The excystation of
colpoda
cucullus from its resting cysts, and the nature and properties of the cyst membranes
Having had occasion to make use of the resting cysts of Colpoda cucullus in the course of my work on the protozoa of the soil I was interested by the way in which the organisms escape from the confines of the cyst membranes. The processes involved were by no means obvious on somewhat casual observation, and it became necessary to study them in considerable detail before they could be fully elucidated. Moreover it was thought by working out as fully as possible the conditions involved in excystation some light might be thrown on the activity or inactivity of the protozoa in the soil. The water-content, available food supply, and temperature of any soil are obvious external factors in determining the possibility of protozoal activity, but that these were all the determining factors was by no means clear. There was the possibility that certain peculiar external influences were required for excystation, and if these could be determined it was possible that one would be able to say whether they were present or absent in a soil normally containing protozoa.