scholarly journals Rice NIN-LIKE PROTEIN 4 is a master regulator of nitrogen use efficiency

2020 ◽  
Author(s):  
Jie Wu ◽  
Zi-Sheng Zhang ◽  
Jing-Qiu Xia ◽  
Alamin Alfatih ◽  
Ying Song ◽  
...  
Keyword(s):  
N Uptake ◽  
Crop Improvement ◽  
Field Trials ◽  
Growth And Yield ◽  
Wild Type ◽  
Inorganic N ◽  
Master Regulator ◽  
Nitrogen Use ◽  
Use Efficiency ◽  
N Use

AbstractNitrogen (N) is one of the key essential macronutrients that affects rice growth and yield. Inorganic N fertilizers are excessively used to boost yield and generate serious collateral environmental pollution. Therefore, improving crop N use efficiency (NUE) is highly desirable and has been a major endeavor in crop improvement. However, only a few regulators have been identified that can be used to improve NUE in rice to date. Here we show that the NIN-like protein OsNLP4 significantly improves the rice NUE and yield. Field trials consistently showed that loss-of-OsNLP4 dramatically reduced yield and NUE compared with wild type under different N regimes. In contrast, the OsNLP4 overexpression lines remarkably increased yield by 30% and NUE by 47% under moderate N level compared with wild type. Transcriptomic analyses revealed that OsNLP4 orchestrates the expression of a majority of known N uptake, assimilation and signaling genes by directly binding to the nitrate-responsive cis-element in their promoters to regulate their expression. Moreover, overexpression of OsNLP4 can recover the phenotype of Arabidopsis nlp7 mutant and enhance its biomass. Our results demonstrate that OsNLP4 is a master regulator of NUE in rice and sheds light on crop NUE improvement.

scholarly journals Integrated use of farm manure and synthetic nitrogen fertilizer improves nitrogen use efficiency, yield and grain quality in wheat

2020 ◽  
Vol 15 (1) ◽  
pp. 29-34 ◽  
Author(s):  
Abdur Rehim ◽  
Maryam Khan ◽  
Muhammad Imran ◽  
Muhammad Amjad Bashir ◽  
Sami Ul-Allah ◽  
...  
Keyword(s):  
Nitrogen Use Efficiency ◽  
Block Design ◽  
N Uptake ◽  
N Fertilizer ◽  
N Use Efficiency ◽  
Growth And Yield ◽  
Limiting Factor ◽  
Nitrogen Use ◽  
Use Efficiency ◽  
N Use

Lower nitrogen use efficiency (NUE) is a major yield limiting factor in semi-arid regions due to poor organic contents of the soils. There is a close relationship between soil organic matter and NUE of fertilizers. Therefore, this study was conducted to assess the effect of sole N fertilizer and its combinations with organic amendments (farm manure combinations) on N use efficiency and crop productivity. For this purpose, a two-year field study was conducted to access the influence of integrated use of synthetic N fertilizer (urea) and farm manure on N use efficiency and wheat productivity. Treatments include i.e. Control, 100% N by Urea + 0%N by farm manure (FM), 75% N by Urea + 25 % N by FM, 50% N by Urea + 50% N by FM, 25% N by Urea + 75% N by FM, 0% N by Urea + 100% N by FM arranged in a triplicate randomized complete block design having recommended N rate of 150 kg ha–1. The results revealed that the treatment having 75% Urea and 25% FM followed by 50% Urea and 50% FM showed better results in term of wheat growth and yield. There was 98% increase in N uptake of wheat grains and 200% increase in NUE by the application of 75% urea+25% FM relative to sole application of urea. This study suggests use of 3:1 ratio of urea and FM for maximum NUE and sustainable wheat production.

scholarly journals Improvement of Root Characteristics Due to Nitrogen, Phosphorus, and Potassium Interactions Increases Rice (Oryza sativa L.) Yield and Nitrogen Use Efficiency

Agronomy ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 23
Author(s):  
Ming Du ◽  
Wenzhong Zhang ◽  
Jiping Gao ◽  
Meiqiu Liu ◽  
Yan Zhou ◽  
...  
Keyword(s):  
Grain Yield ◽  
Root Length ◽  
N Uptake ◽  
N Use Efficiency ◽  
Root Characteristics ◽  
Nitrogen Use ◽  
Total N ◽  
Absorption Area ◽  
Use Efficiency ◽  
N Use

