scholarly journals Curcumin-loaded graphene oxide flakes as an effective antibacterial system against methicillin-resistant Staphylococcus aureus

2018 ◽  
Vol 8 (3) ◽  
pp. 20170059 ◽  
Author(s):  
F. Bugli ◽  
M. Cacaci ◽  
V. Palmieri ◽  
R. Di Santo ◽  
R. Torelli ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Graphene Oxide ◽  
Drug Loading ◽  
Water Environment ◽  
Antibacterial Properties ◽  
Public Health Problem ◽  
Major Constituent ◽  
Global Public Health ◽  
Methicillin Resistant ◽  
Bacterial Membranes

Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for serious hospital infections worldwide and represents a global public health problem. Curcumin, the major constituent of turmeric, is effective against MRSA but only at cytotoxic concentrations or in combination with antibiotics. The major issue in curcumin-based therapies is the poor solubility of this hydrophobic compound and the cytotoxicity at high doses. In this paper, we describe the efficacy of a composite nanoparticle made of curcumin (CU) and graphene oxide (GO), hereafter GOCU, in MRSA infection treatment. GO is a nanomaterial with a large surface area and high drug-loading capacity. GO has also antibacterial properties due mainly to a mechanical cutting of the bacterial membranes. For this physical mechanism of action, microorganisms are unlikely to develop resistance against this nanomaterial. In this work, we report the capacity of GO to support and stabilize curcumin molecules in a water environment and we demonstrate the efficacy of GOCU against MRSA at a concentration below 2 µg ml −1 . Further, GOCU displays low toxicity on fibroblasts cells and avoids haemolysis of red blood cells. Our results indicate that GOCU is a promising nanomaterial against antibiotic-resistant MRSA.

Graphene Oxide Nanosheets with Efficient Antibacterial Activity Against Methicillin-Resistant Staphylococcus aureus (MRSA)

2021 ◽  
Vol 17 (8) ◽  
pp. 1627-1634
Author(s):  
Yujie Gao ◽  
Yuanhao Dong ◽  
Yubin Cao ◽  
Wenlong Huang ◽  
Chenhao Yu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Graphene Oxide ◽  
Public Health Problem ◽  
Resistant Bacteria ◽  
Methicillin Resistant ◽  
Antibacterial Performance ◽  
Drug Resistant Bacteria ◽  
Low Concentrations ◽  
Life Threatening

The development of drug-resistant bacteria has become a public health problem, among which methicillin-resistant Staphylococcus aureus (MRSA) leads to various life-threatening diseases. Graphene oxide (GO) is a two-dimensional nanomaterial with potential in the anti-MRSA treatment. This study prepared GO nanosheets with fixed lamellar size, investigated its antibacterial activity against MRSA, and analyzed the related antibacterial mechanisms. We found that the fabrication of GO with stable dispersion was workable. Furthermore, such GO had superior antibacterial performance against MRSA at low concentrations with the dose-dependent anti-MRSA effect. The GO-MRSA interaction also provided fundamental support for the antibacterial mechanisms with cleavage and encapsulation effects. In conclusion, GO nanosheets may be a promising antimicrobial agent against MRSA.

scholarly journals A selective membrane-targeting repurposed antibiotic with activity against persistent methicillin-resistant Staphylococcus aureus

2019 ◽  
Vol 116 (33) ◽  
pp. 16529-16534 ◽  
Author(s):  
Wooseong Kim ◽  
Guijin Zou ◽  
Taylor P. A. Hari ◽  
Ingrid K. Wilt ◽  
Wenpeng Zhu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Membrane Fluidity ◽  
Lipid Bilayers ◽  
Therapeutic Agent ◽  
Md Simulations ◽  
Persister Cells ◽  
Methicillin Resistant ◽  
Bacterial Membranes ◽  
Selective Membrane ◽  
Mrsa Infection

Treatment of Staphylococcus aureus infections is complicated by the development of antibiotic tolerance, a consequence of the ability of S. aureus to enter into a nongrowing, dormant state in which the organisms are referred to as persisters. We report that the clinically approved anthelmintic agent bithionol kills methicillin-resistant S. aureus (MRSA) persister cells, which correlates with its ability to disrupt the integrity of Gram-positive bacterial membranes. Critically, bithionol exhibits significant selectivity for bacterial compared with mammalian cell membranes. All-atom molecular dynamics (MD) simulations demonstrate that the selectivity of bithionol for bacterial membranes correlates with its ability to penetrate and embed in bacterial-mimic lipid bilayers, but not in cholesterol-rich mammalian-mimic lipid bilayers. In addition to causing rapid membrane permeabilization, the insertion of bithionol increases membrane fluidity. By using bithionol and nTZDpa (another membrane-active antimicrobial agent), as well as analogs of these compounds, we show that the activity of membrane-active compounds against MRSA persisters positively correlates with their ability to increase membrane fluidity, thereby establishing an accurate biophysical indicator for estimating antipersister potency. Finally, we demonstrate that, in combination with gentamicin, bithionol effectively reduces bacterial burdens in a mouse model of chronic deep-seated MRSA infection. This work highlights the potential repurposing of bithionol as an antipersister therapeutic agent.

