between RBC aggregation and these acute-phase proteins, total cholesterol (TC), triglycerides (TG), high (HDL-C) and low (LDL-C) density lipo-protein cholesterol, age, gender, body mass index (BMI), mean blood pressure (Mpressure), and smoking habit. The kinetics of rouleau formation was positively correlated with the linear combination of IgG and Hp (r = 0.76, p <0.0001) in hyperlipidemic patients, whereas IgG, smoking, AGP and gender were significant independent predictors in healthy subjects (r = 0.79, p <0.0001). The correlations obtained for the models predicting the adhesive strength between RBCs were 0.69 in patients (Alb, HDL-C, IgG, p <0.002) and 0.71 in healthy individuals (AGP, BMI, p <0.0001).Conclusion. This study suggests that acute-phase proteins such as IgG, Hp, AGP and Alb influence significantly and in an independent way the level of RBC aggregation. The close association between RBC aggregation and cardiovascular risk factors further strengthens its clinical importance.

Download Full-text

Increased erythrocyte aggregation in infectious diseases: Influence of “acute phase proteins”

Clinical Hemorheology and Microcirculation ◽

10.3233/ch-1984-42-314 ◽

2016 ◽

Vol 4 (2-3) ◽

pp. 237-244 ◽

Cited By ~ 1

Author(s):

E. Friederichs ◽

J. Germs ◽

M. Lakomek ◽

H. Winkler ◽

W. Tillmann

Keyword(s):

Infectious Diseases ◽

Acute Phase ◽

Acute Phase Proteins ◽

Erythrocyte Aggregation

Download Full-text

Influence of acute-phase proteins on erythrocyte aggregation

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1996.271.6.h2346 ◽

1996 ◽

Vol 271 (6) ◽

pp. H2346-H2352 ◽

Cited By ~ 18

Author(s):

X. Weng ◽

G. Cloutier ◽

R. Beaulieu ◽

G. O. Roederer

Keyword(s):

Acute Phase ◽

Acute Phase Proteins ◽

Shear Resistance ◽

Progressive Increase ◽

Erythrocyte Aggregation ◽

Aggregation Kinetics ◽

Reactive Protein ◽

Specific Receptors ◽

Alpha 1 Antitrypsin ◽

Adhesive Forces

With the exception of fibrinogen, immunoglobulins, and albumin, little information is available on the effect of acute-phase proteins on erythrocyte aggregation. The objective of this study was to investigate the effects of haptoglobin (Hp), C-reactive protein (CRP), ceruloplasmin (Cp), alpha 1-acid glycoprotein (alpha 1-AGP), and alpha 1-antitrypsin (alpha 1-AT) on the aggregation kinetics and shear resistance of erythrocyte aggregates. The plasma concentration of these proteins was measured in 20 healthy individuals and kept unchanged while the concentration of the protein tested was increased. Adding Hp to concentrations between 2.78 and 4.99 g/l resulted in a significant progressive increase in aggregation kinetics compared with controls. An elevation of the shear resistance of the aggregates was found for CRP at a concentration of 0.438 g/l. By an increase in the concentration of Cp from 4.40 to 9.39 g/l, the aggregation kinetics and the adhesive forces between erythrocytes were significantly increased: No effect on erythrocyte aggregation was observed for alpha 1-AGP, alpha 1-AT, and Cp at concentrations of 2.85, 3.97, and 2.43 g/l, respectively. The molecular mass of the acute-phase proteins, their configuration, and the presence of specific receptors on the erythrocyte membrane are postulated as possible factors influencing erythrocyte aggregation.

Download Full-text

Keratins 8/18 represent type II acute-phase proteins which are differentially regulated in human liver disease

Zeitschrift für Gastroenterologie ◽

10.1055/s-0032-1331972 ◽

2013 ◽

Vol 51 (01) ◽

Author(s):

N Güldiken ◽

V Usachov ◽

K Levada ◽

M Ziol ◽

P Nahon ◽

...

Keyword(s):

Liver Disease ◽

Acute Phase ◽

Human Liver ◽

Acute Phase Proteins ◽

Type Ii ◽

Human Liver Disease

Download Full-text

Supplementation of postbiotic RI11 improves antioxidant enzymes activity, upregulated gut barrier genes and reduced cytokines, acute phase proteins and heat shock protein70 gene expression levels in heat-stressed broilers

Poultry Science ◽

10.1016/j.psj.2020.12.011 ◽

2020 ◽

Author(s):

Ali Merzza Humam ◽

Teck Chwen Loh ◽

Hooi Ling Foo ◽

Wan Ibrahim Izuddin ◽

Idrus Zulkifli ◽

...

Keyword(s):

Gene Expression ◽

Antioxidant Enzymes ◽

Heat Shock ◽

Acute Phase ◽

Acute Phase Proteins ◽

Gut Barrier ◽

Expression Levels ◽

Enzymes Activity ◽

Gene Expression Levels

Download Full-text

Comparison of host endothelial, epithelial and inflammatory response in ICU patients with and without COVID-19: a prospective observational cohort study

Critical Care ◽

10.1186/s13054-021-03547-z ◽

2021 ◽

Vol 25 (1) ◽

Author(s):

Pavan K. Bhatraju ◽

Eric D. Morrell ◽

Leila Zelnick ◽

Neha A. Sathe ◽

Xin-Ya Chai ◽

...

Keyword(s):

Endothelial Dysfunction ◽

Epithelial Cell ◽

Acute Phase ◽

Cell Injury ◽

Acute Phase Proteins ◽

Invasive Mechanical Ventilation ◽

Icu Patients ◽

Icu Admission ◽

Amyloid A ◽

Epithelial Cell Injury

Abstract Background Analyses of blood biomarkers involved in the host response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral infection can reveal distinct biological pathways and inform development and testing of therapeutics for COVID-19. Our objective was to evaluate host endothelial, epithelial and inflammatory biomarkers in COVID-19. Methods We prospectively enrolled 171 ICU patients, including 78 (46%) patients positive and 93 (54%) negative for SARS-CoV-2 infection from April to September, 2020. We compared 22 plasma biomarkers in blood collected within 24 h and 3 days after ICU admission. Results In critically ill COVID-19 and non-COVID-19 patients, the most common ICU admission diagnoses were respiratory failure or pneumonia, followed by sepsis and other diagnoses. Similar proportions of patients in both groups received invasive mechanical ventilation at the time of study enrollment. COVID-19 and non-COVID-19 patients had similar rates of acute respiratory distress syndrome, severe acute kidney injury, and in-hospital mortality. While concentrations of interleukin 6 and 8 were not different between groups, markers of epithelial cell injury (soluble receptor for advanced glycation end products, sRAGE) and acute phase proteins (serum amyloid A, SAA) were significantly higher in COVID-19 compared to non-COVID-19, adjusting for demographics and APACHE III scores. In contrast, angiopoietin 2:1 (Ang-2:1 ratio) and soluble tumor necrosis factor receptor 1 (sTNFR-1), markers of endothelial dysfunction and inflammation, were significantly lower in COVID-19 (p < 0.002). Ang-2:1 ratio and SAA were associated with mortality only in non-COVID-19 patients. Conclusions These studies demonstrate that, unlike other well-studied causes of critical illness, endothelial dysfunction may not be characteristic of severe COVID-19 early after ICU admission. Pathways resulting in elaboration of acute phase proteins and inducing epithelial cell injury may be promising targets for therapeutics in COVID-19.

Download Full-text