Although nitrogen (N), phosphorus (P), and potassium (K) co-application improves crop growth, yield, and N use efficiency (NUE) of rice, few studies have investigated the mechanisms underlying these interactions. To investigate root morphological and physiological characteristics and determine yield and nitrogen use parameters, rhizo-box experiments were performed on rice using six treatments (no fertilizer, PK, N, NK, NP, and NPK) and plants were harvested at maturity. The aboveground biomass at the elongating stage and grain yield at maturity for NPK treatment were higher than the sum of PK and N treatments. N, P, and K interactions enhanced grain yield due to an increase in agronomic N use efficiency (NAE). The co-application of N, P, and K improved N uptake and N recovery efficiency, exceeding the decreases in physiological and internal NUE and thereby improving NAE. Increases in root length and biomass, N uptake per unit root length/root biomass, root oxidation activity, total roots absorption area, and roots active absorption area at the elongating stage improved N uptake via N, P, and K interactions. The higher total N uptake from N, P, and K interactions was due to improved root characteristics, which enhanced the rice yield and NUE.

Selection for nitrogen use efficiency and N concentration in timothy

1998 ◽  
Vol 78 (4) ◽  
pp. 611-613 ◽  
Author(s):  
R. Michaud ◽  
G. Bélanger ◽  
A. Brégard ◽  
J. Surprenant
Keyword(s):  
Nitrogen Use Efficiency ◽  
N Uptake ◽  
Genetic Selection ◽  
Phleum Pratense ◽  
N Use Efficiency ◽  
Nitrogen Use ◽  
N Concentration ◽  
Use Efficiency ◽  
Phleum Pratense L ◽  
N Use

Improvement of N use efficiency of timothy (Phleum pratense L.) involves higher DM yield but, for feeding purposes, N concentration should also be considered. Differences in N use efficiency were found among 40 genotypes of timothy. The analysis of residues of the linear regression between N uptake and DM yield of all genotypes allowed for the selection of genotypes with both high N use efficiency and contrasted N concentrations. Key words: Phleum pratense L., timothy, genetic selection, nitrogen use efficiency

scholarly journals Rice NIN-LIKE PROTEIN 3 plays a significant role in nitrogen use efficiency and grain yield under nitrate-sufficient conditions

2021 ◽  
Author(s):  
Zi-Sheng Zhang ◽  
Jin-Qiu Xia ◽  
Alamin Alfatih ◽  
Ying Song ◽  
Yi-Jie Huang ◽  
...  
Keyword(s):  
Grain Yield ◽  
Agricultural Production ◽  
Sufficient Conditions ◽  
Growth And Yield ◽  
Nuclear Retention ◽  
Nitrogen Use ◽  
Important Regulator ◽  
Use Efficiency ◽  
The Cost ◽  
N Use

AbstractNitrogen (N) is an essential macronutrient for crop growth and yield, but excessive application of N fertilizer has caused serious environmental pollution and greatly increased the cost of agricultural production. One of the effective and economical solutions to this dilemma is to improve the N use efficiency (NUE) of crops. Although some components involved in regulating NUE have been identified, the underlying molecular mechanism remains largely elusive in rice. Here we report that the OsNLP3 (NIN-like protein 3) is an important regulator in NUE and grain yield under nitrate-sufficient conditions. Both NUE and grain yield were significantly improved by enhanced OsNLP3 expression in the field, but reduced in osnlp3 mutants. The expression of OsNLP3 responds to both nitrate and ammonium, while OsNLP3 nuclear retention is only induced by nitrate, not by ammonium. OsNLP3 regulates the expression of a set of genes involved in N transport and assimilation by directly binding to the nitrate-responsive cis-element in the promoters of these genes. Our study demonstrates that OsNLP3 is significant for the regulation of NUE and grain yield, particularly in nitrate-rich conditions, thus providing a candidate for improving NUE and grain yield in rice.

Effects of nitrogen fertilization on grain protein content, nitrogen uptake, and nitrogen use efficiency of six spring wheat (Triticum aestivum L.) cultivars, in relation to estimated moisture supply

1992 ◽  
Vol 72 (1) ◽  
pp. 235-241 ◽  
Author(s):  
L. E. Gauer ◽  
C. A. Grant ◽  
L. D. Bailey ◽  
D. T. Gehl
Keyword(s):  
Triticum Aestivum ◽  
Protein Content ◽  
Spring Wheat ◽  
Nitrogen Fertilization ◽  
N Uptake ◽  
Triticum Aestivum L ◽  
Nitrogen Use ◽  
Moisture Supply ◽  
Use Efficiency ◽  
N Use

