scholarly journals Time-restricted feeding from adulthood to old age improves biconditional associative learning in geriatric rats regardless of macronutrient composition

2020 ◽  
Author(s):  
Abbi R. Hernandez ◽  
Quinten P. Federico ◽  
Sara N. Burke
Keyword(s):  
Dietary Intervention ◽  
Small Sample Size ◽  
Control Diet ◽  
Small Sample ◽  
Restricted Feeding ◽  
Macronutrient Composition ◽  
Mature Adult ◽  
Age Related ◽  
Feeding Regimen ◽  
Ad Libitum

AbstractDeclining health and cognition are hallmarks of advanced age that reduce both the quality and length of the lifespan. While caloric restriction has been highlighted as a dietary intervention capable of improving the healthspan by restoring metabolic function in late life, time-restricted feeding and changes in dietary macronutrient composition may be more feasible alternatives with similar health outcomes. To investigate the potential of these two interventions, a pilot cohort of fully mature adult rats were placed on a time-restricted feeding regimen of a ketogenic or micronutrient and calorically matched control diet from 8 to 21 months of age. A third group of rats was permitted to eat standard chow ad libitum. At 22 months, all rats were then placed on time-restricted feeding and tested on a biconditional association task. While the data presented here are preliminary (small sample size of 3-4/diet group), additional animals are currently undergoing the feeding regimen, and will be added into the behavioral studies at a later date. For the current data, regardless of dietary composition, time-restricted-fed rats performed significantly better than ad libitum-fed rats. This observation could not be accounted for by differences in motivation, procedural or sensorimotor impairments, indicating that mid-life dietary interventions capable of preventing metabolic impairments may also serve to prevent age-related cognitive decline.

scholarly journals THE INFLUENCE OF LIFE-EXTENDING MUTATION AND DIETARY INTERVENTION ON GUT MICROBIOTA

2019 ◽  
Vol 3 (Supplement_1) ◽  
pp. S834-S834
Author(s):  
Michal Masternak ◽  
Denise S Wiesenborn ◽  
Augusto Schneider ◽  
Till Strowig ◽  
Karl –Herbert Schafer ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Calorie Restriction ◽  
Dietary Intervention ◽  
Ecological System ◽  
Gastrointestinal Microbiota ◽  
Age Related ◽  
Ames Dwarf ◽  
Feeding Regimen ◽  
Ad Libitum ◽  
The Impact

Abstract The gastrointestinal microbiota represents a large and complex ecological system of different microorganisms. Recently, there is an increasing interest in the impact of microbiota on development of different age-related diseases. We tested the changes of gut microbiota during development in long-living Ames dwarf (df/df) mice and we compared the effects of this life-extending mutation with the impact of calorie restriction (CR). Importantly, the analysis of microbiome showed significant differences in the ratio of Bacteroidetes and Firmicutes when comparing df/df and normal (N) mice (p<0.001). The LefSe analysis showed distinct microbiome distribution between CR and ad libitum (AL) feeding regimen in N animals (p<0.004), yet there was lack of similar changes in response to CR in df/df mice. In summary, our study showed significant genotype impact on gut microbiota and we showed that life-extending CR regimen provide divergent effects on gut microbiota in N when comparing with df/df mice.

scholarly journals Influence of aging, macronutrient composition and time-restricted feeding on the rat gut microbiome

2020 ◽  
Vol 4 (Supplement_1) ◽  
pp. 888-888
Author(s):  
Abbi Hernandez ◽  
Sara Burke ◽  
Thomas Buford ◽  
Christy Carter
Keyword(s):  
Gut Microbiome ◽  
Control Diet ◽  
Diet Group ◽  
Therapeutic Interventions ◽  
Brown Norway ◽  
Restricted Feeding ◽  
Macronutrient Composition ◽  
Microbiome Composition ◽  
Low Carbohydrate Diet ◽  
Age Related

