scholarly journals Homogenization of cortical bone reveals that the organization and shape of pores marginally affect elasticity

2019 ◽  
Vol 16 (151) ◽  
pp. 20180911 ◽  
Author(s):  
Xiran Cai ◽  
Renald Brenner ◽  
Laura Peralta ◽  
Cécile Olivier ◽  
Pierre-Jean Gouttenoire ◽  
...  
Keyword(s):  
Cortical Bone ◽  
Volume Fraction ◽  
Transversely Isotropic ◽  
Pore Network ◽  
Anisotropic Elasticity ◽  
Homogenization Method ◽  
Femoral Diaphysis ◽  
Micro Computed Tomography ◽  
Architectural Features ◽  
Resonant Ultrasound

With ageing and various diseases, the vascular pore volume fraction (porosity) in cortical bone increases, and the morphology of the pore network is altered. Cortical bone elasticity is known to decrease with increasing porosity, but the effect of the microstructure is largely unknown, while it has been thoroughly studied for trabecular bone. Also, popular micromechanical models have disregarded several micro-architectural features, idealizing pores as cylinders aligned with the axis of the diaphysis. The aim of this paper is to quantify the relative effects on cortical bone anisotropic elasticity of porosity and other descriptors of the pore network micro-architecture associated with pore number, size and shape. The five stiffness constants of bone assumed to be a transversely isotropic material were measured with resonant ultrasound spectroscopy in 55 specimens from the femoral diaphysis of 29 donors. The pore network, imaged with synchrotron radiation X-ray micro-computed tomography, was used to derive the pore descriptors and to build a homogenization model using the fast Fourier transform (FFT) method. The model was calibrated using experimental elasticity. A detailed analysis of the computed effective elasticity revealed in particular that porosity explains most of the variations of the five stiffness constants and that the effects of other micro-architectural features are small compared to usual experimental errors. We also have evidence that modelling the pore network as an ensemble of cylinders yields biased elasticity values compared to predictions based on the real micro-architecture. The FFT homogenization method is shown to be particularly efficient to model cortical bone.

scholarly journals Combination of µCT and light microscopy for generation-specific stereological analysis of pulmonary arterial branches: a proof-of-concept study

2020 ◽  
Author(s):  
Roman Grothausmann ◽  
Jonas Labode ◽  
Pablo Hernandez-Cerdan ◽  
David Haberthür ◽  
Ruslan Hlushchuk ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Volume Fraction ◽  
Pulmonary Vasculature ◽  
Chronic Obstructive ◽  
Micro Computed Tomography ◽  
Vascular Perfusion ◽  
Arterial Tree ◽  
Color Code ◽  
Rabbit Lung ◽  
Stereological Analysis

AbstractVarious lung diseases, including pulmonary hypertension, chronic obstructive pulmonary disease or bronchopulmonary dysplasia, are associated with structural and architectural alterations of the pulmonary vasculature. The light microscopic (LM) analysis of the blood vessels is limited by the fact that it is impossible to identify which generation of the arterial tree an arterial profile within a LM microscopic section belongs to. Therefore, we established a workflow that allows for the generation-specific quantitative (stereological) analysis of pulmonary blood vessels. A whole left rabbit lung was fixed by vascular perfusion, embedded in glycol methacrylate and imaged by micro-computed tomography (µCT). The lung was then exhaustively sectioned and 20 consecutive sections were collected every 100 µm to obtain a systematic uniform random sample of the whole lung. The digital processing involved segmentation of the arterial tree, generation analysis, registration of LM sections with the µCT data as well as registration of the segmentation and the LM images. The present study demonstrates that it is feasible to identify arterial profiles according to their generation based on a generation-specific color code. Stereological analysis for the first three arterial generations of the monopodial branching of the vasculature included volume fraction, total volume, lumen-to-wall ratio and wall thickness for each arterial generation. In conclusion, the correlative image analysis of µCT and LM-based datasets is an innovative method to assess the pulmonary vasculature quantitatively.

scholarly journals Effect of Age on Bone Structure Parameters in Laying Hens

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 570
Author(s):  
Masayoshi Yamada ◽  
Chongxiao Chen ◽  
Toshie Sugiyama ◽  
Woo Kyun Kim
Keyword(s):  
Cortical Bone ◽  
Bone Quality ◽  
Bone Mineral ◽  
Volume Fraction ◽  
Bone Structure ◽  
Bone Volume ◽  
Egg Laying ◽  
Medullary Bone ◽  
Mineral Density ◽  
Tibial Diaphysis