The effects of nitrogen fertilization on protein content, N uptake and N use efficiency of grain for six spring wheat cultivars were evaluated over a N application range of 0–200 kg ha−1, under two moisture supply levels, on Black Chernozemic soils in Manitoba. Moisture supply influenced protein content, protein yield, and grain N use efficiency (NUE) of applied fertilizer. Increased moisture supply lowered protein content and increased protein yield and NUE. Increasing N level increased protein, N uptake and decreased NUE, but effects depended on moisture supply. Cultivar differences occurred, especially at the higher moisture level.Key words: Protein, Triticum aestivum L., nitrogen uptake, nitrogen use efficiency, moisture

scholarly journals Rice NIN-LIKE PROTEIN 1 rapidly responds to nitrogen deficiency and improves yield and nitrogen use efficiency

2020 ◽  
Vol 71 (19) ◽  
pp. 6032-6042 ◽  
Author(s):  
Alamin Alfatih ◽  
Jie Wu ◽  
Zi-Sheng Zhang ◽  
Jin-Qiu Xia ◽  
Sami Ullah Jan ◽  
...  
Keyword(s):  
Grain Yield ◽  
Nitrogen Use Efficiency ◽  
Focal Point ◽  
Oryza Sativa L ◽  
N Uptake ◽  
Nitrogen Deficiency ◽  
Growth And Yield ◽  
Nitrogen Use ◽  
N Utilization ◽  
Use Efficiency

Abstract Nitrogen (N) is indispensable for crop growth and yield, but excessive agricultural application of nitrogenous fertilizers has generated severe environmental problems. A desirable and economical solution to cope with these issues is to improve crop nitrogen use efficiency (NUE). Plant NUE has been a focal point of intensive research worldwide, yet much still has to be learned about its genetic determinants and regulation. Here, we show that rice (Oryza sativa L.) NIN-LIKE PROTEIN 1 (OsNLP1) plays a fundamental role in N utilization. OsNLP1 protein localizes in the nucleus and its transcript level is rapidly induced by N starvation. Overexpression of OsNLP1 improves plant growth, grain yield, and NUE under different N conditions, while knockout of OsNLP1 impairs grain yield and NUE under N-limiting conditions. OsNLP1 regulates nitrate and ammonium utilization by cooperatively orchestrating multiple N uptake and assimilation genes. Chromatin immunoprecipitation and yeast one-hybrid assays showed that OsNLP1 can directly bind to the promoter of these genes to activate their expression. Therefore, our results demonstrate that OsNLP1 is a key regulator of N utilization and represents a potential target for improving NUE and yield in rice.

Engineering nitrogen use efficiency with alanine aminotransferase

2007 ◽  
Vol 85 (3) ◽  
pp. 252-262 ◽  
Author(s):  
Allen G. Good ◽  
Susan J. Johnson ◽  
Mary De Pauw ◽  
Rebecka T. Carroll ◽  
Nic Savidov ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Seed Yield ◽  
Nitrogen Use Efficiency ◽  
Nitrogen Fertilizer ◽  
Alanine Aminotransferase ◽  
Crop Productivity ◽  
Wild Type ◽  
Nitrogen Use ◽  
Use Efficiency ◽  
N Use

Nitrogen (N) is the most important factor limiting crop productivity worldwide. The ability of plants to acquire N from applied fertilizers is one of the critical steps limiting the efficient use of nitrogen. To improve N use efficiency, genetically modified plants that overexpress alanine aminotransferase (AlaAT) were engineered by introducing a barley AlaAT cDNA driven by a canola root specific promoter (btg26). Compared with wild-type canola, transgenic plants had increased biomass and seed yield both in the laboratory and field under low N conditions, whereas no differences were observed under high N. The transgenics also had increased nitrate influx. These changes resulted in a 40% decrease in the amount of applied nitrogen fertilizer required under field conditions to achieve yields equivalent to wild-type plants.

scholarly journals Performance of Prilled Urea and Urea Super Granule by Applicators on Yield and Nitrogen Use Efficiency in Boro Rice

2019 ◽  
Vol 22 (2) ◽  
pp. 63-69
Author(s):  
ATMS Hossain ◽  
F Rahman ◽  
PK Saha
Keyword(s):  
Grain Yield ◽  
Nitrogen Use Efficiency ◽  
N Uptake ◽  
N Use Efficiency ◽  
Nitrogen Use ◽  
N Application ◽  
Deep Placement ◽  
Prilled Urea ◽  
Use Efficiency ◽  
N Use