Abstract Declining health and cognition are hallmarks of advanced age that reduce both the quality and length of the lifespan. While caloric restriction has been highlighted as a strategy for increasing healthspan, time-restricted feeding and changes in dietary macronutrient composition may be more feasible alternatives with similar health outcomes. Furthermore, age-related changes in gut microbiome composition may reciprocally interact with several physiological systems – providing a good target for future therapeutic interventions. To begin to investigate the potential utility of a ketogenic (high fat, low carbohydrate) diet and/or time-restricted feeding, fully mature young (5 mo) and older (22 mo) adult male Fischer Brown Norway Hybrid rats were placed on a time-restricted feeding regimen of a ketogenic or micronutrient and calorically matched control diet for 7 months. A third group of rats was permitted to eat standard chow ad libitum. Fecal samples collected at the conclusion of the study were submitted for 16S microbiome analysis, which revealed significant differences across age and diet groups, as well as across feeding paradigms. Beta diversity analysis demonstrated distinct microbiome composition across the three diet groups regardless of age. Furthermore, diet group significantly impacted abundance in expression of several microbiota at the phylum level, including Verrucomicrobia, Cyanobacteria, Actinobacteria and Patescibacteria, though age did not. Verrucomicrobia was significantly increased (p=0.02) and Actinobacteria and Patescibacteria (p<0.01) were significantly decreased in animals fed in a time-restricted fashion. These results indicate the value of both altered macronutrient composition and altered feeding methodology for therapeutic interventions targeting the gut microbiome.

scholarly journals Blinding in electric current stimulation in subacute neglect patients with current densities of 0.8 A/m2: a cross-over pilot study

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Anna Gorsler ◽  
Ulrike Grittner ◽  
Nadine Külzow ◽  
Torsten Rackoll
Keyword(s):  
Pilot Study ◽  
Electric Fields ◽  
Standard Care ◽  
Small Sample Size ◽  
Small Sample ◽  
Related Factors ◽  
Attentional Deficits ◽  
Age Related ◽  
Current Densities ◽  
Improve Standard

Abstract Objective Neglect after stroke is a disabling disorder and its rehabilitation is a major challenge. Transcranial direct current stimulation (tDCS) seems to be a promising adjuvant technique to improve standard care neglect therapy. Since electric fields are influenced by age-related factors, higher current densities are probably needed for effective treatment in aged stroke patients. Validation of treatment efficacy requires sham-controlled experiments, but increased current densities might comprise blinding. Therefore, a pilot study was conducted to test sham adequacy when using current density of 0.8 A/m2. Whether especially neglect patients who mainly suffer from perceptual and attentional deficits are able to differentiate beyond chance active from sham tDCS was investigated in a randomized cross-over design (active/sham stimulation) in 12 early subacute patients with left-sided hemineglect. Stimulation (0.8 A/m2) was performed simultaneous to standard care neglect therapy. Results Odds ratio of correct guessing an atDCS condition compared to wrongly judge an atDCS condition as sham was 10.00 (95%CI 0.65–154.40, p = 0.099). However, given the small sample size and high OR, although likely somewhat overestimated, results require careful interpretation and blinding success in neglect studies with current densities of 0.8 A/m2 should be further confirmed.

scholarly journals Effects of Age and Serum 25-OH-Vitamin D on Serum Parathyroid Hormone Levels

2012 ◽  
Vol 97 (11) ◽  
pp. 3989-3995 ◽  
Author(s):  
A. Valcour ◽  
F. Blocki ◽  
D. M. Hawkins ◽  
Sudhaker D. Rao
Keyword(s):  
Vitamin D ◽  
Calcium Absorption ◽  
Small Sample Size ◽  
International Workshop ◽  
Large Population ◽  
Small Sample ◽  
Reference Laboratory ◽  
Population Database ◽  
Age Related ◽  
Serum Pth