scholarly journals Carriage Methicillin-Resistant Staphylococcus aureus in Poultry and Cattle in Northern Algeria

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Saliha Bounar-Kechih ◽  
Mossadak Taha Hamdi ◽  
Hebib Aggad ◽  
Nacima Meguenni ◽  
Zafer Cantekin
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Broiler Chickens ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Laying Hens ◽  
Methicillin Resistance ◽  
Public Health Problem ◽  
Cross Resistance ◽  
Isolation And Identification ◽  
Methicillin Resistant

Multiresistant and especially Methicillin-Resistant Staphylococcus aureus (MRSA) poses a serious public health problem that requires their immediate identification and antibiotic resistance characteristics. In order to determine antibiotic resistance S. aureus poultry and bovine origin, 8840 samples were collected from slaughterhouses in the northern region of Algeria between years 2009 and 2014. 8375 samples were from an avian origin (1875 from laying hens and 6500 from broiler chickens) and the rest was from bovine origin. Bacteriological isolation and identification were made by classical culture method and antibiotic resistance patterns were determined by disc diffusion test. The prevalence of S. aureus was 42% in laying hens, 12% in broilers, and 55% in bovine samples. The prevalence of MRSA was 57%, 50%, and 31% in laying hens, broiler chickens, and bovine, respectively. While MRSA strains isolated from poultry showed cross-resistance to aminoglycosides, fluoroquinolones, macrolides, sulphonamides, and cyclins, those isolated from bovine also revealed similar multiresistance except for sulphonamide. This high percentage of methicillin resistance and multidrug resistance in S. aureus poultry and bovine origin may have importance for human health and curing of human infections.

scholarly journals Copper Complexation Screen Reveals Compounds with Potent Antibiotic Properties against Methicillin-Resistant Staphylococcus aureus

2014 ◽  
Vol 58 (7) ◽  
pp. 3727-3736 ◽  
Author(s):  
Mehri Haeili ◽  
Casey Moore ◽  
Christopher J. C. Davis ◽  
James B. Cochran ◽  
Santosh Shah ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
High Throughput ◽  
Copper Ions ◽  
Antibacterial Properties ◽  
Rare Event ◽  
Innate Immune ◽  
Release Mechanism ◽  
Immune Functions ◽  
Methicillin Resistant ◽  
Copper Complexation

ABSTRACTMacrophages take advantage of the antibacterial properties of copper ions in the killing of bacterial intruders. However, despite the importance of copper for innate immune functions, coordinated efforts to exploit copper ions for therapeutic interventions against bacterial infections are not yet in place. Here we report a novel high-throughput screening platform specifically developed for the discovery and characterization of compounds with copper-dependent antibacterial properties toward methicillin-resistantStaphylococcus aureus(MRSA). We detail how one of the identified compounds, glyoxal-bis(N4-methylthiosemicarbazone) (GTSM), exerts its potent strictly copper-dependent antibacterial properties on MRSA. Our data indicate that the activity of the GTSM-copper complex goes beyond the general antibacterial effects of accumulated copper ions and suggest that, in contrast to prevailing opinion, copper complexes can indeed exhibit species- and target-specific activities. Based on experimental evidence, we propose that copper ions impose structural changes upon binding to the otherwise inactive GTSM ligand and transfer antibacterial properties to the chelate. In turn, GTSM determines target specificity and utilizes a redox-sensitive release mechanism through which copper ions are deployed at or in close proximity to a putative target. According to our proof-of-concept screen, copper activation is not a rare event and even extends to already established drugs. Thus, copper-activated compounds could define a novel class of anti-MRSA agents that amplify copper-dependent innate immune functions of the host. To this end, we provide a blueprint for a high-throughput drug screening campaign which considers the antibacterial properties of copper ions at the host-pathogen interface.

scholarly journals Antibacterial effects of wood structural components and extractives from Pinus sylvestris and Picea abies on methicillin-resistant Staphylococcus aureus and Escherichia coli O157:H7

BioResources ◽  
2017 ◽  
Vol 12 (4) ◽  
pp. 7601-7614 ◽  
Author(s):  
Tiina Vainio-Kaila ◽  
Xue Zhang ◽  
Tuomas Hänninen ◽  
Aino Kyyhkynen ◽  
Leena-Sisko Johansson ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Photoelectron Spectroscopy ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antibacterial Properties ◽  
Gas Chromatography Mass Spectrometry ◽  
Escherichia Coli O157 ◽  
Structural Components ◽  
Methicillin Resistant ◽  
Viable Bacteria