A field experiment was conducted on validation of prilled urea (PU) and urea super granule (USG) applied by applicators on yield and nitrogen use efficiency during Boro 2014 season at Bangladesh Rice Research Institute (BRRI) farm, Gazipur (AEZ 28). Six treatment combinations of different N doses and methods of N application were tested to compare urea-N application by PU and USG applicator for rice yield, N uptake and N use efficiency over urea broadcasting. Application of N as PU or USG through applicator has same effect on grain yield, N uptake and N use efficiency compared with urea broadcasting. Statistically similar grain yield were observed with N application as PU or USG @ 78 kg N ha-1 by applicator which was comparable with urea broadcasting @ 135 kg N ha-1. The N concentration and uptake in both panicle initiation (PI) and maturity stage were higher in USG deep placement than PU deep placement by applicators but the difference was not significant. Although agronomic use efficiency (AUE) of N was slightly higher in PU than USG applied by applicators but the recovery efficiency (RE) of N was higher in USG than PU. Bangladesh Rice j. 2018, 22(2): 63-69

scholarly journals Rice NIN-LIKE PROTEIN 1 Rapidly Responds to Nitrogen Deficiency and Improves Yield and Nitrogen Use Efficiency

2020 ◽  
Author(s):  
Alamin Alfatih ◽  
Jie Wu ◽  
Zi-Sheng Zhang ◽  
Jing-Qiu Xia ◽  
Sami Ullah Jan ◽  
...  
Keyword(s):  
Grain Yield ◽  
Nitrogen Use Efficiency ◽  
N Uptake ◽  
Nitrogen Deficiency ◽  
Growth And Yield ◽  
High Yield ◽  
N Availability ◽  
Nitrogen Use ◽  
N Utilization ◽  
Use Efficiency

ABSTRACTNitrogen (N) is indispensable for crop growth and yield, but excessive agricultural application of nitrogenous fertilizers has generated severe environmental problems. A desirable and economical solution to cope with these issues is to improve crop nitrogen use efficiency (NUE). Plant NUE has been a focal point of intensive research worldwide, yet much more has to be learned about its genetic determinants and regulation. Here, we show that rice NIN-LIKE PROTEIN 1 (OsNLP1) plays a fundamental role in N utilization. OsNLP1 protein localizes in nucleus and its transcript level is rapidly induced by N starvation. Overexpression of OsNLP1 improves plant growth, grain yield and NUE under different N conditions while knockout of OsNLP1 impairs grain yield and NUE under N limiting conditions. OsNLP1 regulates nitrate and ammonium utilization by cooperatively orchestrating multiple N uptake and assimilation genes. Chromatin immunoprecipitation and yeast-one-hybrid assays show that OsNLP1 can directly bind to the promoter of these genes to activate their expression. Therefore, our results demonstrate that OsNLP1 is a key regulator of N utilization and represents a potential target for improving NUE and yield in rice.One-sentence summaryOsNLP1 rapidly responds to N availability, enhances N uptake and assimilation, and holds great potential in promoting high yield in rice.

scholarly journals Genotypic Variation in Nitrogen Use-Efficiency Traits of 28 Tobacco Genotypes

Agronomy ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 572
Author(s):  
André B. Andrade ◽  
Douglas R. Guelfi ◽  
Valdemar Faquin ◽  
Fabrício S. Coelho ◽  
Carolina S. de C. Souza ◽  
...  
Keyword(s):  
Nitrogen Use Efficiency ◽  
N Uptake ◽  
Genotypic Variation ◽  
Sustainable Use ◽  
Utilization Efficiency ◽  
N Availability ◽  
Nitrogen Use ◽  
N Supply ◽  
Use Efficiency ◽  
N Use

Knowing the nitrogen use efficiency (NUE) of crops is crucial to minimize environmental pollution, although NUE is rarely provided for numerous genotypes in the tobacco (Nicotiana tabacum L.) crop. Through the growth of contrasting genotypes in nutritive solutions, we aimed to characterize five NUE components of 28 genotypes and to classify them according to their efficiency and responsiveness to nitrogen (N) availability. On average, physiological N use efficiency, N harvest index, and N uptake efficiency decreased by 16%, 4%, and 57%, respectively, under N-deficient conditions, while N utilization efficiency decreased by 43% at adequate N supply. The relative efficiency of N use varied from 35% to 59% among genotypes. All genotypes of the Virginia and Maryland varietal groups were efficient, and those of the Burley, Comum, and Dark groups were inefficient, while the responsiveness varied among genotypes within varietal groups, except for Maryland genotypes. Our findings are helpful in indicating genotypes with distinguished efficiency and responsiveness to N supply, which can be further chosen according to soil N level or affordability to N fertilizers worldwide in tobacco crops. In a general framework, this can lead to a more sustainable use of N and can support tobacco breeding programs for NUE.

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