Context: Several studies define optimal serum 25-hydroxyvitamin D (25-OHD) levels based on serum PTH level reaching an asymptote. However, results differ widely, ranging from 25-OHD levels of 12–44 ng/ml: many studies are constrained by small sample size. Objective: The objective of the study was to determine the relationship between serum PTH and 25-OHD levels and age in a very large reference laboratory database. Design: This was a detailed cross-sectional analysis of 312,962 paired serum PTH and 25-OHD levels measured from July 2010 to June 2011. Results: Median PTH levels and the proportion of patients (PTH > 65 pg/ml), from 63 successive 25-OHD frequency classes of 5000 patients, provide smooth, exceptionally well-fitted curves (R2 = 0.994 and R2 = 0.995, respectively) without discernible inflection points or asymptotes but with striking age dependencies. Serum 25-OHD was below the recent Institute of Medicine sufficiency guidance of 20 ng/ml in 27% (85,000) of the subjects. More importantly, 40 and 51% of subjects (serum 25-OHD <20 and 10 ng/ml, respectively) had biochemical hyperparathyroidism (PTH > 65 pg/ml). Conclusions: This analysis, despite inevitable inherent limitations, introduces several clinical implications. First, median 25-OHD-dependent PTH levels revealed no threshold above which increasing 25-OHD fails to further suppress PTH. Second, the large number of subjects with 25-OHD deficiency and hyperparathyroidism reinforces the Third International Workshop on Asymptomatic Primary Hyper parathyroidism's recommendations to test for, and replete, vitamin D depletion before considering parathyroidectomy. Third, strong age dependency of the PTH-25-OHD relationship likely reflects the composite effects of age-related decline in calcium absorption and renal function. Finally, this unselected large population database study could guide clinical management of patients based on an age-dependent, PTH-25-OHD continuum.

scholarly journals Long-Term Feeding of a High-Fat Diet Ameliorated Age-Related Phenotypes in SAMP8 Mice

Nutrients ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1416
Author(s):  
Hideaki Oike ◽  
Yukino Ogawa ◽  
Kayo Azami
Keyword(s):  
Control Diet ◽  
Active Phase ◽  
Restricted Feeding ◽  
Long Term Effects ◽  
High Fat ◽  
Night Time ◽  
Age Related ◽  
Nutritional Studies ◽  
Samp8 Mice

High-fat diets (HFD) have been thought to increase the risk of obesity and metabolic syndrome, as well as shorten lifespan. On the other hand, chrono-nutritional studies have shown that time-restricted feeding during active phase significantly suppresses the induction of HFD-induced obesity in mouse model. However, the long-term effects of time-restricted HFD feeding on aging are unknown. Therefore, in this study, we set up a total of four groups: mutual combination of ad libitum feeding or night-time-restricted feeding (NtRF) and an HFD or a control diet. We examined their long-term effects in a senescence-accelerated mouse strain, SAMP8, for over a year. Hearing ability, cognitive function, and other behavioral and physiological indexes were evaluated during the study. Unexpectedly, SAMP8 mice did not show early onset of death caused by the prolonged HFD intake, and both HFD and NtRF retarded age-related hearing loss (AHL). NtRF improved grip strength and cognitive memory scores, while HFD weakly suppressed age-related worsening of the appearance scores associated with the eyes. Notably, the HFD also retarded the progression of AHL in both DBA/2J and C57BL/6J mice. These results suggest that HFD prevents aging unless metabolic disorders occur and that HFD and NtRF are independently effective in retarding aging; thus, the combination of HFD and chrono-nutritional feeding may be an effective anti-aging strategy.

Optical coherence tomography angiography in amblyopia: A critical update on current understandings and future perspectives

2021 ◽  
pp. 112067212110425
Author(s):  
Amar Pujari ◽  
Gunjan Saluja ◽  
Rohan Chawla ◽  
Asha Samdani ◽  
Swati Phuljhele ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Optical Coherence Tomography Angiography ◽  
Small Sample Size ◽  
Small Sample ◽  
Age Related Macular Degeneration ◽  
Optical Coherence ◽  
Patient Profile ◽  
Age Related ◽  
Choroidal Vasculature