Antibacterial properties of wood structural components and extractives were investigated against methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli O157:H7 by placing bacterial inoculum on the model surfaces and incubating them for 2, 4, and 24 h. After incubation, the amount of viable bacteria on the surfaces was studied. The film coverage and thickness were evaluated with atomic-force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS). The extracts were analyzed with gas chromatography–mass spectrometry (GC-MS). The results showed that films fully covered the glass surfaces. The XPS results confirmed the analysis of GC-MS, which revealed more similarities between the extractives of pine heartwood and spruce heartwood than between pine heartwood and pine sapwood. Only the pine heartwood extract showed an antibacterial effect against E. coli O157:H7. In contrast, MRSA was susceptible to all of the extracts and milled wood lignin (MWL).

scholarly journals The first isolation of Panton-Valentine leukocidin (PVL) positive community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) in Russia

2007 ◽  
Vol 12 (11) ◽  
Author(s):  
T Baranovich ◽  
V Potapov ◽  
T Yamamoto
Keyword(s):  
Public Health ◽  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Public Health Problem ◽  
The United States ◽  
Important Public Health Problem ◽  
Methicillin Resistant ◽  
Important Public Health ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

Since the first report of pediatric deaths in 1997-1999 in the United States, community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) has become an increasingly important public health problem worldwide.

scholarly journals Characterization of Host and Bacterial Contributions to Lung Barrier Dysfunction Following Co-infection with 2009 Pandemic Influenza and Methicillin Resistant Staphylococcus aureus

Viruses ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 116 ◽  
Author(s):  
Michaela Nickol ◽  
Justine Ciric ◽  
Shane Falcinelli ◽  
Daniel Chertow ◽  
Jason Kindrachuk
Keyword(s):  
Public Health ◽  
Staphylococcus Aureus ◽  
Pandemic Influenza ◽  
Bacterial Infections ◽  
Influenza Pandemic ◽  
Public Health Problem ◽  
Influenza Viruses ◽  
Alveolar Epithelial Cells ◽  
Methicillin Resistant ◽  
Barrier Integrity

Influenza viruses are a threat to global public health resulting in ~500,000 deaths each year. Despite an intensive vaccination program, influenza infections remain a recurrent, yet unsolved public health problem. Secondary bacterial infections frequently complicate influenza infections during seasonal outbreaks and pandemics, resulting in increased morbidity and mortality. Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA), is frequently associated with these co-infections, including the 2009 influenza pandemic. Damage to alveolar epithelium is a major contributor to severe influenza-bacterial co-infections and can result in gas exchange abnormalities, fluid leakage, and respiratory insufficiency. These deleterious manifestations likely involve both pathogen- and host-mediated mechanisms. However, there is a paucity of information regarding the mechanisms (pathogen- and/or host-mediated) underlying influenza-bacterial co-infection pathogenesis. To address this, we characterized the contributions of viral-, bacterial-, and host-mediated factors to the altered structure and function of alveolar epithelial cells during co-infection with a focus on the 2009 pandemic influenza (pdm2009) and MRSA. Here, we characterized pdm2009 and MRSA replication kinetics, temporal host kinome responses, modulation of MRSA virulence factors, and disruption of alveolar barrier integrity in response to pdm2009-MRSA co-infection. Our results suggest that alveolar barrier disruption during co-infection is mediated primarily through host response dysregulation, resulting in loss of alveolar barrier integrity.

Preparation and antibacterial behaviour of nanostructured Ag@SiO2–penicillin with silver nanoplates

2019 ◽  
Vol 43 (42) ◽  
pp. 16612-16620 ◽  
Author(s):  
Mahdieh Malekzadeh ◽  
King Lun Yeung ◽  
Mohammad Halali ◽  
Qing Chang
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Properties ◽  
Methicillin Resistant ◽  
Silver Nanoplates

Preparation of Ag@SiO2–penicillin NPs with superior synergistic and antibacterial properties against methicillin-resistant Staphylococcus aureus.

scholarly journals First Complete Genome Sequence of Methicillin-Resistant Staphylococcus aureus Strain SO-1977 Isolated from Khartoum, Sudan

2017 ◽  
Vol 5 (42) ◽  
Author(s):  
Sofia B. Mohamed ◽  
Mohamed S. Ali ◽  
Faisal M. Alamir ◽  
Tahani B. Alyas ◽  
Abdallah E. Ahmed ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Public Health Problem ◽  
Aureus Strain ◽  
Methicillin Resistant ◽  
Content Type ◽  
Contaminated Wound

ABSTRACT Methicillin-resistant Staphylococcus aureus is increasingly becoming resistant to most antibiotics and consequently has become a challenging public health problem in Sudan. The present study documented the first complete genome sequence of strain SO-1977, isolated from a contaminated wound in Sudan.

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