Changes in medullary and cortical bone structure with age remain unclear. Twenty Hy-Line W36 hens, 25 or 52 weeks of age, were euthanized, and both tibiae were collected when an egg was present in the magnum. Serial cross sections of the tibiae were stained with Alcian blue. The bones were scanned using micro-computed tomography. Trabecular width (Tb.Wi) was significantly higher (p < 0.05) in 25-week-old hens, whereas medullary bone tissue volume (TV) was significantly higher (p < 0.01) in 52-week-old hens. 25-week-old hens had significantly higher (p < 0.01) bone volume fraction (BVF = calcified tissue / TV). Moreover, the cortical bone parameters were significantly higher (TV and bone mineral content (BMC) at p < 0.05, and bone volume (BV) and BVF at p < 0.01) in younger hens. Open porosity and total porosity, which indicate less density, were significantly higher (p < 0.01) in older hens. Older hens showed significantly higher (p < 0.01) tibial diaphysis TV than younger hens. Younger hens had significantly higher (p < 0.01) BV, BVF and bone mineral density (BMD) of the tibial diaphysis. These findings reveal that reductions in medullary bone quality might be associated with age-related low estrogen levels and stimulation of osteoclastic bone resorption by parathyroid hormone. Cortical bone quality decreased with enlargement of the Haversian canals and loss of volume, with a longer egg-laying period leading to osteoporosis.

scholarly journals Inhibition of Bone Loss byCissus quadrangularisin Mice: A Preliminary Report

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Jameela Banu ◽  
Erika Varela ◽  
Ali N. Bahadur ◽  
Raheela Soomro ◽  
Nishu Kazi ◽  
...  
Keyword(s):  
Side Effects ◽  
Bone Loss ◽  
Cortical Bone ◽  
Proximal Tibia ◽  
Femoral Diaphysis ◽  
Minor Trauma ◽  
Alternative Medicines ◽  
Femoral Metaphysis ◽  
Cissus Quadrangularis ◽  
Distal Femoral Metaphysis

Women drastically loose bone during and after menopause leading to osteoporosis, a disease characterized by low bone mass increasing the risk of fractures with minor trauma. Existing therapies mainly reduce bone resorption, however, all existing drugs have severe side effects. Recently, the focus is to identify alternative medicines that can prevent and treat osteoporosis with minimal or no side effects. We usedCissus quadrangularis(CQ), a medicinal herb, to determine its effects on bone loss after ovariectomy in C57BL/6 mice. Two-month old mice were either sham operated or ovariectomized and fed CQ diet. After eleven weeks, mice were sacrificed and the long bones scanned using pQCT andμCT. In the distal femoral metaphysis, femoral diaphysis, and proximal tibia, control mice had decreased cancellous and cortical bone, while CQ-fed mice showed no significant differences in the trabecular number, thickness, and connectivity density, between Sham and OVX mice, except for cortical bone mineral content in the proximal tibia. There were no changes in the bone at the tibio-fibular junction between groups. We conclude that CQ effectively inhibited bone loss in the cancellous and cortical bones of femur and proximal tibia in these mice.

scholarly journals Trabecular bone patterning in the hominoid distal femur

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5156 ◽  
Author(s):  
Leoni Georgiou ◽  
Tracy L. Kivell ◽  
Dieter H. Pahr ◽  
Matthew M. Skinner
Keyword(s):  
Trabecular Bone ◽  
Distal Femur ◽  
Volume Fraction ◽  
Reaction Force ◽  
Intraspecific Variability ◽  
Trabecular Structure ◽  
Trabecular Architecture ◽  
Micro Computed Tomography ◽  
Bone Volume Fraction ◽  
Distal Epiphysis

BackgroundIn addition to external bone shape and cortical bone thickness and distribution, the distribution and orientation of internal trabecular bone across individuals and species has yielded important functional information on how bone adapts in response to load. In particular, trabecular bone analysis has played a key role in studies of human and nonhuman primate locomotion and has shown that species with different locomotor repertoires display distinct trabecular architecture in various regions of the skeleton. In this study, we analyse trabecular structure throughout the distal femur of extant hominoids and test for differences due to locomotor loading regime.MethodsMicro-computed tomography scans ofHomo sapiens(n= 11),Pan troglodytes(n= 18),Gorilla gorilla(n= 14) andPongosp. (n= 7) were used to investigate trabecular structure throughout the distal epiphysis of the femur. We predicted that bone volume fraction (BV/TV) in the medial and lateral condyles inHomowould be distally concentrated and more anisotropic due to a habitual extended knee posture at the point of peak ground reaction force during bipedal locomotion, whereas great apes would show more posteriorly concentrated BV/TV and greater isotropy due to a flexed knee posture and more variable hindlimb use during locomotion.ResultsResults indicate some significant differences between taxa, with the most prominent being higher BV/TV in the posterosuperior region of the condyles inPanand higher BV/TV and anisotropy in the posteroinferior region inHomo. Furthermore, trabecular number, spacing and thickness differ significantly, mainly separatingGorillafrom the other apes.DiscussionThe trabecular architecture of the distal femur holds a functional signal linked to habitual behaviour; however, there was more similarity across taxa and greater intraspecific variability than expected. Specifically, there was a large degree of overlap in trabecular structure across the sample, andHomowas not as distinct as predicted. Nonetheless, this study offers a comparative sample of trabecular structure in the hominoid distal femur and can contribute to future studies of locomotion in extinct taxa.