Optical coherence tomography angiography (OCTA) is a non-invasive tool to assess the retino-choroidal vasculature in vivo. It tracks the red blood cell movement and maps the vasculature in quick succession. In routine, diabetic retinopathy, age related macular degeneration, central serous chorioretinopathy, and others are commonly being studied to unveil its clinic role. On the other hand, amblyopia is a condition where the visual acuity is subnormal due to non-organic causes in the eye. But the OCTA studies till now have shown variable changes along retino-choroidal vasculature. Hence, to comprehend the existing literature knowledge, a systematic literature search was carried out and the original works describing novel findings in amblyopic eyes on OCTA were included. Upon detailed assessment, firstly, the disturbed vasculature along superficial retinal plexus, deeper retinal plexus, and choroidal plexus were evident in most untreated amblyopic eyes. However, such changes were not uniform, which is due to noted heterogenic patient profile, small sample size, biometric biases, non-uniform algorithms, and other factors. And to note, even in presence of such diverse changes, almost all the authors stated a plausible explanation for their notable changes. Secondly, the utility of OCTA in identifying vascular changes with standard treatments and segregation of visual beneficiaries from non-beneficiaries were possible. Hence, to conclude, OCTA is a valuable tool which can provide valuable useful insights into the amblyopic eyes during pre and post treatment periods. However, to gather more concrete evidence for clinical benefits, systematic, homogenous, and better structured clinical studies are mandated.

The effects of genotype, sex and feeding regimen on pig carcass development: 1. Primary components, tissues and joints

1979 ◽  
Vol 93 (2) ◽  
pp. 339-347 ◽  
Author(s):  
D. G. Evans ◽  
A. J. Kempster
Keyword(s):  
Growth Period ◽  
Carcass Weight ◽  
Restricted Feeding ◽  
Alternative Models ◽  
Lean Growth ◽  
Feeding Regimen ◽  
Ad Libitum ◽  
Pig Carcasses

SUMMARYDissection data for 1006 pig carcasses taken from the first 2 years of the Meat and Livestock Commission's (MLC) Commercial Pig Evaluation (CPE) were used to examine the relationships between carcass components and carcass weight over the growth period from 46 to 92 kg carcass weight. Alternative models were tested and parameters examined for different genotypes (pigs from different companies in CPE), sexes (barrows and gilts), feeding regimens (ad libitumand restricted feeding) and years.Generally, linear allometry provided an acceptable description of carcass development, although some curvilinearity was indicated for the head, kidneys, rump back joint, M. psoas and total dissected lean. Growth coefficients conformed to the generally accepted pattern of carcass development in pigs. Using linear allometry, proportional weight increases were comparable for all genotypes and sexes, but dissected fat increased relatively faster on the restricted feeding regimen.At a constant carcass weight of 66·5 kg, all tissue weights and the weights of primary components and most joints differed by genotype. The ratio of carcass lean weights for the two extreme genotypes was 1·04; comparable ratios for carcass fat weights were 1·13 (restricted-fed pigs) and 1·07 (ad libitum-fed pigs).Significant differences were recorded between sexes and between feeding regimens, largely reflecting differences in fatness. Some important year differences were also found.

Effect of food intake capacity on genotype by feeding regimen interactions in growing pigs

1990 ◽  
Vol 50 (2) ◽  
pp. 343-351 ◽  
Author(s):  
E. Kanis
Keyword(s):  
Growth Rate ◽  
Food Intake ◽  
Live Weight ◽  
Tissue Growth ◽  
Growing Pigs ◽  
Fatty Tissue ◽  
Restricted Feeding ◽  
Production Traits ◽  
Feeding Regimen ◽  
Ad Libitum

ABSTRACTFrom each of 159 litters, between one and three pigs were fed ad libitum and between one and four pigs were fed at several restricted feeding levels from about 27 to 107 kg live weight. Effects of litter by feeding regimen (ad libitumv. restricted feeding) interactions (L × F) and sex by feeding regimen interactions (S × F) on production traits were investigated. In experimental groups where restrictedly fed animals received an amount of food based on their estimated voluntary food intake capacity (FIC), interactions were non-significant. In groups where restricted feeding was independent of FIC, significant L × F or S × F interactions were found for daily food intake (FI), daily gain, lean tissue growth rate and fatty tissue growth rate, but not for body composition and food conversion traits.Because of between-animal variation in FIC, restricted feeding according to a fixed scale restricted intake of some animals to a higher degree than others and degree of food intake restriction (DFR) was calculated from actual FI and FIC for animals fed at a restricted level. After correction of FI and production traits of restrictedly fed animals for differences in DFR, L × F and S × F interactions were not significant.It was concluded that the poor relationships often found between test-station results of boars and results of their progeny in practical environments may be caused, to an important extent, by differences in DFR in each environment. To overcome these genotype by environment interactions it is recommended that animals be fed ad libitum in both test and commercial environments.