Quantitative micro-computed tomography analysis and rheological investigation of Nitrile rubber/Rockwool composites

2021 ◽  
pp. 002199832110338
Author(s):  
Elisson BD da Rocha ◽  
Ana Maria F de Sousa ◽  
Ana Lúcia N da Silva ◽  
Cristina RG Furtado ◽  
Marcos V Colaço ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Elastic Modulus ◽  
Aspect Ratio ◽  
Volume Fraction ◽  
Nitrile Rubber ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Rubber Composites ◽  
Ratio Data ◽  
Ct Analysis

This study reports the reinforcement degree investigation of two types of rockwool fibers (F1 and F2), in nitrile rubber composites. The micro-computed tomography (micro-CT) 3D images showed that both fibers were well-dispersed in the NBR matrix, without a preferential orientation. The micro-CT analysis also allowed quantifying volume fraction, inter-fiber distance, and aspect ratio. Those morphometric parameters were used for supporting the composites rheological behavior assessment. Changes in the elastic modulus and phase angle followed the same trend of the inter-fiber distance values, regardless the type of fiber. Both volume fraction and aspect ratio data from the micro-CT analysis were used to predict theoretical values of elastic modulus using the Guth-Gold and modified Guth-Gold equations, and the results obtained were compared to the rheological experimental data. This analysis was helpful to better understand the rockwool fibers reinforcement degree differences in the production of the nitrile rubber composites.

Micro-computed tomography analysis of natural fiber and bio-matrix tubular-braided composites

2019 ◽  
Vol 53 (28-30) ◽  
pp. 4003-4013 ◽  
Author(s):  
Brianna M Bruni-Bossio ◽  
Garrett W Melenka ◽  
Cagri Ayranci ◽  
Jason P Carey
Keyword(s):  
Computed Tomography ◽  
Natural Fiber ◽  
Volume Fraction ◽  
Void Content ◽  
Micro Computed Tomography ◽  
Braided Composites ◽  
Fiber Volume ◽  
Increasing Demand ◽  
Materials Used ◽  
Curing Method

There is an increasing demand for the use of “green”-based materials as reinforcement and matrix materials in composites. However, the ability of these natural-based materials to perform as consistently and reliably as conventional materials is still relatively unknown. A key importance in the viability of these materials is the evaluation of the content of voids and imperfections, which may affect the properties of the entire composite. In this study, the microstructure of tubular-braided composites manufactured from cellulose fibers and a partially bio-derived resin was studied with the use of micro-computed tomography. These methods were used to determine the effect of modifying braid angle, resin type, and curing method on fiber volume fraction, void volume, and void distribution. It was determined that the void content increased with the increase in braid angle, and vacuum-bagging reduced the total void content. The sample with the smallest braid angle produced with vacuum-bagged curing contained a void fraction of 1.5%. The results of this study proved that the materials used could be viable for further testing and development and that micro-computed tomography imaging is valuable for identifying how to improve consistency and minimize imperfections to create more accurate and reliable natural fiber-braided composites.

scholarly journals Anisotropic elastic properties of human cortical bone tissue inferred from inverse homogenization and resonant ultrasound spectroscopy

Materialia ◽  
2020 ◽  
Vol 11 ◽  
pp. 100730 ◽  
Author(s):  
Xiran Cai ◽  
Laura Peralta ◽  
Renald Brenner ◽  
Gianluca Iori ◽  
Didier Cassereau ◽  
...  
Keyword(s):  
Cortical Bone ◽  
Bone Tissue ◽  
Elastic Properties ◽  
Resonant Ultrasound Spectroscopy ◽  
Inverse Homogenization ◽  
Human Cortical Bone ◽  
Resonant Ultrasound ◽  
Anisotropic Elastic

Saint-Venant’s Principle and End Effects in Anisotropic Elasticity

1977 ◽  
Vol 44 (3) ◽  
pp. 424-430 ◽  
Author(s):  
I. Choi ◽  
C. O. Horgan
Keyword(s):  
Isotropic Material ◽  
Epoxy Composite ◽  
Transversely Isotropic ◽  
Anisotropic Elasticity ◽  
Decay Length ◽  
End Effects ◽  
Slow Decay ◽  
Rectangular Strip ◽  
Elasticity Problems ◽  
Transversely Isotropic Materials

The purpose of this paper is to draw attention to the fact that the routine application of Saint-Venant’s principle in the solution of elasticity problems involving highly anisotropic or composite materials is not justified in general. This is illustrated in the context of the plane problem of elasticity for an anisotropic rectangular strip loaded only on the short ends. For highly anisotropic transversely isotropic materials, the slow decay of end effects is demonstrated using a method involving self-equilibrating eigenfunctions. For a graphite/epoxy composite, for example, the characteristic decay length is shown to be approximately four times that for an isotropic material. The results have implications in the accurate measurement of mechanical properties of anisotropic materials.

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