scholarly journals Can Night Shift Work Affect Biological Age? Hints from a Cross-Sectional Study on Hospital Female Nurses

2021 ◽  
Vol 18 (20) ◽  
pp. 10639
Author(s):  
Michele Carugno ◽  
Cristina Maggioni ◽  
Vincenzo Ruggiero ◽  
Eleonora Crespi ◽  
Paola Monti ◽  
...  
Keyword(s):  
Small Sample Size ◽  
Night Shift ◽  
Cross Sectional Study ◽  
Small Sample ◽  
Biological Age ◽  
Linear Regression Models ◽  
Cross Sectional ◽  
Work Related ◽  
Age Related ◽  
Female Nurses

Disruption of age-related processes seems to play a relevant role in health effects related to night shift (NS) work. We aim to verify whether NS work can influence biological age (BA), estimated through Zbieć-Piekarska’s epigenetic signature, based on methylation of five CpG sites in ELOVL2, C1orf132/MIR29B2C, TRIM59, KLF14, and FHL2. Forty-six female nurses working in NS were matched by age and length of employment with 51 female colleagues not working in NS. Each subject filled in a questionnaire (including the Effort Reward Imbalance (ERI) index to assess job stress) and gave a blood sample. Age acceleration (AA) was estimated by regressing BA on chronological age and taking the residuals. Multivariate linear regression models were applied. BA was not associated with NS. However, we did observe an increase in AA per each year in NS in subjects with overweight/obesity (β = 0.46, 95%CI: 0.05; 0.87, p = 0.03), experiencing work-related stress (β = 0.58, 95% CI: 0.10; 1.06, p = 0.018), or both (β = 0.66, 95% CI: 0.03; 1.29, p = 0.041). Although based on a small sample size, our findings suggest an increased BA only among hypersusceptible subjects and is worth further investigation, also in light of recent results suggesting a higher breast cancer risk in women with increased AA.

scholarly journals Blinding in electric current stimulation in subacute neglect patients with current densities of 0.8 A/m²: A cross-over pilot study

2020 ◽  
Author(s):  
Anna Gorsler ◽  
Ulrike Grittner ◽  
Nadine Külzow ◽  
Torsten Rackoll
Keyword(s):  
Pilot Study ◽  
Electric Fields ◽  
Standard Care ◽  
Small Sample Size ◽  
Small Sample ◽  
Related Factors ◽  
Attentional Deficits ◽  
Age Related ◽  
Current Densities ◽  
Improve Standard

Abstract Objective Neglect after stroke is a disabling disorder and its rehabilitation is a major challenge. Transcranial direct current stimulation (tDCS) seems to be a promising adjuvant technique to improve standard care neglect therapy. Since electric fields are influenced by age-related factors, higher current densities are probably needed for effective treatment in aged stroke patients. Validation of treatment efficacy requires sham-controlled experiments, but increased current densities might comprise blinding. Therefore, a pilot study was conducted to test sham adequacy when using current density of 0.8 A/m2. Whether especially neglect patients who mainly suffer from perceptual and attentional deficits are able to differentiate beyond chance active from sham tDCS was investigated in a randomized cross-over design (active/sham stimulation) in 12 early subacute patients with left-sided hemineglect. Stimulation (0.8 A/m²) was performed simultaneous to standard care neglect therapy. Results Odds ratio of correct guessing an atDCS condition compared to wrongly judge an atDCS condition as sham was 10.00 (95%CI: 0.65 - 154.40, p = 0.099). However, given the small sample size and high OR, although likely somewhat overestimated, results require careful interpretation and blinding success in neglect studies with current densities of 0.8 A/m² should be further confirmed.

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